This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.24 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 149,730 (6.7%) Reads that were too short: 20,389 (0.9%) Reads written (passing filters): 129,341 (5.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 22,331,275 bp (3.3%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 149730 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 149 546.6 0 149 7 100 546.6 0 100 8 99 546.6 0 99 9 130 546.6 0 130 10 61 546.6 0 61 11 100 546.6 0 100 12 88 546.6 0 88 13 69 546.6 0 69 14 63 546.6 0 63 15 86 546.6 0 86 16 68 546.6 0 68 17 79 546.6 0 79 18 83 546.6 0 83 19 80 546.6 0 80 20 94 546.6 0 94 21 202 546.6 0 202 22 327 546.6 0 327 23 282 546.6 0 282 24 517 546.6 0 517 25 1135 546.6 0 1135 26 2195 546.6 0 2195 27 89 546.6 0 89 28 68 546.6 0 68 29 72 546.6 0 72 30 59 546.6 0 59 31 71 546.6 0 71 32 77 546.6 0 77 33 79 546.6 0 79 34 52 546.6 0 52 35 78 546.6 0 78 36 63 546.6 0 63 37 76 546.6 0 76 38 57 546.6 0 57 39 66 546.6 0 66 40 77 546.6 0 77 41 88 546.6 0 88 42 70 546.6 0 70 43 78 546.6 0 78 44 58 546.6 0 58 45 73 546.6 0 73 46 69 546.6 0 69 47 80 546.6 0 80 48 57 546.6 0 57 49 101 546.6 0 101 50 73 546.6 0 73 51 96 546.6 0 96 52 72 546.6 0 72 53 78 546.6 0 78 54 105 546.6 0 105 55 125 546.6 0 125 56 137 546.6 0 137 57 279 546.6 0 279 58 95 546.6 0 95 59 89 546.6 0 89 60 95 546.6 0 95 61 289 546.6 0 289 62 653 546.6 0 653 63 618 546.6 0 618 64 81 546.6 0 81 65 76 546.6 0 76 66 129 546.6 0 129 67 102 546.6 0 102 68 201 546.6 0 201 69 201 546.6 0 201 70 269 546.6 0 269 71 275 546.6 0 275 72 108 546.6 0 108 73 144 546.6 0 144 74 124 546.6 0 124 75 110 546.6 0 110 76 118 546.6 0 118 77 87 546.6 0 87 78 229 546.6 0 229 79 114 546.6 0 114 80 195 546.6 0 195 81 159 546.6 0 159 82 293 546.6 0 293 83 946 546.6 0 946 84 2543 546.6 0 2543 85 2456 546.6 0 2456 86 117 546.6 0 117 87 96 546.6 0 96 88 123 546.6 0 123 89 107 546.6 0 107 90 89 546.6 0 89 91 63 546.6 0 63 92 88 546.6 0 88 93 83 546.6 0 83 94 74 546.6 0 74 95 79 546.6 0 79 96 90 546.6 0 90 97 91 546.6 0 91 98 121 546.6 0 121 99 220 546.6 0 220 100 123 546.6 0 123 101 142 546.6 0 142 102 190 546.6 0 190 103 128 546.6 0 128 104 102 546.6 0 102 105 128 546.6 0 128 106 140 546.6 0 140 107 197 546.6 0 197 108 176 546.6 0 176 109 198 546.6 0 198 110 312 546.6 0 312 111 316 546.6 0 316 112 3450 546.6 0 3450 113 4818 546.6 0 4818 114 16324 546.6 0 16324 115 179 546.6 0 179 116 122 546.6 0 122 117 178 546.6 0 178 118 280 546.6 0 280 119 257 546.6 0 257 120 1567 546.6 0 1567 121 1838 546.6 0 1838 122 4377 546.6 0 4377 123 409 546.6 0 409 124 499 546.6 0 499 125 2426 546.6 0 2426 126 17331 546.6 0 17331 127 6776 546.6 0 6776 128 106 546.6 0 106 129 115 546.6 0 115 130 112 546.6 0 112 131 122 546.6 0 122 132 91 546.6 0 91 133 95 546.6 0 95 134 131 546.6 0 131 135 81 546.6 0 81 136 108 546.6 0 108 137 138 546.6 0 138 138 192 546.6 0 192 139 121 546.6 0 121 140 132 546.6 0 132 141 118 546.6 0 118 142 228 546.6 0 228 143 375 546.6 0 375 144 411 546.6 0 411 145 205 546.6 0 205 146 288 546.6 0 288 147 1124 546.6 0 1124 148 1622 546.6 0 1622 149 2594 546.6 0 2594 150 1114 546.6 0 1114 151 1089 546.6 0 1089 152 1115 546.6 0 1115 153 196 546.6 0 196 154 183 546.6 0 183 155 141 546.6 0 141 156 97 546.6 0 97 157 197 546.6 0 197 158 190 546.6 0 190 159 134 546.6 0 134 160 174 546.6 0 174 161 188 546.6 0 188 162 173 546.6 0 173 163 467 546.6 0 467 164 283 546.6 0 283 165 894 546.6 0 894 166 581 546.6 0 581 167 873 546.6 0 873 168 1040 546.6 0 1040 169 887 546.6 0 887 170 1102 546.6 0 1102 171 1910 546.6 0 1910 172 1214 546.6 0 1214 173 2282 546.6 0 2282 174 421 546.6 0 421 175 451 546.6 0 451 176 341 546.6 0 341 177 158 546.6 0 158 178 162 546.6 0 162 179 147 546.6 0 147 180 123 546.6 0 123 181 200 546.6 0 200 182 240 546.6 0 240 183 1496 546.6 0 1496 184 391 546.6 0 391 185 1206 546.6 0 1206 186 282 546.6 0 282 187 473 546.6 0 473 188 426 546.6 0 426 189 387 546.6 0 387 190 262 546.6 0 262 191 2100 546.6 0 2100 192 1608 546.6 0 1608 193 4966 546.6 0 4966 194 1647 546.6 0 1647 195 254 546.6 0 254 196 473 546.6 0 473 197 288 546.6 0 288 198 937 546.6 0 937 199 274 546.6 0 274 200 397 546.6 0 397 201 345 546.6 0 345 202 148 546.6 0 148 203 74 546.6 0 74 204 97 546.6 0 97 205 95 546.6 0 95 206 139 546.6 0 139 207 269 546.6 0 269 208 193 546.6 0 193 209 176 546.6 0 176 210 145 546.6 0 145 211 109 546.6 0 109 212 121 546.6 0 121 213 98 546.6 0 98 214 86 546.6 0 86 215 228 546.6 0 228 216 166 546.6 0 166 217 168 546.6 0 168 218 236 546.6 0 236 219 172 546.6 0 172 220 204 546.6 0 204 221 105 546.6 0 105 222 101 546.6 0 101 223 117 546.6 0 117 224 142 546.6 0 142 225 212 546.6 0 212 226 242 546.6 0 242 227 319 546.6 0 319 228 489 546.6 0 489 229 261 546.6 0 261 230 427 546.6 0 427 231 111 546.6 0 111 232 90 546.6 0 90 233 106 546.6 0 106 234 89 546.6 0 89 235 118 546.6 0 118 236 105 546.6 0 105 237 104 546.6 0 104 238 105 546.6 0 105 239 109 546.6 0 109 240 92 546.6 0 92 241 78 546.6 0 78 242 100 546.6 0 100 243 200 546.6 0 200 244 157 546.6 0 157 245 236 546.6 0 236 246 314 546.6 0 314 247 406 546.6 0 406 248 199 546.6 0 199 249 85 546.6 0 85 250 98 546.6 0 98 251 149 546.6 0 149 252 161 546.6 0 161 253 188 546.6 0 188 254 141 546.6 0 141 255 135 546.6 0 135 256 199 546.6 0 199 257 221 546.6 0 221 258 135 546.6 0 135 259 120 546.6 0 120 260 121 546.6 0 121 261 122 546.6 0 122 262 116 546.6 0 116 263 153 546.6 0 153 264 157 546.6 0 157 265 171 546.6 0 171 266 156 546.6 0 156 267 623 546.6 0 623 268 1212 546.6 0 1212 269 1632 546.6 0 1632 270 184 546.6 0 184 271 91 546.6 0 91 272 83 546.6 0 83 273 114 546.6 0 114 274 102 546.6 0 102 275 113 546.6 0 113 276 159 546.6 0 159 277 181 546.6 0 181 278 159 546.6 0 159 279 205 546.6 0 205 280 173 546.6 0 173 281 184 546.6 0 184 282 268 546.6 0 268 283 143 546.6 0 143 284 131 546.6 0 131 285 81 546.6 0 81 286 90 546.6 0 90 287 94 546.6 0 94 288 103 546.6 0 103 289 137 546.6 0 137 290 111 546.6 0 111 291 91 546.6 0 91 292 115 546.6 0 115 293 129 546.6 0 129 294 131 546.6 0 131 295 167 546.6 0 167 296 161 546.6 0 161 297 156 546.6 0 156 298 589 546.6 0 589 299 1509 546.6 0 1509 300 178 546.6 0 178 301 104 546.6 0 104 Finished in 4.79 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 129,341 Reads with adapters: 250 (0.2%) Reads that were too short: 94 (0.1%) Reads written (passing filters): 156 (0.1%) Total basepairs processed: 22,331,275 bp Total written (filtered): 27,274 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 250 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 1 0.0 3 0 1 20 13 0.0 4 9 0 4 21 1 0.0 4 0 0 0 1 22 1 0.0 4 0 0 0 0 1 23 2 0.0 4 0 0 0 0 2 24 2 0.0 4 0 0 0 0 2 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 47 2 0.0 4 0 0 0 0 2 49 4 0.0 4 0 0 0 0 4 50 6 0.0 4 0 0 0 0 6 51 91 0.0 4 0 0 0 0 91 53 1 0.0 4 0 0 0 0 1 54 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 1 1 73 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 1 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 6 0.0 4 0 0 0 0 6 108 1 0.0 4 0 0 0 0 1 110 23 0.0 4 0 0 0 0 23 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 4 0.0 4 0 0 0 0 4 118 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 122 3 0.0 4 0 0 0 0 3 124 3 0.0 4 0 0 0 0 3 128 2 0.0 4 0 0 0 0 2 129 2 0.0 4 0 0 0 0 2 130 3 0.0 4 0 0 0 1 2 133 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 150 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 177 5 0.0 4 0 0 0 0 5 195 1 0.0 4 0 0 0 0 1 196 3 0.0 4 0 0 0 0 3 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 3 0.0 4 0 0 0 0 3 217 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 246 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 156 Reads with adapters: 4 (2.6%) Reads written (passing filters): 156 (100.0%) Total basepairs processed: 27,274 bp Total written (filtered): 27,259 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 2.4 0 2 4 1 0.6 0 1 5 1 0.2 0 1