This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.82 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 70,739 (3.2%) Reads that were too short: 24,884 (1.1%) Reads written (passing filters): 45,855 (2.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 12,101,164 bp (1.8%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 70739 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 564 546.6 0 564 7 16854 546.6 0 16854 8 15717 546.6 0 15717 9 50 546.6 0 50 10 44 546.6 0 44 11 31 546.6 0 31 12 34 546.6 0 34 13 38 546.6 0 38 14 26 546.6 0 26 15 32 546.6 0 32 16 15 546.6 0 15 17 19 546.6 0 19 18 32 546.6 0 32 19 41 546.6 0 41 20 37 546.6 0 37 21 37 546.6 0 37 22 41 546.6 0 41 23 40 546.6 0 40 24 32 546.6 0 32 25 30 546.6 0 30 26 41 546.6 0 41 27 31 546.6 0 31 28 33 546.6 0 33 29 18 546.6 0 18 30 17 546.6 0 17 31 31 546.6 0 31 32 41 546.6 0 41 33 22 546.6 0 22 34 29 546.6 0 29 35 25 546.6 0 25 36 30 546.6 0 30 37 27 546.6 0 27 38 57 546.6 0 57 39 117 546.6 0 117 40 293 546.6 0 293 41 947 546.6 0 947 42 39 546.6 0 39 43 98 546.6 0 98 44 31 546.6 0 31 45 23 546.6 0 23 46 31 546.6 0 31 47 54 546.6 0 54 48 27 546.6 0 27 49 23 546.6 0 23 50 31 546.6 0 31 51 49 546.6 0 49 52 82 546.6 0 82 53 39 546.6 0 39 54 48 546.6 0 48 55 21 546.6 0 21 56 27 546.6 0 27 57 37 546.6 0 37 58 44 546.6 0 44 59 30 546.6 0 30 60 45 546.6 0 45 61 37 546.6 0 37 62 24 546.6 0 24 63 28 546.6 0 28 64 24 546.6 0 24 65 43 546.6 0 43 66 33 546.6 0 33 67 27 546.6 0 27 68 33 546.6 0 33 69 33 546.6 0 33 70 51 546.6 0 51 71 51 546.6 0 51 72 61 546.6 0 61 73 29 546.6 0 29 74 68 546.6 0 68 75 30 546.6 0 30 76 27 546.6 0 27 77 33 546.6 0 33 78 25 546.6 0 25 79 29 546.6 0 29 80 38 546.6 0 38 81 29 546.6 0 29 82 40 546.6 0 40 83 46 546.6 0 46 84 71 546.6 0 71 85 57 546.6 0 57 86 50 546.6 0 50 87 30 546.6 0 30 88 23 546.6 0 23 89 36 546.6 0 36 90 49 546.6 0 49 91 50 546.6 0 50 92 57 546.6 0 57 93 94 546.6 0 94 94 47 546.6 0 47 95 36 546.6 0 36 96 53 546.6 0 53 97 48 546.6 0 48 98 175 546.6 0 175 99 206 546.6 0 206 100 351 546.6 0 351 101 75 546.6 0 75 102 89 546.6 0 89 103 31 546.6 0 31 104 38 546.6 0 38 105 28 546.6 0 28 106 29 546.6 0 29 107 41 546.6 0 41 108 38 546.6 0 38 109 37 546.6 0 37 110 55 546.6 0 55 111 76 546.6 0 76 112 132 546.6 0 132 113 212 546.6 0 212 114 41 546.6 0 41 115 49 546.6 0 49 116 38 546.6 0 38 117 41 546.6 0 41 118 52 546.6 0 52 119 34 546.6 0 34 120 54 546.6 0 54 121 40 546.6 0 40 122 34 546.6 0 34 123 47 546.6 0 47 124 56 546.6 0 56 125 56 546.6 0 56 126 33 546.6 0 33 127 28 546.6 0 28 128 21 546.6 0 21 129 33 546.6 0 33 130 50 546.6 0 50 131 32 546.6 0 32 132 74 546.6 0 74 133 81 546.6 0 81 134 75 546.6 0 75 135 193 546.6 0 193 136 48 546.6 0 48 137 72 546.6 0 72 138 65 546.6 0 65 139 76 546.6 0 76 140 30 546.6 0 30 141 39 546.6 0 39 142 52 546.6 0 52 143 86 546.6 0 86 144 80 546.6 0 80 145 58 546.6 0 58 146 51 546.6 0 51 147 54 546.6 0 54 148 305 546.6 0 305 149 50 546.6 0 50 150 50 546.6 0 50 151 45 546.6 0 45 152 38 546.6 0 38 153 40 546.6 0 40 154 54 546.6 0 54 155 48 546.6 0 48 156 62 546.6 0 62 157 60 546.6 0 60 158 227 546.6 0 227 159 84 546.6 0 84 160 212 546.6 0 212 161 30 546.6 0 30 162 55 546.6 0 55 163 60 546.6 0 60 164 56 546.6 0 56 165 50 546.6 0 50 166 56 546.6 0 56 167 56 546.6 0 56 168 46 546.6 0 46 169 61 546.6 0 61 170 90 546.6 0 90 171 98 546.6 0 98 172 95 546.6 0 95 173 38 546.6 0 38 174 34 546.6 0 34 175 36 546.6 0 36 176 52 546.6 0 52 177 135 546.6 0 135 178 159 546.6 0 159 179 147 546.6 0 147 180 94 546.6 0 94 181 48 546.6 0 48 182 58 546.6 0 58 183 227 546.6 0 227 184 124 546.6 0 124 185 222 546.6 0 222 186 72 546.6 0 72 187 62 546.6 0 62 188 108 546.6 0 108 189 173 546.6 0 173 190 103 546.6 0 103 191 57 546.6 0 57 192 78 546.6 0 78 193 84 546.6 0 84 194 51 546.6 0 51 195 58 546.6 0 58 196 52 546.6 0 52 197 44 546.6 0 44 198 55 546.6 0 55 199 70 546.6 0 70 200 55 546.6 0 55 201 53 546.6 0 53 202 54 546.6 0 54 203 63 546.6 0 63 204 59 546.6 0 59 205 50 546.6 0 50 206 47 546.6 0 47 207 57 546.6 0 57 208 68 546.6 0 68 209 68 546.6 0 68 210 132 546.6 0 132 211 189 546.6 0 189 212 118 546.6 0 118 213 136 546.6 0 136 214 596 546.6 0 596 215 633 546.6 0 633 216 1613 546.6 0 1613 217 168 546.6 0 168 218 55 546.6 0 55 219 61 546.6 0 61 220 67 546.6 0 67 221 58 546.6 0 58 222 109 546.6 0 109 223 716 546.6 0 716 224 249 546.6 0 249 225 1078 546.6 0 1078 226 72 546.6 0 72 227 56 546.6 0 56 228 90 546.6 0 90 229 54 546.6 0 54 230 71 546.6 0 71 231 48 546.6 0 48 232 60 546.6 0 60 233 51 546.6 0 51 234 52 546.6 0 52 235 55 546.6 0 55 236 66 546.6 0 66 237 82 546.6 0 82 238 113 546.6 0 113 239 129 546.6 0 129 240 118 546.6 0 118 241 144 546.6 0 144 242 137 546.6 0 137 243 57 546.6 0 57 244 55 546.6 0 55 245 72 546.6 0 72 246 42 546.6 0 42 247 89 546.6 0 89 248 56 546.6 0 56 249 59 546.6 0 59 250 86 546.6 0 86 251 265 546.6 0 265 252 60 546.6 0 60 253 68 546.6 0 68 254 55 546.6 0 55 255 82 546.6 0 82 256 92 546.6 0 92 257 120 546.6 0 120 258 155 546.6 0 155 259 104 546.6 0 104 260 107 546.6 0 107 261 64 546.6 0 64 262 101 546.6 0 101 263 249 546.6 0 249 264 375 546.6 0 375 265 478 546.6 0 478 266 72 546.6 0 72 267 69 546.6 0 69 268 63 546.6 0 63 269 91 546.6 0 91 270 174 546.6 0 174 271 433 546.6 0 433 272 4263 546.6 0 4263 273 2198 546.6 0 2198 274 1997 546.6 0 1997 275 101 546.6 0 101 276 242 546.6 0 242 277 357 546.6 0 357 278 518 546.6 0 518 279 131 546.6 0 131 280 129 546.6 0 129 281 57 546.6 0 57 282 65 546.6 0 65 283 120 546.6 0 120 284 236 546.6 0 236 285 361 546.6 0 361 286 236 546.6 0 236 287 116 546.6 0 116 288 159 546.6 0 159 289 101 546.6 0 101 290 68 546.6 0 68 291 59 546.6 0 59 292 56 546.6 0 56 293 76 546.6 0 76 294 76 546.6 0 76 295 72 546.6 0 72 296 68 546.6 0 68 297 103 546.6 0 103 298 186 546.6 0 186 299 447 546.6 0 447 300 324 546.6 0 324 301 327 546.6 0 327 Finished in 2.34 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 45,855 Reads with adapters: 16,629 (36.3%) Reads that were too short: 25 (0.1%) Reads written (passing filters): 16,604 (36.2%) Total basepairs processed: 12,101,164 bp Total written (filtered): 4,477,974 bp (37.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16629 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 8 0.0 3 0 0 4 3 1 19 195 0.0 3 0 156 22 11 6 20 15791 0.0 4 14722 838 173 51 7 21 42 0.0 4 6 29 3 2 2 22 5 0.0 4 0 0 4 1 23 41 0.0 4 0 0 0 27 14 36 13 0.0 4 0 0 0 0 13 45 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 492 0.0 4 0 0 0 0 492 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 1 157 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 1 217 1 0.0 4 0 0 0 0 1 223 1 0.0 4 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 1 5 229 4 0.0 4 0 0 0 1 3 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.59 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 16,604 Reads with adapters: 2,783 (16.8%) Reads written (passing filters): 16,604 (100.0%) Total basepairs processed: 4,477,974 bp Total written (filtered): 4,444,420 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2783 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 9.3% G: 84.5% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 32 259.4 0 32 4 5 64.9 0 5 8 1 0.3 0 1 9 56 0.1 0 1 55 10 706 0.0 1 67 639 11 1908 0.0 1 82 1826 12 50 0.0 1 3 47 13 1 0.0 1 0 1 14 1 0.0 1 0 1 29 1 0.0 1 0 1 114 1 0.0 1 1 151 6 0.0 1 5 1 170 1 0.0 1 1 178 1 0.0 1 1 209 3 0.0 1 2 1 210 1 0.0 1 1 224 8 0.0 1 7 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.