This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.81 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 137,691 (5.2%) Reads that were too short: 40,005 (1.5%) Reads written (passing filters): 97,686 (3.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 21,875,974 bp (2.7%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 137691 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 748 649.0 0 748 7 916 649.0 0 916 8 18933 649.0 0 18933 9 15939 649.0 0 15939 10 115 649.0 0 115 11 77 649.0 0 77 12 70 649.0 0 70 13 55 649.0 0 55 14 70 649.0 0 70 15 94 649.0 0 94 16 94 649.0 0 94 17 97 649.0 0 97 18 86 649.0 0 86 19 116 649.0 0 116 20 87 649.0 0 87 21 73 649.0 0 73 22 61 649.0 0 61 23 72 649.0 0 72 24 105 649.0 0 105 25 84 649.0 0 84 26 97 649.0 0 97 27 97 649.0 0 97 28 106 649.0 0 106 29 73 649.0 0 73 30 91 649.0 0 91 31 110 649.0 0 110 32 147 649.0 0 147 33 131 649.0 0 131 34 101 649.0 0 101 35 161 649.0 0 161 36 143 649.0 0 143 37 1337 649.0 0 1337 38 1136 649.0 0 1136 39 1815 649.0 0 1815 40 277 649.0 0 277 41 115 649.0 0 115 42 106 649.0 0 106 43 135 649.0 0 135 44 128 649.0 0 128 45 114 649.0 0 114 46 129 649.0 0 129 47 91 649.0 0 91 48 90 649.0 0 90 49 96 649.0 0 96 50 70 649.0 0 70 51 84 649.0 0 84 52 95 649.0 0 95 53 74 649.0 0 74 54 88 649.0 0 88 55 122 649.0 0 122 56 101 649.0 0 101 57 100 649.0 0 100 58 91 649.0 0 91 59 79 649.0 0 79 60 95 649.0 0 95 61 90 649.0 0 90 62 83 649.0 0 83 63 114 649.0 0 114 64 117 649.0 0 117 65 88 649.0 0 88 66 108 649.0 0 108 67 103 649.0 0 103 68 83 649.0 0 83 69 122 649.0 0 122 70 112 649.0 0 112 71 127 649.0 0 127 72 128 649.0 0 128 73 143 649.0 0 143 74 125 649.0 0 125 75 163 649.0 0 163 76 288 649.0 0 288 77 238 649.0 0 238 78 1096 649.0 0 1096 79 307 649.0 0 307 80 129 649.0 0 129 81 92 649.0 0 92 82 140 649.0 0 140 83 100 649.0 0 100 84 113 649.0 0 113 85 90 649.0 0 90 86 114 649.0 0 114 87 82 649.0 0 82 88 219 649.0 0 219 89 229 649.0 0 229 90 219 649.0 0 219 91 362 649.0 0 362 92 887 649.0 0 887 93 722 649.0 0 722 94 479 649.0 0 479 95 343 649.0 0 343 96 87 649.0 0 87 97 117 649.0 0 117 98 88 649.0 0 88 99 103 649.0 0 103 100 99 649.0 0 99 101 83 649.0 0 83 102 80 649.0 0 80 103 168 649.0 0 168 104 310 649.0 0 310 105 107 649.0 0 107 106 278 649.0 0 278 107 123 649.0 0 123 108 105 649.0 0 105 109 134 649.0 0 134 110 163 649.0 0 163 111 112 649.0 0 112 112 232 649.0 0 232 113 187 649.0 0 187 114 246 649.0 0 246 115 205 649.0 0 205 116 207 649.0 0 207 117 137 649.0 0 137 118 129 649.0 0 129 119 107 649.0 0 107 120 112 649.0 0 112 121 117 649.0 0 117 122 129 649.0 0 129 123 124 649.0 0 124 124 157 649.0 0 157 125 109 649.0 0 109 126 143 649.0 0 143 127 191 649.0 0 191 128 310 649.0 0 310 129 2799 649.0 0 2799 130 1124 649.0 0 1124 131 852 649.0 0 852 132 215 649.0 0 215 133 160 649.0 0 160 134 161 649.0 0 161 135 215 649.0 0 215 136 237 649.0 0 237 137 258 649.0 0 258 138 226 649.0 0 226 139 452 649.0 0 452 140 359 649.0 0 359 141 945 649.0 0 945 142 11797 649.0 0 11797 143 4536 649.0 0 4536 144 8847 649.0 0 8847 145 148 649.0 0 148 146 103 649.0 0 103 147 90 649.0 0 90 148 102 649.0 0 102 149 210 649.0 0 210 150 176 649.0 0 176 151 196 649.0 0 196 152 85 649.0 0 85 153 121 649.0 0 121 154 123 649.0 0 123 155 100 649.0 0 100 156 112 649.0 0 112 157 101 649.0 0 101 158 95 649.0 0 95 159 86 649.0 0 86 160 79 649.0 0 79 161 80 649.0 0 80 162 79 649.0 0 79 163 94 649.0 0 94 164 91 649.0 0 91 165 88 649.0 0 88 166 81 649.0 0 81 167 90 649.0 0 90 168 93 649.0 0 93 169 109 649.0 0 109 170 80 649.0 0 80 171 74 649.0 0 74 172 60 649.0 0 60 173 86 649.0 0 86 174 90 649.0 0 90 175 93 649.0 0 93 176 83 649.0 0 83 177 123 649.0 0 123 178 201 649.0 0 201 179 236 649.0 0 236 180 76 649.0 0 76 181 75 649.0 0 75 182 83 649.0 0 83 183 105 649.0 0 105 184 71 649.0 0 71 185 88 649.0 0 88 186 112 649.0 0 112 187 91 649.0 0 91 188 95 649.0 0 95 189 91 649.0 0 91 190 135 649.0 0 135 191 100 649.0 0 100 192 106 649.0 0 106 193 80 649.0 0 80 194 69 649.0 0 69 195 70 649.0 0 70 196 86 649.0 0 86 197 95 649.0 0 95 198 84 649.0 0 84 199 85 649.0 0 85 200 71 649.0 0 71 201 106 649.0 0 106 202 150 649.0 0 150 203 164 649.0 0 164 204 122 649.0 0 122 205 111 649.0 0 111 206 97 649.0 0 97 207 242 649.0 0 242 208 194 649.0 0 194 209 1609 649.0 0 1609 210 447 649.0 0 447 211 899 649.0 0 899 212 160 649.0 0 160 213 235 649.0 0 235 214 1197 649.0 0 1197 215 903 649.0 0 903 216 1712 649.0 0 1712 217 196 649.0 0 196 218 402 649.0 0 402 219 120 649.0 0 120 220 203 649.0 0 203 221 156 649.0 0 156 222 186 649.0 0 186 223 148 649.0 0 148 224 159 649.0 0 159 225 143 649.0 0 143 226 257 649.0 0 257 227 253 649.0 0 253 228 389 649.0 0 389 229 1685 649.0 0 1685 230 654 649.0 0 654 231 1226 649.0 0 1226 232 375 649.0 0 375 233 495 649.0 0 495 234 4385 649.0 0 4385 235 3875 649.0 0 3875 236 5069 649.0 0 5069 237 2324 649.0 0 2324 238 107 649.0 0 107 239 69 649.0 0 69 240 136 649.0 0 136 241 100 649.0 0 100 242 69 649.0 0 69 243 88 649.0 0 88 244 73 649.0 0 73 245 61 649.0 0 61 246 51 649.0 0 51 247 94 649.0 0 94 248 76 649.0 0 76 249 49 649.0 0 49 250 80 649.0 0 80 251 65 649.0 0 65 252 60 649.0 0 60 253 134 649.0 0 134 254 189 649.0 0 189 255 159 649.0 0 159 256 119 649.0 0 119 257 66 649.0 0 66 258 70 649.0 0 70 259 73 649.0 0 73 260 91 649.0 0 91 261 109 649.0 0 109 262 305 649.0 0 305 263 159 649.0 0 159 264 237 649.0 0 237 265 140 649.0 0 140 266 127 649.0 0 127 267 60 649.0 0 60 268 59 649.0 0 59 269 83 649.0 0 83 270 140 649.0 0 140 271 141 649.0 0 141 272 169 649.0 0 169 273 80 649.0 0 80 274 75 649.0 0 75 275 88 649.0 0 88 276 108 649.0 0 108 277 81 649.0 0 81 278 105 649.0 0 105 279 82 649.0 0 82 280 73 649.0 0 73 281 101 649.0 0 101 282 86 649.0 0 86 283 87 649.0 0 87 284 125 649.0 0 125 285 81 649.0 0 81 286 82 649.0 0 82 287 79 649.0 0 79 288 64 649.0 0 64 289 62 649.0 0 62 290 58 649.0 0 58 291 146 649.0 0 146 292 87 649.0 0 87 293 127 649.0 0 127 294 105 649.0 0 105 295 346 649.0 0 346 296 774 649.0 0 774 297 854 649.0 0 854 298 709 649.0 0 709 299 389 649.0 0 389 300 89 649.0 0 89 301 112 649.0 0 112 Finished in 4.53 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 97,686 Reads with adapters: 17,402 (17.8%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 17,367 (17.8%) Total basepairs processed: 21,875,974 bp Total written (filtered): 4,653,701 bp (21.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17402 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 14 0.0 3 0 0 8 2 4 19 136 0.0 3 0 114 7 9 6 20 16398 0.0 4 15496 733 130 30 9 21 51 0.0 4 13 35 3 22 3 0.0 4 0 0 3 23 93 0.0 4 31 0 0 48 14 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 26 2 0.0 4 0 2 27 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 1 40 1 0.0 4 0 0 0 0 1 42 117 0.0 4 0 0 0 0 117 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 49 2 0.0 4 0 0 0 0 2 58 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 69 29 0.0 4 0 0 0 0 29 79 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 97 62 0.0 4 0 0 0 0 62 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 1 119 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 431 0.0 4 0 0 0 0 431 128 2 0.0 4 0 0 0 0 2 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 1 138 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 188 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.63 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 17,367 Reads with adapters: 3,849 (22.2%) Reads written (passing filters): 17,367 (100.0%) Total basepairs processed: 4,653,701 bp Total written (filtered): 4,612,123 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3849 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 8.0% G: 87.2% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 271.4 0 5 4 2 67.8 0 2 6 1 4.2 0 1 9 697 0.1 0 69 628 10 2360 0.0 1 146 2214 11 745 0.0 1 33 712 12 13 0.0 1 0 13 13 6 0.0 1 0 6 14 2 0.0 1 0 2 72 1 0.0 1 1 80 1 0.0 1 1 150 3 0.0 1 3 152 1 0.0 1 1 153 1 0.0 1 1 178 1 0.0 1 1 208 8 0.0 1 6 2 211 1 0.0 1 0 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.