This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.10 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 113,505 (4.3%) Reads that were too short: 21,116 (0.8%) Reads written (passing filters): 92,389 (3.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 20,874,228 bp (2.6%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 113505 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 708 649.0 0 708 7 759 649.0 0 759 8 17825 649.0 0 17825 9 18390 649.0 0 18390 10 76 649.0 0 76 11 68 649.0 0 68 12 45 649.0 0 45 13 72 649.0 0 72 14 60 649.0 0 60 15 62 649.0 0 62 16 68 649.0 0 68 17 72 649.0 0 72 18 59 649.0 0 59 19 77 649.0 0 77 20 56 649.0 0 56 21 76 649.0 0 76 22 62 649.0 0 62 23 77 649.0 0 77 24 62 649.0 0 62 25 57 649.0 0 57 26 78 649.0 0 78 27 82 649.0 0 82 28 72 649.0 0 72 29 50 649.0 0 50 30 69 649.0 0 69 31 72 649.0 0 72 32 101 649.0 0 101 33 90 649.0 0 90 34 97 649.0 0 97 35 101 649.0 0 101 36 109 649.0 0 109 37 840 649.0 0 840 38 986 649.0 0 986 39 1464 649.0 0 1464 40 85 649.0 0 85 41 65 649.0 0 65 42 89 649.0 0 89 43 114 649.0 0 114 44 109 649.0 0 109 45 91 649.0 0 91 46 92 649.0 0 92 47 100 649.0 0 100 48 61 649.0 0 61 49 102 649.0 0 102 50 77 649.0 0 77 51 92 649.0 0 92 52 79 649.0 0 79 53 73 649.0 0 73 54 75 649.0 0 75 55 76 649.0 0 76 56 90 649.0 0 90 57 66 649.0 0 66 58 80 649.0 0 80 59 81 649.0 0 81 60 72 649.0 0 72 61 60 649.0 0 60 62 59 649.0 0 59 63 73 649.0 0 73 64 82 649.0 0 82 65 102 649.0 0 102 66 65 649.0 0 65 67 81 649.0 0 81 68 90 649.0 0 90 69 78 649.0 0 78 70 98 649.0 0 98 71 95 649.0 0 95 72 75 649.0 0 75 73 118 649.0 0 118 74 82 649.0 0 82 75 136 649.0 0 136 76 284 649.0 0 284 77 172 649.0 0 172 78 1183 649.0 0 1183 79 273 649.0 0 273 80 137 649.0 0 137 81 68 649.0 0 68 82 79 649.0 0 79 83 67 649.0 0 67 84 56 649.0 0 56 85 78 649.0 0 78 86 52 649.0 0 52 87 52 649.0 0 52 88 151 649.0 0 151 89 202 649.0 0 202 90 128 649.0 0 128 91 270 649.0 0 270 92 423 649.0 0 423 93 568 649.0 0 568 94 310 649.0 0 310 95 263 649.0 0 263 96 79 649.0 0 79 97 84 649.0 0 84 98 62 649.0 0 62 99 65 649.0 0 65 100 67 649.0 0 67 101 69 649.0 0 69 102 72 649.0 0 72 103 124 649.0 0 124 104 337 649.0 0 337 105 79 649.0 0 79 106 296 649.0 0 296 107 90 649.0 0 90 108 73 649.0 0 73 109 98 649.0 0 98 110 148 649.0 0 148 111 117 649.0 0 117 112 178 649.0 0 178 113 140 649.0 0 140 114 197 649.0 0 197 115 169 649.0 0 169 116 179 649.0 0 179 117 93 649.0 0 93 118 86 649.0 0 86 119 76 649.0 0 76 120 86 649.0 0 86 121 72 649.0 0 72 122 112 649.0 0 112 123 88 649.0 0 88 124 107 649.0 0 107 125 93 649.0 0 93 126 106 649.0 0 106 127 138 649.0 0 138 128 247 649.0 0 247 129 3051 649.0 0 3051 130 1144 649.0 0 1144 131 985 649.0 0 985 132 163 649.0 0 163 133 110 649.0 0 110 134 124 649.0 0 124 135 146 649.0 0 146 136 208 649.0 0 208 137 218 649.0 0 218 138 186 649.0 0 186 139 343 649.0 0 343 140 289 649.0 0 289 141 825 649.0 0 825 142 11538 649.0 0 11538 143 4435 649.0 0 4435 144 9609 649.0 0 9609 145 129 649.0 0 129 146 66 649.0 0 66 147 55 649.0 0 55 148 78 649.0 0 78 149 126 649.0 0 126 150 121 649.0 0 121 151 108 649.0 0 108 152 66 649.0 0 66 153 81 649.0 0 81 154 77 649.0 0 77 155 73 649.0 0 73 156 79 649.0 0 79 157 101 649.0 0 101 158 53 649.0 0 53 159 47 649.0 0 47 160 58 649.0 0 58 161 59 649.0 0 59 162 40 649.0 0 40 163 70 649.0 0 70 164 81 649.0 0 81 165 52 649.0 0 52 166 64 649.0 0 64 167 47 649.0 0 47 168 60 649.0 0 60 169 98 649.0 0 98 170 76 649.0 0 76 171 44 649.0 0 44 172 52 649.0 0 52 173 65 649.0 0 65 174 74 649.0 0 74 175 64 649.0 0 64 176 70 649.0 0 70 177 72 649.0 0 72 178 153 649.0 0 153 179 220 649.0 0 220 180 88 649.0 0 88 181 52 649.0 0 52 182 71 649.0 0 71 183 85 649.0 0 85 184 37 649.0 0 37 185 39 649.0 0 39 186 71 649.0 0 71 187 52 649.0 0 52 188 64 649.0 0 64 189 62 649.0 0 62 190 108 649.0 0 108 191 74 649.0 0 74 192 63 649.0 0 63 193 57 649.0 0 57 194 55 649.0 0 55 195 53 649.0 0 53 196 69 649.0 0 69 197 74 649.0 0 74 198 53 649.0 0 53 199 53 649.0 0 53 200 46 649.0 0 46 201 62 649.0 0 62 202 108 649.0 0 108 203 95 649.0 0 95 204 73 649.0 0 73 205 80 649.0 0 80 206 55 649.0 0 55 207 130 649.0 0 130 208 95 649.0 0 95 209 652 649.0 0 652 210 154 649.0 0 154 211 353 649.0 0 353 212 82 649.0 0 82 213 113 649.0 0 113 214 460 649.0 0 460 215 393 649.0 0 393 216 574 649.0 0 574 217 103 649.0 0 103 218 197 649.0 0 197 219 72 649.0 0 72 220 105 649.0 0 105 221 89 649.0 0 89 222 122 649.0 0 122 223 83 649.0 0 83 224 122 649.0 0 122 225 98 649.0 0 98 226 130 649.0 0 130 227 130 649.0 0 130 228 179 649.0 0 179 229 996 649.0 0 996 230 332 649.0 0 332 231 709 649.0 0 709 232 169 649.0 0 169 233 300 649.0 0 300 234 1839 649.0 0 1839 235 1989 649.0 0 1989 236 2443 649.0 0 2443 237 1442 649.0 0 1442 238 81 649.0 0 81 239 60 649.0 0 60 240 137 649.0 0 137 241 45 649.0 0 45 242 31 649.0 0 31 243 52 649.0 0 52 244 63 649.0 0 63 245 66 649.0 0 66 246 61 649.0 0 61 247 60 649.0 0 60 248 56 649.0 0 56 249 54 649.0 0 54 250 43 649.0 0 43 251 52 649.0 0 52 252 47 649.0 0 47 253 80 649.0 0 80 254 103 649.0 0 103 255 59 649.0 0 59 256 71 649.0 0 71 257 63 649.0 0 63 258 54 649.0 0 54 259 65 649.0 0 65 260 67 649.0 0 67 261 79 649.0 0 79 262 341 649.0 0 341 263 123 649.0 0 123 264 204 649.0 0 204 265 79 649.0 0 79 266 88 649.0 0 88 267 43 649.0 0 43 268 52 649.0 0 52 269 43 649.0 0 43 270 104 649.0 0 104 271 76 649.0 0 76 272 77 649.0 0 77 273 56 649.0 0 56 274 64 649.0 0 64 275 59 649.0 0 59 276 70 649.0 0 70 277 81 649.0 0 81 278 70 649.0 0 70 279 56 649.0 0 56 280 43 649.0 0 43 281 67 649.0 0 67 282 53 649.0 0 53 283 65 649.0 0 65 284 78 649.0 0 78 285 70 649.0 0 70 286 64 649.0 0 64 287 93 649.0 0 93 288 63 649.0 0 63 289 61 649.0 0 61 290 47 649.0 0 47 291 125 649.0 0 125 292 72 649.0 0 72 293 84 649.0 0 84 294 79 649.0 0 79 295 171 649.0 0 171 296 465 649.0 0 465 297 411 649.0 0 411 298 367 649.0 0 367 299 149 649.0 0 149 300 54 649.0 0 54 301 134 649.0 0 134 Finished in 3.85 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 92,389 Reads with adapters: 18,166 (19.7%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 18,132 (19.6%) Total basepairs processed: 20,874,228 bp Total written (filtered): 4,992,144 bp (23.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18166 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 107 0.0 3 0 103 3 1 17 17581 0.0 3 17209 359 13 18 45 0.0 3 11 34 19 24 0.0 3 1 5 13 5 20 266 0.0 3 234 6 3 23 21 1 0.0 3 0 1 22 1 0.0 3 0 0 0 1 32 1 0.0 3 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 1 43 62 0.0 3 0 0 1 61 46 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 6 0.0 3 0 0 0 6 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 75 1 0.0 3 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 1 94 1 0.0 3 0 0 1 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 104 2 0.0 3 1 0 0 1 113 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 161 1 0.0 3 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 1 175 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 244 6 0.0 3 0 0 0 6 262 3 0.0 3 0 0 0 3 266 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 18,132 Reads with adapters: 144 (0.8%) Reads written (passing filters): 18,132 (100.0%) Total basepairs processed: 4,992,144 bp Total written (filtered): 4,991,677 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 144 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.9% C: 6.2% G: 29.2% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 115 283.3 0 115 4 24 70.8 0 24 5 4 17.7 0 4 6 1 4.4 0 1