This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.36 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 279,558 (10.5%) Reads that were too short: 12,226 (0.5%) Reads written (passing filters): 267,332 (10.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 76,512,126 bp (9.6%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 279558 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1573 649.0 0 1573 7 117984 649.0 0 117984 8 131212 649.0 0 131212 9 164 649.0 0 164 10 34 649.0 0 34 11 34 649.0 0 34 12 26 649.0 0 26 13 24 649.0 0 24 14 26 649.0 0 26 15 32 649.0 0 32 16 29 649.0 0 29 17 36 649.0 0 36 18 35 649.0 0 35 19 27 649.0 0 27 20 35 649.0 0 35 21 37 649.0 0 37 22 31 649.0 0 31 23 22 649.0 0 22 24 17 649.0 0 17 25 19 649.0 0 19 26 23 649.0 0 23 27 26 649.0 0 26 28 22 649.0 0 22 29 31 649.0 0 31 30 21 649.0 0 21 31 29 649.0 0 29 32 35 649.0 0 35 33 15 649.0 0 15 34 43 649.0 0 43 35 39 649.0 0 39 36 47 649.0 0 47 37 33 649.0 0 33 38 40 649.0 0 40 39 37 649.0 0 37 40 40 649.0 0 40 41 29 649.0 0 29 42 28 649.0 0 28 43 20 649.0 0 20 44 44 649.0 0 44 45 40 649.0 0 40 46 47 649.0 0 47 47 23 649.0 0 23 48 33 649.0 0 33 49 181 649.0 0 181 50 27 649.0 0 27 51 33 649.0 0 33 52 70 649.0 0 70 53 31 649.0 0 31 54 46 649.0 0 46 55 126 649.0 0 126 56 213 649.0 0 213 57 23 649.0 0 23 58 33 649.0 0 33 59 63 649.0 0 63 60 54 649.0 0 54 61 718 649.0 0 718 62 251 649.0 0 251 63 677 649.0 0 677 64 51 649.0 0 51 65 38 649.0 0 38 66 43 649.0 0 43 67 73 649.0 0 73 68 104 649.0 0 104 69 83 649.0 0 83 70 21 649.0 0 21 71 43 649.0 0 43 72 36 649.0 0 36 73 37 649.0 0 37 74 35 649.0 0 35 75 25 649.0 0 25 76 44 649.0 0 44 77 18 649.0 0 18 78 60 649.0 0 60 79 51 649.0 0 51 80 62 649.0 0 62 81 50 649.0 0 50 82 34 649.0 0 34 83 44 649.0 0 44 84 42 649.0 0 42 85 46 649.0 0 46 86 61 649.0 0 61 87 107 649.0 0 107 88 74 649.0 0 74 89 63 649.0 0 63 90 57 649.0 0 57 91 45 649.0 0 45 92 46 649.0 0 46 93 50 649.0 0 50 94 57 649.0 0 57 95 66 649.0 0 66 96 76 649.0 0 76 97 71 649.0 0 71 98 96 649.0 0 96 99 84 649.0 0 84 100 60 649.0 0 60 101 52 649.0 0 52 102 51 649.0 0 51 103 47 649.0 0 47 104 31 649.0 0 31 105 21 649.0 0 21 106 40 649.0 0 40 107 30 649.0 0 30 108 41 649.0 0 41 109 56 649.0 0 56 110 35 649.0 0 35 111 46 649.0 0 46 112 60 649.0 0 60 113 46 649.0 0 46 114 61 649.0 0 61 115 66 649.0 0 66 116 39 649.0 0 39 117 40 649.0 0 40 118 59 649.0 0 59 119 51 649.0 0 51 120 56 649.0 0 56 121 58 649.0 0 58 122 59 649.0 0 59 123 122 649.0 0 122 124 105 649.0 0 105 125 192 649.0 0 192 126 172 649.0 0 172 127 212 649.0 0 212 128 442 649.0 0 442 129 256 649.0 0 256 130 255 649.0 0 255 131 59 649.0 0 59 132 55 649.0 0 55 133 60 649.0 0 60 134 274 649.0 0 274 135 124 649.0 0 124 136 215 649.0 0 215 137 58 649.0 0 58 138 49 649.0 0 49 139 61 649.0 0 61 140 50 649.0 0 50 141 58 649.0 0 58 142 37 649.0 0 37 143 59 649.0 0 59 144 49 649.0 0 49 145 87 649.0 0 87 146 89 649.0 0 89 147 54 649.0 0 54 148 80 649.0 0 80 149 59 649.0 0 59 150 65 649.0 0 65 151 62 649.0 0 62 152 76 649.0 0 76 153 45 649.0 0 45 154 33 649.0 0 33 155 43 649.0 0 43 156 83 649.0 0 83 157 50 649.0 0 50 158 132 649.0 0 132 159 49 649.0 0 49 160 45 649.0 0 45 161 50 649.0 0 50 162 72 649.0 0 72 163 55 649.0 0 55 164 49 649.0 0 49 165 70 649.0 0 70 166 173 649.0 0 173 167 80 649.0 0 80 168 131 649.0 0 131 169 47 649.0 0 47 170 43 649.0 0 43 171 45 649.0 0 45 172 56 649.0 0 56 173 50 649.0 0 50 174 81 649.0 0 81 175 53 649.0 0 53 176 76 649.0 0 76 177 95 649.0 0 95 178 60 649.0 0 60 179 59 649.0 0 59 180 44 649.0 0 44 181 61 649.0 0 61 182 63 649.0 0 63 183 114 649.0 0 114 184 68 649.0 0 68 185 89 649.0 0 89 186 82 649.0 0 82 187 79 649.0 0 79 188 62 649.0 0 62 189 60 649.0 0 60 190 61 649.0 0 61 191 70 649.0 0 70 192 56 649.0 0 56 193 105 649.0 0 105 194 94 649.0 0 94 195 156 649.0 0 156 196 246 649.0 0 246 197 598 649.0 0 598 198 1043 649.0 0 1043 199 855 649.0 0 855 200 122 649.0 0 122 201 57 649.0 0 57 202 55 649.0 0 55 203 46 649.0 0 46 204 60 649.0 0 60 205 60 649.0 0 60 206 59 649.0 0 59 207 63 649.0 0 63 208 67 649.0 0 67 209 79 649.0 0 79 210 82 649.0 0 82 211 94 649.0 0 94 212 99 649.0 0 99 213 130 649.0 0 130 214 105 649.0 0 105 215 82 649.0 0 82 216 70 649.0 0 70 217 71 649.0 0 71 218 119 649.0 0 119 219 163 649.0 0 163 220 355 649.0 0 355 221 302 649.0 0 302 222 714 649.0 0 714 223 108 649.0 0 108 224 510 649.0 0 510 225 94 649.0 0 94 226 74 649.0 0 74 227 49 649.0 0 49 228 68 649.0 0 68 229 66 649.0 0 66 230 95 649.0 0 95 231 88 649.0 0 88 232 100 649.0 0 100 233 80 649.0 0 80 234 74 649.0 0 74 235 79 649.0 0 79 236 100 649.0 0 100 237 71 649.0 0 71 238 174 649.0 0 174 239 153 649.0 0 153 240 301 649.0 0 301 241 135 649.0 0 135 242 155 649.0 0 155 243 115 649.0 0 115 244 117 649.0 0 117 245 90 649.0 0 90 246 113 649.0 0 113 247 113 649.0 0 113 248 125 649.0 0 125 249 93 649.0 0 93 250 83 649.0 0 83 251 80 649.0 0 80 252 67 649.0 0 67 253 63 649.0 0 63 254 119 649.0 0 119 255 131 649.0 0 131 256 180 649.0 0 180 257 78 649.0 0 78 258 79 649.0 0 79 259 69 649.0 0 69 260 75 649.0 0 75 261 81 649.0 0 81 262 204 649.0 0 204 263 300 649.0 0 300 264 670 649.0 0 670 265 61 649.0 0 61 266 92 649.0 0 92 267 225 649.0 0 225 268 207 649.0 0 207 269 254 649.0 0 254 270 191 649.0 0 191 271 58 649.0 0 58 272 64 649.0 0 64 273 120 649.0 0 120 274 256 649.0 0 256 275 142 649.0 0 142 276 113 649.0 0 113 277 82 649.0 0 82 278 60 649.0 0 60 279 81 649.0 0 81 280 67 649.0 0 67 281 120 649.0 0 120 282 79 649.0 0 79 283 106 649.0 0 106 284 55 649.0 0 55 285 37 649.0 0 37 286 64 649.0 0 64 287 83 649.0 0 83 288 94 649.0 0 94 289 102 649.0 0 102 290 84 649.0 0 84 291 60 649.0 0 60 292 59 649.0 0 59 293 64 649.0 0 64 294 71 649.0 0 71 295 112 649.0 0 112 296 70 649.0 0 70 297 74 649.0 0 74 298 66 649.0 0 66 299 66 649.0 0 66 300 100 649.0 0 100 301 93 649.0 0 93 Finished in 12.04 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 267,332 Reads with adapters: 114,017 (42.6%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 113,983 (42.6%) Total basepairs processed: 76,512,126 bp Total written (filtered): 31,565,660 bp (41.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 114017 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 16 0.0 3 0 0 16 16 751 0.0 3 0 732 16 3 17 112821 0.0 3 110594 2148 71 8 18 199 0.0 3 7 183 9 19 42 0.0 3 2 0 35 5 20 98 0.0 3 2 0 0 96 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 63 12 0.0 3 0 0 0 12 65 1 0.0 3 0 0 0 1 66 6 0.0 3 1 0 0 5 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 0 5 163 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 178 1 0.0 3 1 181 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 191 4 0.0 3 0 0 0 4 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 271 1 0.0 3 0 1 279 4 0.0 3 0 0 0 4 280 3 0.0 3 0 0 0 3 281 4 0.0 3 0 0 1 3 282 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.00 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 113,983 Reads with adapters: 1,704 (1.5%) Reads written (passing filters): 113,983 (100.0%) Total basepairs processed: 31,565,660 bp Total written (filtered): 31,560,387 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1704 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.4% C: 4.3% G: 37.6% T: 52.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1566 1781.0 0 1566 4 127 445.2 0 127 5 5 111.3 0 5 6 4 27.8 0 4 9 2 0.4 0 0 2