This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.05 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 68,035 (3.0%) Reads that were too short: 29,365 (1.3%) Reads written (passing filters): 38,670 (1.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,763,873 bp (1.4%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 68035 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 138 546.6 0 138 7 320 546.6 0 320 8 11674 546.6 0 11674 9 11980 546.6 0 11980 10 52 546.6 0 52 11 58 546.6 0 58 12 64 546.6 0 64 13 65 546.6 0 65 14 67 546.6 0 67 15 72 546.6 0 72 16 55 546.6 0 55 17 54 546.6 0 54 18 63 546.6 0 63 19 55 546.6 0 55 20 63 546.6 0 63 21 58 546.6 0 58 22 65 546.6 0 65 23 70 546.6 0 70 24 53 546.6 0 53 25 57 546.6 0 57 26 58 546.6 0 58 27 45 546.6 0 45 28 37 546.6 0 37 29 67 546.6 0 67 30 37 546.6 0 37 31 45 546.6 0 45 32 45 546.6 0 45 33 37 546.6 0 37 34 59 546.6 0 59 35 41 546.6 0 41 36 41 546.6 0 41 37 57 546.6 0 57 38 35 546.6 0 35 39 41 546.6 0 41 40 58 546.6 0 58 41 51 546.6 0 51 42 53 546.6 0 53 43 41 546.6 0 41 44 56 546.6 0 56 45 79 546.6 0 79 46 69 546.6 0 69 47 61 546.6 0 61 48 66 546.6 0 66 49 60 546.6 0 60 50 38 546.6 0 38 51 34 546.6 0 34 52 51 546.6 0 51 53 49 546.6 0 49 54 52 546.6 0 52 55 44 546.6 0 44 56 81 546.6 0 81 57 50 546.6 0 50 58 69 546.6 0 69 59 261 546.6 0 261 60 161 546.6 0 161 61 167 546.6 0 167 62 37 546.6 0 37 63 52 546.6 0 52 64 59 546.6 0 59 65 62 546.6 0 62 66 59 546.6 0 59 67 70 546.6 0 70 68 58 546.6 0 58 69 58 546.6 0 58 70 171 546.6 0 171 71 68 546.6 0 68 72 119 546.6 0 119 73 63 546.6 0 63 74 65 546.6 0 65 75 82 546.6 0 82 76 87 546.6 0 87 77 90 546.6 0 90 78 168 546.6 0 168 79 66 546.6 0 66 80 82 546.6 0 82 81 88 546.6 0 88 82 70 546.6 0 70 83 54 546.6 0 54 84 55 546.6 0 55 85 69 546.6 0 69 86 47 546.6 0 47 87 55 546.6 0 55 88 53 546.6 0 53 89 61 546.6 0 61 90 51 546.6 0 51 91 74 546.6 0 74 92 85 546.6 0 85 93 75 546.6 0 75 94 75 546.6 0 75 95 87 546.6 0 87 96 55 546.6 0 55 97 51 546.6 0 51 98 72 546.6 0 72 99 63 546.6 0 63 100 68 546.6 0 68 101 74 546.6 0 74 102 52 546.6 0 52 103 66 546.6 0 66 104 55 546.6 0 55 105 60 546.6 0 60 106 69 546.6 0 69 107 50 546.6 0 50 108 60 546.6 0 60 109 61 546.6 0 61 110 73 546.6 0 73 111 70 546.6 0 70 112 79 546.6 0 79 113 63 546.6 0 63 114 56 546.6 0 56 115 55 546.6 0 55 116 53 546.6 0 53 117 84 546.6 0 84 118 72 546.6 0 72 119 48 546.6 0 48 120 65 546.6 0 65 121 54 546.6 0 54 122 66 546.6 0 66 123 54 546.6 0 54 124 61 546.6 0 61 125 55 546.6 0 55 126 51 546.6 0 51 127 60 546.6 0 60 128 65 546.6 0 65 129 79 546.6 0 79 130 110 546.6 0 110 131 115 546.6 0 115 132 114 546.6 0 114 133 130 546.6 0 130 134 132 546.6 0 132 135 73 546.6 0 73 136 67 546.6 0 67 137 67 546.6 0 67 138 216 546.6 0 216 139 166 546.6 0 166 140 132 546.6 0 132 141 76 546.6 0 76 142 59 546.6 0 59 143 91 546.6 0 91 144 146 546.6 0 146 145 72 546.6 0 72 146 111 546.6 0 111 147 81 546.6 0 81 148 48 546.6 0 48 149 64 546.6 0 64 150 57 546.6 0 57 151 54 546.6 0 54 152 72 546.6 0 72 153 62 546.6 0 62 154 66 546.6 0 66 155 77 546.6 0 77 156 68 546.6 0 68 157 81 546.6 0 81 158 343 546.6 0 343 159 97 546.6 0 97 160 82 546.6 0 82 161 43 546.6 0 43 162 56 546.6 0 56 163 46 546.6 0 46 164 48 546.6 0 48 165 84 546.6 0 84 166 49 546.6 0 49 167 43 546.6 0 43 168 48 546.6 0 48 169 51 546.6 0 51 170 54 546.6 0 54 171 50 546.6 0 50 172 53 546.6 0 53 173 47 546.6 0 47 174 58 546.6 0 58 175 50 546.6 0 50 176 42 546.6 0 42 177 55 546.6 0 55 178 53 546.6 0 53 179 46 546.6 0 46 180 57 546.6 0 57 181 70 546.6 0 70 182 51 546.6 0 51 183 45 546.6 0 45 184 43 546.6 0 43 185 49 546.6 0 49 186 65 546.6 0 65 187 47 546.6 0 47 188 56 546.6 0 56 189 74 546.6 0 74 190 69 546.6 0 69 191 186 546.6 0 186 192 287 546.6 0 287 193 177 546.6 0 177 194 274 546.6 0 274 195 59 546.6 0 59 196 72 546.6 0 72 197 314 546.6 0 314 198 153 546.6 0 153 199 292 546.6 0 292 200 51 546.6 0 51 201 61 546.6 0 61 202 47 546.6 0 47 203 44 546.6 0 44 204 206 546.6 0 206 205 56 546.6 0 56 206 191 546.6 0 191 207 59 546.6 0 59 208 46 546.6 0 46 209 62 546.6 0 62 210 44 546.6 0 44 211 71 546.6 0 71 212 54 546.6 0 54 213 71 546.6 0 71 214 80 546.6 0 80 215 72 546.6 0 72 216 53 546.6 0 53 217 51 546.6 0 51 218 75 546.6 0 75 219 55 546.6 0 55 220 58 546.6 0 58 221 57 546.6 0 57 222 96 546.6 0 96 223 300 546.6 0 300 224 156 546.6 0 156 225 519 546.6 0 519 226 75 546.6 0 75 227 71 546.6 0 71 228 37 546.6 0 37 229 56 546.6 0 56 230 45 546.6 0 45 231 84 546.6 0 84 232 78 546.6 0 78 233 112 546.6 0 112 234 98 546.6 0 98 235 88 546.6 0 88 236 85 546.6 0 85 237 120 546.6 0 120 238 126 546.6 0 126 239 84 546.6 0 84 240 91 546.6 0 91 241 91 546.6 0 91 242 74 546.6 0 74 243 65 546.6 0 65 244 130 546.6 0 130 245 70 546.6 0 70 246 93 546.6 0 93 247 98 546.6 0 98 248 55 546.6 0 55 249 58 546.6 0 58 250 55 546.6 0 55 251 66 546.6 0 66 252 62 546.6 0 62 253 69 546.6 0 69 254 102 546.6 0 102 255 139 546.6 0 139 256 169 546.6 0 169 257 130 546.6 0 130 258 191 546.6 0 191 259 138 546.6 0 138 260 114 546.6 0 114 261 152 546.6 0 152 262 93 546.6 0 93 263 119 546.6 0 119 264 87 546.6 0 87 265 298 546.6 0 298 266 295 546.6 0 295 267 644 546.6 0 644 268 160 546.6 0 160 269 46 546.6 0 46 270 48 546.6 0 48 271 77 546.6 0 77 272 115 546.6 0 115 273 151 546.6 0 151 274 128 546.6 0 128 275 104 546.6 0 104 276 77 546.6 0 77 277 94 546.6 0 94 278 119 546.6 0 119 279 112 546.6 0 112 280 172 546.6 0 172 281 145 546.6 0 145 282 146 546.6 0 146 283 146 546.6 0 146 284 145 546.6 0 145 285 161 546.6 0 161 286 149 546.6 0 149 287 152 546.6 0 152 288 241 546.6 0 241 289 273 546.6 0 273 290 280 546.6 0 280 291 512 546.6 0 512 292 458 546.6 0 458 293 417 546.6 0 417 294 507 546.6 0 507 295 449 546.6 0 449 296 420 546.6 0 420 297 701 546.6 0 701 298 1956 546.6 0 1956 299 3081 546.6 0 3081 300 6326 546.6 0 6326 301 3867 546.6 0 3867 Finished in 1.70 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 38,670 Reads with adapters: 11,293 (29.2%) Reads that were too short: 35 (0.1%) Reads written (passing filters): 11,258 (29.1%) Total basepairs processed: 9,763,873 bp Total written (filtered): 3,104,833 bp (31.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11293 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 50 0.0 3 0 50 17 11148 0.0 3 10929 213 5 1 18 25 0.0 3 2 21 2 19 10 0.0 3 0 0 9 1 20 9 0.0 3 0 0 0 9 39 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 118 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 1 182 1 0.0 3 0 1 185 1 0.0 3 0 0 1 189 25 0.0 3 0 0 0 25 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 11,258 Reads with adapters: 68 (0.6%) Reads written (passing filters): 11,258 (100.0%) Total basepairs processed: 3,104,833 bp Total written (filtered): 3,104,586 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 68 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.5% C: 11.8% G: 60.3% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 175.9 0 26 4 41 44.0 0 41 5 1 11.0 0 1