This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.33 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 61,427 (2.3%) Reads that were too short: 45,216 (1.7%) Reads written (passing filters): 16,211 (0.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 3,575,800 bp (0.4%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 61427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 56 649.0 0 56 7 88 649.0 0 88 8 2380 649.0 0 2380 9 2610 649.0 0 2610 10 49 649.0 0 49 11 27 649.0 0 27 12 30 649.0 0 30 13 58 649.0 0 58 14 28 649.0 0 28 15 18 649.0 0 18 16 37 649.0 0 37 17 33 649.0 0 33 18 30 649.0 0 30 19 54 649.0 0 54 20 39 649.0 0 39 21 38 649.0 0 38 22 28 649.0 0 28 23 21 649.0 0 21 24 47 649.0 0 47 25 28 649.0 0 28 26 28 649.0 0 28 27 32 649.0 0 32 28 42 649.0 0 42 29 30 649.0 0 30 30 31 649.0 0 31 31 44 649.0 0 44 32 42 649.0 0 42 33 40 649.0 0 40 34 37 649.0 0 37 35 53 649.0 0 53 36 55 649.0 0 55 37 45 649.0 0 45 38 55 649.0 0 55 39 36 649.0 0 36 40 54 649.0 0 54 41 42 649.0 0 42 42 31 649.0 0 31 43 27 649.0 0 27 44 53 649.0 0 53 45 40 649.0 0 40 46 49 649.0 0 49 47 35 649.0 0 35 48 27 649.0 0 27 49 31 649.0 0 31 50 54 649.0 0 54 51 114 649.0 0 114 52 56 649.0 0 56 53 74 649.0 0 74 54 36 649.0 0 36 55 58 649.0 0 58 56 30 649.0 0 30 57 26 649.0 0 26 58 39 649.0 0 39 59 33 649.0 0 33 60 48 649.0 0 48 61 49 649.0 0 49 62 91 649.0 0 91 63 36 649.0 0 36 64 126 649.0 0 126 65 48 649.0 0 48 66 50 649.0 0 50 67 59 649.0 0 59 68 67 649.0 0 67 69 54 649.0 0 54 70 40 649.0 0 40 71 38 649.0 0 38 72 35 649.0 0 35 73 41 649.0 0 41 74 51 649.0 0 51 75 45 649.0 0 45 76 34 649.0 0 34 77 41 649.0 0 41 78 44 649.0 0 44 79 52 649.0 0 52 80 44 649.0 0 44 81 98 649.0 0 98 82 196 649.0 0 196 83 29 649.0 0 29 84 35 649.0 0 35 85 51 649.0 0 51 86 38 649.0 0 38 87 34 649.0 0 34 88 30 649.0 0 30 89 55 649.0 0 55 90 55 649.0 0 55 91 49 649.0 0 49 92 94 649.0 0 94 93 215 649.0 0 215 94 78 649.0 0 78 95 53 649.0 0 53 96 72 649.0 0 72 97 66 649.0 0 66 98 49 649.0 0 49 99 74 649.0 0 74 100 98 649.0 0 98 101 86 649.0 0 86 102 101 649.0 0 101 103 45 649.0 0 45 104 45 649.0 0 45 105 62 649.0 0 62 106 37 649.0 0 37 107 50 649.0 0 50 108 43 649.0 0 43 109 63 649.0 0 63 110 51 649.0 0 51 111 31 649.0 0 31 112 41 649.0 0 41 113 43 649.0 0 43 114 50 649.0 0 50 115 90 649.0 0 90 116 31 649.0 0 31 117 63 649.0 0 63 118 64 649.0 0 64 119 63 649.0 0 63 120 41 649.0 0 41 121 39 649.0 0 39 122 43 649.0 0 43 123 45 649.0 0 45 124 48 649.0 0 48 125 42 649.0 0 42 126 33 649.0 0 33 127 92 649.0 0 92 128 55 649.0 0 55 129 48 649.0 0 48 130 63 649.0 0 63 131 56 649.0 0 56 132 51 649.0 0 51 133 42 649.0 0 42 134 44 649.0 0 44 135 38 649.0 0 38 136 58 649.0 0 58 137 131 649.0 0 131 138 346 649.0 0 346 139 354 649.0 0 354 140 217 649.0 0 217 141 38 649.0 0 38 142 45 649.0 0 45 143 47 649.0 0 47 144 76 649.0 0 76 145 58 649.0 0 58 146 60 649.0 0 60 147 59 649.0 0 59 148 56 649.0 0 56 149 39 649.0 0 39 150 35 649.0 0 35 151 67 649.0 0 67 152 49 649.0 0 49 153 48 649.0 0 48 154 49 649.0 0 49 155 48 649.0 0 48 156 40 649.0 0 40 157 53 649.0 0 53 158 53 649.0 0 53 159 50 649.0 0 50 160 38 649.0 0 38 161 40 649.0 0 40 162 49 649.0 0 49 163 49 649.0 0 49 164 47 649.0 0 47 165 59 649.0 0 59 166 50 649.0 0 50 167 47 649.0 0 47 168 43 649.0 0 43 169 40 649.0 0 40 170 48 649.0 0 48 171 72 649.0 0 72 172 53 649.0 0 53 173 56 649.0 0 56 174 51 649.0 0 51 175 44 649.0 0 44 176 64 649.0 0 64 177 51 649.0 0 51 178 110 649.0 0 110 179 83 649.0 0 83 180 58 649.0 0 58 181 128 649.0 0 128 182 42 649.0 0 42 183 59 649.0 0 59 184 79 649.0 0 79 185 73 649.0 0 73 186 102 649.0 0 102 187 88 649.0 0 88 188 111 649.0 0 111 189 82 649.0 0 82 190 47 649.0 0 47 191 59 649.0 0 59 192 47 649.0 0 47 193 36 649.0 0 36 194 56 649.0 0 56 195 60 649.0 0 60 196 51 649.0 0 51 197 65 649.0 0 65 198 68 649.0 0 68 199 44 649.0 0 44 200 53 649.0 0 53 201 54 649.0 0 54 202 53 649.0 0 53 203 60 649.0 0 60 204 48 649.0 0 48 205 45 649.0 0 45 206 53 649.0 0 53 207 56 649.0 0 56 208 201 649.0 0 201 209 66 649.0 0 66 210 57 649.0 0 57 211 53 649.0 0 53 212 60 649.0 0 60 213 60 649.0 0 60 214 93 649.0 0 93 215 94 649.0 0 94 216 107 649.0 0 107 217 155 649.0 0 155 218 52 649.0 0 52 219 43 649.0 0 43 220 59 649.0 0 59 221 48 649.0 0 48 222 42 649.0 0 42 223 52 649.0 0 52 224 67 649.0 0 67 225 58 649.0 0 58 226 64 649.0 0 64 227 49 649.0 0 49 228 60 649.0 0 60 229 49 649.0 0 49 230 50 649.0 0 50 231 60 649.0 0 60 232 53 649.0 0 53 233 64 649.0 0 64 234 58 649.0 0 58 235 50 649.0 0 50 236 58 649.0 0 58 237 39 649.0 0 39 238 93 649.0 0 93 239 67 649.0 0 67 240 87 649.0 0 87 241 52 649.0 0 52 242 56 649.0 0 56 243 57 649.0 0 57 244 53 649.0 0 53 245 66 649.0 0 66 246 57 649.0 0 57 247 50 649.0 0 50 248 66 649.0 0 66 249 84 649.0 0 84 250 55 649.0 0 55 251 66 649.0 0 66 252 54 649.0 0 54 253 56 649.0 0 56 254 62 649.0 0 62 255 78 649.0 0 78 256 55 649.0 0 55 257 103 649.0 0 103 258 83 649.0 0 83 259 80 649.0 0 80 260 103 649.0 0 103 261 67 649.0 0 67 262 95 649.0 0 95 263 86 649.0 0 86 264 72 649.0 0 72 265 67 649.0 0 67 266 95 649.0 0 95 267 95 649.0 0 95 268 91 649.0 0 91 269 93 649.0 0 93 270 114 649.0 0 114 271 114 649.0 0 114 272 268 649.0 0 268 273 1542 649.0 0 1542 274 1403 649.0 0 1403 275 3699 649.0 0 3699 276 112 649.0 0 112 277 94 649.0 0 94 278 114 649.0 0 114 279 127 649.0 0 127 280 143 649.0 0 143 281 83 649.0 0 83 282 204 649.0 0 204 283 519 649.0 0 519 284 357 649.0 0 357 285 700 649.0 0 700 286 584 649.0 0 584 287 454 649.0 0 454 288 299 649.0 0 299 289 123 649.0 0 123 290 133 649.0 0 133 291 164 649.0 0 164 292 304 649.0 0 304 293 3857 649.0 0 3857 294 2911 649.0 0 2911 295 2139 649.0 0 2139 296 247 649.0 0 247 297 778 649.0 0 778 298 2235 649.0 0 2235 299 6077 649.0 0 6077 300 7154 649.0 0 7154 301 3484 649.0 0 3484 Finished in 0.70 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 16,211 Reads with adapters: 2,385 (14.7%) Reads that were too short: 10 (0.1%) Reads written (passing filters): 2,375 (14.7%) Total basepairs processed: 3,575,800 bp Total written (filtered): 650,640 bp (18.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2385 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 17 0.0 3 0 14 3 17 2298 0.0 3 2240 56 2 18 7 0.0 3 1 6 19 6 0.0 3 2 0 4 20 3 0.0 3 1 0 0 2 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 24 5 0.0 3 0 0 0 5 25 1 0.0 3 0 0 0 1 35 2 0.0 3 1 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 1 48 2 0.0 3 0 0 0 2 55 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 8 0.0 3 0 0 0 8 77 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 157 1 0.0 3 1 198 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 2,375 Reads with adapters: 37 (1.6%) Reads written (passing filters): 2,375 (100.0%) Total basepairs processed: 650,640 bp Total written (filtered): 650,505 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.6% C: 5.4% G: 67.6% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 13 37.1 0 13 4 24 9.3 0 24