This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.80 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 435,960 (16.4%) Reads that were too short: 31,129 (1.2%) Reads written (passing filters): 404,831 (15.2%) Total basepairs processed: 800,111,879 bp Total written (filtered): 98,756,961 bp (12.3%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 435960 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6498 649.0 0 6498 7 81207 649.0 0 81207 8 2615 649.0 0 2615 9 90253 649.0 0 90253 10 165 649.0 0 165 11 175 649.0 0 175 12 77 649.0 0 77 13 90 649.0 0 90 14 48 649.0 0 48 15 55 649.0 0 55 16 66 649.0 0 66 17 69 649.0 0 69 18 64 649.0 0 64 19 60 649.0 0 60 20 89 649.0 0 89 21 102 649.0 0 102 22 70 649.0 0 70 23 52 649.0 0 52 24 96 649.0 0 96 25 66 649.0 0 66 26 35 649.0 0 35 27 56 649.0 0 56 28 62 649.0 0 62 29 60 649.0 0 60 30 44 649.0 0 44 31 54 649.0 0 54 32 58 649.0 0 58 33 59 649.0 0 59 34 46 649.0 0 46 35 71 649.0 0 71 36 87 649.0 0 87 37 69 649.0 0 69 38 60 649.0 0 60 39 75 649.0 0 75 40 79 649.0 0 79 41 62 649.0 0 62 42 76 649.0 0 76 43 89 649.0 0 89 44 72 649.0 0 72 45 219 649.0 0 219 46 64 649.0 0 64 47 79 649.0 0 79 48 96 649.0 0 96 49 59 649.0 0 59 50 51 649.0 0 51 51 78 649.0 0 78 52 73 649.0 0 73 53 143 649.0 0 143 54 119 649.0 0 119 55 106 649.0 0 106 56 134 649.0 0 134 57 123 649.0 0 123 58 67 649.0 0 67 59 79 649.0 0 79 60 103 649.0 0 103 61 97 649.0 0 97 62 185 649.0 0 185 63 87 649.0 0 87 64 79 649.0 0 79 65 88 649.0 0 88 66 103 649.0 0 103 67 117 649.0 0 117 68 128 649.0 0 128 69 174 649.0 0 174 70 225 649.0 0 225 71 253 649.0 0 253 72 544 649.0 0 544 73 1127 649.0 0 1127 74 356 649.0 0 356 75 424 649.0 0 424 76 734 649.0 0 734 77 387 649.0 0 387 78 428 649.0 0 428 79 796 649.0 0 796 80 1141 649.0 0 1141 81 1401 649.0 0 1401 82 1057 649.0 0 1057 83 1863 649.0 0 1863 84 2103 649.0 0 2103 85 3425 649.0 0 3425 86 67945 649.0 0 67945 87 38458 649.0 0 38458 88 61137 649.0 0 61137 89 263 649.0 0 263 90 69 649.0 0 69 91 55 649.0 0 55 92 48 649.0 0 48 93 62 649.0 0 62 94 64 649.0 0 64 95 61 649.0 0 61 96 71 649.0 0 71 97 65 649.0 0 65 98 55 649.0 0 55 99 59 649.0 0 59 100 55 649.0 0 55 101 66 649.0 0 66 102 83 649.0 0 83 103 267 649.0 0 267 104 109 649.0 0 109 105 97 649.0 0 97 106 75 649.0 0 75 107 42 649.0 0 42 108 48 649.0 0 48 109 48 649.0 0 48 110 40 649.0 0 40 111 56 649.0 0 56 112 63 649.0 0 63 113 39 649.0 0 39 114 61 649.0 0 61 115 67 649.0 0 67 116 51 649.0 0 51 117 60 649.0 0 60 118 67 649.0 0 67 119 71 649.0 0 71 120 70 649.0 0 70 121 55 649.0 0 55 122 88 649.0 0 88 123 318 649.0 0 318 124 696 649.0 0 696 125 468 649.0 0 468 126 342 649.0 0 342 127 319 649.0 0 319 128 75 649.0 0 75 129 118 649.0 0 118 130 115 649.0 0 115 131 209 649.0 0 209 132 139 649.0 0 139 133 118 649.0 0 118 134 137 649.0 0 137 135 85 649.0 0 85 136 112 649.0 0 112 137 100 649.0 0 100 138 118 649.0 0 118 139 128 649.0 0 128 140 169 649.0 0 169 141 254 649.0 0 254 142 217 649.0 0 217 143 379 649.0 0 379 144 4620 649.0 0 4620 145 1963 649.0 0 1963 146 3534 649.0 0 3534 147 480 649.0 0 480 148 87 649.0 0 87 149 124 649.0 0 124 150 123 649.0 0 123 151 93 649.0 0 93 152 67 649.0 0 67 153 62 649.0 0 62 154 106 649.0 0 106 155 91 649.0 0 91 156 92 649.0 0 92 157 90 649.0 0 90 158 65 649.0 0 65 159 66 649.0 0 66 160 68 649.0 0 68 161 63 649.0 0 63 162 70 649.0 0 70 163 83 649.0 0 83 164 98 649.0 0 98 165 143 649.0 0 143 166 759 649.0 0 759 167 1117 649.0 0 1117 168 544 649.0 0 544 169 119 649.0 0 119 170 98 649.0 0 98 171 172 649.0 0 172 172 170 649.0 0 170 173 112 649.0 0 112 174 84 649.0 0 84 175 113 649.0 0 113 176 100 649.0 0 100 177 78 649.0 0 78 178 114 649.0 0 114 179 87 649.0 0 87 180 134 649.0 0 134 181 147 649.0 0 147 182 175 649.0 0 175 183 201 649.0 0 201 184 325 649.0 0 325 185 4874 649.0 0 4874 186 1416 649.0 0 1416 187 3338 649.0 0 3338 188 129 649.0 0 129 189 64 649.0 0 64 190 58 649.0 0 58 191 72 649.0 0 72 192 144 649.0 0 144 193 152 649.0 0 152 194 111 649.0 0 111 195 127 649.0 0 127 196 137 649.0 0 137 197 95 649.0 0 95 198 87 649.0 0 87 199 140 649.0 0 140 200 152 649.0 0 152 201 146 649.0 0 146 202 196 649.0 0 196 203 230 649.0 0 230 204 345 649.0 0 345 205 541 649.0 0 541 206 12510 649.0 0 12510 207 2244 649.0 0 2244 208 3701 649.0 0 3701 209 97 649.0 0 97 210 90 649.0 0 90 211 389 649.0 0 389 212 344 649.0 0 344 213 570 649.0 0 570 214 78 649.0 0 78 215 45 649.0 0 45 216 44 649.0 0 44 217 40 649.0 0 40 218 49 649.0 0 49 219 61 649.0 0 61 220 91 649.0 0 91 221 78 649.0 0 78 222 108 649.0 0 108 223 65 649.0 0 65 224 79 649.0 0 79 225 79 649.0 0 79 226 91 649.0 0 91 227 114 649.0 0 114 228 135 649.0 0 135 229 128 649.0 0 128 230 50 649.0 0 50 231 65 649.0 0 65 232 56 649.0 0 56 233 84 649.0 0 84 234 54 649.0 0 54 235 54 649.0 0 54 236 52 649.0 0 52 237 69 649.0 0 69 238 56 649.0 0 56 239 68 649.0 0 68 240 94 649.0 0 94 241 176 649.0 0 176 242 145 649.0 0 145 243 124 649.0 0 124 244 82 649.0 0 82 245 87 649.0 0 87 246 127 649.0 0 127 247 73 649.0 0 73 248 93 649.0 0 93 249 63 649.0 0 63 250 87 649.0 0 87 251 64 649.0 0 64 252 61 649.0 0 61 253 53 649.0 0 53 254 78 649.0 0 78 255 299 649.0 0 299 256 149 649.0 0 149 257 201 649.0 0 201 258 115 649.0 0 115 259 480 649.0 0 480 260 478 649.0 0 478 261 579 649.0 0 579 262 186 649.0 0 186 263 205 649.0 0 205 264 92 649.0 0 92 265 115 649.0 0 115 266 100 649.0 0 100 267 218 649.0 0 218 268 250 649.0 0 250 269 169 649.0 0 169 270 91 649.0 0 91 271 39 649.0 0 39 272 59 649.0 0 59 273 132 649.0 0 132 274 67 649.0 0 67 275 124 649.0 0 124 276 98 649.0 0 98 277 153 649.0 0 153 278 57 649.0 0 57 279 97 649.0 0 97 280 130 649.0 0 130 281 258 649.0 0 258 282 204 649.0 0 204 283 145 649.0 0 145 284 47 649.0 0 47 285 55 649.0 0 55 286 42 649.0 0 42 287 55 649.0 0 55 288 69 649.0 0 69 289 78 649.0 0 78 290 55 649.0 0 55 291 47 649.0 0 47 292 37 649.0 0 37 293 68 649.0 0 68 294 88 649.0 0 88 295 102 649.0 0 102 296 89 649.0 0 89 297 92 649.0 0 92 298 47 649.0 0 47 299 52 649.0 0 52 300 82 649.0 0 82 301 177 649.0 0 177 Finished in 18.20 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 404,831 Reads with adapters: 86,603 (21.4%) Reads that were too short: 160 (0.0%) Reads written (passing filters): 86,443 (21.4%) Total basepairs processed: 98,756,961 bp Total written (filtered): 23,618,679 bp (23.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 86603 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 4 5 18 60 0.0 3 0 0 48 8 4 19 1868 0.0 3 0 1738 90 33 7 20 83503 0.0 4 79421 3436 488 125 33 21 157 0.0 4 16 126 8 1 6 22 21 0.0 4 1 2 11 0 7 23 156 0.0 4 1 0 1 117 37 24 7 0.0 4 2 0 0 0 5 29 2 0.0 4 1 0 0 0 1 30 3 0.0 4 0 0 0 0 3 32 1 0.0 4 1 35 2 0.0 4 0 0 0 0 2 36 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 45 2 0.0 4 1 0 0 0 1 48 24 0.0 4 0 0 0 0 24 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 39 0.0 4 0 0 0 1 38 78 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 3 0.0 4 0 0 0 0 3 98 1 0.0 4 0 0 0 1 110 3 0.0 4 0 0 0 0 3 119 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 555 0.0 4 0 0 0 1 554 128 7 0.0 4 0 0 0 0 7 129 3 0.0 4 0 0 0 0 3 130 7 0.0 4 0 0 0 1 6 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 1 0 0 1 144 2 0.0 4 0 0 0 0 2 145 2 0.0 4 0 0 0 0 2 146 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 149 17 0.0 4 0 0 0 2 15 150 3 0.0 4 0 0 0 0 3 151 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 8 0.0 4 0 0 0 0 8 154 6 0.0 4 0 0 0 0 6 155 10 0.0 4 0 0 0 0 10 156 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 3 0.0 4 0 0 0 0 3 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 3 0.0 4 0 0 0 0 3 211 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 213 1 0.0 4 1 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 0 2 219 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 18 0.0 4 0 0 0 0 18 229 11 0.0 4 0 0 0 1 10 230 2 0.0 4 0 0 0 1 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 1 248 1 0.0 4 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.97 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 86,443 Reads with adapters: 3,715 (4.3%) Reads written (passing filters): 86,443 (100.0%) Total basepairs processed: 23,618,679 bp Total written (filtered): 23,550,730 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3715 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.0% C: 13.5% G: 71.4% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 362 1350.7 0 362 4 42 337.7 0 42 5 4 84.4 0 4 6 1 21.1 0 1 7 1 5.3 0 1 9 17 0.3 0 2 15 10 67 0.1 1 2 65 11 2376 0.0 1 108 2268 12 552 0.0 1 13 539 13 73 0.0 1 0 73 14 5 0.0 1 0 5 15 7 0.0 1 0 7 16 2 0.0 1 0 2 20 27 0.0 1 19 8 21 8 0.0 1 7 1 22 1 0.0 1 1 23 1 0.0 1 0 1 24 10 0.0 1 9 1 41 1 0.0 1 1 105 1 0.0 1 1 106 1 0.0 1 0 1 108 1 0.0 1 0 1 115 2 0.0 1 2 124 1 0.0 1 1 150 1 0.0 1 0 1 152 21 0.0 1 13 8 153 3 0.0 1 3 158 6 0.0 1 5 1 159 1 0.0 1 1 171 2 0.0 1 2 180 2 0.0 1 2 183 1 0.0 1 0 1 191 3 0.0 1 0 3 196 1 0.0 1 1 208 2 0.0 1 1 1 210 92 0.0 1 73 19 211 8 0.0 1 5 3 221 4 0.0 1 4 256 1 0.0 1 1 271 1 0.0 1 1 273 3 0.0 1 3