This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.60 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 147,286 (5.5%) Reads that were too short: 12,954 (0.5%) Reads written (passing filters): 134,332 (5.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 36,037,179 bp (4.5%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 147286 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4564 649.0 0 4564 7 43801 649.0 0 43801 8 51171 649.0 0 51171 9 67 649.0 0 67 10 31 649.0 0 31 11 38 649.0 0 38 12 36 649.0 0 36 13 25 649.0 0 25 14 38 649.0 0 38 15 31 649.0 0 31 16 25 649.0 0 25 17 30 649.0 0 30 18 27 649.0 0 27 19 13 649.0 0 13 20 26 649.0 0 26 21 34 649.0 0 34 22 19 649.0 0 19 23 38 649.0 0 38 24 31 649.0 0 31 25 32 649.0 0 32 26 38 649.0 0 38 27 33 649.0 0 33 28 38 649.0 0 38 29 22 649.0 0 22 30 50 649.0 0 50 31 114 649.0 0 114 32 70 649.0 0 70 33 74 649.0 0 74 34 99 649.0 0 99 35 47 649.0 0 47 36 40 649.0 0 40 37 91 649.0 0 91 38 41 649.0 0 41 39 61 649.0 0 61 40 54 649.0 0 54 41 64 649.0 0 64 42 44 649.0 0 44 43 72 649.0 0 72 44 74 649.0 0 74 45 71 649.0 0 71 46 76 649.0 0 76 47 114 649.0 0 114 48 143 649.0 0 143 49 206 649.0 0 206 50 5655 649.0 0 5655 51 1454 649.0 0 1454 52 5180 649.0 0 5180 53 43 649.0 0 43 54 54 649.0 0 54 55 25 649.0 0 25 56 16 649.0 0 16 57 30 649.0 0 30 58 34 649.0 0 34 59 29 649.0 0 29 60 30 649.0 0 30 61 30 649.0 0 30 62 25 649.0 0 25 63 39 649.0 0 39 64 44 649.0 0 44 65 159 649.0 0 159 66 43 649.0 0 43 67 31 649.0 0 31 68 61 649.0 0 61 69 42 649.0 0 42 70 25 649.0 0 25 71 36 649.0 0 36 72 53 649.0 0 53 73 53 649.0 0 53 74 266 649.0 0 266 75 194 649.0 0 194 76 778 649.0 0 778 77 43 649.0 0 43 78 93 649.0 0 93 79 46 649.0 0 46 80 70 649.0 0 70 81 35 649.0 0 35 82 37 649.0 0 37 83 21 649.0 0 21 84 23 649.0 0 23 85 39 649.0 0 39 86 36 649.0 0 36 87 30 649.0 0 30 88 26 649.0 0 26 89 30 649.0 0 30 90 39 649.0 0 39 91 40 649.0 0 40 92 36 649.0 0 36 93 57 649.0 0 57 94 47 649.0 0 47 95 47 649.0 0 47 96 40 649.0 0 40 97 34 649.0 0 34 98 60 649.0 0 60 99 46 649.0 0 46 100 41 649.0 0 41 101 40 649.0 0 40 102 47 649.0 0 47 103 45 649.0 0 45 104 54 649.0 0 54 105 30 649.0 0 30 106 35 649.0 0 35 107 35 649.0 0 35 108 37 649.0 0 37 109 34 649.0 0 34 110 38 649.0 0 38 111 46 649.0 0 46 112 54 649.0 0 54 113 37 649.0 0 37 114 89 649.0 0 89 115 102 649.0 0 102 116 85 649.0 0 85 117 92 649.0 0 92 118 74 649.0 0 74 119 71 649.0 0 71 120 70 649.0 0 70 121 45 649.0 0 45 122 73 649.0 0 73 123 67 649.0 0 67 124 101 649.0 0 101 125 983 649.0 0 983 126 474 649.0 0 474 127 1602 649.0 0 1602 128 76 649.0 0 76 129 56 649.0 0 56 130 135 649.0 0 135 131 85 649.0 0 85 132 156 649.0 0 156 133 38 649.0 0 38 134 52 649.0 0 52 135 226 649.0 0 226 136 148 649.0 0 148 137 221 649.0 0 221 138 46 649.0 0 46 139 34 649.0 0 34 140 33 649.0 0 33 141 47 649.0 0 47 142 38 649.0 0 38 143 38 649.0 0 38 144 40 649.0 0 40 145 38 649.0 0 38 146 41 649.0 0 41 147 44 649.0 0 44 148 51 649.0 0 51 149 44 649.0 0 44 150 52 649.0 0 52 151 67 649.0 0 67 152 46 649.0 0 46 153 57 649.0 0 57 154 77 649.0 0 77 155 53 649.0 0 53 156 50 649.0 0 50 157 57 649.0 0 57 158 52 649.0 0 52 159 64 649.0 0 64 160 62 649.0 0 62 161 96 649.0 0 96 162 95 649.0 0 95 163 121 649.0 0 121 164 1579 649.0 0 1579 165 748 649.0 0 748 166 2471 649.0 0 2471 167 52 649.0 0 52 168 49 649.0 0 49 169 43 649.0 0 43 170 63 649.0 0 63 171 41 649.0 0 41 172 64 649.0 0 64 173 37 649.0 0 37 174 34 649.0 0 34 175 33 649.0 0 33 176 43 649.0 0 43 177 54 649.0 0 54 178 53 649.0 0 53 179 49 649.0 0 49 180 50 649.0 0 50 181 59 649.0 0 59 182 53 649.0 0 53 183 55 649.0 0 55 184 65 649.0 0 65 185 46 649.0 0 46 186 61 649.0 0 61 187 55 649.0 0 55 188 59 649.0 0 59 189 133 649.0 0 133 190 105 649.0 0 105 191 779 649.0 0 779 192 251 649.0 0 251 193 448 649.0 0 448 194 92 649.0 0 92 195 78 649.0 0 78 196 587 649.0 0 587 197 523 649.0 0 523 198 531 649.0 0 531 199 319 649.0 0 319 200 135 649.0 0 135 201 81 649.0 0 81 202 65 649.0 0 65 203 51 649.0 0 51 204 146 649.0 0 146 205 90 649.0 0 90 206 149 649.0 0 149 207 64 649.0 0 64 208 105 649.0 0 105 209 76 649.0 0 76 210 85 649.0 0 85 211 92 649.0 0 92 212 52 649.0 0 52 213 116 649.0 0 116 214 78 649.0 0 78 215 92 649.0 0 92 216 248 649.0 0 248 217 445 649.0 0 445 218 305 649.0 0 305 219 180 649.0 0 180 220 87 649.0 0 87 221 74 649.0 0 74 222 61 649.0 0 61 223 54 649.0 0 54 224 49 649.0 0 49 225 100 649.0 0 100 226 61 649.0 0 61 227 62 649.0 0 62 228 57 649.0 0 57 229 46 649.0 0 46 230 67 649.0 0 67 231 72 649.0 0 72 232 72 649.0 0 72 233 50 649.0 0 50 234 65 649.0 0 65 235 44 649.0 0 44 236 60 649.0 0 60 237 59 649.0 0 59 238 93 649.0 0 93 239 127 649.0 0 127 240 136 649.0 0 136 241 119 649.0 0 119 242 78 649.0 0 78 243 77 649.0 0 77 244 42 649.0 0 42 245 61 649.0 0 61 246 86 649.0 0 86 247 369 649.0 0 369 248 487 649.0 0 487 249 754 649.0 0 754 250 1109 649.0 0 1109 251 181 649.0 0 181 252 113 649.0 0 113 253 68 649.0 0 68 254 64 649.0 0 64 255 61 649.0 0 61 256 64 649.0 0 64 257 71 649.0 0 71 258 75 649.0 0 75 259 72 649.0 0 72 260 77 649.0 0 77 261 56 649.0 0 56 262 62 649.0 0 62 263 100 649.0 0 100 264 75 649.0 0 75 265 69 649.0 0 69 266 58 649.0 0 58 267 68 649.0 0 68 268 64 649.0 0 64 269 555 649.0 0 555 270 166 649.0 0 166 271 123 649.0 0 123 272 53 649.0 0 53 273 71 649.0 0 71 274 64 649.0 0 64 275 48 649.0 0 48 276 57 649.0 0 57 277 62 649.0 0 62 278 65 649.0 0 65 279 75 649.0 0 75 280 59 649.0 0 59 281 66 649.0 0 66 282 69 649.0 0 69 283 64 649.0 0 64 284 74 649.0 0 74 285 71 649.0 0 71 286 67 649.0 0 67 287 65 649.0 0 65 288 75 649.0 0 75 289 263 649.0 0 263 290 338 649.0 0 338 291 434 649.0 0 434 292 324 649.0 0 324 293 125 649.0 0 125 294 72 649.0 0 72 295 55 649.0 0 55 296 75 649.0 0 75 297 155 649.0 0 155 298 185 649.0 0 185 299 208 649.0 0 208 300 102 649.0 0 102 301 154 649.0 0 154 Finished in 6.21 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 134,332 Reads with adapters: 45,961 (34.2%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 45,920 (34.2%) Total basepairs processed: 36,037,179 bp Total written (filtered): 12,696,795 bp (35.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 45961 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 13 0.0 3 0 0 12 1 16 270 0.0 3 0 266 2 2 17 45317 0.0 3 44320 954 40 3 18 100 0.0 3 29 68 2 1 19 14 0.0 3 0 0 12 2 20 42 0.0 3 0 0 0 42 25 1 0.0 3 0 0 0 1 27 37 0.0 3 0 0 0 37 29 1 0.0 3 0 0 0 1 30 5 0.0 3 0 0 0 5 35 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 46 37 0.0 3 0 0 0 37 49 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 1 2 63 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 75 21 0.0 3 0 0 0 21 78 1 0.0 3 0 0 0 1 87 5 0.0 3 0 0 0 5 88 2 0.0 3 0 0 0 2 90 4 0.0 3 0 0 0 4 92 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 12 0.0 3 1 0 0 11 118 6 0.0 3 0 0 0 6 120 6 0.0 3 0 0 0 6 121 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 1 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 161 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.63 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 45,920 Reads with adapters: 146 (0.3%) Reads written (passing filters): 45,920 (100.0%) Total basepairs processed: 12,696,795 bp Total written (filtered): 12,696,338 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 146 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.8% C: 17.1% G: 30.8% T: 10.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 130 717.5 0 130 4 13 179.4 0 13 5 3 44.8 0 3