This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.30 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 67,747 (3.0%) Reads that were too short: 26,148 (1.2%) Reads written (passing filters): 41,599 (1.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 10,253,678 bp (1.5%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 67747 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 546.6 0 172 7 372 546.6 0 372 8 12626 546.6 0 12626 9 10278 546.6 0 10278 10 75 546.6 0 75 11 56 546.6 0 56 12 67 546.6 0 67 13 50 546.6 0 50 14 99 546.6 0 99 15 87 546.6 0 87 16 75 546.6 0 75 17 55 546.6 0 55 18 77 546.6 0 77 19 71 546.6 0 71 20 63 546.6 0 63 21 67 546.6 0 67 22 73 546.6 0 73 23 81 546.6 0 81 24 74 546.6 0 74 25 84 546.6 0 84 26 67 546.6 0 67 27 53 546.6 0 53 28 47 546.6 0 47 29 74 546.6 0 74 30 66 546.6 0 66 31 56 546.6 0 56 32 58 546.6 0 58 33 48 546.6 0 48 34 73 546.6 0 73 35 70 546.6 0 70 36 74 546.6 0 74 37 83 546.6 0 83 38 57 546.6 0 57 39 62 546.6 0 62 40 57 546.6 0 57 41 69 546.6 0 69 42 57 546.6 0 57 43 53 546.6 0 53 44 74 546.6 0 74 45 86 546.6 0 86 46 71 546.6 0 71 47 73 546.6 0 73 48 79 546.6 0 79 49 74 546.6 0 74 50 59 546.6 0 59 51 66 546.6 0 66 52 58 546.6 0 58 53 76 546.6 0 76 54 79 546.6 0 79 55 72 546.6 0 72 56 73 546.6 0 73 57 45 546.6 0 45 58 78 546.6 0 78 59 209 546.6 0 209 60 158 546.6 0 158 61 152 546.6 0 152 62 80 546.6 0 80 63 72 546.6 0 72 64 62 546.6 0 62 65 61 546.6 0 61 66 91 546.6 0 91 67 94 546.6 0 94 68 65 546.6 0 65 69 91 546.6 0 91 70 160 546.6 0 160 71 89 546.6 0 89 72 107 546.6 0 107 73 69 546.6 0 69 74 84 546.6 0 84 75 106 546.6 0 106 76 170 546.6 0 170 77 169 546.6 0 169 78 298 546.6 0 298 79 61 546.6 0 61 80 131 546.6 0 131 81 90 546.6 0 90 82 96 546.6 0 96 83 74 546.6 0 74 84 91 546.6 0 91 85 108 546.6 0 108 86 65 546.6 0 65 87 61 546.6 0 61 88 63 546.6 0 63 89 78 546.6 0 78 90 77 546.6 0 77 91 88 546.6 0 88 92 108 546.6 0 108 93 73 546.6 0 73 94 95 546.6 0 95 95 81 546.6 0 81 96 95 546.6 0 95 97 67 546.6 0 67 98 86 546.6 0 86 99 73 546.6 0 73 100 75 546.6 0 75 101 72 546.6 0 72 102 81 546.6 0 81 103 109 546.6 0 109 104 56 546.6 0 56 105 78 546.6 0 78 106 77 546.6 0 77 107 83 546.6 0 83 108 77 546.6 0 77 109 85 546.6 0 85 110 77 546.6 0 77 111 98 546.6 0 98 112 135 546.6 0 135 113 95 546.6 0 95 114 97 546.6 0 97 115 94 546.6 0 94 116 69 546.6 0 69 117 91 546.6 0 91 118 86 546.6 0 86 119 83 546.6 0 83 120 72 546.6 0 72 121 77 546.6 0 77 122 62 546.6 0 62 123 64 546.6 0 64 124 89 546.6 0 89 125 75 546.6 0 75 126 91 546.6 0 91 127 76 546.6 0 76 128 75 546.6 0 75 129 104 546.6 0 104 130 157 546.6 0 157 131 140 546.6 0 140 132 170 546.6 0 170 133 159 546.6 0 159 134 163 546.6 0 163 135 116 546.6 0 116 136 91 546.6 0 91 137 75 546.6 0 75 138 223 546.6 0 223 139 187 546.6 0 187 140 118 546.6 0 118 141 108 546.6 0 108 142 87 546.6 0 87 143 124 546.6 0 124 144 149 546.6 0 149 145 120 546.6 0 120 146 154 546.6 0 154 147 101 546.6 0 101 148 82 546.6 0 82 149 78 546.6 0 78 150 64 546.6 0 64 151 108 546.6 0 108 152 115 546.6 0 115 153 105 546.6 0 105 154 82 546.6 0 82 155 105 546.6 0 105 156 110 546.6 0 110 157 83 546.6 0 83 158 340 546.6 0 340 159 106 546.6 0 106 160 73 546.6 0 73 161 71 546.6 0 71 162 104 546.6 0 104 163 65 546.6 0 65 164 82 546.6 0 82 165 90 546.6 0 90 166 66 546.6 0 66 167 62 546.6 0 62 168 79 546.6 0 79 169 74 546.6 0 74 170 66 546.6 0 66 171 76 546.6 0 76 172 80 546.6 0 80 173 85 546.6 0 85 174 78 546.6 0 78 175 70 546.6 0 70 176 86 546.6 0 86 177 89 546.6 0 89 178 75 546.6 0 75 179 63 546.6 0 63 180 89 546.6 0 89 181 89 546.6 0 89 182 81 546.6 0 81 183 96 546.6 0 96 184 66 546.6 0 66 185 63 546.6 0 63 186 75 546.6 0 75 187 71 546.6 0 71 188 84 546.6 0 84 189 76 546.6 0 76 190 106 546.6 0 106 191 202 546.6 0 202 192 222 546.6 0 222 193 146 546.6 0 146 194 285 546.6 0 285 195 77 546.6 0 77 196 87 546.6 0 87 197 285 546.6 0 285 198 192 546.6 0 192 199 272 546.6 0 272 200 68 546.6 0 68 201 73 546.6 0 73 202 88 546.6 0 88 203 70 546.6 0 70 204 144 546.6 0 144 205 92 546.6 0 92 206 166 546.6 0 166 207 66 546.6 0 66 208 56 546.6 0 56 209 66 546.6 0 66 210 63 546.6 0 63 211 102 546.6 0 102 212 86 546.6 0 86 213 66 546.6 0 66 214 91 546.6 0 91 215 72 546.6 0 72 216 72 546.6 0 72 217 67 546.6 0 67 218 89 546.6 0 89 219 65 546.6 0 65 220 78 546.6 0 78 221 91 546.6 0 91 222 89 546.6 0 89 223 211 546.6 0 211 224 135 546.6 0 135 225 363 546.6 0 363 226 66 546.6 0 66 227 92 546.6 0 92 228 76 546.6 0 76 229 73 546.6 0 73 230 55 546.6 0 55 231 78 546.6 0 78 232 92 546.6 0 92 233 128 546.6 0 128 234 177 546.6 0 177 235 149 546.6 0 149 236 99 546.6 0 99 237 185 546.6 0 185 238 203 546.6 0 203 239 141 546.6 0 141 240 136 546.6 0 136 241 120 546.6 0 120 242 131 546.6 0 131 243 150 546.6 0 150 244 202 546.6 0 202 245 124 546.6 0 124 246 120 546.6 0 120 247 163 546.6 0 163 248 117 546.6 0 117 249 104 546.6 0 104 250 97 546.6 0 97 251 78 546.6 0 78 252 88 546.6 0 88 253 63 546.6 0 63 254 151 546.6 0 151 255 167 546.6 0 167 256 246 546.6 0 246 257 180 546.6 0 180 258 308 546.6 0 308 259 186 546.6 0 186 260 170 546.6 0 170 261 197 546.6 0 197 262 183 546.6 0 183 263 185 546.6 0 185 264 177 546.6 0 177 265 1049 546.6 0 1049 266 891 546.6 0 891 267 2077 546.6 0 2077 268 344 546.6 0 344 269 57 546.6 0 57 270 98 546.6 0 98 271 141 546.6 0 141 272 197 546.6 0 197 273 237 546.6 0 237 274 225 546.6 0 225 275 182 546.6 0 182 276 108 546.6 0 108 277 115 546.6 0 115 278 111 546.6 0 111 279 142 546.6 0 142 280 157 546.6 0 157 281 158 546.6 0 158 282 121 546.6 0 121 283 145 546.6 0 145 284 118 546.6 0 118 285 148 546.6 0 148 286 159 546.6 0 159 287 154 546.6 0 154 288 203 546.6 0 203 289 197 546.6 0 197 290 221 546.6 0 221 291 289 546.6 0 289 292 242 546.6 0 242 293 286 546.6 0 286 294 342 546.6 0 342 295 298 546.6 0 298 296 260 546.6 0 260 297 459 546.6 0 459 298 1151 546.6 0 1151 299 1826 546.6 0 1826 300 3740 546.6 0 3740 301 1555 546.6 0 1555 Finished in 2.07 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 41,599 Reads with adapters: 12,188 (29.3%) Reads that were too short: 21 (0.1%) Reads written (passing filters): 12,167 (29.2%) Total basepairs processed: 10,253,678 bp Total written (filtered): 3,166,817 bp (30.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12188 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 14 0.0 3 0 0 12 1 1 19 151 0.0 3 0 132 10 5 4 20 10562 0.0 4 9876 572 78 26 10 21 28 0.0 4 9 18 1 22 2 0.0 4 0 0 2 23 57 0.0 4 0 0 0 40 17 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 1 37 1 0.0 4 1 56 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 125 3 0.0 4 0 0 0 0 3 126 18 0.0 4 0 0 0 0 18 127 1309 0.0 4 0 0 0 0 1309 128 5 0.0 4 0 0 0 0 5 130 4 0.0 4 0 0 0 1 3 132 1 0.0 4 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 181 1 0.0 4 1 183 1 0.0 4 0 1 185 1 0.0 4 1 186 2 0.0 4 2 187 1 0.0 4 0 1 188 2 0.0 4 2 190 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 270 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 12,167 Reads with adapters: 2,770 (22.8%) Reads written (passing filters): 12,167 (100.0%) Total basepairs processed: 3,166,817 bp Total written (filtered): 3,139,003 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2770 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 6.5% G: 89.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 190.1 0 5 4 1 47.5 0 1 6 1 3.0 0 1 9 815 0.0 0 82 733 10 1632 0.0 1 136 1496 11 292 0.0 1 11 281 12 13 0.0 1 0 13 13 7 0.0 1 0 7 150 2 0.0 1 2 152 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.