This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.06 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 62,938 (2.4%) Reads that were too short: 42,373 (1.6%) Reads written (passing filters): 20,565 (0.8%) Total basepairs processed: 800,111,879 bp Total written (filtered): 4,361,719 bp (0.5%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 62938 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 112 649.0 0 112 7 123 649.0 0 123 8 2506 649.0 0 2506 9 2276 649.0 0 2276 10 58 649.0 0 58 11 51 649.0 0 51 12 62 649.0 0 62 13 65 649.0 0 65 14 48 649.0 0 48 15 42 649.0 0 42 16 32 649.0 0 32 17 50 649.0 0 50 18 35 649.0 0 35 19 66 649.0 0 66 20 44 649.0 0 44 21 34 649.0 0 34 22 41 649.0 0 41 23 36 649.0 0 36 24 56 649.0 0 56 25 40 649.0 0 40 26 33 649.0 0 33 27 33 649.0 0 33 28 33 649.0 0 33 29 37 649.0 0 37 30 42 649.0 0 42 31 56 649.0 0 56 32 43 649.0 0 43 33 47 649.0 0 47 34 48 649.0 0 48 35 50 649.0 0 50 36 72 649.0 0 72 37 64 649.0 0 64 38 46 649.0 0 46 39 40 649.0 0 40 40 77 649.0 0 77 41 64 649.0 0 64 42 56 649.0 0 56 43 44 649.0 0 44 44 45 649.0 0 45 45 51 649.0 0 51 46 59 649.0 0 59 47 45 649.0 0 45 48 52 649.0 0 52 49 50 649.0 0 50 50 59 649.0 0 59 51 159 649.0 0 159 52 120 649.0 0 120 53 245 649.0 0 245 54 40 649.0 0 40 55 44 649.0 0 44 56 34 649.0 0 34 57 38 649.0 0 38 58 67 649.0 0 67 59 57 649.0 0 57 60 48 649.0 0 48 61 55 649.0 0 55 62 138 649.0 0 138 63 65 649.0 0 65 64 159 649.0 0 159 65 43 649.0 0 43 66 76 649.0 0 76 67 60 649.0 0 60 68 64 649.0 0 64 69 44 649.0 0 44 70 38 649.0 0 38 71 63 649.0 0 63 72 43 649.0 0 43 73 52 649.0 0 52 74 35 649.0 0 35 75 51 649.0 0 51 76 85 649.0 0 85 77 55 649.0 0 55 78 90 649.0 0 90 79 68 649.0 0 68 80 73 649.0 0 73 81 143 649.0 0 143 82 202 649.0 0 202 83 54 649.0 0 54 84 46 649.0 0 46 85 80 649.0 0 80 86 46 649.0 0 46 87 51 649.0 0 51 88 58 649.0 0 58 89 80 649.0 0 80 90 78 649.0 0 78 91 95 649.0 0 95 92 111 649.0 0 111 93 262 649.0 0 262 94 115 649.0 0 115 95 122 649.0 0 122 96 77 649.0 0 77 97 75 649.0 0 75 98 72 649.0 0 72 99 71 649.0 0 71 100 102 649.0 0 102 101 137 649.0 0 137 102 113 649.0 0 113 103 66 649.0 0 66 104 78 649.0 0 78 105 93 649.0 0 93 106 61 649.0 0 61 107 61 649.0 0 61 108 44 649.0 0 44 109 54 649.0 0 54 110 50 649.0 0 50 111 45 649.0 0 45 112 57 649.0 0 57 113 63 649.0 0 63 114 54 649.0 0 54 115 71 649.0 0 71 116 65 649.0 0 65 117 91 649.0 0 91 118 91 649.0 0 91 119 80 649.0 0 80 120 55 649.0 0 55 121 51 649.0 0 51 122 68 649.0 0 68 123 63 649.0 0 63 124 73 649.0 0 73 125 49 649.0 0 49 126 57 649.0 0 57 127 83 649.0 0 83 128 58 649.0 0 58 129 64 649.0 0 64 130 71 649.0 0 71 131 81 649.0 0 81 132 116 649.0 0 116 133 86 649.0 0 86 134 59 649.0 0 59 135 43 649.0 0 43 136 97 649.0 0 97 137 355 649.0 0 355 138 583 649.0 0 583 139 748 649.0 0 748 140 331 649.0 0 331 141 53 649.0 0 53 142 72 649.0 0 72 143 99 649.0 0 99 144 104 649.0 0 104 145 104 649.0 0 104 146 120 649.0 0 120 147 100 649.0 0 100 148 102 649.0 0 102 149 58 649.0 0 58 150 62 649.0 0 62 151 82 649.0 0 82 152 70 649.0 0 70 153 75 649.0 0 75 154 77 649.0 0 77 155 64 649.0 0 64 156 68 649.0 0 68 157 60 649.0 0 60 158 71 649.0 0 71 159 75 649.0 0 75 160 66 649.0 0 66 161 65 649.0 0 65 162 61 649.0 0 61 163 65 649.0 0 65 164 47 649.0 0 47 165 66 649.0 0 66 166 60 649.0 0 60 167 55 649.0 0 55 168 53 649.0 0 53 169 81 649.0 0 81 170 57 649.0 0 57 171 73 649.0 0 73 172 72 649.0 0 72 173 71 649.0 0 71 174 59 649.0 0 59 175 46 649.0 0 46 176 80 649.0 0 80 177 69 649.0 0 69 178 112 649.0 0 112 179 99 649.0 0 99 180 79 649.0 0 79 181 144 649.0 0 144 182 71 649.0 0 71 183 70 649.0 0 70 184 89 649.0 0 89 185 68 649.0 0 68 186 165 649.0 0 165 187 146 649.0 0 146 188 162 649.0 0 162 189 172 649.0 0 172 190 57 649.0 0 57 191 49 649.0 0 49 192 77 649.0 0 77 193 48 649.0 0 48 194 52 649.0 0 52 195 68 649.0 0 68 196 60 649.0 0 60 197 94 649.0 0 94 198 92 649.0 0 92 199 73 649.0 0 73 200 53 649.0 0 53 201 66 649.0 0 66 202 59 649.0 0 59 203 75 649.0 0 75 204 88 649.0 0 88 205 113 649.0 0 113 206 74 649.0 0 74 207 80 649.0 0 80 208 189 649.0 0 189 209 58 649.0 0 58 210 80 649.0 0 80 211 80 649.0 0 80 212 80 649.0 0 80 213 64 649.0 0 64 214 235 649.0 0 235 215 165 649.0 0 165 216 256 649.0 0 256 217 171 649.0 0 171 218 92 649.0 0 92 219 68 649.0 0 68 220 53 649.0 0 53 221 68 649.0 0 68 222 60 649.0 0 60 223 81 649.0 0 81 224 97 649.0 0 97 225 81 649.0 0 81 226 92 649.0 0 92 227 58 649.0 0 58 228 92 649.0 0 92 229 48 649.0 0 48 230 74 649.0 0 74 231 75 649.0 0 75 232 50 649.0 0 50 233 69 649.0 0 69 234 64 649.0 0 64 235 84 649.0 0 84 236 75 649.0 0 75 237 72 649.0 0 72 238 138 649.0 0 138 239 97 649.0 0 97 240 159 649.0 0 159 241 63 649.0 0 63 242 64 649.0 0 64 243 82 649.0 0 82 244 87 649.0 0 87 245 80 649.0 0 80 246 63 649.0 0 63 247 65 649.0 0 65 248 86 649.0 0 86 249 78 649.0 0 78 250 114 649.0 0 114 251 111 649.0 0 111 252 131 649.0 0 131 253 82 649.0 0 82 254 70 649.0 0 70 255 81 649.0 0 81 256 112 649.0 0 112 257 164 649.0 0 164 258 184 649.0 0 184 259 139 649.0 0 139 260 129 649.0 0 129 261 100 649.0 0 100 262 128 649.0 0 128 263 124 649.0 0 124 264 122 649.0 0 122 265 100 649.0 0 100 266 140 649.0 0 140 267 97 649.0 0 97 268 105 649.0 0 105 269 96 649.0 0 96 270 144 649.0 0 144 271 178 649.0 0 178 272 356 649.0 0 356 273 1494 649.0 0 1494 274 1356 649.0 0 1356 275 3136 649.0 0 3136 276 266 649.0 0 266 277 359 649.0 0 359 278 292 649.0 0 292 279 198 649.0 0 198 280 229 649.0 0 229 281 125 649.0 0 125 282 264 649.0 0 264 283 1150 649.0 0 1150 284 769 649.0 0 769 285 1419 649.0 0 1419 286 1088 649.0 0 1088 287 722 649.0 0 722 288 597 649.0 0 597 289 190 649.0 0 190 290 208 649.0 0 208 291 195 649.0 0 195 292 335 649.0 0 335 293 2556 649.0 0 2556 294 2026 649.0 0 2026 295 1439 649.0 0 1439 296 340 649.0 0 340 297 815 649.0 0 815 298 2490 649.0 0 2490 299 5003 649.0 0 5003 300 4345 649.0 0 4345 301 1578 649.0 0 1578 Finished in 0.93 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 20,565 Reads with adapters: 2,407 (11.7%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 2,397 (11.7%) Total basepairs processed: 4,361,719 bp Total written (filtered): 644,911 bp (14.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2407 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 31 0.0 3 0 26 1 3 1 20 2292 0.0 4 2173 103 10 5 1 21 2 0.0 4 1 1 23 5 0.0 4 0 0 0 3 2 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 1 59 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 94 3 0.0 4 0 0 0 0 3 98 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 1 116 1 0.0 4 0 0 0 0 1 124 1 0.0 4 1 126 1 0.0 4 0 0 0 0 1 127 51 0.0 4 0 0 0 0 51 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 164 1 0.0 4 1 262 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 2,397 Reads with adapters: 564 (23.5%) Reads written (passing filters): 2,397 (100.0%) Total basepairs processed: 644,911 bp Total written (filtered): 627,703 bp (97.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 564 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 8.3% G: 80.1% T: 10.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 66 0.0 0 1 65 10 384 0.0 1 26 358 11 55 0.0 1 2 53 12 2 0.0 1 0 2 13 1 0.0 1 0 1 62 1 0.0 1 1 92 1 0.0 1 1 153 1 0.0 1 1 223 49 0.0 1 43 6 224 2 0.0 1 2 225 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.