This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.31 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 118,931 (4.5%) Reads that were too short: 78,377 (2.9%) Reads written (passing filters): 40,554 (1.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 7,116,949 bp (0.9%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 118931 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 526 649.0 0 526 7 77 649.0 0 77 8 32 649.0 0 32 9 60 649.0 0 60 10 159 649.0 0 159 11 19 649.0 0 19 12 14 649.0 0 14 13 18 649.0 0 18 14 36 649.0 0 36 15 35 649.0 0 35 16 31 649.0 0 31 17 33 649.0 0 33 18 51 649.0 0 51 19 37 649.0 0 37 20 28 649.0 0 28 21 18 649.0 0 18 22 30 649.0 0 30 23 39 649.0 0 39 24 24 649.0 0 24 25 22 649.0 0 22 26 39 649.0 0 39 27 656 649.0 0 656 28 68 649.0 0 68 29 25 649.0 0 25 30 29 649.0 0 29 31 31 649.0 0 31 32 37 649.0 0 37 33 33 649.0 0 33 34 36 649.0 0 36 35 44 649.0 0 44 36 40 649.0 0 40 37 81 649.0 0 81 38 71 649.0 0 71 39 62 649.0 0 62 40 736 649.0 0 736 41 383 649.0 0 383 42 75 649.0 0 75 43 31 649.0 0 31 44 33 649.0 0 33 45 35 649.0 0 35 46 34 649.0 0 34 47 29 649.0 0 29 48 41 649.0 0 41 49 22 649.0 0 22 50 57 649.0 0 57 51 49 649.0 0 49 52 47 649.0 0 47 53 49 649.0 0 49 54 57 649.0 0 57 55 95 649.0 0 95 56 201 649.0 0 201 57 2178 649.0 0 2178 58 476 649.0 0 476 59 380 649.0 0 380 60 30 649.0 0 30 61 28 649.0 0 28 62 23 649.0 0 23 63 36 649.0 0 36 64 31 649.0 0 31 65 33 649.0 0 33 66 29 649.0 0 29 67 29 649.0 0 29 68 41 649.0 0 41 69 48 649.0 0 48 70 29 649.0 0 29 71 35 649.0 0 35 72 24 649.0 0 24 73 31 649.0 0 31 74 44 649.0 0 44 75 38 649.0 0 38 76 32 649.0 0 32 77 40 649.0 0 40 78 74 649.0 0 74 79 103 649.0 0 103 80 112 649.0 0 112 81 85 649.0 0 85 82 125 649.0 0 125 83 92 649.0 0 92 84 124 649.0 0 124 85 66 649.0 0 66 86 75 649.0 0 75 87 70 649.0 0 70 88 370 649.0 0 370 89 209 649.0 0 209 90 279 649.0 0 279 91 128 649.0 0 128 92 205 649.0 0 205 93 98 649.0 0 98 94 47 649.0 0 47 95 69 649.0 0 69 96 58 649.0 0 58 97 56 649.0 0 56 98 114 649.0 0 114 99 79 649.0 0 79 100 63 649.0 0 63 101 100 649.0 0 100 102 100 649.0 0 100 103 114 649.0 0 114 104 145 649.0 0 145 105 179 649.0 0 179 106 185 649.0 0 185 107 161 649.0 0 161 108 115 649.0 0 115 109 163 649.0 0 163 110 93 649.0 0 93 111 63 649.0 0 63 112 54 649.0 0 54 113 65 649.0 0 65 114 70 649.0 0 70 115 119 649.0 0 119 116 76 649.0 0 76 117 109 649.0 0 109 118 63 649.0 0 63 119 62 649.0 0 62 120 92 649.0 0 92 121 153 649.0 0 153 122 728 649.0 0 728 123 494 649.0 0 494 124 453 649.0 0 453 125 73 649.0 0 73 126 103 649.0 0 103 127 172 649.0 0 172 128 130 649.0 0 130 129 109 649.0 0 109 130 173 649.0 0 173 131 102 649.0 0 102 132 107 649.0 0 107 133 105 649.0 0 105 134 148 649.0 0 148 135 154 649.0 0 154 136 124 649.0 0 124 137 118 649.0 0 118 138 105 649.0 0 105 139 102 649.0 0 102 140 106 649.0 0 106 141 399 649.0 0 399 142 340 649.0 0 340 143 301 649.0 0 301 144 140 649.0 0 140 145 106 649.0 0 106 146 154 649.0 0 154 147 225 649.0 0 225 148 245 649.0 0 245 149 312 649.0 0 312 150 389 649.0 0 389 151 3247 649.0 0 3247 152 5731 649.0 0 5731 153 4431 649.0 0 4431 154 119 649.0 0 119 155 145 649.0 0 145 156 173 649.0 0 173 157 890 649.0 0 890 158 299 649.0 0 299 159 352 649.0 0 352 160 108 649.0 0 108 161 220 649.0 0 220 162 285 649.0 0 285 163 241 649.0 0 241 164 114 649.0 0 114 165 90 649.0 0 90 166 113 649.0 0 113 167 124 649.0 0 124 168 100 649.0 0 100 169 106 649.0 0 106 170 93 649.0 0 93 171 82 649.0 0 82 172 60 649.0 0 60 173 80 649.0 0 80 174 84 649.0 0 84 175 120 649.0 0 120 176 163 649.0 0 163 177 128 649.0 0 128 178 110 649.0 0 110 179 89 649.0 0 89 180 78 649.0 0 78 181 101 649.0 0 101 182 262 649.0 0 262 183 203 649.0 0 203 184 194 649.0 0 194 185 108 649.0 0 108 186 174 649.0 0 174 187 214 649.0 0 214 188 192 649.0 0 192 189 142 649.0 0 142 190 83 649.0 0 83 191 84 649.0 0 84 192 79 649.0 0 79 193 94 649.0 0 94 194 144 649.0 0 144 195 247 649.0 0 247 196 244 649.0 0 244 197 134 649.0 0 134 198 121 649.0 0 121 199 97 649.0 0 97 200 131 649.0 0 131 201 182 649.0 0 182 202 149 649.0 0 149 203 125 649.0 0 125 204 122 649.0 0 122 205 142 649.0 0 142 206 187 649.0 0 187 207 224 649.0 0 224 208 371 649.0 0 371 209 906 649.0 0 906 210 820 649.0 0 820 211 1351 649.0 0 1351 212 2739 649.0 0 2739 213 28755 649.0 0 28755 214 10055 649.0 0 10055 215 13101 649.0 0 13101 216 581 649.0 0 581 217 180 649.0 0 180 218 114 649.0 0 114 219 146 649.0 0 146 220 351 649.0 0 351 221 1487 649.0 0 1487 222 1469 649.0 0 1469 223 1176 649.0 0 1176 224 193 649.0 0 193 225 129 649.0 0 129 226 106 649.0 0 106 227 109 649.0 0 109 228 105 649.0 0 105 229 91 649.0 0 91 230 80 649.0 0 80 231 143 649.0 0 143 232 143 649.0 0 143 233 151 649.0 0 151 234 149 649.0 0 149 235 235 649.0 0 235 236 148 649.0 0 148 237 110 649.0 0 110 238 158 649.0 0 158 239 145 649.0 0 145 240 119 649.0 0 119 241 112 649.0 0 112 242 169 649.0 0 169 243 166 649.0 0 166 244 214 649.0 0 214 245 159 649.0 0 159 246 116 649.0 0 116 247 85 649.0 0 85 248 99 649.0 0 99 249 94 649.0 0 94 250 104 649.0 0 104 251 95 649.0 0 95 252 101 649.0 0 101 253 119 649.0 0 119 254 114 649.0 0 114 255 167 649.0 0 167 256 201 649.0 0 201 257 347 649.0 0 347 258 336 649.0 0 336 259 343 649.0 0 343 260 233 649.0 0 233 261 149 649.0 0 149 262 169 649.0 0 169 263 343 649.0 0 343 264 346 649.0 0 346 265 325 649.0 0 325 266 179 649.0 0 179 267 76 649.0 0 76 268 97 649.0 0 97 269 189 649.0 0 189 270 272 649.0 0 272 271 185 649.0 0 185 272 240 649.0 0 240 273 175 649.0 0 175 274 456 649.0 0 456 275 346 649.0 0 346 276 227 649.0 0 227 277 215 649.0 0 215 278 171 649.0 0 171 279 151 649.0 0 151 280 117 649.0 0 117 281 187 649.0 0 187 282 196 649.0 0 196 283 282 649.0 0 282 284 229 649.0 0 229 285 198 649.0 0 198 286 186 649.0 0 186 287 138 649.0 0 138 288 132 649.0 0 132 289 184 649.0 0 184 290 251 649.0 0 251 291 204 649.0 0 204 292 134 649.0 0 134 293 100 649.0 0 100 294 154 649.0 0 154 295 177 649.0 0 177 296 231 649.0 0 231 297 211 649.0 0 211 298 214 649.0 0 214 299 126 649.0 0 126 300 93 649.0 0 93 301 83 649.0 0 83 Finished in 1.46 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 40,554 Reads with adapters: 1,262 (3.1%) Reads that were too short: 99 (0.2%) Reads written (passing filters): 1,163 (2.9%) Total basepairs processed: 7,116,949 bp Total written (filtered): 127,104 bp (1.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1262 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 1 17 43 0.0 3 43 19 2 0.0 3 1 0 0 1 32 6 0.0 3 0 0 0 6 42 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 70 5 0.0 3 0 0 0 5 77 1 0.0 3 0 0 0 1 80 1 0.0 3 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 94 1 0.0 3 1 100 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1159 0.0 3 0 0 3 1156 162 6 0.0 3 0 0 0 6 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 1 212 1 0.0 3 1 213 1 0.0 3 0 1 214 1 0.0 3 0 1 236 1 0.0 3 0 0 1 253 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 1,163 Reads with adapters: 22 (1.9%) Reads written (passing filters): 1,163 (100.0%) Total basepairs processed: 127,104 bp Total written (filtered): 127,031 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 22 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.2% C: 18.2% G: 4.5% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19 18.2 0 19 4 2 4.5 0 2 8 1 0.0 0 1