This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.50 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 80,549 (3.6%) Reads that were too short: 10,433 (0.5%) Reads written (passing filters): 70,116 (3.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 11,563,422 bp (1.7%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 80549 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 421 546.6 0 421 7 4122 546.6 0 4122 8 3411 546.6 0 3411 9 50 546.6 0 50 10 50 546.6 0 50 11 44 546.6 0 44 12 96 546.6 0 96 13 30 546.6 0 30 14 44 546.6 0 44 15 49 546.6 0 49 16 54 546.6 0 54 17 54 546.6 0 54 18 71 546.6 0 71 19 102 546.6 0 102 20 89 546.6 0 89 21 111 546.6 0 111 22 45 546.6 0 45 23 43 546.6 0 43 24 45 546.6 0 45 25 33 546.6 0 33 26 49 546.6 0 49 27 53 546.6 0 53 28 40 546.6 0 40 29 54 546.6 0 54 30 58 546.6 0 58 31 45 546.6 0 45 32 41 546.6 0 41 33 40 546.6 0 40 34 53 546.6 0 53 35 65 546.6 0 65 36 63 546.6 0 63 37 37 546.6 0 37 38 204 546.6 0 204 39 192 546.6 0 192 40 208 546.6 0 208 41 108 546.6 0 108 42 47 546.6 0 47 43 40 546.6 0 40 44 47 546.6 0 47 45 55 546.6 0 55 46 49 546.6 0 49 47 70 546.6 0 70 48 51 546.6 0 51 49 48 546.6 0 48 50 46 546.6 0 46 51 47 546.6 0 47 52 43 546.6 0 43 53 42 546.6 0 42 54 52 546.6 0 52 55 35 546.6 0 35 56 51 546.6 0 51 57 56 546.6 0 56 58 61 546.6 0 61 59 47 546.6 0 47 60 44 546.6 0 44 61 37 546.6 0 37 62 37 546.6 0 37 63 36 546.6 0 36 64 48 546.6 0 48 65 35 546.6 0 35 66 51 546.6 0 51 67 45 546.6 0 45 68 42 546.6 0 42 69 40 546.6 0 40 70 59 546.6 0 59 71 54 546.6 0 54 72 53 546.6 0 53 73 137 546.6 0 137 74 56 546.6 0 56 75 65 546.6 0 65 76 260 546.6 0 260 77 131 546.6 0 131 78 163 546.6 0 163 79 78 546.6 0 78 80 49 546.6 0 49 81 60 546.6 0 60 82 50 546.6 0 50 83 48 546.6 0 48 84 68 546.6 0 68 85 58 546.6 0 58 86 55 546.6 0 55 87 69 546.6 0 69 88 48 546.6 0 48 89 56 546.6 0 56 90 41 546.6 0 41 91 80 546.6 0 80 92 81 546.6 0 81 93 259 546.6 0 259 94 438 546.6 0 438 95 590 546.6 0 590 96 595 546.6 0 595 97 193 546.6 0 193 98 728 546.6 0 728 99 73 546.6 0 73 100 40 546.6 0 40 101 38 546.6 0 38 102 42 546.6 0 42 103 40 546.6 0 40 104 38 546.6 0 38 105 34 546.6 0 34 106 56 546.6 0 56 107 60 546.6 0 60 108 58 546.6 0 58 109 42 546.6 0 42 110 52 546.6 0 52 111 46 546.6 0 46 112 52 546.6 0 52 113 46 546.6 0 46 114 87 546.6 0 87 115 60 546.6 0 60 116 67 546.6 0 67 117 48 546.6 0 48 118 43 546.6 0 43 119 32 546.6 0 32 120 31 546.6 0 31 121 31 546.6 0 31 122 23 546.6 0 23 123 36 546.6 0 36 124 37 546.6 0 37 125 52 546.6 0 52 126 41 546.6 0 41 127 42 546.6 0 42 128 71 546.6 0 71 129 148 546.6 0 148 130 424 546.6 0 424 131 268 546.6 0 268 132 1701 546.6 0 1701 133 62 546.6 0 62 134 74 546.6 0 74 135 43 546.6 0 43 136 115 546.6 0 115 137 74 546.6 0 74 138 116 546.6 0 116 139 35 546.6 0 35 140 52 546.6 0 52 141 48 546.6 0 48 142 139 546.6 0 139 143 148 546.6 0 148 144 230 546.6 0 230 145 160 546.6 0 160 146 50 546.6 0 50 147 80 546.6 0 80 148 44 546.6 0 44 149 44 546.6 0 44 150 42 546.6 0 42 151 44 546.6 0 44 152 78 546.6 0 78 153 41 546.6 0 41 154 58 546.6 0 58 155 66 546.6 0 66 156 62 546.6 0 62 157 90 546.6 0 90 158 81 546.6 0 81 159 43 546.6 0 43 160 44 546.6 0 44 161 64 546.6 0 64 162 89 546.6 0 89 163 86 546.6 0 86 164 125 546.6 0 125 165 216 546.6 0 216 166 381 546.6 0 381 167 544 546.6 0 544 168 1475 546.6 0 1475 169 28998 546.6 0 28998 170 1175 546.6 0 1175 171 12739 546.6 0 12739 172 430 546.6 0 430 173 51 546.6 0 51 174 112 546.6 0 112 175 29 546.6 0 29 176 43 546.6 0 43 177 47 546.6 0 47 178 128 546.6 0 128 179 40 546.6 0 40 180 42 546.6 0 42 181 25 546.6 0 25 182 41 546.6 0 41 183 46 546.6 0 46 184 71 546.6 0 71 185 58 546.6 0 58 186 47 546.6 0 47 187 46 546.6 0 46 188 37 546.6 0 37 189 42 546.6 0 42 190 38 546.6 0 38 191 28 546.6 0 28 192 55 546.6 0 55 193 43 546.6 0 43 194 90 546.6 0 90 195 40 546.6 0 40 196 38 546.6 0 38 197 43 546.6 0 43 198 48 546.6 0 48 199 47 546.6 0 47 200 52 546.6 0 52 201 47 546.6 0 47 202 70 546.6 0 70 203 155 546.6 0 155 204 109 546.6 0 109 205 69 546.6 0 69 206 118 546.6 0 118 207 35 546.6 0 35 208 50 546.6 0 50 209 94 546.6 0 94 210 54 546.6 0 54 211 70 546.6 0 70 212 61 546.6 0 61 213 46 546.6 0 46 214 95 546.6 0 95 215 60 546.6 0 60 216 45 546.6 0 45 217 87 546.6 0 87 218 48 546.6 0 48 219 41 546.6 0 41 220 38 546.6 0 38 221 96 546.6 0 96 222 253 546.6 0 253 223 173 546.6 0 173 224 196 546.6 0 196 225 30 546.6 0 30 226 58 546.6 0 58 227 37 546.6 0 37 228 58 546.6 0 58 229 32 546.6 0 32 230 91 546.6 0 91 231 74 546.6 0 74 232 111 546.6 0 111 233 79 546.6 0 79 234 86 546.6 0 86 235 176 546.6 0 176 236 191 546.6 0 191 237 429 546.6 0 429 238 233 546.6 0 233 239 47 546.6 0 47 240 50 546.6 0 50 241 58 546.6 0 58 242 92 546.6 0 92 243 83 546.6 0 83 244 54 546.6 0 54 245 50 546.6 0 50 246 49 546.6 0 49 247 42 546.6 0 42 248 28 546.6 0 28 249 40 546.6 0 40 250 44 546.6 0 44 251 57 546.6 0 57 252 50 546.6 0 50 253 59 546.6 0 59 254 87 546.6 0 87 255 79 546.6 0 79 256 80 546.6 0 80 257 82 546.6 0 82 258 60 546.6 0 60 259 39 546.6 0 39 260 64 546.6 0 64 261 84 546.6 0 84 262 146 546.6 0 146 263 218 546.6 0 218 264 83 546.6 0 83 265 57 546.6 0 57 266 55 546.6 0 55 267 51 546.6 0 51 268 57 546.6 0 57 269 57 546.6 0 57 270 83 546.6 0 83 271 178 546.6 0 178 272 426 546.6 0 426 273 577 546.6 0 577 274 434 546.6 0 434 275 68 546.6 0 68 276 57 546.6 0 57 277 51 546.6 0 51 278 67 546.6 0 67 279 81 546.6 0 81 280 58 546.6 0 58 281 78 546.6 0 78 282 77 546.6 0 77 283 72 546.6 0 72 284 66 546.6 0 66 285 57 546.6 0 57 286 61 546.6 0 61 287 57 546.6 0 57 288 54 546.6 0 54 289 63 546.6 0 63 290 74 546.6 0 74 291 60 546.6 0 60 292 99 546.6 0 99 293 84 546.6 0 84 294 125 546.6 0 125 295 73 546.6 0 73 296 310 546.6 0 310 297 513 546.6 0 513 298 338 546.6 0 338 299 116 546.6 0 116 300 101 546.6 0 101 301 25 546.6 0 25 Finished in 2.54 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 70,116 Reads with adapters: 3,998 (5.7%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 3,981 (5.7%) Total basepairs processed: 11,563,422 bp Total written (filtered): 1,067,164 bp (9.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3998 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 19 42 0.0 3 0 33 6 3 20 3534 0.0 4 3334 154 35 5 6 21 17 0.0 4 4 9 2 2 22 8 0.0 4 0 3 4 1 23 184 0.0 4 162 8 5 6 3 26 3 0.0 4 0 0 0 1 2 33 1 0.0 4 0 0 0 0 1 41 56 0.0 4 0 0 0 0 56 42 3 0.0 4 0 0 0 0 3 43 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 75 4 0.0 4 4 76 1 0.0 4 0 0 1 77 2 0.0 4 2 78 5 0.0 4 3 2 82 3 0.0 4 0 0 0 0 3 86 1 0.0 4 0 1 87 2 0.0 4 2 88 3 0.0 4 1 1 0 0 1 89 3 0.0 4 3 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 102 0.0 4 0 0 0 0 102 162 1 0.0 4 0 0 0 0 1 184 3 0.0 4 0 0 0 0 3 197 2 0.0 4 0 0 0 0 2 198 2 0.0 4 0 0 0 0 2 199 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 3,981 Reads with adapters: 1,649 (41.4%) Reads written (passing filters): 3,981 (100.0%) Total basepairs processed: 1,067,164 bp Total written (filtered): 757,912 bp (71.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1649 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 25.9% C: 46.6% G: 26.7% T: 0.7% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 3 62.2 0 3 4 3 15.6 0 3 9 5 0.0 0 0 5 10 73 0.0 1 5 68 11 418 0.0 1 13 405 12 4 0.0 1 0 4 64 1 0.0 1 0 1 177 1 0.0 1 1 209 2 0.0 1 2 257 1 0.0 1 1 259 385 0.0 1 351 34 260 14 0.0 1 14 261 8 0.0 1 8 269 4 0.0 1 3 1 270 664 0.0 1 605 59 271 43 0.0 1 43 272 18 0.0 1 17 1 273 2 0.0 1 2