This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.48 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 69,977 (3.1%) Reads that were too short: 7,730 (0.3%) Reads written (passing filters): 62,247 (2.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,077,924 bp (2.5%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 69977 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5255 546.6 0 5255 7 713 546.6 0 713 8 26311 546.6 0 26311 9 19620 546.6 0 19620 10 59 546.6 0 59 11 215 546.6 0 215 12 20 546.6 0 20 13 24 546.6 0 24 14 17 546.6 0 17 15 32 546.6 0 32 16 16 546.6 0 16 17 7 546.6 0 7 18 15 546.6 0 15 19 17 546.6 0 17 20 20 546.6 0 20 21 15 546.6 0 15 22 23 546.6 0 23 23 19 546.6 0 19 24 10 546.6 0 10 25 18 546.6 0 18 26 8 546.6 0 8 27 20 546.6 0 20 28 12 546.6 0 12 29 9 546.6 0 9 30 13 546.6 0 13 31 8 546.6 0 8 32 8 546.6 0 8 33 18 546.6 0 18 34 20 546.6 0 20 35 41 546.6 0 41 36 16 546.6 0 16 37 25 546.6 0 25 38 38 546.6 0 38 39 15 546.6 0 15 40 19 546.6 0 19 41 27 546.6 0 27 42 25 546.6 0 25 43 28 546.6 0 28 44 15 546.6 0 15 45 22 546.6 0 22 46 25 546.6 0 25 47 189 546.6 0 189 48 285 546.6 0 285 49 324 546.6 0 324 50 920 546.6 0 920 51 53 546.6 0 53 52 13 546.6 0 13 53 12 546.6 0 12 54 13 546.6 0 13 55 13 546.6 0 13 56 10 546.6 0 10 57 10 546.6 0 10 58 14 546.6 0 14 59 12 546.6 0 12 60 19 546.6 0 19 61 15 546.6 0 15 62 12 546.6 0 12 63 12 546.6 0 12 64 12 546.6 0 12 65 4 546.6 0 4 66 18 546.6 0 18 67 18 546.6 0 18 68 11 546.6 0 11 69 19 546.6 0 19 70 18 546.6 0 18 71 5 546.6 0 5 72 13 546.6 0 13 73 14 546.6 0 14 74 30 546.6 0 30 75 13 546.6 0 13 76 36 546.6 0 36 77 14 546.6 0 14 78 15 546.6 0 15 79 24 546.6 0 24 80 27 546.6 0 27 81 47 546.6 0 47 82 200 546.6 0 200 83 527 546.6 0 527 84 29 546.6 0 29 85 17 546.6 0 17 86 11 546.6 0 11 87 46 546.6 0 46 88 17 546.6 0 17 89 27 546.6 0 27 90 46 546.6 0 46 91 21 546.6 0 21 92 12 546.6 0 12 93 19 546.6 0 19 94 22 546.6 0 22 95 19 546.6 0 19 96 22 546.6 0 22 97 99 546.6 0 99 98 319 546.6 0 319 99 18 546.6 0 18 100 16 546.6 0 16 101 13 546.6 0 13 102 10 546.6 0 10 103 9 546.6 0 9 104 21 546.6 0 21 105 19 546.6 0 19 106 11 546.6 0 11 107 18 546.6 0 18 108 17 546.6 0 17 109 18 546.6 0 18 110 17 546.6 0 17 111 19 546.6 0 19 112 14 546.6 0 14 113 30 546.6 0 30 114 44 546.6 0 44 115 57 546.6 0 57 116 22 546.6 0 22 117 24 546.6 0 24 118 67 546.6 0 67 119 79 546.6 0 79 120 246 546.6 0 246 121 23 546.6 0 23 122 20 546.6 0 20 123 16 546.6 0 16 124 17 546.6 0 17 125 17 546.6 0 17 126 30 546.6 0 30 127 22 546.6 0 22 128 25 546.6 0 25 129 29 546.6 0 29 130 25 546.6 0 25 131 11 546.6 0 11 132 23 546.6 0 23 133 14 546.6 0 14 134 26 546.6 0 26 135 37 546.6 0 37 136 80 546.6 0 80 137 35 546.6 0 35 138 72 546.6 0 72 139 18 546.6 0 18 140 21 546.6 0 21 141 21 546.6 0 21 142 38 546.6 0 38 143 35 546.6 0 35 144 45 546.6 0 45 145 32 546.6 0 32 146 104 546.6 0 104 147 40 546.6 0 40 148 93 546.6 0 93 149 13 546.6 0 13 150 30 546.6 0 30 151 27 546.6 0 27 152 60 546.6 0 60 153 76 546.6 0 76 154 85 546.6 0 85 155 24 546.6 0 24 156 22 546.6 0 22 157 20 546.6 0 20 158 33 546.6 0 33 159 26 546.6 0 26 160 20 546.6 0 20 161 7 546.6 0 7 162 20 546.6 0 20 163 9 546.6 0 9 164 13 546.6 0 13 165 19 546.6 0 19 166 26 546.6 0 26 167 10 546.6 0 10 168 19 546.6 0 19 169 15 546.6 0 15 170 24 546.6 0 24 171 18 546.6 0 18 172 34 546.6 0 34 173 18 546.6 0 18 174 4 546.6 0 4 175 13 546.6 0 13 176 24 546.6 0 24 177 16 546.6 0 16 178 27 546.6 0 27 179 19 546.6 0 19 180 30 546.6 0 30 181 61 546.6 0 61 182 45 546.6 0 45 183 73 546.6 0 73 184 16 546.6 0 16 185 19 546.6 0 19 186 19 546.6 0 19 187 19 546.6 0 19 188 28 546.6 0 28 189 42 546.6 0 42 190 39 546.6 0 39 191 99 546.6 0 99 192 144 546.6 0 144 193 147 546.6 0 147 194 159 546.6 0 159 195 857 546.6 0 857 196 519 546.6 0 519 197 676 546.6 0 676 198 86 546.6 0 86 199 43 546.6 0 43 200 15 546.6 0 15 201 27 546.6 0 27 202 19 546.6 0 19 203 22 546.6 0 22 204 16 546.6 0 16 205 17 546.6 0 17 206 26 546.6 0 26 207 18 546.6 0 18 208 17 546.6 0 17 209 27 546.6 0 27 210 24 546.6 0 24 211 17 546.6 0 17 212 28 546.6 0 28 213 35 546.6 0 35 214 102 546.6 0 102 215 89 546.6 0 89 216 81 546.6 0 81 217 34 546.6 0 34 218 28 546.6 0 28 219 29 546.6 0 29 220 31 546.6 0 31 221 35 546.6 0 35 222 36 546.6 0 36 223 28 546.6 0 28 224 27 546.6 0 27 225 28 546.6 0 28 226 29 546.6 0 29 227 18 546.6 0 18 228 18 546.6 0 18 229 35 546.6 0 35 230 32 546.6 0 32 231 81 546.6 0 81 232 49 546.6 0 49 233 39 546.6 0 39 234 70 546.6 0 70 235 103 546.6 0 103 236 147 546.6 0 147 237 41 546.6 0 41 238 62 546.6 0 62 239 96 546.6 0 96 240 114 546.6 0 114 241 109 546.6 0 109 242 30 546.6 0 30 243 132 546.6 0 132 244 128 546.6 0 128 245 311 546.6 0 311 246 82 546.6 0 82 247 77 546.6 0 77 248 72 546.6 0 72 249 147 546.6 0 147 250 76 546.6 0 76 251 89 546.6 0 89 252 106 546.6 0 106 253 139 546.6 0 139 254 91 546.6 0 91 255 33 546.6 0 33 256 35 546.6 0 35 257 55 546.6 0 55 258 46 546.6 0 46 259 90 546.6 0 90 260 90 546.6 0 90 261 181 546.6 0 181 262 240 546.6 0 240 263 266 546.6 0 266 264 60 546.6 0 60 265 55 546.6 0 55 266 52 546.6 0 52 267 32 546.6 0 32 268 67 546.6 0 67 269 125 546.6 0 125 270 162 546.6 0 162 271 128 546.6 0 128 272 54 546.6 0 54 273 35 546.6 0 35 274 45 546.6 0 45 275 44 546.6 0 44 276 42 546.6 0 42 277 25 546.6 0 25 278 43 546.6 0 43 279 42 546.6 0 42 280 30 546.6 0 30 281 57 546.6 0 57 282 52 546.6 0 52 283 70 546.6 0 70 284 81 546.6 0 81 285 125 546.6 0 125 286 152 546.6 0 152 287 61 546.6 0 61 288 220 546.6 0 220 289 267 546.6 0 267 290 112 546.6 0 112 291 178 546.6 0 178 292 90 546.6 0 90 293 118 546.6 0 118 294 56 546.6 0 56 295 52 546.6 0 52 296 58 546.6 0 58 297 104 546.6 0 104 298 142 546.6 0 142 299 166 546.6 0 166 300 82 546.6 0 82 301 73 546.6 0 73 Finished in 3.24 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 62,247 Reads with adapters: 22,300 (35.8%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 22,280 (35.8%) Total basepairs processed: 17,077,924 bp Total written (filtered): 6,016,048 bp (35.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22300 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 18 0.0 3 0 0 13 2 3 19 379 0.0 3 0 325 21 26 7 20 21244 0.0 4 19820 1155 184 67 18 21 55 0.0 4 8 38 6 3 22 15 0.0 4 6 1 5 1 2 23 119 0.0 4 1 0 0 93 25 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 86 9 0.0 4 0 0 0 0 9 115 1 0.0 4 0 0 0 0 1 125 5 0.0 4 0 0 0 0 5 126 6 0.0 4 0 0 0 0 6 127 375 0.0 4 0 0 0 0 375 128 1 0.0 4 0 0 0 0 1 130 43 0.0 4 0 0 0 0 43 153 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 3 0.0 4 0 0 0 1 2 231 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 22,280 Reads with adapters: 4,723 (21.2%) Reads written (passing filters): 22,280 (100.0%) Total basepairs processed: 6,016,048 bp Total written (filtered): 5,963,599 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4723 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 8.0% G: 87.1% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 348.1 0 8 4 5 87.0 0 5 8 1 0.3 0 1 9 463 0.1 0 45 418 10 3585 0.0 1 254 3331 11 598 0.0 1 31 567 12 14 0.0 1 1 13 13 19 0.0 1 0 19 150 12 0.0 1 9 3 169 4 0.0 1 3 1 177 2 0.0 1 0 2 208 11 0.0 1 9 2 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.