This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.42 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 129,795 (4.9%) Reads that were too short: 85,677 (3.2%) Reads written (passing filters): 44,118 (1.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 7,667,138 bp (1.0%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 129795 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 463 649.0 0 463 7 71 649.0 0 71 8 70 649.0 0 70 9 50 649.0 0 50 10 293 649.0 0 293 11 34 649.0 0 34 12 50 649.0 0 50 13 32 649.0 0 32 14 33 649.0 0 33 15 61 649.0 0 61 16 107 649.0 0 107 17 63 649.0 0 63 18 56 649.0 0 56 19 42 649.0 0 42 20 30 649.0 0 30 21 32 649.0 0 32 22 40 649.0 0 40 23 44 649.0 0 44 24 31 649.0 0 31 25 36 649.0 0 36 26 40 649.0 0 40 27 43 649.0 0 43 28 59 649.0 0 59 29 40 649.0 0 40 30 48 649.0 0 48 31 39 649.0 0 39 32 55 649.0 0 55 33 41 649.0 0 41 34 49 649.0 0 49 35 64 649.0 0 64 36 36 649.0 0 36 37 97 649.0 0 97 38 106 649.0 0 106 39 90 649.0 0 90 40 735 649.0 0 735 41 340 649.0 0 340 42 76 649.0 0 76 43 46 649.0 0 46 44 36 649.0 0 36 45 43 649.0 0 43 46 36 649.0 0 36 47 40 649.0 0 40 48 51 649.0 0 51 49 50 649.0 0 50 50 71 649.0 0 71 51 58 649.0 0 58 52 51 649.0 0 51 53 61 649.0 0 61 54 55 649.0 0 55 55 100 649.0 0 100 56 237 649.0 0 237 57 2002 649.0 0 2002 58 495 649.0 0 495 59 444 649.0 0 444 60 38 649.0 0 38 61 37 649.0 0 37 62 32 649.0 0 32 63 52 649.0 0 52 64 35 649.0 0 35 65 53 649.0 0 53 66 50 649.0 0 50 67 50 649.0 0 50 68 62 649.0 0 62 69 62 649.0 0 62 70 41 649.0 0 41 71 41 649.0 0 41 72 45 649.0 0 45 73 56 649.0 0 56 74 68 649.0 0 68 75 107 649.0 0 107 76 84 649.0 0 84 77 48 649.0 0 48 78 88 649.0 0 88 79 93 649.0 0 93 80 107 649.0 0 107 81 68 649.0 0 68 82 109 649.0 0 109 83 100 649.0 0 100 84 92 649.0 0 92 85 56 649.0 0 56 86 62 649.0 0 62 87 59 649.0 0 59 88 164 649.0 0 164 89 120 649.0 0 120 90 124 649.0 0 124 91 117 649.0 0 117 92 81 649.0 0 81 93 92 649.0 0 92 94 70 649.0 0 70 95 62 649.0 0 62 96 88 649.0 0 88 97 71 649.0 0 71 98 76 649.0 0 76 99 85 649.0 0 85 100 125 649.0 0 125 101 115 649.0 0 115 102 80 649.0 0 80 103 93 649.0 0 93 104 165 649.0 0 165 105 205 649.0 0 205 106 151 649.0 0 151 107 280 649.0 0 280 108 134 649.0 0 134 109 192 649.0 0 192 110 97 649.0 0 97 111 56 649.0 0 56 112 63 649.0 0 63 113 84 649.0 0 84 114 75 649.0 0 75 115 117 649.0 0 117 116 106 649.0 0 106 117 122 649.0 0 122 118 99 649.0 0 99 119 116 649.0 0 116 120 170 649.0 0 170 121 356 649.0 0 356 122 1747 649.0 0 1747 123 695 649.0 0 695 124 772 649.0 0 772 125 103 649.0 0 103 126 135 649.0 0 135 127 211 649.0 0 211 128 148 649.0 0 148 129 117 649.0 0 117 130 223 649.0 0 223 131 144 649.0 0 144 132 128 649.0 0 128 133 111 649.0 0 111 134 130 649.0 0 130 135 153 649.0 0 153 136 201 649.0 0 201 137 158 649.0 0 158 138 112 649.0 0 112 139 104 649.0 0 104 140 151 649.0 0 151 141 523 649.0 0 523 142 450 649.0 0 450 143 367 649.0 0 367 144 199 649.0 0 199 145 138 649.0 0 138 146 160 649.0 0 160 147 292 649.0 0 292 148 279 649.0 0 279 149 393 649.0 0 393 150 395 649.0 0 395 151 3125 649.0 0 3125 152 5262 649.0 0 5262 153 4087 649.0 0 4087 154 164 649.0 0 164 155 151 649.0 0 151 156 169 649.0 0 169 157 772 649.0 0 772 158 337 649.0 0 337 159 381 649.0 0 381 160 159 649.0 0 159 161 292 649.0 0 292 162 473 649.0 0 473 163 354 649.0 0 354 164 155 649.0 0 155 165 116 649.0 0 116 166 124 649.0 0 124 167 158 649.0 0 158 168 151 649.0 0 151 169 130 649.0 0 130 170 108 649.0 0 108 171 84 649.0 0 84 172 67 649.0 0 67 173 101 649.0 0 101 174 108 649.0 0 108 175 142 649.0 0 142 176 244 649.0 0 244 177 189 649.0 0 189 178 147 649.0 0 147 179 87 649.0 0 87 180 88 649.0 0 88 181 111 649.0 0 111 182 320 649.0 0 320 183 262 649.0 0 262 184 254 649.0 0 254 185 147 649.0 0 147 186 198 649.0 0 198 187 283 649.0 0 283 188 286 649.0 0 286 189 213 649.0 0 213 190 102 649.0 0 102 191 89 649.0 0 89 192 81 649.0 0 81 193 116 649.0 0 116 194 181 649.0 0 181 195 265 649.0 0 265 196 328 649.0 0 328 197 198 649.0 0 198 198 163 649.0 0 163 199 104 649.0 0 104 200 135 649.0 0 135 201 200 649.0 0 200 202 181 649.0 0 181 203 166 649.0 0 166 204 160 649.0 0 160 205 206 649.0 0 206 206 183 649.0 0 183 207 286 649.0 0 286 208 559 649.0 0 559 209 1596 649.0 0 1596 210 1339 649.0 0 1339 211 1514 649.0 0 1514 212 2916 649.0 0 2916 213 26388 649.0 0 26388 214 8736 649.0 0 8736 215 11337 649.0 0 11337 216 562 649.0 0 562 217 216 649.0 0 216 218 196 649.0 0 196 219 240 649.0 0 240 220 653 649.0 0 653 221 2805 649.0 0 2805 222 3248 649.0 0 3248 223 2441 649.0 0 2441 224 293 649.0 0 293 225 132 649.0 0 132 226 160 649.0 0 160 227 139 649.0 0 139 228 120 649.0 0 120 229 110 649.0 0 110 230 87 649.0 0 87 231 209 649.0 0 209 232 210 649.0 0 210 233 211 649.0 0 211 234 193 649.0 0 193 235 252 649.0 0 252 236 170 649.0 0 170 237 163 649.0 0 163 238 180 649.0 0 180 239 185 649.0 0 185 240 163 649.0 0 163 241 136 649.0 0 136 242 213 649.0 0 213 243 263 649.0 0 263 244 393 649.0 0 393 245 332 649.0 0 332 246 203 649.0 0 203 247 102 649.0 0 102 248 101 649.0 0 101 249 114 649.0 0 114 250 122 649.0 0 122 251 119 649.0 0 119 252 112 649.0 0 112 253 141 649.0 0 141 254 158 649.0 0 158 255 187 649.0 0 187 256 325 649.0 0 325 257 672 649.0 0 672 258 560 649.0 0 560 259 531 649.0 0 531 260 273 649.0 0 273 261 223 649.0 0 223 262 251 649.0 0 251 263 608 649.0 0 608 264 706 649.0 0 706 265 625 649.0 0 625 266 215 649.0 0 215 267 128 649.0 0 128 268 152 649.0 0 152 269 246 649.0 0 246 270 397 649.0 0 397 271 323 649.0 0 323 272 277 649.0 0 277 273 179 649.0 0 179 274 445 649.0 0 445 275 446 649.0 0 446 276 298 649.0 0 298 277 314 649.0 0 314 278 257 649.0 0 257 279 168 649.0 0 168 280 143 649.0 0 143 281 268 649.0 0 268 282 275 649.0 0 275 283 408 649.0 0 408 284 366 649.0 0 366 285 357 649.0 0 357 286 283 649.0 0 283 287 141 649.0 0 141 288 176 649.0 0 176 289 301 649.0 0 301 290 344 649.0 0 344 291 362 649.0 0 362 292 185 649.0 0 185 293 110 649.0 0 110 294 152 649.0 0 152 295 203 649.0 0 203 296 319 649.0 0 319 297 267 649.0 0 267 298 300 649.0 0 300 299 204 649.0 0 204 300 142 649.0 0 142 301 151 649.0 0 151 Finished in 1.71 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 44,118 Reads with adapters: 602 (1.4%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 585 (1.3%) Total basepairs processed: 7,667,138 bp Total written (filtered): 137,757 bp (1.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 602 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 2 0.0 3 0 1 1 20 23 0.0 4 15 2 5 0 1 21 1 0.0 4 1 22 9 0.0 4 0 9 23 398 0.0 4 376 14 4 3 1 26 3 0.0 4 0 0 0 2 1 27 137 0.0 4 0 0 0 0 137 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 1 50 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 1 79 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 1 218 1 0.0 4 0 0 1 219 1 0.0 4 0 0 0 1 222 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 585 Reads with adapters: 104 (17.8%) Reads written (passing filters): 585 (100.0%) Total basepairs processed: 137,757 bp Total written (filtered): 136,370 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 104 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 6.7% G: 89.4% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 28 0.0 0 1 27 10 62 0.0 1 4 58 11 10 0.0 1 0 10 13 2 0.0 1 0 2 171 1 0.0 1 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.