This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.70 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 382,570 (14.4%) Reads that were too short: 18,934 (0.7%) Reads written (passing filters): 363,636 (13.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 94,219,856 bp (11.8%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 382570 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1134 649.0 0 1134 7 36934 649.0 0 36934 8 38721 649.0 0 38721 9 249 649.0 0 249 10 224 649.0 0 224 11 122 649.0 0 122 12 138 649.0 0 138 13 344 649.0 0 344 14 394 649.0 0 394 15 345 649.0 0 345 16 293 649.0 0 293 17 555 649.0 0 555 18 644 649.0 0 644 19 1004 649.0 0 1004 20 25844 649.0 0 25844 21 17174 649.0 0 17174 22 30925 649.0 0 30925 23 198 649.0 0 198 24 129 649.0 0 129 25 157 649.0 0 157 26 93 649.0 0 93 27 104 649.0 0 104 28 96 649.0 0 96 29 93 649.0 0 93 30 67 649.0 0 67 31 90 649.0 0 90 32 170 649.0 0 170 33 251 649.0 0 251 34 268 649.0 0 268 35 595 649.0 0 595 36 1231 649.0 0 1231 37 337 649.0 0 337 38 369 649.0 0 369 39 803 649.0 0 803 40 360 649.0 0 360 41 353 649.0 0 353 42 654 649.0 0 654 43 654 649.0 0 654 44 743 649.0 0 743 45 740 649.0 0 740 46 1178 649.0 0 1178 47 1428 649.0 0 1428 48 3054 649.0 0 3054 49 65587 649.0 0 65587 50 43881 649.0 0 43881 51 60304 649.0 0 60304 52 226 649.0 0 226 53 42 649.0 0 42 54 30 649.0 0 30 55 38 649.0 0 38 56 45 649.0 0 45 57 50 649.0 0 50 58 68 649.0 0 68 59 33 649.0 0 33 60 71 649.0 0 71 61 43 649.0 0 43 62 78 649.0 0 78 63 376 649.0 0 376 64 280 649.0 0 280 65 715 649.0 0 715 66 35 649.0 0 35 67 50 649.0 0 50 68 45 649.0 0 45 69 34 649.0 0 34 70 34 649.0 0 34 71 36 649.0 0 36 72 42 649.0 0 42 73 43 649.0 0 43 74 46 649.0 0 46 75 35 649.0 0 35 76 34 649.0 0 34 77 36 649.0 0 36 78 30 649.0 0 30 79 45 649.0 0 45 80 33 649.0 0 33 81 35 649.0 0 35 82 20 649.0 0 20 83 51 649.0 0 51 84 42 649.0 0 42 85 39 649.0 0 39 86 42 649.0 0 42 87 44 649.0 0 44 88 60 649.0 0 60 89 35 649.0 0 35 90 52 649.0 0 52 91 36 649.0 0 36 92 46 649.0 0 46 93 57 649.0 0 57 94 37 649.0 0 37 95 34 649.0 0 34 96 33 649.0 0 33 97 29 649.0 0 29 98 49 649.0 0 49 99 30 649.0 0 30 100 28 649.0 0 28 101 40 649.0 0 40 102 44 649.0 0 44 103 21 649.0 0 21 104 26 649.0 0 26 105 32 649.0 0 32 106 62 649.0 0 62 107 47 649.0 0 47 108 21 649.0 0 21 109 30 649.0 0 30 110 41 649.0 0 41 111 43 649.0 0 43 112 60 649.0 0 60 113 42 649.0 0 42 114 32 649.0 0 32 115 69 649.0 0 69 116 47 649.0 0 47 117 126 649.0 0 126 118 125 649.0 0 125 119 149 649.0 0 149 120 54 649.0 0 54 121 44 649.0 0 44 122 48 649.0 0 48 123 53 649.0 0 53 124 513 649.0 0 513 125 117 649.0 0 117 126 250 649.0 0 250 127 52 649.0 0 52 128 44 649.0 0 44 129 41 649.0 0 41 130 52 649.0 0 52 131 24 649.0 0 24 132 47 649.0 0 47 133 43 649.0 0 43 134 55 649.0 0 55 135 44 649.0 0 44 136 155 649.0 0 155 137 49 649.0 0 49 138 48 649.0 0 48 139 58 649.0 0 58 140 40 649.0 0 40 141 53 649.0 0 53 142 63 649.0 0 63 143 56 649.0 0 56 144 78 649.0 0 78 145 57 649.0 0 57 146 65 649.0 0 65 147 43 649.0 0 43 148 32 649.0 0 32 149 49 649.0 0 49 150 60 649.0 0 60 151 41 649.0 0 41 152 52 649.0 0 52 153 60 649.0 0 60 154 46 649.0 0 46 155 47 649.0 0 47 156 45 649.0 0 45 157 53 649.0 0 53 158 62 649.0 0 62 159 67 649.0 0 67 160 51 649.0 0 51 161 54 649.0 0 54 162 51 649.0 0 51 163 33 649.0 0 33 164 38 649.0 0 38 165 51 649.0 0 51 166 40 649.0 0 40 167 53 649.0 0 53 168 49 649.0 0 49 169 49 649.0 0 49 170 63 649.0 0 63 171 52 649.0 0 52 172 49 649.0 0 49 173 55 649.0 0 55 174 38 649.0 0 38 175 63 649.0 0 63 176 75 649.0 0 75 177 60 649.0 0 60 178 81 649.0 0 81 179 121 649.0 0 121 180 194 649.0 0 194 181 333 649.0 0 333 182 5533 649.0 0 5533 183 511 649.0 0 511 184 2588 649.0 0 2588 185 59 649.0 0 59 186 54 649.0 0 54 187 47 649.0 0 47 188 70 649.0 0 70 189 126 649.0 0 126 190 238 649.0 0 238 191 117 649.0 0 117 192 119 649.0 0 119 193 1178 649.0 0 1178 194 1820 649.0 0 1820 195 1971 649.0 0 1971 196 668 649.0 0 668 197 191 649.0 0 191 198 119 649.0 0 119 199 35 649.0 0 35 200 67 649.0 0 67 201 52 649.0 0 52 202 58 649.0 0 58 203 48 649.0 0 48 204 55 649.0 0 55 205 152 649.0 0 152 206 70 649.0 0 70 207 52 649.0 0 52 208 71 649.0 0 71 209 65 649.0 0 65 210 52 649.0 0 52 211 72 649.0 0 72 212 61 649.0 0 61 213 168 649.0 0 168 214 592 649.0 0 592 215 318 649.0 0 318 216 474 649.0 0 474 217 97 649.0 0 97 218 85 649.0 0 85 219 170 649.0 0 170 220 214 649.0 0 214 221 218 649.0 0 218 222 83 649.0 0 83 223 74 649.0 0 74 224 56 649.0 0 56 225 81 649.0 0 81 226 68 649.0 0 68 227 86 649.0 0 86 228 57 649.0 0 57 229 52 649.0 0 52 230 48 649.0 0 48 231 71 649.0 0 71 232 68 649.0 0 68 233 104 649.0 0 104 234 153 649.0 0 153 235 40 649.0 0 40 236 122 649.0 0 122 237 64 649.0 0 64 238 80 649.0 0 80 239 334 649.0 0 334 240 452 649.0 0 452 241 344 649.0 0 344 242 229 649.0 0 229 243 36 649.0 0 36 244 57 649.0 0 57 245 51 649.0 0 51 246 73 649.0 0 73 247 63 649.0 0 63 248 71 649.0 0 71 249 118 649.0 0 118 250 347 649.0 0 347 251 466 649.0 0 466 252 651 649.0 0 651 253 731 649.0 0 731 254 66 649.0 0 66 255 61 649.0 0 61 256 86 649.0 0 86 257 128 649.0 0 128 258 96 649.0 0 96 259 131 649.0 0 131 260 82 649.0 0 82 261 107 649.0 0 107 262 115 649.0 0 115 263 601 649.0 0 601 264 299 649.0 0 299 265 666 649.0 0 666 266 174 649.0 0 174 267 106 649.0 0 106 268 110 649.0 0 110 269 88 649.0 0 88 270 105 649.0 0 105 271 79 649.0 0 79 272 116 649.0 0 116 273 50 649.0 0 50 274 71 649.0 0 71 275 95 649.0 0 95 276 63 649.0 0 63 277 73 649.0 0 73 278 46 649.0 0 46 279 50 649.0 0 50 280 57 649.0 0 57 281 70 649.0 0 70 282 88 649.0 0 88 283 169 649.0 0 169 284 98 649.0 0 98 285 160 649.0 0 160 286 113 649.0 0 113 287 166 649.0 0 166 288 1740 649.0 0 1740 289 1278 649.0 0 1278 290 1326 649.0 0 1326 291 579 649.0 0 579 292 65 649.0 0 65 293 89 649.0 0 89 294 101 649.0 0 101 295 75 649.0 0 75 296 64 649.0 0 64 297 115 649.0 0 115 298 85 649.0 0 85 299 97 649.0 0 97 300 171 649.0 0 171 301 142 649.0 0 142 Finished in 15.20 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 363,636 Reads with adapters: 39,029 (10.7%) Reads that were too short: 451 (0.1%) Reads written (passing filters): 38,578 (10.6%) Total basepairs processed: 94,219,856 bp Total written (filtered): 10,230,096 bp (10.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 39029 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 10 16 264 0.0 3 0 258 6 17 35120 0.0 3 34382 718 18 2 18 92 0.0 3 4 86 2 19 12 0.0 3 0 0 12 20 50 0.0 3 1 0 0 49 21 1 0.0 3 0 0 0 1 22 2 0.0 3 0 0 0 2 23 4 0.0 3 0 0 0 4 26 1 0.0 3 0 0 0 1 28 2 0.0 3 1 0 0 1 30 1 0.0 3 0 0 0 1 34 7 0.0 3 0 0 2 5 38 2 0.0 3 1 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 5 0.0 3 0 0 0 5 49 12 0.0 3 0 0 0 12 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 57 12 0.0 3 0 0 0 12 59 1 0.0 3 0 0 0 1 60 4 0.0 3 0 0 0 4 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 203 0.0 3 0 0 0 203 74 8 0.0 3 0 0 0 8 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 12 0.0 3 0 0 0 12 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 7 0.0 3 0 0 1 6 92 2 0.0 3 0 0 0 2 93 59 0.0 3 0 0 56 3 94 13 0.0 3 0 0 0 13 95 1 0.0 3 0 0 0 1 96 1 0.0 3 1 97 24 0.0 3 0 0 0 24 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 101 12 0.0 3 0 0 1 11 102 20 0.0 3 0 0 0 20 103 3 0.0 3 0 0 0 3 104 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 2 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 17 0.0 3 0 0 0 17 119 3 0.0 3 0 0 0 3 120 5 0.0 3 0 0 0 5 121 9 0.0 3 0 0 0 9 122 201 0.0 3 0 0 1 200 123 335 0.0 3 0 0 1 334 124 223 0.0 3 0 0 1 222 125 51 0.0 3 0 0 0 51 126 77 0.0 3 0 0 2 75 127 36 0.0 3 0 0 2 34 128 66 0.0 3 0 0 1 65 129 17 0.0 3 0 0 0 17 130 14 0.0 3 0 0 0 14 131 8 0.0 3 0 0 0 8 132 2 0.0 3 0 0 0 2 133 6 0.0 3 0 0 1 5 134 2 0.0 3 0 0 0 2 135 4 0.0 3 0 0 0 4 137 19 0.0 3 0 0 1 18 138 9 0.0 3 0 0 0 9 139 15 0.0 3 0 0 0 15 140 4 0.0 3 0 0 0 4 142 158 0.0 3 0 0 1 157 143 13 0.0 3 0 0 0 13 144 3 0.0 3 1 0 0 2 145 16 0.0 3 0 0 0 16 146 21 0.0 3 0 0 0 21 147 4 0.0 3 0 0 0 4 148 6 0.0 3 0 0 0 6 149 8 0.0 3 0 0 0 8 150 4 0.0 3 0 0 0 4 151 50 0.0 3 0 0 0 50 152 32 0.0 3 0 0 0 32 153 21 0.0 3 0 0 0 21 154 9 0.0 3 0 0 0 9 155 9 0.0 3 0 0 1 8 156 3 0.0 3 0 0 0 3 157 4 0.0 3 0 0 0 4 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 166 4 0.0 3 0 0 0 4 167 5 0.0 3 0 0 0 5 168 1 0.0 3 0 0 0 1 169 3 0.0 3 0 0 0 3 170 715 0.0 3 0 0 4 711 171 130 0.0 3 0 0 0 130 172 120 0.0 3 0 0 0 120 173 3 0.0 3 0 0 0 3 174 36 0.0 3 0 0 0 36 175 14 0.0 3 0 0 1 13 177 19 0.0 3 0 0 1 18 178 1 0.0 3 0 0 0 1 179 5 0.0 3 0 0 0 5 180 76 0.0 3 0 0 1 75 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 188 2 0.0 3 0 0 0 2 189 2 0.0 3 0 0 0 2 190 9 0.0 3 0 0 0 9 192 1 0.0 3 0 0 0 1 193 1 0.0 3 1 195 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 3 0.0 3 0 0 0 3 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 13 0.0 3 1 0 0 12 203 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 8 0.0 3 0 0 0 8 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 223 3 0.0 3 0 0 0 3 224 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 3 0.0 3 0 0 0 3 230 1 0.0 3 0 0 0 1 231 3 0.0 3 0 0 0 3 232 2 0.0 3 0 0 0 2 233 3 0.0 3 0 0 0 3 234 12 0.0 3 0 0 0 12 235 45 0.0 3 0 0 0 45 236 23 0.0 3 0 0 0 23 237 25 0.0 3 0 0 1 24 238 68 0.0 3 0 0 2 66 239 10 0.0 3 0 0 1 9 240 4 0.0 3 0 0 0 4 241 6 0.0 3 0 0 0 6 242 3 0.0 3 0 0 0 3 243 7 0.0 3 0 0 0 7 244 25 0.0 3 0 0 1 24 245 1 0.0 3 0 0 0 1 246 4 0.0 3 0 0 0 4 248 2 0.0 3 0 0 0 2 249 7 0.0 3 0 0 0 7 250 9 0.0 3 0 0 0 9 251 1 0.0 3 0 0 0 1 252 3 0.0 3 0 0 0 3 253 1 0.0 3 0 0 0 1 254 3 0.0 3 0 0 0 3 257 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 7 0.0 3 0 0 1 6 264 4 0.0 3 0 0 0 4 265 1 0.0 3 0 0 0 1 266 3 0.0 3 0 0 0 3 267 4 0.0 3 0 0 0 4 268 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 6 0.0 3 0 0 0 6 273 7 0.0 3 0 0 0 7 277 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 38,578 Reads with adapters: 380 (1.0%) Reads written (passing filters): 38,578 (100.0%) Total basepairs processed: 10,230,096 bp Total written (filtered): 10,228,922 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 380 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.4% C: 14.7% G: 28.2% T: 13.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 350 602.8 0 350 4 29 150.7 0 29 8 1 0.6 0 1