This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.69 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 345,292 (13.0%) Reads that were too short: 12,014 (0.5%) Reads written (passing filters): 333,278 (12.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 69,464,932 bp (8.7%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 345292 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 60 649.0 0 60 7 50 649.0 0 50 8 48 649.0 0 48 9 78 649.0 0 78 10 42 649.0 0 42 11 51 649.0 0 51 12 48 649.0 0 48 13 45 649.0 0 45 14 52 649.0 0 52 15 47 649.0 0 47 16 35 649.0 0 35 17 48 649.0 0 48 18 82 649.0 0 82 19 75 649.0 0 75 20 92 649.0 0 92 21 56 649.0 0 56 22 92 649.0 0 92 23 77 649.0 0 77 24 70 649.0 0 70 25 49 649.0 0 49 26 52 649.0 0 52 27 60 649.0 0 60 28 42 649.0 0 42 29 515 649.0 0 515 30 125 649.0 0 125 31 61 649.0 0 61 32 68 649.0 0 68 33 68 649.0 0 68 34 67 649.0 0 67 35 68 649.0 0 68 36 113 649.0 0 113 37 110 649.0 0 110 38 189 649.0 0 189 39 128 649.0 0 128 40 108 649.0 0 108 41 68 649.0 0 68 42 114 649.0 0 114 43 74 649.0 0 74 44 134 649.0 0 134 45 172 649.0 0 172 46 284 649.0 0 284 47 478 649.0 0 478 48 718 649.0 0 718 49 1306 649.0 0 1306 50 353 649.0 0 353 51 434 649.0 0 434 52 754 649.0 0 754 53 404 649.0 0 404 54 406 649.0 0 406 55 695 649.0 0 695 56 755 649.0 0 755 57 872 649.0 0 872 58 801 649.0 0 801 59 1690 649.0 0 1690 60 1700 649.0 0 1700 61 3845 649.0 0 3845 62 84441 649.0 0 84441 63 52063 649.0 0 52063 64 55724 649.0 0 55724 65 666 649.0 0 666 66 212 649.0 0 212 67 79 649.0 0 79 68 95 649.0 0 95 69 87 649.0 0 87 70 113 649.0 0 113 71 316 649.0 0 316 72 253 649.0 0 253 73 315 649.0 0 315 74 286 649.0 0 286 75 442 649.0 0 442 76 6212 649.0 0 6212 77 6045 649.0 0 6045 78 2654 649.0 0 2654 79 1318 649.0 0 1318 80 105 649.0 0 105 81 130 649.0 0 130 82 59 649.0 0 59 83 51 649.0 0 51 84 53 649.0 0 53 85 72 649.0 0 72 86 64 649.0 0 64 87 57 649.0 0 57 88 59 649.0 0 59 89 46 649.0 0 46 90 63 649.0 0 63 91 81 649.0 0 81 92 63 649.0 0 63 93 110 649.0 0 110 94 81 649.0 0 81 95 81 649.0 0 81 96 65 649.0 0 65 97 54 649.0 0 54 98 116 649.0 0 116 99 712 649.0 0 712 100 362 649.0 0 362 101 57 649.0 0 57 102 67 649.0 0 67 103 70 649.0 0 70 104 105 649.0 0 105 105 66 649.0 0 66 106 79 649.0 0 79 107 234 649.0 0 234 108 243 649.0 0 243 109 249 649.0 0 249 110 93 649.0 0 93 111 85 649.0 0 85 112 91 649.0 0 91 113 64 649.0 0 64 114 43 649.0 0 43 115 96 649.0 0 96 116 70 649.0 0 70 117 72 649.0 0 72 118 78 649.0 0 78 119 80 649.0 0 80 120 83 649.0 0 83 121 67 649.0 0 67 122 48 649.0 0 48 123 65 649.0 0 65 124 105 649.0 0 105 125 84 649.0 0 84 126 149 649.0 0 149 127 158 649.0 0 158 128 150 649.0 0 150 129 224 649.0 0 224 130 122 649.0 0 122 131 85 649.0 0 85 132 108 649.0 0 108 133 75 649.0 0 75 134 95 649.0 0 95 135 78 649.0 0 78 136 71 649.0 0 71 137 186 649.0 0 186 138 145 649.0 0 145 139 122 649.0 0 122 140 143 649.0 0 143 141 158 649.0 0 158 142 361 649.0 0 361 143 533 649.0 0 533 144 5084 649.0 0 5084 145 6101 649.0 0 6101 146 5110 649.0 0 5110 147 228 649.0 0 228 148 160 649.0 0 160 149 217 649.0 0 217 150 122 649.0 0 122 151 174 649.0 0 174 152 261 649.0 0 261 153 156 649.0 0 156 154 227 649.0 0 227 155 194 649.0 0 194 156 1148 649.0 0 1148 157 431 649.0 0 431 158 638 649.0 0 638 159 316 649.0 0 316 160 557 649.0 0 557 161 443 649.0 0 443 162 801 649.0 0 801 163 1367 649.0 0 1367 164 20154 649.0 0 20154 165 27431 649.0 0 27431 166 13843 649.0 0 13843 167 611 649.0 0 611 168 104 649.0 0 104 169 368 649.0 0 368 170 476 649.0 0 476 171 606 649.0 0 606 172 582 649.0 0 582 173 301 649.0 0 301 174 272 649.0 0 272 175 2020 649.0 0 2020 176 80 649.0 0 80 177 70 649.0 0 70 178 104 649.0 0 104 179 254 649.0 0 254 180 195 649.0 0 195 181 212 649.0 0 212 182 178 649.0 0 178 183 107 649.0 0 107 184 256 649.0 0 256 185 202 649.0 0 202 186 168 649.0 0 168 187 63 649.0 0 63 188 101 649.0 0 101 189 468 649.0 0 468 190 598 649.0 0 598 191 547 649.0 0 547 192 432 649.0 0 432 193 53 649.0 0 53 194 43 649.0 0 43 195 35 649.0 0 35 196 46 649.0 0 46 197 64 649.0 0 64 198 109 649.0 0 109 199 75 649.0 0 75 200 70 649.0 0 70 201 55 649.0 0 55 202 74 649.0 0 74 203 79 649.0 0 79 204 72 649.0 0 72 205 72 649.0 0 72 206 68 649.0 0 68 207 53 649.0 0 53 208 82 649.0 0 82 209 107 649.0 0 107 210 148 649.0 0 148 211 94 649.0 0 94 212 68 649.0 0 68 213 76 649.0 0 76 214 74 649.0 0 74 215 111 649.0 0 111 216 134 649.0 0 134 217 158 649.0 0 158 218 141 649.0 0 141 219 75 649.0 0 75 220 64 649.0 0 64 221 53 649.0 0 53 222 52 649.0 0 52 223 58 649.0 0 58 224 93 649.0 0 93 225 129 649.0 0 129 226 289 649.0 0 289 227 305 649.0 0 305 228 198 649.0 0 198 229 548 649.0 0 548 230 289 649.0 0 289 231 224 649.0 0 224 232 77 649.0 0 77 233 50 649.0 0 50 234 53 649.0 0 53 235 66 649.0 0 66 236 67 649.0 0 67 237 74 649.0 0 74 238 74 649.0 0 74 239 77 649.0 0 77 240 64 649.0 0 64 241 68 649.0 0 68 242 42 649.0 0 42 243 81 649.0 0 81 244 65 649.0 0 65 245 86 649.0 0 86 246 79 649.0 0 79 247 90 649.0 0 90 248 139 649.0 0 139 249 70 649.0 0 70 250 59 649.0 0 59 251 56 649.0 0 56 252 77 649.0 0 77 253 60 649.0 0 60 254 72 649.0 0 72 255 73 649.0 0 73 256 64 649.0 0 64 257 64 649.0 0 64 258 69 649.0 0 69 259 99 649.0 0 99 260 158 649.0 0 158 261 142 649.0 0 142 262 168 649.0 0 168 263 80 649.0 0 80 264 88 649.0 0 88 265 113 649.0 0 113 266 156 649.0 0 156 267 168 649.0 0 168 268 104 649.0 0 104 269 74 649.0 0 74 270 104 649.0 0 104 271 210 649.0 0 210 272 155 649.0 0 155 273 129 649.0 0 129 274 69 649.0 0 69 275 80 649.0 0 80 276 76 649.0 0 76 277 247 649.0 0 247 278 109 649.0 0 109 279 243 649.0 0 243 280 86 649.0 0 86 281 115 649.0 0 115 282 80 649.0 0 80 283 72 649.0 0 72 284 70 649.0 0 70 285 125 649.0 0 125 286 229 649.0 0 229 287 526 649.0 0 526 288 389 649.0 0 389 289 277 649.0 0 277 290 120 649.0 0 120 291 84 649.0 0 84 292 131 649.0 0 131 293 123 649.0 0 123 294 142 649.0 0 142 295 106 649.0 0 106 296 82 649.0 0 82 297 220 649.0 0 220 298 69 649.0 0 69 299 123 649.0 0 123 300 74 649.0 0 74 301 95 649.0 0 95 Finished in 11.51 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 333,278 Reads with adapters: 671 (0.2%) Reads that were too short: 150 (0.0%) Reads written (passing filters): 521 (0.2%) Total basepairs processed: 69,464,932 bp Total written (filtered): 62,054 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 671 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 14 0.0 3 13 0 0 1 20 1 0.0 3 0 0 0 1 21 7 0.0 3 0 0 0 7 27 1 0.0 3 0 0 0 1 28 27 0.0 3 0 0 0 27 29 9 0.0 3 0 0 0 9 33 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 1 0 0 1 50 1 0.0 3 0 0 0 1 51 8 0.0 3 2 0 0 6 52 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 56 12 0.0 3 0 0 10 2 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 104 5 0.0 3 0 0 0 5 107 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 109 12 0.0 3 0 0 0 12 110 1 0.0 3 0 1 111 1 0.0 3 0 0 0 1 112 4 0.0 3 0 0 0 4 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 10 0.0 3 0 0 0 10 124 5 0.0 3 0 0 1 4 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 10 0.0 3 0 0 0 10 129 297 0.0 3 0 0 2 295 130 30 0.0 3 0 0 1 29 131 2 0.0 3 0 0 0 2 132 35 0.0 3 0 0 0 35 133 9 0.0 3 0 0 0 9 134 1 0.0 3 0 0 0 1 135 1 0.0 3 1 138 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 1 0.0 3 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 1 152 1 0.0 3 0 0 1 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 4 0.0 3 0 0 0 4 177 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 11 0.0 3 0 0 0 11 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 212 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 219 6 0.0 3 0 0 0 6 221 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 1 230 7 0.0 3 0 0 0 7 231 37 0.0 3 0 0 2 35 232 2 0.0 3 0 0 0 2 236 3 0.0 3 0 0 0 3 237 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 241 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (19 us/read; 3.13 M reads/minute). === Summary === Total reads processed: 521 Reads with adapters: 0 (0.0%) Reads written (passing filters): 521 (100.0%) Total basepairs processed: 62,054 bp Total written (filtered): 62,054 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.