This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.92 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 48,455 (2.2%) Reads that were too short: 27,164 (1.2%) Reads written (passing filters): 21,291 (1.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 4,010,282 bp (0.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 48455 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 144 546.6 0 144 7 615 546.6 0 615 8 642 546.6 0 642 9 46 546.6 0 46 10 38 546.6 0 38 11 54 546.6 0 54 12 61 546.6 0 61 13 45 546.6 0 45 14 56 546.6 0 56 15 33 546.6 0 33 16 48 546.6 0 48 17 46 546.6 0 46 18 52 546.6 0 52 19 49 546.6 0 49 20 30 546.6 0 30 21 42 546.6 0 42 22 48 546.6 0 48 23 54 546.6 0 54 24 47 546.6 0 47 25 46 546.6 0 46 26 39 546.6 0 39 27 38 546.6 0 38 28 43 546.6 0 43 29 76 546.6 0 76 30 60 546.6 0 60 31 36 546.6 0 36 32 47 546.6 0 47 33 30 546.6 0 30 34 26 546.6 0 26 35 45 546.6 0 45 36 48 546.6 0 48 37 51 546.6 0 51 38 45 546.6 0 45 39 47 546.6 0 47 40 55 546.6 0 55 41 101 546.6 0 101 42 69 546.6 0 69 43 79 546.6 0 79 44 57 546.6 0 57 45 38 546.6 0 38 46 56 546.6 0 56 47 52 546.6 0 52 48 44 546.6 0 44 49 51 546.6 0 51 50 60 546.6 0 60 51 51 546.6 0 51 52 66 546.6 0 66 53 92 546.6 0 92 54 158 546.6 0 158 55 54 546.6 0 54 56 92 546.6 0 92 57 42 546.6 0 42 58 47 546.6 0 47 59 60 546.6 0 60 60 50 546.6 0 50 61 45 546.6 0 45 62 73 546.6 0 73 63 120 546.6 0 120 64 1420 546.6 0 1420 65 274 546.6 0 274 66 464 546.6 0 464 67 100 546.6 0 100 68 68 546.6 0 68 69 77 546.6 0 77 70 93 546.6 0 93 71 51 546.6 0 51 72 41 546.6 0 41 73 44 546.6 0 44 74 43 546.6 0 43 75 65 546.6 0 65 76 47 546.6 0 47 77 55 546.6 0 55 78 40 546.6 0 40 79 48 546.6 0 48 80 45 546.6 0 45 81 68 546.6 0 68 82 32 546.6 0 32 83 65 546.6 0 65 84 67 546.6 0 67 85 55 546.6 0 55 86 58 546.6 0 58 87 38 546.6 0 38 88 54 546.6 0 54 89 97 546.6 0 97 90 97 546.6 0 97 91 93 546.6 0 93 92 479 546.6 0 479 93 221 546.6 0 221 94 135 546.6 0 135 95 52 546.6 0 52 96 30 546.6 0 30 97 46 546.6 0 46 98 27 546.6 0 27 99 75 546.6 0 75 100 82 546.6 0 82 101 218 546.6 0 218 102 57 546.6 0 57 103 45 546.6 0 45 104 49 546.6 0 49 105 52 546.6 0 52 106 38 546.6 0 38 107 34 546.6 0 34 108 55 546.6 0 55 109 53 546.6 0 53 110 52 546.6 0 52 111 72 546.6 0 72 112 49 546.6 0 49 113 41 546.6 0 41 114 71 546.6 0 71 115 52 546.6 0 52 116 39 546.6 0 39 117 72 546.6 0 72 118 70 546.6 0 70 119 63 546.6 0 63 120 60 546.6 0 60 121 62 546.6 0 62 122 63 546.6 0 63 123 83 546.6 0 83 124 59 546.6 0 59 125 68 546.6 0 68 126 60 546.6 0 60 127 62 546.6 0 62 128 71 546.6 0 71 129 99 546.6 0 99 130 137 546.6 0 137 131 193 546.6 0 193 132 146 546.6 0 146 133 87 546.6 0 87 134 79 546.6 0 79 135 185 546.6 0 185 136 195 546.6 0 195 137 262 546.6 0 262 138 71 546.6 0 71 139 91 546.6 0 91 140 69 546.6 0 69 141 55 546.6 0 55 142 78 546.6 0 78 143 62 546.6 0 62 144 71 546.6 0 71 145 69 546.6 0 69 146 61 546.6 0 61 147 162 546.6 0 162 148 91 546.6 0 91 149 121 546.6 0 121 150 89 546.6 0 89 151 78 546.6 0 78 152 110 546.6 0 110 153 101 546.6 0 101 154 85 546.6 0 85 155 135 546.6 0 135 156 188 546.6 0 188 157 379 546.6 0 379 158 320 546.6 0 320 159 108 546.6 0 108 160 104 546.6 0 104 161 78 546.6 0 78 162 101 546.6 0 101 163 109 546.6 0 109 164 80 546.6 0 80 165 65 546.6 0 65 166 140 546.6 0 140 167 357 546.6 0 357 168 214 546.6 0 214 169 245 546.6 0 245 170 101 546.6 0 101 171 174 546.6 0 174 172 211 546.6 0 211 173 322 546.6 0 322 174 86 546.6 0 86 175 340 546.6 0 340 176 242 546.6 0 242 177 321 546.6 0 321 178 153 546.6 0 153 179 75 546.6 0 75 180 82 546.6 0 82 181 80 546.6 0 80 182 93 546.6 0 93 183 143 546.6 0 143 184 93 546.6 0 93 185 94 546.6 0 94 186 154 546.6 0 154 187 144 546.6 0 144 188 419 546.6 0 419 189 179 546.6 0 179 190 122 546.6 0 122 191 78 546.6 0 78 192 74 546.6 0 74 193 94 546.6 0 94 194 80 546.6 0 80 195 97 546.6 0 97 196 158 546.6 0 158 197 108 546.6 0 108 198 100 546.6 0 100 199 98 546.6 0 98 200 135 546.6 0 135 201 116 546.6 0 116 202 125 546.6 0 125 203 149 546.6 0 149 204 130 546.6 0 130 205 77 546.6 0 77 206 77 546.6 0 77 207 70 546.6 0 70 208 91 546.6 0 91 209 79 546.6 0 79 210 164 546.6 0 164 211 112 546.6 0 112 212 107 546.6 0 107 213 99 546.6 0 99 214 140 546.6 0 140 215 192 546.6 0 192 216 171 546.6 0 171 217 212 546.6 0 212 218 240 546.6 0 240 219 215 546.6 0 215 220 265 546.6 0 265 221 417 546.6 0 417 222 669 546.6 0 669 223 1090 546.6 0 1090 224 583 546.6 0 583 225 364 546.6 0 364 226 336 546.6 0 336 227 164 546.6 0 164 228 169 546.6 0 169 229 101 546.6 0 101 230 97 546.6 0 97 231 100 546.6 0 100 232 94 546.6 0 94 233 90 546.6 0 90 234 137 546.6 0 137 235 106 546.6 0 106 236 115 546.6 0 115 237 162 546.6 0 162 238 119 546.6 0 119 239 152 546.6 0 152 240 161 546.6 0 161 241 704 546.6 0 704 242 532 546.6 0 532 243 1253 546.6 0 1253 244 584 546.6 0 584 245 311 546.6 0 311 246 196 546.6 0 196 247 137 546.6 0 137 248 135 546.6 0 135 249 341 546.6 0 341 250 198 546.6 0 198 251 166 546.6 0 166 252 243 546.6 0 243 253 564 546.6 0 564 254 1125 546.6 0 1125 255 187 546.6 0 187 256 194 546.6 0 194 257 145 546.6 0 145 258 105 546.6 0 105 259 68 546.6 0 68 260 91 546.6 0 91 261 148 546.6 0 148 262 198 546.6 0 198 263 227 546.6 0 227 264 241 546.6 0 241 265 219 546.6 0 219 266 304 546.6 0 304 267 111 546.6 0 111 268 261 546.6 0 261 269 414 546.6 0 414 270 1304 546.6 0 1304 271 451 546.6 0 451 272 559 546.6 0 559 273 356 546.6 0 356 274 301 546.6 0 301 275 262 546.6 0 262 276 138 546.6 0 138 277 161 546.6 0 161 278 181 546.6 0 181 279 131 546.6 0 131 280 135 546.6 0 135 281 141 546.6 0 141 282 231 546.6 0 231 283 232 546.6 0 232 284 275 546.6 0 275 285 189 546.6 0 189 286 191 546.6 0 191 287 237 546.6 0 237 288 153 546.6 0 153 289 265 546.6 0 265 290 206 546.6 0 206 291 500 546.6 0 500 292 740 546.6 0 740 293 453 546.6 0 453 294 195 546.6 0 195 295 113 546.6 0 113 296 162 546.6 0 162 297 173 546.6 0 173 298 281 546.6 0 281 299 452 546.6 0 452 300 299 546.6 0 299 301 354 546.6 0 354 Finished in 0.86 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 21,291 Reads with adapters: 627 (2.9%) Reads that were too short: 13 (0.1%) Reads written (passing filters): 614 (2.9%) Total basepairs processed: 4,010,282 bp Total written (filtered): 165,304 bp (4.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 627 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 8 0.0 3 0 7 0 1 20 579 0.0 4 538 32 7 2 21 5 0.0 4 2 3 23 2 0.0 4 2 29 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 11 0.0 4 0 0 0 0 11 128 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 1 157 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 2 185 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 614 Reads with adapters: 131 (21.3%) Reads written (passing filters): 614 (100.0%) Total basepairs processed: 165,304 bp Total written (filtered): 163,561 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 131 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.8% C: 6.9% G: 85.5% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 9.6 0 1 9 2 0.0 0 0 2 10 25 0.0 1 2 23 11 98 0.0 1 3 95 12 2 0.0 1 0 2 73 2 0.0 1 1 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.