This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.30 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 403,886 (15.2%) Reads that were too short: 30,175 (1.1%) Reads written (passing filters): 373,711 (14.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 92,475,108 bp (11.6%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 403886 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3421 649.0 0 3421 7 94801 649.0 0 94801 8 1666 649.0 0 1666 9 86848 649.0 0 86848 10 125 649.0 0 125 11 77 649.0 0 77 12 64 649.0 0 64 13 69 649.0 0 69 14 60 649.0 0 60 15 75 649.0 0 75 16 96 649.0 0 96 17 60 649.0 0 60 18 98 649.0 0 98 19 64 649.0 0 64 20 70 649.0 0 70 21 88 649.0 0 88 22 56 649.0 0 56 23 53 649.0 0 53 24 86 649.0 0 86 25 71 649.0 0 71 26 49 649.0 0 49 27 58 649.0 0 58 28 59 649.0 0 59 29 52 649.0 0 52 30 71 649.0 0 71 31 61 649.0 0 61 32 51 649.0 0 51 33 51 649.0 0 51 34 61 649.0 0 61 35 89 649.0 0 89 36 56 649.0 0 56 37 53 649.0 0 53 38 51 649.0 0 51 39 58 649.0 0 58 40 88 649.0 0 88 41 45 649.0 0 45 42 84 649.0 0 84 43 94 649.0 0 94 44 86 649.0 0 86 45 228 649.0 0 228 46 68 649.0 0 68 47 64 649.0 0 64 48 109 649.0 0 109 49 79 649.0 0 79 50 52 649.0 0 52 51 94 649.0 0 94 52 57 649.0 0 57 53 121 649.0 0 121 54 126 649.0 0 126 55 106 649.0 0 106 56 147 649.0 0 147 57 116 649.0 0 116 58 64 649.0 0 64 59 73 649.0 0 73 60 94 649.0 0 94 61 63 649.0 0 63 62 123 649.0 0 123 63 93 649.0 0 93 64 73 649.0 0 73 65 78 649.0 0 78 66 91 649.0 0 91 67 120 649.0 0 120 68 84 649.0 0 84 69 172 649.0 0 172 70 245 649.0 0 245 71 229 649.0 0 229 72 438 649.0 0 438 73 944 649.0 0 944 74 299 649.0 0 299 75 351 649.0 0 351 76 555 649.0 0 555 77 309 649.0 0 309 78 311 649.0 0 311 79 680 649.0 0 680 80 923 649.0 0 923 81 1065 649.0 0 1065 82 841 649.0 0 841 83 1344 649.0 0 1344 84 1337 649.0 0 1337 85 2313 649.0 0 2313 86 53164 649.0 0 53164 87 31284 649.0 0 31284 88 49835 649.0 0 49835 89 217 649.0 0 217 90 49 649.0 0 49 91 41 649.0 0 41 92 46 649.0 0 46 93 65 649.0 0 65 94 51 649.0 0 51 95 57 649.0 0 57 96 58 649.0 0 58 97 54 649.0 0 54 98 62 649.0 0 62 99 63 649.0 0 63 100 85 649.0 0 85 101 67 649.0 0 67 102 80 649.0 0 80 103 113 649.0 0 113 104 78 649.0 0 78 105 72 649.0 0 72 106 75 649.0 0 75 107 46 649.0 0 46 108 42 649.0 0 42 109 48 649.0 0 48 110 48 649.0 0 48 111 49 649.0 0 49 112 44 649.0 0 44 113 47 649.0 0 47 114 53 649.0 0 53 115 65 649.0 0 65 116 51 649.0 0 51 117 73 649.0 0 73 118 64 649.0 0 64 119 59 649.0 0 59 120 72 649.0 0 72 121 59 649.0 0 59 122 77 649.0 0 77 123 265 649.0 0 265 124 792 649.0 0 792 125 471 649.0 0 471 126 325 649.0 0 325 127 325 649.0 0 325 128 63 649.0 0 63 129 97 649.0 0 97 130 87 649.0 0 87 131 162 649.0 0 162 132 127 649.0 0 127 133 126 649.0 0 126 134 97 649.0 0 97 135 80 649.0 0 80 136 75 649.0 0 75 137 111 649.0 0 111 138 110 649.0 0 110 139 119 649.0 0 119 140 133 649.0 0 133 141 170 649.0 0 170 142 188 649.0 0 188 143 310 649.0 0 310 144 4540 649.0 0 4540 145 1942 649.0 0 1942 146 3690 649.0 0 3690 147 399 649.0 0 399 148 80 649.0 0 80 149 90 649.0 0 90 150 106 649.0 0 106 151 87 649.0 0 87 152 50 649.0 0 50 153 79 649.0 0 79 154 85 649.0 0 85 155 63 649.0 0 63 156 60 649.0 0 60 157 73 649.0 0 73 158 70 649.0 0 70 159 64 649.0 0 64 160 77 649.0 0 77 161 67 649.0 0 67 162 67 649.0 0 67 163 92 649.0 0 92 164 108 649.0 0 108 165 145 649.0 0 145 166 835 649.0 0 835 167 1251 649.0 0 1251 168 605 649.0 0 605 169 98 649.0 0 98 170 119 649.0 0 119 171 141 649.0 0 141 172 185 649.0 0 185 173 85 649.0 0 85 174 88 649.0 0 88 175 115 649.0 0 115 176 108 649.0 0 108 177 75 649.0 0 75 178 122 649.0 0 122 179 84 649.0 0 84 180 143 649.0 0 143 181 132 649.0 0 132 182 159 649.0 0 159 183 188 649.0 0 188 184 258 649.0 0 258 185 5308 649.0 0 5308 186 1530 649.0 0 1530 187 3746 649.0 0 3746 188 97 649.0 0 97 189 71 649.0 0 71 190 69 649.0 0 69 191 81 649.0 0 81 192 109 649.0 0 109 193 188 649.0 0 188 194 101 649.0 0 101 195 150 649.0 0 150 196 136 649.0 0 136 197 108 649.0 0 108 198 110 649.0 0 110 199 152 649.0 0 152 200 179 649.0 0 179 201 161 649.0 0 161 202 193 649.0 0 193 203 251 649.0 0 251 204 294 649.0 0 294 205 457 649.0 0 457 206 14265 649.0 0 14265 207 2557 649.0 0 2557 208 4367 649.0 0 4367 209 71 649.0 0 71 210 77 649.0 0 77 211 291 649.0 0 291 212 304 649.0 0 304 213 376 649.0 0 376 214 73 649.0 0 73 215 59 649.0 0 59 216 60 649.0 0 60 217 41 649.0 0 41 218 46 649.0 0 46 219 49 649.0 0 49 220 50 649.0 0 50 221 56 649.0 0 56 222 85 649.0 0 85 223 46 649.0 0 46 224 52 649.0 0 52 225 68 649.0 0 68 226 76 649.0 0 76 227 79 649.0 0 79 228 101 649.0 0 101 229 80 649.0 0 80 230 56 649.0 0 56 231 67 649.0 0 67 232 64 649.0 0 64 233 58 649.0 0 58 234 71 649.0 0 71 235 64 649.0 0 64 236 49 649.0 0 49 237 43 649.0 0 43 238 37 649.0 0 37 239 60 649.0 0 60 240 68 649.0 0 68 241 90 649.0 0 90 242 67 649.0 0 67 243 60 649.0 0 60 244 53 649.0 0 53 245 53 649.0 0 53 246 58 649.0 0 58 247 60 649.0 0 60 248 74 649.0 0 74 249 58 649.0 0 58 250 90 649.0 0 90 251 58 649.0 0 58 252 61 649.0 0 61 253 51 649.0 0 51 254 48 649.0 0 48 255 171 649.0 0 171 256 84 649.0 0 84 257 118 649.0 0 118 258 112 649.0 0 112 259 182 649.0 0 182 260 351 649.0 0 351 261 165 649.0 0 165 262 70 649.0 0 70 263 98 649.0 0 98 264 60 649.0 0 60 265 76 649.0 0 76 266 63 649.0 0 63 267 122 649.0 0 122 268 109 649.0 0 109 269 112 649.0 0 112 270 67 649.0 0 67 271 34 649.0 0 34 272 61 649.0 0 61 273 150 649.0 0 150 274 35 649.0 0 35 275 69 649.0 0 69 276 55 649.0 0 55 277 101 649.0 0 101 278 47 649.0 0 47 279 80 649.0 0 80 280 83 649.0 0 83 281 119 649.0 0 119 282 128 649.0 0 128 283 78 649.0 0 78 284 47 649.0 0 47 285 40 649.0 0 40 286 50 649.0 0 50 287 37 649.0 0 37 288 68 649.0 0 68 289 60 649.0 0 60 290 49 649.0 0 49 291 60 649.0 0 60 292 54 649.0 0 54 293 62 649.0 0 62 294 80 649.0 0 80 295 95 649.0 0 95 296 64 649.0 0 64 297 97 649.0 0 97 298 37 649.0 0 37 299 58 649.0 0 58 300 73 649.0 0 73 301 102 649.0 0 102 Finished in 15.12 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 373,711 Reads with adapters: 90,453 (24.2%) Reads that were too short: 113 (0.0%) Reads written (passing filters): 90,340 (24.2%) Total basepairs processed: 92,475,108 bp Total written (filtered): 24,631,985 bp (26.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 90453 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 18 0.0 3 0 0 18 16 517 0.0 3 0 500 16 1 17 88158 0.0 3 86461 1642 49 6 18 206 0.0 3 15 183 6 2 19 45 0.0 3 2 1 38 4 20 78 0.0 3 1 0 1 76 21 7 0.0 3 0 0 0 7 22 3 0.0 3 1 0 0 2 26 1 0.0 3 1 37 1 0.0 3 0 0 0 1 38 16 0.0 3 0 0 0 16 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 62 1 0.0 3 0 1 63 1 0.0 3 0 0 0 1 66 7 0.0 3 0 0 0 7 67 1 0.0 3 0 0 1 69 2 0.0 3 0 0 0 2 80 35 0.0 3 0 0 0 35 82 1 0.0 3 0 0 0 1 83 11 0.0 3 0 0 0 11 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 90 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 102 3 0.0 3 0 0 0 3 103 2 0.0 3 0 0 0 2 104 172 0.0 3 0 0 0 172 105 879 0.0 3 0 0 3 876 106 43 0.0 3 0 0 0 43 108 88 0.0 3 0 0 1 87 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 115 1 0.0 3 1 118 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 7 0.0 3 0 0 0 7 158 4 0.0 3 0 0 0 4 164 6 0.0 3 0 0 0 6 165 5 0.0 3 0 0 0 5 166 2 0.0 3 0 0 0 2 167 3 0.0 3 1 0 0 2 177 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 5 0.0 3 0 0 0 5 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 1 1 204 1 0.0 3 0 0 0 1 206 31 0.0 3 0 0 0 31 207 19 0.0 3 0 0 0 19 217 4 0.0 3 0 0 0 4 220 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 245 3 0.0 3 0 0 1 2 255 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.20 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 90,340 Reads with adapters: 3,304 (3.7%) Reads written (passing filters): 90,340 (100.0%) Total basepairs processed: 24,631,985 bp Total written (filtered): 24,621,969 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3304 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 82.6% C: 7.7% G: 8.2% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3222 1411.6 0 3222 4 74 352.9 0 74 5 5 88.2 0 5 6 1 22.1 0 1 11 1 0.0 1 0 1 12 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.