This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.01 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 114,410 (5.1%) Reads that were too short: 88,368 (3.9%) Reads written (passing filters): 26,042 (1.2%) Total basepairs processed: 673,916,425 bp Total written (filtered): 4,159,031 bp (0.6%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 114410 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 52 546.6 0 52 7 34 546.6 0 34 8 58 546.6 0 58 9 45 546.6 0 45 10 90 546.6 0 90 11 59 546.6 0 59 12 43 546.6 0 43 13 65 546.6 0 65 14 72 546.6 0 72 15 42 546.6 0 42 16 47 546.6 0 47 17 71 546.6 0 71 18 56 546.6 0 56 19 31 546.6 0 31 20 42 546.6 0 42 21 56 546.6 0 56 22 24 546.6 0 24 23 25 546.6 0 25 24 33 546.6 0 33 25 31 546.6 0 31 26 62 546.6 0 62 27 35 546.6 0 35 28 44 546.6 0 44 29 33 546.6 0 33 30 50 546.6 0 50 31 49 546.6 0 49 32 29 546.6 0 29 33 19 546.6 0 19 34 36 546.6 0 36 35 19 546.6 0 19 36 19 546.6 0 19 37 46 546.6 0 46 38 66 546.6 0 66 39 43 546.6 0 43 40 39 546.6 0 39 41 48 546.6 0 48 42 36 546.6 0 36 43 110 546.6 0 110 44 47 546.6 0 47 45 44 546.6 0 44 46 57 546.6 0 57 47 70 546.6 0 70 48 87 546.6 0 87 49 44 546.6 0 44 50 44 546.6 0 44 51 42 546.6 0 42 52 38 546.6 0 38 53 38 546.6 0 38 54 68 546.6 0 68 55 46 546.6 0 46 56 43 546.6 0 43 57 53 546.6 0 53 58 58 546.6 0 58 59 36 546.6 0 36 60 65 546.6 0 65 61 45 546.6 0 45 62 61 546.6 0 61 63 23 546.6 0 23 64 44 546.6 0 44 65 32 546.6 0 32 66 46 546.6 0 46 67 62 546.6 0 62 68 64 546.6 0 64 69 101 546.6 0 101 70 78 546.6 0 78 71 42 546.6 0 42 72 46 546.6 0 46 73 90 546.6 0 90 74 55 546.6 0 55 75 95 546.6 0 95 76 55 546.6 0 55 77 55 546.6 0 55 78 76 546.6 0 76 79 53 546.6 0 53 80 43 546.6 0 43 81 38 546.6 0 38 82 55 546.6 0 55 83 44 546.6 0 44 84 44 546.6 0 44 85 40 546.6 0 40 86 57 546.6 0 57 87 57 546.6 0 57 88 46 546.6 0 46 89 30 546.6 0 30 90 42 546.6 0 42 91 49 546.6 0 49 92 52 546.6 0 52 93 44 546.6 0 44 94 65 546.6 0 65 95 91 546.6 0 91 96 54 546.6 0 54 97 247 546.6 0 247 98 97 546.6 0 97 99 137 546.6 0 137 100 44 546.6 0 44 101 47 546.6 0 47 102 31 546.6 0 31 103 55 546.6 0 55 104 60 546.6 0 60 105 82 546.6 0 82 106 94 546.6 0 94 107 102 546.6 0 102 108 66 546.6 0 66 109 46 546.6 0 46 110 44 546.6 0 44 111 35 546.6 0 35 112 43 546.6 0 43 113 68 546.6 0 68 114 53 546.6 0 53 115 59 546.6 0 59 116 98 546.6 0 98 117 81 546.6 0 81 118 69 546.6 0 69 119 92 546.6 0 92 120 112 546.6 0 112 121 97 546.6 0 97 122 45 546.6 0 45 123 83 546.6 0 83 124 86 546.6 0 86 125 531 546.6 0 531 126 483 546.6 0 483 127 716 546.6 0 716 128 73 546.6 0 73 129 74 546.6 0 74 130 95 546.6 0 95 131 69 546.6 0 69 132 67 546.6 0 67 133 120 546.6 0 120 134 114 546.6 0 114 135 228 546.6 0 228 136 200 546.6 0 200 137 72 546.6 0 72 138 91 546.6 0 91 139 71 546.6 0 71 140 75 546.6 0 75 141 84 546.6 0 84 142 92 546.6 0 92 143 95 546.6 0 95 144 126 546.6 0 126 145 826 546.6 0 826 146 418 546.6 0 418 147 935 546.6 0 935 148 95 546.6 0 95 149 63 546.6 0 63 150 71 546.6 0 71 151 85 546.6 0 85 152 71 546.6 0 71 153 71 546.6 0 71 154 108 546.6 0 108 155 132 546.6 0 132 156 113 546.6 0 113 157 203 546.6 0 203 158 117 546.6 0 117 159 137 546.6 0 137 160 83 546.6 0 83 161 113 546.6 0 113 162 101 546.6 0 101 163 103 546.6 0 103 164 110 546.6 0 110 165 232 546.6 0 232 166 160 546.6 0 160 167 162 546.6 0 162 168 86 546.6 0 86 169 75 546.6 0 75 170 78 546.6 0 78 171 95 546.6 0 95 172 184 546.6 0 184 173 652 546.6 0 652 174 1425 546.6 0 1425 175 696 546.6 0 696 176 501 546.6 0 501 177 78 546.6 0 78 178 105 546.6 0 105 179 92 546.6 0 92 180 135 546.6 0 135 181 386 546.6 0 386 182 1065 546.6 0 1065 183 981 546.6 0 981 184 259 546.6 0 259 185 152 546.6 0 152 186 606 546.6 0 606 187 678 546.6 0 678 188 1049 546.6 0 1049 189 279 546.6 0 279 190 242 546.6 0 242 191 243 546.6 0 243 192 139 546.6 0 139 193 135 546.6 0 135 194 122 546.6 0 122 195 125 546.6 0 125 196 105 546.6 0 105 197 72 546.6 0 72 198 57 546.6 0 57 199 99 546.6 0 99 200 118 546.6 0 118 201 157 546.6 0 157 202 127 546.6 0 127 203 144 546.6 0 144 204 132 546.6 0 132 205 126 546.6 0 126 206 553 546.6 0 553 207 449 546.6 0 449 208 913 546.6 0 913 209 418 546.6 0 418 210 74 546.6 0 74 211 88 546.6 0 88 212 137 546.6 0 137 213 178 546.6 0 178 214 154 546.6 0 154 215 223 546.6 0 223 216 151 546.6 0 151 217 130 546.6 0 130 218 148 546.6 0 148 219 199 546.6 0 199 220 250 546.6 0 250 221 288 546.6 0 288 222 242 546.6 0 242 223 179 546.6 0 179 224 292 546.6 0 292 225 2361 546.6 0 2361 226 4271 546.6 0 4271 227 5101 546.6 0 5101 228 240 546.6 0 240 229 348 546.6 0 348 230 1689 546.6 0 1689 231 5314 546.6 0 5314 232 2479 546.6 0 2479 233 617 546.6 0 617 234 625 546.6 0 625 235 414 546.6 0 414 236 134 546.6 0 134 237 117 546.6 0 117 238 104 546.6 0 104 239 118 546.6 0 118 240 194 546.6 0 194 241 168 546.6 0 168 242 147 546.6 0 147 243 172 546.6 0 172 244 168 546.6 0 168 245 212 546.6 0 212 246 284 546.6 0 284 247 305 546.6 0 305 248 336 546.6 0 336 249 212 546.6 0 212 250 813 546.6 0 813 251 842 546.6 0 842 252 1526 546.6 0 1526 253 1264 546.6 0 1264 254 1164 546.6 0 1164 255 436 546.6 0 436 256 101 546.6 0 101 257 93 546.6 0 93 258 109 546.6 0 109 259 115 546.6 0 115 260 136 546.6 0 136 261 132 546.6 0 132 262 177 546.6 0 177 263 166 546.6 0 166 264 332 546.6 0 332 265 274 546.6 0 274 266 137 546.6 0 137 267 186 546.6 0 186 268 264 546.6 0 264 269 211 546.6 0 211 270 161 546.6 0 161 271 208 546.6 0 208 272 233 546.6 0 233 273 240 546.6 0 240 274 198 546.6 0 198 275 422 546.6 0 422 276 332 546.6 0 332 277 271 546.6 0 271 278 429 546.6 0 429 279 1793 546.6 0 1793 280 5169 546.6 0 5169 281 3899 546.6 0 3899 282 2343 546.6 0 2343 283 591 546.6 0 591 284 302 546.6 0 302 285 532 546.6 0 532 286 277 546.6 0 277 287 348 546.6 0 348 288 217 546.6 0 217 289 258 546.6 0 258 290 354 546.6 0 354 291 221 546.6 0 221 292 213 546.6 0 213 293 157 546.6 0 157 294 272 546.6 0 272 295 422 546.6 0 422 296 532 546.6 0 532 297 744 546.6 0 744 298 1087 546.6 0 1087 299 1518 546.6 0 1518 300 3305 546.6 0 3305 301 21117 546.6 0 21117 Finished in 0.93 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 26,042 Reads with adapters: 37 (0.1%) Reads that were too short: 19 (0.1%) Reads written (passing filters): 18 (0.1%) Total basepairs processed: 4,159,031 bp Total written (filtered): 4,316 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 13 0.0 4 9 2 1 0 1 28 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 42 2 0.0 4 0 1 0 0 1 49 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 1 188 1 0.0 4 0 0 1 208 1 0.0 4 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (556 us/read; 0.11 M reads/minute). === Summary === Total reads processed: 18 Reads with adapters: 2 (11.1%) Reads written (passing filters): 18 (100.0%) Total basepairs processed: 4,316 bp Total written (filtered): 4,276 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 50.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 0 1 29 1 0.0 1 0 1