This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.87 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 67,806 (3.0%) Reads that were too short: 20,479 (0.9%) Reads written (passing filters): 47,327 (2.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 12,589,451 bp (1.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 67806 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 465 546.6 0 465 7 16229 546.6 0 16229 8 17703 546.6 0 17703 9 61 546.6 0 61 10 49 546.6 0 49 11 39 546.6 0 39 12 42 546.6 0 42 13 45 546.6 0 45 14 40 546.6 0 40 15 42 546.6 0 42 16 36 546.6 0 36 17 32 546.6 0 32 18 43 546.6 0 43 19 39 546.6 0 39 20 31 546.6 0 31 21 65 546.6 0 65 22 50 546.6 0 50 23 41 546.6 0 41 24 42 546.6 0 42 25 34 546.6 0 34 26 44 546.6 0 44 27 39 546.6 0 39 28 46 546.6 0 46 29 46 546.6 0 46 30 45 546.6 0 45 31 43 546.6 0 43 32 45 546.6 0 45 33 41 546.6 0 41 34 31 546.6 0 31 35 45 546.6 0 45 36 40 546.6 0 40 37 35 546.6 0 35 38 55 546.6 0 55 39 161 546.6 0 161 40 216 546.6 0 216 41 623 546.6 0 623 42 47 546.6 0 47 43 102 546.6 0 102 44 38 546.6 0 38 45 46 546.6 0 46 46 63 546.6 0 63 47 48 546.6 0 48 48 28 546.6 0 28 49 27 546.6 0 27 50 25 546.6 0 25 51 38 546.6 0 38 52 52 546.6 0 52 53 57 546.6 0 57 54 56 546.6 0 56 55 41 546.6 0 41 56 37 546.6 0 37 57 54 546.6 0 54 58 41 546.6 0 41 59 43 546.6 0 43 60 50 546.6 0 50 61 39 546.6 0 39 62 28 546.6 0 28 63 38 546.6 0 38 64 57 546.6 0 57 65 570 546.6 0 570 66 124 546.6 0 124 67 42 546.6 0 42 68 46 546.6 0 46 69 44 546.6 0 44 70 77 546.6 0 77 71 67 546.6 0 67 72 72 546.6 0 72 73 45 546.6 0 45 74 63 546.6 0 63 75 43 546.6 0 43 76 53 546.6 0 53 77 40 546.6 0 40 78 41 546.6 0 41 79 50 546.6 0 50 80 73 546.6 0 73 81 46 546.6 0 46 82 64 546.6 0 64 83 49 546.6 0 49 84 39 546.6 0 39 85 49 546.6 0 49 86 37 546.6 0 37 87 46 546.6 0 46 88 43 546.6 0 43 89 33 546.6 0 33 90 50 546.6 0 50 91 62 546.6 0 62 92 66 546.6 0 66 93 76 546.6 0 76 94 47 546.6 0 47 95 54 546.6 0 54 96 56 546.6 0 56 97 62 546.6 0 62 98 108 546.6 0 108 99 151 546.6 0 151 100 187 546.6 0 187 101 59 546.6 0 59 102 64 546.6 0 64 103 51 546.6 0 51 104 51 546.6 0 51 105 56 546.6 0 56 106 37 546.6 0 37 107 58 546.6 0 58 108 46 546.6 0 46 109 38 546.6 0 38 110 59 546.6 0 59 111 52 546.6 0 52 112 74 546.6 0 74 113 118 546.6 0 118 114 49 546.6 0 49 115 46 546.6 0 46 116 45 546.6 0 45 117 55 546.6 0 55 118 47 546.6 0 47 119 49 546.6 0 49 120 40 546.6 0 40 121 50 546.6 0 50 122 38 546.6 0 38 123 47 546.6 0 47 124 71 546.6 0 71 125 58 546.6 0 58 126 40 546.6 0 40 127 43 546.6 0 43 128 42 546.6 0 42 129 45 546.6 0 45 130 43 546.6 0 43 131 58 546.6 0 58 132 48 546.6 0 48 133 96 546.6 0 96 134 56 546.6 0 56 135 245 546.6 0 245 136 42 546.6 0 42 137 41 546.6 0 41 138 64 546.6 0 64 139 62 546.6 0 62 140 33 546.6 0 33 141 34 546.6 0 34 142 59 546.6 0 59 143 72 546.6 0 72 144 48 546.6 0 48 145 56 546.6 0 56 146 41 546.6 0 41 147 59 546.6 0 59 148 342 546.6 0 342 149 70 546.6 0 70 150 54 546.6 0 54 151 49 546.6 0 49 152 45 546.6 0 45 153 50 546.6 0 50 154 55 546.6 0 55 155 45 546.6 0 45 156 63 546.6 0 63 157 63 546.6 0 63 158 209 546.6 0 209 159 75 546.6 0 75 160 175 546.6 0 175 161 35 546.6 0 35 162 55 546.6 0 55 163 46 546.6 0 46 164 46 546.6 0 46 165 43 546.6 0 43 166 49 546.6 0 49 167 50 546.6 0 50 168 48 546.6 0 48 169 59 546.6 0 59 170 81 546.6 0 81 171 72 546.6 0 72 172 75 546.6 0 75 173 54 546.6 0 54 174 43 546.6 0 43 175 37 546.6 0 37 176 52 546.6 0 52 177 72 546.6 0 72 178 106 546.6 0 106 179 102 546.6 0 102 180 59 546.6 0 59 181 67 546.6 0 67 182 52 546.6 0 52 183 180 546.6 0 180 184 137 546.6 0 137 185 214 546.6 0 214 186 75 546.6 0 75 187 88 546.6 0 88 188 71 546.6 0 71 189 97 546.6 0 97 190 60 546.6 0 60 191 54 546.6 0 54 192 87 546.6 0 87 193 87 546.6 0 87 194 48 546.6 0 48 195 57 546.6 0 57 196 47 546.6 0 47 197 51 546.6 0 51 198 40 546.6 0 40 199 44 546.6 0 44 200 45 546.6 0 45 201 49 546.6 0 49 202 39 546.6 0 39 203 53 546.6 0 53 204 53 546.6 0 53 205 46 546.6 0 46 206 35 546.6 0 35 207 37 546.6 0 37 208 44 546.6 0 44 209 45 546.6 0 45 210 104 546.6 0 104 211 111 546.6 0 111 212 75 546.6 0 75 213 63 546.6 0 63 214 239 546.6 0 239 215 338 546.6 0 338 216 552 546.6 0 552 217 77 546.6 0 77 218 50 546.6 0 50 219 54 546.6 0 54 220 63 546.6 0 63 221 47 546.6 0 47 222 105 546.6 0 105 223 651 546.6 0 651 224 266 546.6 0 266 225 927 546.6 0 927 226 40 546.6 0 40 227 45 546.6 0 45 228 63 546.6 0 63 229 42 546.6 0 42 230 65 546.6 0 65 231 31 546.6 0 31 232 54 546.6 0 54 233 40 546.6 0 40 234 56 546.6 0 56 235 47 546.6 0 47 236 48 546.6 0 48 237 53 546.6 0 53 238 90 546.6 0 90 239 132 546.6 0 132 240 81 546.6 0 81 241 75 546.6 0 75 242 86 546.6 0 86 243 52 546.6 0 52 244 36 546.6 0 36 245 70 546.6 0 70 246 45 546.6 0 45 247 51 546.6 0 51 248 48 546.6 0 48 249 36 546.6 0 36 250 75 546.6 0 75 251 168 546.6 0 168 252 48 546.6 0 48 253 52 546.6 0 52 254 39 546.6 0 39 255 68 546.6 0 68 256 60 546.6 0 60 257 97 546.6 0 97 258 72 546.6 0 72 259 55 546.6 0 55 260 79 546.6 0 79 261 71 546.6 0 71 262 112 546.6 0 112 263 265 546.6 0 265 264 536 546.6 0 536 265 468 546.6 0 468 266 48 546.6 0 48 267 67 546.6 0 67 268 63 546.6 0 63 269 91 546.6 0 91 270 162 546.6 0 162 271 450 546.6 0 450 272 4527 546.6 0 4527 273 2472 546.6 0 2472 274 2091 546.6 0 2091 275 94 546.6 0 94 276 73 546.6 0 73 277 144 546.6 0 144 278 136 546.6 0 136 279 69 546.6 0 69 280 107 546.6 0 107 281 48 546.6 0 48 282 46 546.6 0 46 283 78 546.6 0 78 284 155 546.6 0 155 285 223 546.6 0 223 286 162 546.6 0 162 287 96 546.6 0 96 288 104 546.6 0 104 289 79 546.6 0 79 290 34 546.6 0 34 291 43 546.6 0 43 292 49 546.6 0 49 293 52 546.6 0 52 294 49 546.6 0 49 295 58 546.6 0 58 296 56 546.6 0 56 297 69 546.6 0 69 298 115 546.6 0 115 299 300 546.6 0 300 300 222 546.6 0 222 301 122 546.6 0 122 Finished in 2.19 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 47,327 Reads with adapters: 16,203 (34.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 16,185 (34.2%) Total basepairs processed: 12,589,451 bp Total written (filtered): 4,476,993 bp (35.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16203 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 97 0.0 3 0 93 4 17 15968 0.0 3 15649 307 11 1 18 46 0.0 3 2 41 3 19 9 0.0 3 0 0 9 20 19 0.0 3 0 0 0 19 30 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 67 1 0.0 3 1 68 1 0.0 3 1 73 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 4 0.0 3 0 0 0 4 85 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 6 0.0 3 0 0 0 6 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 5 0.0 3 0 0 0 5 136 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 16,185 Reads with adapters: 30 (0.2%) Reads written (passing filters): 16,185 (100.0%) Total basepairs processed: 4,476,993 bp Total written (filtered): 4,476,894 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 30 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 56.7% G: 20.0% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 252.9 0 26 5 3 15.8 0 3 6 1 4.0 0 1