This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.45 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 57,996 (2.6%) Reads that were too short: 24,025 (1.1%) Reads written (passing filters): 33,971 (1.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 6,923,188 bp (1.0%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 57996 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 494 546.6 0 494 7 2178 546.6 0 2178 8 1951 546.6 0 1951 9 55 546.6 0 55 10 118 546.6 0 118 11 28 546.6 0 28 12 38 546.6 0 38 13 33 546.6 0 33 14 58 546.6 0 58 15 27 546.6 0 27 16 30 546.6 0 30 17 54 546.6 0 54 18 67 546.6 0 67 19 72 546.6 0 72 20 32 546.6 0 32 21 34 546.6 0 34 22 19 546.6 0 19 23 43 546.6 0 43 24 29 546.6 0 29 25 41 546.6 0 41 26 43 546.6 0 43 27 25 546.6 0 25 28 33 546.6 0 33 29 41 546.6 0 41 30 36 546.6 0 36 31 36 546.6 0 36 32 25 546.6 0 25 33 33 546.6 0 33 34 22 546.6 0 22 35 36 546.6 0 36 36 25 546.6 0 25 37 21 546.6 0 21 38 27 546.6 0 27 39 40 546.6 0 40 40 32 546.6 0 32 41 34 546.6 0 34 42 38 546.6 0 38 43 43 546.6 0 43 44 38 546.6 0 38 45 29 546.6 0 29 46 57 546.6 0 57 47 47 546.6 0 47 48 82 546.6 0 82 49 76 546.6 0 76 50 38 546.6 0 38 51 39 546.6 0 39 52 44 546.6 0 44 53 76 546.6 0 76 54 74 546.6 0 74 55 35 546.6 0 35 56 42 546.6 0 42 57 38 546.6 0 38 58 31 546.6 0 31 59 45 546.6 0 45 60 88 546.6 0 88 61 62 546.6 0 62 62 107 546.6 0 107 63 78 546.6 0 78 64 76 546.6 0 76 65 54 546.6 0 54 66 55 546.6 0 55 67 77 546.6 0 77 68 58 546.6 0 58 69 62 546.6 0 62 70 119 546.6 0 119 71 89 546.6 0 89 72 93 546.6 0 93 73 418 546.6 0 418 74 640 546.6 0 640 75 1162 546.6 0 1162 76 262 546.6 0 262 77 3133 546.6 0 3133 78 2063 546.6 0 2063 79 2789 546.6 0 2789 80 36 546.6 0 36 81 33 546.6 0 33 82 33 546.6 0 33 83 23 546.6 0 23 84 40 546.6 0 40 85 42 546.6 0 42 86 65 546.6 0 65 87 31 546.6 0 31 88 39 546.6 0 39 89 41 546.6 0 41 90 43 546.6 0 43 91 33 546.6 0 33 92 44 546.6 0 44 93 39 546.6 0 39 94 46 546.6 0 46 95 37 546.6 0 37 96 38 546.6 0 38 97 54 546.6 0 54 98 26 546.6 0 26 99 60 546.6 0 60 100 28 546.6 0 28 101 34 546.6 0 34 102 32 546.6 0 32 103 28 546.6 0 28 104 38 546.6 0 38 105 69 546.6 0 69 106 286 546.6 0 286 107 101 546.6 0 101 108 643 546.6 0 643 109 37 546.6 0 37 110 38 546.6 0 38 111 27 546.6 0 27 112 73 546.6 0 73 113 43 546.6 0 43 114 49 546.6 0 49 115 73 546.6 0 73 116 56 546.6 0 56 117 59 546.6 0 59 118 51 546.6 0 51 119 37 546.6 0 37 120 38 546.6 0 38 121 54 546.6 0 54 122 40 546.6 0 40 123 51 546.6 0 51 124 32 546.6 0 32 125 61 546.6 0 61 126 66 546.6 0 66 127 62 546.6 0 62 128 125 546.6 0 125 129 746 546.6 0 746 130 301 546.6 0 301 131 75 546.6 0 75 132 80 546.6 0 80 133 190 546.6 0 190 134 224 546.6 0 224 135 255 546.6 0 255 136 177 546.6 0 177 137 65 546.6 0 65 138 67 546.6 0 67 139 56 546.6 0 56 140 111 546.6 0 111 141 68 546.6 0 68 142 75 546.6 0 75 143 58 546.6 0 58 144 88 546.6 0 88 145 180 546.6 0 180 146 735 546.6 0 735 147 1602 546.6 0 1602 148 1799 546.6 0 1799 149 58 546.6 0 58 150 46 546.6 0 46 151 56 546.6 0 56 152 45 546.6 0 45 153 50 546.6 0 50 154 61 546.6 0 61 155 235 546.6 0 235 156 197 546.6 0 197 157 191 546.6 0 191 158 98 546.6 0 98 159 61 546.6 0 61 160 69 546.6 0 69 161 77 546.6 0 77 162 40 546.6 0 40 163 43 546.6 0 43 164 98 546.6 0 98 165 134 546.6 0 134 166 197 546.6 0 197 167 113 546.6 0 113 168 150 546.6 0 150 169 92 546.6 0 92 170 62 546.6 0 62 171 37 546.6 0 37 172 31 546.6 0 31 173 48 546.6 0 48 174 54 546.6 0 54 175 34 546.6 0 34 176 25 546.6 0 25 177 49 546.6 0 49 178 44 546.6 0 44 179 60 546.6 0 60 180 69 546.6 0 69 181 61 546.6 0 61 182 62 546.6 0 62 183 44 546.6 0 44 184 279 546.6 0 279 185 334 546.6 0 334 186 577 546.6 0 577 187 115 546.6 0 115 188 61 546.6 0 61 189 40 546.6 0 40 190 42 546.6 0 42 191 49 546.6 0 49 192 60 546.6 0 60 193 46 546.6 0 46 194 58 546.6 0 58 195 179 546.6 0 179 196 181 546.6 0 181 197 292 546.6 0 292 198 134 546.6 0 134 199 199 546.6 0 199 200 116 546.6 0 116 201 65 546.6 0 65 202 42 546.6 0 42 203 53 546.6 0 53 204 168 546.6 0 168 205 60 546.6 0 60 206 90 546.6 0 90 207 55 546.6 0 55 208 70 546.6 0 70 209 56 546.6 0 56 210 50 546.6 0 50 211 84 546.6 0 84 212 151 546.6 0 151 213 60 546.6 0 60 214 62 546.6 0 62 215 94 546.6 0 94 216 133 546.6 0 133 217 182 546.6 0 182 218 82 546.6 0 82 219 97 546.6 0 97 220 73 546.6 0 73 221 93 546.6 0 93 222 56 546.6 0 56 223 46 546.6 0 46 224 69 546.6 0 69 225 104 546.6 0 104 226 105 546.6 0 105 227 90 546.6 0 90 228 58 546.6 0 58 229 65 546.6 0 65 230 54 546.6 0 54 231 61 546.6 0 61 232 75 546.6 0 75 233 111 546.6 0 111 234 174 546.6 0 174 235 372 546.6 0 372 236 3103 546.6 0 3103 237 1271 546.6 0 1271 238 3749 546.6 0 3749 239 153 546.6 0 153 240 74 546.6 0 74 241 106 546.6 0 106 242 74 546.6 0 74 243 49 546.6 0 49 244 67 546.6 0 67 245 78 546.6 0 78 246 228 546.6 0 228 247 374 546.6 0 374 248 401 546.6 0 401 249 189 546.6 0 189 250 184 546.6 0 184 251 493 546.6 0 493 252 440 546.6 0 440 253 310 546.6 0 310 254 161 546.6 0 161 255 243 546.6 0 243 256 128 546.6 0 128 257 105 546.6 0 105 258 85 546.6 0 85 259 93 546.6 0 93 260 60 546.6 0 60 261 68 546.6 0 68 262 48 546.6 0 48 263 69 546.6 0 69 264 75 546.6 0 75 265 86 546.6 0 86 266 78 546.6 0 78 267 80 546.6 0 80 268 70 546.6 0 70 269 68 546.6 0 68 270 83 546.6 0 83 271 93 546.6 0 93 272 101 546.6 0 101 273 113 546.6 0 113 274 119 546.6 0 119 275 84 546.6 0 84 276 64 546.6 0 64 277 90 546.6 0 90 278 215 546.6 0 215 279 147 546.6 0 147 280 382 546.6 0 382 281 350 546.6 0 350 282 275 546.6 0 275 283 259 546.6 0 259 284 321 546.6 0 321 285 229 546.6 0 229 286 329 546.6 0 329 287 152 546.6 0 152 288 127 546.6 0 127 289 136 546.6 0 136 290 105 546.6 0 105 291 121 546.6 0 121 292 94 546.6 0 94 293 163 546.6 0 163 294 198 546.6 0 198 295 412 546.6 0 412 296 1054 546.6 0 1054 297 808 546.6 0 808 298 662 546.6 0 662 299 238 546.6 0 238 300 132 546.6 0 132 301 314 546.6 0 314 Finished in 1.45 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 33,971 Reads with adapters: 2,413 (7.1%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 2,390 (7.0%) Total basepairs processed: 6,923,188 bp Total written (filtered): 642,348 bp (9.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2413 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 26 0.0 3 0 19 4 3 20 1909 0.0 4 1765 114 17 10 3 21 14 0.0 4 1 11 1 0 1 22 180 0.0 4 169 9 0 1 1 23 164 0.0 4 145 8 4 5 2 24 1 0.0 4 1 26 4 0.0 4 0 0 0 3 1 30 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 24 0.0 4 0 0 0 0 24 59 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 61 15 0.0 4 0 0 0 0 15 68 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 83 4 0.0 4 0 0 0 0 4 84 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 127 40 0.0 4 0 0 0 0 40 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 2,390 Reads with adapters: 370 (15.5%) Reads written (passing filters): 2,390 (100.0%) Total basepairs processed: 642,348 bp Total written (filtered): 638,090 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 370 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.3% C: 8.4% G: 84.6% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 37.3 0 9 9 11 0.0 0 1 10 10 62 0.0 1 3 59 11 282 0.0 1 14 268 12 3 0.0 1 0 3 15 1 0.0 1 0 1 150 1 0.0 1 0 1 209 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.