This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.69 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 61,003 (2.7%) Reads that were too short: 3,726 (0.2%) Reads written (passing filters): 57,277 (2.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 16,210,214 bp (2.4%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 61003 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4406 546.6 0 4406 7 645 546.6 0 645 8 23544 546.6 0 23544 9 24061 546.6 0 24061 10 49 546.6 0 49 11 32 546.6 0 32 12 12 546.6 0 12 13 13 546.6 0 13 14 3 546.6 0 3 15 12 546.6 0 12 16 7 546.6 0 7 17 3 546.6 0 3 18 8 546.6 0 8 19 6 546.6 0 6 20 6 546.6 0 6 21 8 546.6 0 8 22 12 546.6 0 12 23 7 546.6 0 7 24 8 546.6 0 8 25 4 546.6 0 4 26 3 546.6 0 3 27 10 546.6 0 10 28 6 546.6 0 6 29 8 546.6 0 8 30 7 546.6 0 7 31 8 546.6 0 8 32 5 546.6 0 5 33 10 546.6 0 10 34 6 546.6 0 6 35 7 546.6 0 7 36 6 546.6 0 6 37 6 546.6 0 6 38 14 546.6 0 14 39 6 546.6 0 6 40 8 546.6 0 8 41 8 546.6 0 8 42 15 546.6 0 15 43 13 546.6 0 13 44 7 546.6 0 7 45 10 546.6 0 10 46 9 546.6 0 9 47 16 546.6 0 16 48 80 546.6 0 80 49 145 546.6 0 145 50 324 546.6 0 324 51 20 546.6 0 20 52 8 546.6 0 8 53 4 546.6 0 4 54 10 546.6 0 10 55 4 546.6 0 4 56 4 546.6 0 4 57 5 546.6 0 5 58 12 546.6 0 12 59 5 546.6 0 5 60 5 546.6 0 5 61 6 546.6 0 6 62 10 546.6 0 10 63 5 546.6 0 5 64 13 546.6 0 13 65 5 546.6 0 5 66 2 546.6 0 2 67 19 546.6 0 19 68 5 546.6 0 5 69 10 546.6 0 10 70 1 546.6 0 1 71 8 546.6 0 8 72 5 546.6 0 5 73 14 546.6 0 14 74 12 546.6 0 12 75 10 546.6 0 10 76 21 546.6 0 21 77 8 546.6 0 8 78 8 546.6 0 8 79 3 546.6 0 3 80 12 546.6 0 12 81 7 546.6 0 7 82 52 546.6 0 52 83 84 546.6 0 84 84 14 546.6 0 14 85 12 546.6 0 12 86 13 546.6 0 13 87 5 546.6 0 5 88 5 546.6 0 5 89 10 546.6 0 10 90 15 546.6 0 15 91 6 546.6 0 6 92 11 546.6 0 11 93 7 546.6 0 7 94 8 546.6 0 8 95 12 546.6 0 12 96 13 546.6 0 13 97 39 546.6 0 39 98 164 546.6 0 164 99 10 546.6 0 10 100 11 546.6 0 11 101 4 546.6 0 4 102 6 546.6 0 6 103 6 546.6 0 6 104 8 546.6 0 8 105 12 546.6 0 12 106 7 546.6 0 7 107 16 546.6 0 16 108 12 546.6 0 12 109 14 546.6 0 14 110 8 546.6 0 8 111 11 546.6 0 11 112 13 546.6 0 13 113 9 546.6 0 9 114 27 546.6 0 27 115 13 546.6 0 13 116 16 546.6 0 16 117 9 546.6 0 9 118 26 546.6 0 26 119 36 546.6 0 36 120 103 546.6 0 103 121 16 546.6 0 16 122 13 546.6 0 13 123 5 546.6 0 5 124 3 546.6 0 3 125 5 546.6 0 5 126 15 546.6 0 15 127 17 546.6 0 17 128 12 546.6 0 12 129 35 546.6 0 35 130 9 546.6 0 9 131 12 546.6 0 12 132 14 546.6 0 14 133 6 546.6 0 6 134 9 546.6 0 9 135 12 546.6 0 12 136 23 546.6 0 23 137 14 546.6 0 14 138 25 546.6 0 25 139 12 546.6 0 12 140 4 546.6 0 4 141 14 546.6 0 14 142 23 546.6 0 23 143 11 546.6 0 11 144 11 546.6 0 11 145 16 546.6 0 16 146 87 546.6 0 87 147 17 546.6 0 17 148 78 546.6 0 78 149 9 546.6 0 9 150 17 546.6 0 17 151 7 546.6 0 7 152 65 546.6 0 65 153 69 546.6 0 69 154 85 546.6 0 85 155 12 546.6 0 12 156 6 546.6 0 6 157 9 546.6 0 9 158 9 546.6 0 9 159 8 546.6 0 8 160 11 546.6 0 11 161 12 546.6 0 12 162 17 546.6 0 17 163 6 546.6 0 6 164 9 546.6 0 9 165 12 546.6 0 12 166 7 546.6 0 7 167 11 546.6 0 11 168 16 546.6 0 16 169 3 546.6 0 3 170 12 546.6 0 12 171 6 546.6 0 6 172 14 546.6 0 14 173 8 546.6 0 8 174 11 546.6 0 11 175 14 546.6 0 14 176 13 546.6 0 13 177 8 546.6 0 8 178 15 546.6 0 15 179 12 546.6 0 12 180 22 546.6 0 22 181 73 546.6 0 73 182 16 546.6 0 16 183 58 546.6 0 58 184 9 546.6 0 9 185 9 546.6 0 9 186 8 546.6 0 8 187 7 546.6 0 7 188 16 546.6 0 16 189 18 546.6 0 18 190 15 546.6 0 15 191 86 546.6 0 86 192 132 546.6 0 132 193 140 546.6 0 140 194 49 546.6 0 49 195 354 546.6 0 354 196 214 546.6 0 214 197 257 546.6 0 257 198 26 546.6 0 26 199 10 546.6 0 10 200 15 546.6 0 15 201 21 546.6 0 21 202 12 546.6 0 12 203 8 546.6 0 8 204 11 546.6 0 11 205 6 546.6 0 6 206 6 546.6 0 6 207 4 546.6 0 4 208 12 546.6 0 12 209 13 546.6 0 13 210 9 546.6 0 9 211 15 546.6 0 15 212 14 546.6 0 14 213 9 546.6 0 9 214 22 546.6 0 22 215 26 546.6 0 26 216 21 546.6 0 21 217 8 546.6 0 8 218 22 546.6 0 22 219 6 546.6 0 6 220 10 546.6 0 10 221 13 546.6 0 13 222 14 546.6 0 14 223 13 546.6 0 13 224 12 546.6 0 12 225 9 546.6 0 9 226 9 546.6 0 9 227 14 546.6 0 14 228 5 546.6 0 5 229 6 546.6 0 6 230 14 546.6 0 14 231 29 546.6 0 29 232 22 546.6 0 22 233 18 546.6 0 18 234 44 546.6 0 44 235 101 546.6 0 101 236 143 546.6 0 143 237 25 546.6 0 25 238 16 546.6 0 16 239 33 546.6 0 33 240 32 546.6 0 32 241 23 546.6 0 23 242 12 546.6 0 12 243 32 546.6 0 32 244 49 546.6 0 49 245 88 546.6 0 88 246 27 546.6 0 27 247 26 546.6 0 26 248 22 546.6 0 22 249 69 546.6 0 69 250 32 546.6 0 32 251 59 546.6 0 59 252 36 546.6 0 36 253 60 546.6 0 60 254 23 546.6 0 23 255 11 546.6 0 11 256 17 546.6 0 17 257 24 546.6 0 24 258 26 546.6 0 26 259 45 546.6 0 45 260 57 546.6 0 57 261 65 546.6 0 65 262 109 546.6 0 109 263 111 546.6 0 111 264 13 546.6 0 13 265 14 546.6 0 14 266 9 546.6 0 9 267 10 546.6 0 10 268 21 546.6 0 21 269 26 546.6 0 26 270 47 546.6 0 47 271 28 546.6 0 28 272 13 546.6 0 13 273 11 546.6 0 11 274 48 546.6 0 48 275 21 546.6 0 21 276 8 546.6 0 8 277 10 546.6 0 10 278 9 546.6 0 9 279 14 546.6 0 14 280 19 546.6 0 19 281 17 546.6 0 17 282 26 546.6 0 26 283 39 546.6 0 39 284 65 546.6 0 65 285 46 546.6 0 46 286 66 546.6 0 66 287 41 546.6 0 41 288 340 546.6 0 340 289 267 546.6 0 267 290 84 546.6 0 84 291 82 546.6 0 82 292 54 546.6 0 54 293 102 546.6 0 102 294 22 546.6 0 22 295 26 546.6 0 26 296 38 546.6 0 38 297 30 546.6 0 30 298 57 546.6 0 57 299 78 546.6 0 78 300 66 546.6 0 66 301 30 546.6 0 30 Finished in 2.70 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 57,277 Reads with adapters: 26,670 (46.6%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 26,654 (46.5%) Total basepairs processed: 16,210,214 bp Total written (filtered): 7,348,616 bp (45.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26670 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 5 0.0 3 0 0 5 16 171 0.0 3 0 164 7 17 22560 0.0 3 22087 461 12 18 50 0.0 3 3 40 5 2 19 44 0.0 3 5 23 15 1 20 3769 0.0 3 3665 73 2 29 21 17 0.0 3 4 13 22 4 0.0 3 0 0 3 1 23 1 0.0 3 0 0 0 1 26 1 0.0 3 1 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 1 53 3 0.0 3 0 0 0 3 56 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 10 0.0 3 0 0 0 10 144 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 295 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.94 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 26,654 Reads with adapters: 259 (1.0%) Reads written (passing filters): 26,654 (100.0%) Total basepairs processed: 7,348,616 bp Total written (filtered): 7,347,787 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 259 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.7% C: 7.7% G: 15.1% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 216 416.5 0 216 4 40 104.1 0 40 5 2 26.0 0 2 11 1 0.0 1 0 1