This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.46 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 126,282 (5.6%) Reads that were too short: 105,519 (4.7%) Reads written (passing filters): 20,763 (0.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 3,538,046 bp (0.5%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 126282 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 61 546.6 0 61 7 39 546.6 0 39 8 70 546.6 0 70 9 49 546.6 0 49 10 96 546.6 0 96 11 65 546.6 0 65 12 35 546.6 0 35 13 75 546.6 0 75 14 96 546.6 0 96 15 55 546.6 0 55 16 53 546.6 0 53 17 73 546.6 0 73 18 245 546.6 0 245 19 35 546.6 0 35 20 52 546.6 0 52 21 64 546.6 0 64 22 42 546.6 0 42 23 36 546.6 0 36 24 43 546.6 0 43 25 31 546.6 0 31 26 42 546.6 0 42 27 23 546.6 0 23 28 34 546.6 0 34 29 27 546.6 0 27 30 49 546.6 0 49 31 65 546.6 0 65 32 28 546.6 0 28 33 35 546.6 0 35 34 40 546.6 0 40 35 32 546.6 0 32 36 19 546.6 0 19 37 51 546.6 0 51 38 84 546.6 0 84 39 39 546.6 0 39 40 24 546.6 0 24 41 47 546.6 0 47 42 22 546.6 0 22 43 116 546.6 0 116 44 55 546.6 0 55 45 60 546.6 0 60 46 531 546.6 0 531 47 130 546.6 0 130 48 98 546.6 0 98 49 41 546.6 0 41 50 57 546.6 0 57 51 51 546.6 0 51 52 40 546.6 0 40 53 29 546.6 0 29 54 76 546.6 0 76 55 72 546.6 0 72 56 47 546.6 0 47 57 57 546.6 0 57 58 66 546.6 0 66 59 33 546.6 0 33 60 55 546.6 0 55 61 39 546.6 0 39 62 59 546.6 0 59 63 25 546.6 0 25 64 37 546.6 0 37 65 35 546.6 0 35 66 42 546.6 0 42 67 45 546.6 0 45 68 43 546.6 0 43 69 48 546.6 0 48 70 51 546.6 0 51 71 63 546.6 0 63 72 54 546.6 0 54 73 77 546.6 0 77 74 49 546.6 0 49 75 77 546.6 0 77 76 54 546.6 0 54 77 52 546.6 0 52 78 64 546.6 0 64 79 40 546.6 0 40 80 45 546.6 0 45 81 36 546.6 0 36 82 47 546.6 0 47 83 41 546.6 0 41 84 54 546.6 0 54 85 47 546.6 0 47 86 44 546.6 0 44 87 50 546.6 0 50 88 66 546.6 0 66 89 58 546.6 0 58 90 50 546.6 0 50 91 42 546.6 0 42 92 44 546.6 0 44 93 60 546.6 0 60 94 56 546.6 0 56 95 72 546.6 0 72 96 53 546.6 0 53 97 130 546.6 0 130 98 56 546.6 0 56 99 103 546.6 0 103 100 43 546.6 0 43 101 42 546.6 0 42 102 41 546.6 0 41 103 55 546.6 0 55 104 64 546.6 0 64 105 36 546.6 0 36 106 106 546.6 0 106 107 70 546.6 0 70 108 56 546.6 0 56 109 45 546.6 0 45 110 37 546.6 0 37 111 52 546.6 0 52 112 45 546.6 0 45 113 47 546.6 0 47 114 40 546.6 0 40 115 62 546.6 0 62 116 73 546.6 0 73 117 70 546.6 0 70 118 46 546.6 0 46 119 107 546.6 0 107 120 130 546.6 0 130 121 70 546.6 0 70 122 55 546.6 0 55 123 57 546.6 0 57 124 60 546.6 0 60 125 498 546.6 0 498 126 423 546.6 0 423 127 655 546.6 0 655 128 51 546.6 0 51 129 53 546.6 0 53 130 79 546.6 0 79 131 53 546.6 0 53 132 47 546.6 0 47 133 42 546.6 0 42 134 91 546.6 0 91 135 116 546.6 0 116 136 124 546.6 0 124 137 62 546.6 0 62 138 74 546.6 0 74 139 71 546.6 0 71 140 46 546.6 0 46 141 58 546.6 0 58 142 74 546.6 0 74 143 61 546.6 0 61 144 58 546.6 0 58 145 365 546.6 0 365 146 233 546.6 0 233 147 528 546.6 0 528 148 65 546.6 0 65 149 57 546.6 0 57 150 62 546.6 0 62 151 50 546.6 0 50 152 74 546.6 0 74 153 59 546.6 0 59 154 90 546.6 0 90 155 61 546.6 0 61 156 75 546.6 0 75 157 103 546.6 0 103 158 68 546.6 0 68 159 91 546.6 0 91 160 66 546.6 0 66 161 63 546.6 0 63 162 75 546.6 0 75 163 90 546.6 0 90 164 106 546.6 0 106 165 119 546.6 0 119 166 88 546.6 0 88 167 81 546.6 0 81 168 70 546.6 0 70 169 70 546.6 0 70 170 61 546.6 0 61 171 77 546.6 0 77 172 142 546.6 0 142 173 558 546.6 0 558 174 1104 546.6 0 1104 175 491 546.6 0 491 176 277 546.6 0 277 177 64 546.6 0 64 178 105 546.6 0 105 179 115 546.6 0 115 180 137 546.6 0 137 181 297 546.6 0 297 182 846 546.6 0 846 183 807 546.6 0 807 184 177 546.6 0 177 185 84 546.6 0 84 186 251 546.6 0 251 187 550 546.6 0 550 188 661 546.6 0 661 189 203 546.6 0 203 190 114 546.6 0 114 191 120 546.6 0 120 192 113 546.6 0 113 193 95 546.6 0 95 194 93 546.6 0 93 195 80 546.6 0 80 196 59 546.6 0 59 197 57 546.6 0 57 198 44 546.6 0 44 199 71 546.6 0 71 200 69 546.6 0 69 201 89 546.6 0 89 202 93 546.6 0 93 203 104 546.6 0 104 204 89 546.6 0 89 205 74 546.6 0 74 206 157 546.6 0 157 207 143 546.6 0 143 208 326 546.6 0 326 209 198 546.6 0 198 210 53 546.6 0 53 211 90 546.6 0 90 212 114 546.6 0 114 213 123 546.6 0 123 214 139 546.6 0 139 215 174 546.6 0 174 216 146 546.6 0 146 217 95 546.6 0 95 218 114 546.6 0 114 219 140 546.6 0 140 220 186 546.6 0 186 221 176 546.6 0 176 222 170 546.6 0 170 223 152 546.6 0 152 224 255 546.6 0 255 225 2523 546.6 0 2523 226 5115 546.6 0 5115 227 6179 546.6 0 6179 228 148 546.6 0 148 229 307 546.6 0 307 230 1552 546.6 0 1552 231 6564 546.6 0 6564 232 875 546.6 0 875 233 274 546.6 0 274 234 226 546.6 0 226 235 172 546.6 0 172 236 87 546.6 0 87 237 78 546.6 0 78 238 78 546.6 0 78 239 69 546.6 0 69 240 82 546.6 0 82 241 90 546.6 0 90 242 106 546.6 0 106 243 108 546.6 0 108 244 125 546.6 0 125 245 132 546.6 0 132 246 176 546.6 0 176 247 186 546.6 0 186 248 244 546.6 0 244 249 156 546.6 0 156 250 430 546.6 0 430 251 929 546.6 0 929 252 1148 546.6 0 1148 253 682 546.6 0 682 254 538 546.6 0 538 255 227 546.6 0 227 256 78 546.6 0 78 257 82 546.6 0 82 258 87 546.6 0 87 259 104 546.6 0 104 260 104 546.6 0 104 261 68 546.6 0 68 262 88 546.6 0 88 263 109 546.6 0 109 264 182 546.6 0 182 265 433 546.6 0 433 266 157 546.6 0 157 267 160 546.6 0 160 268 194 546.6 0 194 269 179 546.6 0 179 270 118 546.6 0 118 271 172 546.6 0 172 272 242 546.6 0 242 273 200 546.6 0 200 274 234 546.6 0 234 275 703 546.6 0 703 276 306 546.6 0 306 277 144 546.6 0 144 278 191 546.6 0 191 279 596 546.6 0 596 280 1765 546.6 0 1765 281 1497 546.6 0 1497 282 785 546.6 0 785 283 355 546.6 0 355 284 446 546.6 0 446 285 1156 546.6 0 1156 286 495 546.6 0 495 287 545 546.6 0 545 288 421 546.6 0 421 289 424 546.6 0 424 290 626 546.6 0 626 291 370 546.6 0 370 292 284 546.6 0 284 293 214 546.6 0 214 294 330 546.6 0 330 295 636 546.6 0 636 296 746 546.6 0 746 297 1343 546.6 0 1343 298 2150 546.6 0 2150 299 3142 546.6 0 3142 300 7007 546.6 0 7007 301 42934 546.6 0 42934 Finished in 0.68 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 20,763 Reads with adapters: 96 (0.5%) Reads that were too short: 33 (0.2%) Reads written (passing filters): 63 (0.3%) Total basepairs processed: 3,538,046 bp Total written (filtered): 9,699 bp (0.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 96 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 15 0.0 3 15 20 2 0.0 3 2 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 46 16 0.0 3 0 0 0 16 48 1 0.0 3 0 0 0 1 49 4 0.0 3 0 0 0 4 66 15 0.0 3 0 0 0 15 67 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 97 8 0.0 3 0 0 0 8 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 5 0.0 3 0 0 0 5 148 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 180 1 0.0 3 1 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 186 1 0.0 3 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (159 us/read; 0.38 M reads/minute). === Summary === Total reads processed: 63 Reads with adapters: 1 (1.6%) Reads written (passing filters): 63 (100.0%) Total basepairs processed: 9,699 bp Total written (filtered): 9,696 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1.0 0 1