This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.35 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 280,749 (10.6%) Reads that were too short: 16,531 (0.6%) Reads written (passing filters): 264,218 (9.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 75,402,450 bp (9.4%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 280749 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2358 649.0 0 2358 7 136836 649.0 0 136836 8 105714 649.0 0 105714 9 112 649.0 0 112 10 56 649.0 0 56 11 40 649.0 0 40 12 35 649.0 0 35 13 52 649.0 0 52 14 36 649.0 0 36 15 28 649.0 0 28 16 40 649.0 0 40 17 22 649.0 0 22 18 45 649.0 0 45 19 29 649.0 0 29 20 35 649.0 0 35 21 41 649.0 0 41 22 56 649.0 0 56 23 22 649.0 0 22 24 31 649.0 0 31 25 24 649.0 0 24 26 25 649.0 0 25 27 45 649.0 0 45 28 35 649.0 0 35 29 252 649.0 0 252 30 41 649.0 0 41 31 290 649.0 0 290 32 42 649.0 0 42 33 37 649.0 0 37 34 37 649.0 0 37 35 45 649.0 0 45 36 38 649.0 0 38 37 44 649.0 0 44 38 33 649.0 0 33 39 34 649.0 0 34 40 40 649.0 0 40 41 40 649.0 0 40 42 17 649.0 0 17 43 25 649.0 0 25 44 32 649.0 0 32 45 40 649.0 0 40 46 43 649.0 0 43 47 35 649.0 0 35 48 42 649.0 0 42 49 180 649.0 0 180 50 34 649.0 0 34 51 27 649.0 0 27 52 66 649.0 0 66 53 29 649.0 0 29 54 58 649.0 0 58 55 132 649.0 0 132 56 216 649.0 0 216 57 37 649.0 0 37 58 40 649.0 0 40 59 56 649.0 0 56 60 59 649.0 0 59 61 626 649.0 0 626 62 196 649.0 0 196 63 563 649.0 0 563 64 41 649.0 0 41 65 38 649.0 0 38 66 27 649.0 0 27 67 98 649.0 0 98 68 129 649.0 0 129 69 91 649.0 0 91 70 37 649.0 0 37 71 59 649.0 0 59 72 47 649.0 0 47 73 380 649.0 0 380 74 112 649.0 0 112 75 69 649.0 0 69 76 68 649.0 0 68 77 29 649.0 0 29 78 69 649.0 0 69 79 83 649.0 0 83 80 78 649.0 0 78 81 72 649.0 0 72 82 89 649.0 0 89 83 115 649.0 0 115 84 95 649.0 0 95 85 167 649.0 0 167 86 206 649.0 0 206 87 151 649.0 0 151 88 193 649.0 0 193 89 88 649.0 0 88 90 52 649.0 0 52 91 45 649.0 0 45 92 61 649.0 0 61 93 32 649.0 0 32 94 76 649.0 0 76 95 60 649.0 0 60 96 55 649.0 0 55 97 76 649.0 0 76 98 68 649.0 0 68 99 49 649.0 0 49 100 43 649.0 0 43 101 44 649.0 0 44 102 28 649.0 0 28 103 29 649.0 0 29 104 23 649.0 0 23 105 25 649.0 0 25 106 24 649.0 0 24 107 19 649.0 0 19 108 33 649.0 0 33 109 33 649.0 0 33 110 30 649.0 0 30 111 43 649.0 0 43 112 92 649.0 0 92 113 72 649.0 0 72 114 75 649.0 0 75 115 49 649.0 0 49 116 47 649.0 0 47 117 44 649.0 0 44 118 61 649.0 0 61 119 65 649.0 0 65 120 68 649.0 0 68 121 76 649.0 0 76 122 58 649.0 0 58 123 134 649.0 0 134 124 135 649.0 0 135 125 256 649.0 0 256 126 208 649.0 0 208 127 241 649.0 0 241 128 605 649.0 0 605 129 477 649.0 0 477 130 473 649.0 0 473 131 109 649.0 0 109 132 68 649.0 0 68 133 90 649.0 0 90 134 282 649.0 0 282 135 128 649.0 0 128 136 195 649.0 0 195 137 72 649.0 0 72 138 66 649.0 0 66 139 71 649.0 0 71 140 79 649.0 0 79 141 65 649.0 0 65 142 50 649.0 0 50 143 73 649.0 0 73 144 70 649.0 0 70 145 95 649.0 0 95 146 110 649.0 0 110 147 79 649.0 0 79 148 78 649.0 0 78 149 72 649.0 0 72 150 97 649.0 0 97 151 71 649.0 0 71 152 85 649.0 0 85 153 59 649.0 0 59 154 51 649.0 0 51 155 64 649.0 0 64 156 91 649.0 0 91 157 39 649.0 0 39 158 141 649.0 0 141 159 54 649.0 0 54 160 52 649.0 0 52 161 51 649.0 0 51 162 59 649.0 0 59 163 51 649.0 0 51 164 60 649.0 0 60 165 86 649.0 0 86 166 305 649.0 0 305 167 133 649.0 0 133 168 132 649.0 0 132 169 44 649.0 0 44 170 44 649.0 0 44 171 57 649.0 0 57 172 54 649.0 0 54 173 59 649.0 0 59 174 100 649.0 0 100 175 74 649.0 0 74 176 97 649.0 0 97 177 86 649.0 0 86 178 71 649.0 0 71 179 51 649.0 0 51 180 59 649.0 0 59 181 45 649.0 0 45 182 65 649.0 0 65 183 121 649.0 0 121 184 73 649.0 0 73 185 107 649.0 0 107 186 78 649.0 0 78 187 77 649.0 0 77 188 104 649.0 0 104 189 64 649.0 0 64 190 70 649.0 0 70 191 88 649.0 0 88 192 85 649.0 0 85 193 103 649.0 0 103 194 116 649.0 0 116 195 172 649.0 0 172 196 267 649.0 0 267 197 524 649.0 0 524 198 895 649.0 0 895 199 606 649.0 0 606 200 101 649.0 0 101 201 70 649.0 0 70 202 49 649.0 0 49 203 43 649.0 0 43 204 60 649.0 0 60 205 81 649.0 0 81 206 78 649.0 0 78 207 87 649.0 0 87 208 104 649.0 0 104 209 91 649.0 0 91 210 119 649.0 0 119 211 191 649.0 0 191 212 111 649.0 0 111 213 174 649.0 0 174 214 93 649.0 0 93 215 87 649.0 0 87 216 71 649.0 0 71 217 88 649.0 0 88 218 165 649.0 0 165 219 222 649.0 0 222 220 585 649.0 0 585 221 356 649.0 0 356 222 851 649.0 0 851 223 125 649.0 0 125 224 447 649.0 0 447 225 105 649.0 0 105 226 63 649.0 0 63 227 66 649.0 0 66 228 72 649.0 0 72 229 69 649.0 0 69 230 105 649.0 0 105 231 126 649.0 0 126 232 150 649.0 0 150 233 122 649.0 0 122 234 90 649.0 0 90 235 98 649.0 0 98 236 81 649.0 0 81 237 82 649.0 0 82 238 172 649.0 0 172 239 151 649.0 0 151 240 351 649.0 0 351 241 203 649.0 0 203 242 241 649.0 0 241 243 144 649.0 0 144 244 173 649.0 0 173 245 157 649.0 0 157 246 180 649.0 0 180 247 194 649.0 0 194 248 145 649.0 0 145 249 166 649.0 0 166 250 126 649.0 0 126 251 121 649.0 0 121 252 89 649.0 0 89 253 85 649.0 0 85 254 144 649.0 0 144 255 140 649.0 0 140 256 165 649.0 0 165 257 89 649.0 0 89 258 85 649.0 0 85 259 88 649.0 0 88 260 89 649.0 0 89 261 106 649.0 0 106 262 230 649.0 0 230 263 220 649.0 0 220 264 407 649.0 0 407 265 104 649.0 0 104 266 134 649.0 0 134 267 556 649.0 0 556 268 553 649.0 0 553 269 718 649.0 0 718 270 429 649.0 0 429 271 70 649.0 0 70 272 105 649.0 0 105 273 221 649.0 0 221 274 449 649.0 0 449 275 272 649.0 0 272 276 313 649.0 0 313 277 151 649.0 0 151 278 75 649.0 0 75 279 87 649.0 0 87 280 110 649.0 0 110 281 159 649.0 0 159 282 146 649.0 0 146 283 134 649.0 0 134 284 62 649.0 0 62 285 63 649.0 0 63 286 72 649.0 0 72 287 101 649.0 0 101 288 151 649.0 0 151 289 156 649.0 0 156 290 146 649.0 0 146 291 82 649.0 0 82 292 67 649.0 0 67 293 87 649.0 0 87 294 116 649.0 0 116 295 121 649.0 0 121 296 126 649.0 0 126 297 93 649.0 0 93 298 75 649.0 0 75 299 85 649.0 0 85 300 94 649.0 0 94 301 80 649.0 0 80 Finished in 13.04 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 264,218 Reads with adapters: 112,939 (42.7%) Reads that were too short: 113 (0.0%) Reads written (passing filters): 112,826 (42.7%) Total basepairs processed: 75,402,450 bp Total written (filtered): 30,677,416 bp (40.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 112939 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 7 3 18 47 0.0 3 0 0 29 13 5 19 1218 0.0 3 0 1031 85 63 39 20 109658 0.0 4 103944 4745 738 177 54 21 273 0.0 4 27 222 14 4 6 22 31 0.0 4 5 1 18 5 2 23 357 0.0 4 1 0 1 258 97 24 4 0.0 4 3 0 0 1 25 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 33 4 0.0 4 1 0 0 0 3 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 1 61 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 73 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 101 4 0.0 4 0 0 0 0 4 102 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 119 2 0.0 4 0 0 0 0 2 124 2 0.0 4 0 1 0 0 1 125 6 0.0 4 0 0 0 0 6 126 19 0.0 4 0 0 0 0 19 127 1164 0.0 4 0 0 0 0 1164 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 14 0.0 4 0 0 0 0 14 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 8 0.0 4 0 0 0 0 8 158 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 6 0.0 4 0 0 0 0 6 208 1 0.0 4 0 0 0 0 1 209 3 0.0 4 0 0 0 0 3 219 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 1 227 1 0.0 4 0 0 0 0 1 228 26 0.0 4 0 0 0 1 25 229 14 0.0 4 0 0 0 0 14 230 2 0.0 4 0 0 0 0 2 231 3 0.0 4 0 0 0 0 3 233 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 236 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 259 3 0.0 4 0 0 0 0 3 263 1 0.0 4 0 0 0 0 1 264 4 0.0 4 0 0 0 0 4 265 2 0.0 4 0 0 0 0 2 274 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 1 287 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.03 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 112,826 Reads with adapters: 15,741 (14.0%) Reads written (passing filters): 112,826 (100.0%) Total basepairs processed: 30,677,416 bp Total written (filtered): 30,483,850 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 15741 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 9.8% G: 84.6% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 211 1762.9 0 211 4 30 440.7 0 30 5 1 110.2 0 1 8 2 1.7 0 2 9 67 0.4 0 5 62 10 2536 0.1 1 200 2336 11 12404 0.0 1 453 11951 12 313 0.0 1 5 308 13 18 0.0 1 0 18 14 19 0.0 1 0 19 15 5 0.0 1 0 5 123 1 0.0 1 1 135 1 0.0 1 0 1 139 1 0.0 1 1 140 1 0.0 1 1 149 1 0.0 1 1 151 30 0.0 1 25 5 152 2 0.0 1 1 1 153 3 0.0 1 2 1 170 2 0.0 1 2 196 1 0.0 1 1 197 1 0.0 1 1 209 64 0.0 1 45 19 210 4 0.0 1 3 1 211 2 0.0 1 2 224 21 0.0 1 21 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.