This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 60.01 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 20,921 (0.9%) Reads that were too short: 10,023 (0.4%) Reads written (passing filters): 10,898 (0.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 2,201,974 bp (0.3%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 20921 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 115 546.6 0 115 7 601 546.6 0 601 8 675 546.6 0 675 9 29 546.6 0 29 10 23 546.6 0 23 11 23 546.6 0 23 12 21 546.6 0 21 13 25 546.6 0 25 14 28 546.6 0 28 15 31 546.6 0 31 16 23 546.6 0 23 17 32 546.6 0 32 18 24 546.6 0 24 19 30 546.6 0 30 20 26 546.6 0 26 21 18 546.6 0 18 22 27 546.6 0 27 23 20 546.6 0 20 24 37 546.6 0 37 25 32 546.6 0 32 26 27 546.6 0 27 27 23 546.6 0 23 28 29 546.6 0 29 29 39 546.6 0 39 30 29 546.6 0 29 31 30 546.6 0 30 32 149 546.6 0 149 33 31 546.6 0 31 34 33 546.6 0 33 35 28 546.6 0 28 36 31 546.6 0 31 37 36 546.6 0 36 38 22 546.6 0 22 39 32 546.6 0 32 40 28 546.6 0 28 41 36 546.6 0 36 42 24 546.6 0 24 43 48 546.6 0 48 44 44 546.6 0 44 45 43 546.6 0 43 46 37 546.6 0 37 47 55 546.6 0 55 48 33 546.6 0 33 49 25 546.6 0 25 50 40 546.6 0 40 51 28 546.6 0 28 52 22 546.6 0 22 53 31 546.6 0 31 54 58 546.6 0 58 55 33 546.6 0 33 56 35 546.6 0 35 57 26 546.6 0 26 58 23 546.6 0 23 59 37 546.6 0 37 60 20 546.6 0 20 61 19 546.6 0 19 62 29 546.6 0 29 63 84 546.6 0 84 64 612 546.6 0 612 65 61 546.6 0 61 66 274 546.6 0 274 67 113 546.6 0 113 68 24 546.6 0 24 69 31 546.6 0 31 70 37 546.6 0 37 71 39 546.6 0 39 72 22 546.6 0 22 73 26 546.6 0 26 74 16 546.6 0 16 75 26 546.6 0 26 76 26 546.6 0 26 77 22 546.6 0 22 78 26 546.6 0 26 79 33 546.6 0 33 80 46 546.6 0 46 81 46 546.6 0 46 82 15 546.6 0 15 83 47 546.6 0 47 84 33 546.6 0 33 85 22 546.6 0 22 86 31 546.6 0 31 87 30 546.6 0 30 88 31 546.6 0 31 89 40 546.6 0 40 90 47 546.6 0 47 91 59 546.6 0 59 92 359 546.6 0 359 93 111 546.6 0 111 94 117 546.6 0 117 95 21 546.6 0 21 96 28 546.6 0 28 97 15 546.6 0 15 98 29 546.6 0 29 99 31 546.6 0 31 100 29 546.6 0 29 101 31 546.6 0 31 102 33 546.6 0 33 103 34 546.6 0 34 104 29 546.6 0 29 105 22 546.6 0 22 106 42 546.6 0 42 107 26 546.6 0 26 108 31 546.6 0 31 109 39 546.6 0 39 110 40 546.6 0 40 111 37 546.6 0 37 112 20 546.6 0 20 113 20 546.6 0 20 114 30 546.6 0 30 115 28 546.6 0 28 116 29 546.6 0 29 117 41 546.6 0 41 118 34 546.6 0 34 119 23 546.6 0 23 120 39 546.6 0 39 121 35 546.6 0 35 122 38 546.6 0 38 123 37 546.6 0 37 124 49 546.6 0 49 125 29 546.6 0 29 126 28 546.6 0 28 127 23 546.6 0 23 128 34 546.6 0 34 129 52 546.6 0 52 130 35 546.6 0 35 131 57 546.6 0 57 132 48 546.6 0 48 133 38 546.6 0 38 134 27 546.6 0 27 135 37 546.6 0 37 136 119 546.6 0 119 137 70 546.6 0 70 138 40 546.6 0 40 139 39 546.6 0 39 140 41 546.6 0 41 141 27 546.6 0 27 142 38 546.6 0 38 143 40 546.6 0 40 144 30 546.6 0 30 145 31 546.6 0 31 146 24 546.6 0 24 147 57 546.6 0 57 148 62 546.6 0 62 149 53 546.6 0 53 150 72 546.6 0 72 151 41 546.6 0 41 152 51 546.6 0 51 153 65 546.6 0 65 154 33 546.6 0 33 155 52 546.6 0 52 156 81 546.6 0 81 157 93 546.6 0 93 158 124 546.6 0 124 159 48 546.6 0 48 160 65 546.6 0 65 161 30 546.6 0 30 162 44 546.6 0 44 163 39 546.6 0 39 164 46 546.6 0 46 165 44 546.6 0 44 166 60 546.6 0 60 167 162 546.6 0 162 168 67 546.6 0 67 169 100 546.6 0 100 170 31 546.6 0 31 171 75 546.6 0 75 172 81 546.6 0 81 173 110 546.6 0 110 174 38 546.6 0 38 175 129 546.6 0 129 176 105 546.6 0 105 177 125 546.6 0 125 178 46 546.6 0 46 179 40 546.6 0 40 180 32 546.6 0 32 181 48 546.6 0 48 182 39 546.6 0 39 183 43 546.6 0 43 184 38 546.6 0 38 185 36 546.6 0 36 186 56 546.6 0 56 187 51 546.6 0 51 188 168 546.6 0 168 189 69 546.6 0 69 190 56 546.6 0 56 191 50 546.6 0 50 192 40 546.6 0 40 193 49 546.6 0 49 194 38 546.6 0 38 195 31 546.6 0 31 196 49 546.6 0 49 197 45 546.6 0 45 198 49 546.6 0 49 199 50 546.6 0 50 200 49 546.6 0 49 201 37 546.6 0 37 202 52 546.6 0 52 203 59 546.6 0 59 204 69 546.6 0 69 205 53 546.6 0 53 206 50 546.6 0 50 207 50 546.6 0 50 208 55 546.6 0 55 209 43 546.6 0 43 210 47 546.6 0 47 211 63 546.6 0 63 212 66 546.6 0 66 213 56 546.6 0 56 214 57 546.6 0 57 215 76 546.6 0 76 216 57 546.6 0 57 217 70 546.6 0 70 218 57 546.6 0 57 219 62 546.6 0 62 220 73 546.6 0 73 221 104 546.6 0 104 222 142 546.6 0 142 223 197 546.6 0 197 224 158 546.6 0 158 225 122 546.6 0 122 226 105 546.6 0 105 227 66 546.6 0 66 228 85 546.6 0 85 229 60 546.6 0 60 230 33 546.6 0 33 231 37 546.6 0 37 232 49 546.6 0 49 233 50 546.6 0 50 234 73 546.6 0 73 235 64 546.6 0 64 236 73 546.6 0 73 237 80 546.6 0 80 238 94 546.6 0 94 239 68 546.6 0 68 240 56 546.6 0 56 241 187 546.6 0 187 242 122 546.6 0 122 243 282 546.6 0 282 244 233 546.6 0 233 245 162 546.6 0 162 246 75 546.6 0 75 247 73 546.6 0 73 248 67 546.6 0 67 249 150 546.6 0 150 250 69 546.6 0 69 251 73 546.6 0 73 252 84 546.6 0 84 253 220 546.6 0 220 254 364 546.6 0 364 255 66 546.6 0 66 256 73 546.6 0 73 257 54 546.6 0 54 258 39 546.6 0 39 259 29 546.6 0 29 260 40 546.6 0 40 261 44 546.6 0 44 262 63 546.6 0 63 263 62 546.6 0 62 264 96 546.6 0 96 265 79 546.6 0 79 266 91 546.6 0 91 267 58 546.6 0 58 268 131 546.6 0 131 269 175 546.6 0 175 270 569 546.6 0 569 271 141 546.6 0 141 272 142 546.6 0 142 273 131 546.6 0 131 274 103 546.6 0 103 275 109 546.6 0 109 276 84 546.6 0 84 277 76 546.6 0 76 278 141 546.6 0 141 279 78 546.6 0 78 280 83 546.6 0 83 281 71 546.6 0 71 282 84 546.6 0 84 283 96 546.6 0 96 284 104 546.6 0 104 285 95 546.6 0 95 286 61 546.6 0 61 287 110 546.6 0 110 288 70 546.6 0 70 289 80 546.6 0 80 290 82 546.6 0 82 291 144 546.6 0 144 292 183 546.6 0 183 293 155 546.6 0 155 294 58 546.6 0 58 295 72 546.6 0 72 296 75 546.6 0 75 297 140 546.6 0 140 298 139 546.6 0 139 299 162 546.6 0 162 300 148 546.6 0 148 301 145 546.6 0 145 Finished in 0.41 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 10,898 Reads with adapters: 706 (6.5%) Reads that were too short: 42 (0.4%) Reads written (passing filters): 664 (6.1%) Total basepairs processed: 2,201,974 bp Total written (filtered): 181,964 bp (8.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 706 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 2 0.0 3 0 2 17 573 0.0 3 562 10 1 18 2 0.0 3 0 2 19 3 0.0 3 2 0 0 1 20 75 0.0 3 69 1 0 5 23 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 1 153 1 0.0 3 0 1 160 34 0.0 3 0 0 1 33 161 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 173 1 0.0 3 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 664 Reads with adapters: 0 (0.0%) Reads written (passing filters): 664 (100.0%) Total basepairs processed: 181,964 bp Total written (filtered): 181,964 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.