This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.13 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 260,855 (11.7%) Reads that were too short: 225,470 (10.1%) Reads written (passing filters): 35,385 (1.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 8,008,601 bp (1.2%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 260855 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 207 546.6 0 207 7 7347 546.6 0 7347 8 261 546.6 0 261 9 6742 546.6 0 6742 10 82 546.6 0 82 11 73 546.6 0 73 12 54 546.6 0 54 13 60 546.6 0 60 14 78 546.6 0 78 15 76 546.6 0 76 16 50 546.6 0 50 17 54 546.6 0 54 18 61 546.6 0 61 19 56 546.6 0 56 20 67 546.6 0 67 21 51 546.6 0 51 22 44 546.6 0 44 23 58 546.6 0 58 24 61 546.6 0 61 25 68 546.6 0 68 26 61 546.6 0 61 27 88 546.6 0 88 28 78 546.6 0 78 29 79 546.6 0 79 30 90 546.6 0 90 31 83 546.6 0 83 32 74 546.6 0 74 33 70 546.6 0 70 34 59 546.6 0 59 35 84 546.6 0 84 36 154 546.6 0 154 37 206 546.6 0 206 38 195 546.6 0 195 39 54 546.6 0 54 40 63 546.6 0 63 41 47 546.6 0 47 42 38 546.6 0 38 43 59 546.6 0 59 44 54 546.6 0 54 45 54 546.6 0 54 46 52 546.6 0 52 47 61 546.6 0 61 48 40 546.6 0 40 49 46 546.6 0 46 50 43 546.6 0 43 51 29 546.6 0 29 52 43 546.6 0 43 53 49 546.6 0 49 54 50 546.6 0 50 55 26 546.6 0 26 56 299 546.6 0 299 57 88 546.6 0 88 58 41 546.6 0 41 59 45 546.6 0 45 60 53 546.6 0 53 61 46 546.6 0 46 62 43 546.6 0 43 63 29 546.6 0 29 64 44 546.6 0 44 65 49 546.6 0 49 66 52 546.6 0 52 67 66 546.6 0 66 68 48 546.6 0 48 69 84 546.6 0 84 70 69 546.6 0 69 71 148 546.6 0 148 72 61 546.6 0 61 73 96 546.6 0 96 74 59 546.6 0 59 75 89 546.6 0 89 76 87 546.6 0 87 77 91 546.6 0 91 78 88 546.6 0 88 79 67 546.6 0 67 80 60 546.6 0 60 81 82 546.6 0 82 82 81 546.6 0 81 83 96 546.6 0 96 84 69 546.6 0 69 85 79 546.6 0 79 86 78 546.6 0 78 87 62 546.6 0 62 88 89 546.6 0 89 89 47 546.6 0 47 90 53 546.6 0 53 91 48 546.6 0 48 92 55 546.6 0 55 93 50 546.6 0 50 94 65 546.6 0 65 95 65 546.6 0 65 96 140 546.6 0 140 97 82 546.6 0 82 98 66 546.6 0 66 99 103 546.6 0 103 100 73 546.6 0 73 101 66 546.6 0 66 102 70 546.6 0 70 103 73 546.6 0 73 104 78 546.6 0 78 105 62 546.6 0 62 106 78 546.6 0 78 107 91 546.6 0 91 108 81 546.6 0 81 109 77 546.6 0 77 110 96 546.6 0 96 111 95 546.6 0 95 112 112 546.6 0 112 113 77 546.6 0 77 114 79 546.6 0 79 115 104 546.6 0 104 116 80 546.6 0 80 117 58 546.6 0 58 118 508 546.6 0 508 119 83 546.6 0 83 120 132 546.6 0 132 121 92 546.6 0 92 122 121 546.6 0 121 123 157 546.6 0 157 124 202 546.6 0 202 125 116 546.6 0 116 126 99 546.6 0 99 127 75 546.6 0 75 128 94 546.6 0 94 129 64 546.6 0 64 130 62 546.6 0 62 131 114 546.6 0 114 132 81 546.6 0 81 133 109 546.6 0 109 134 92 546.6 0 92 135 78 546.6 0 78 136 85 546.6 0 85 137 78 546.6 0 78 138 133 546.6 0 133 139 221 546.6 0 221 140 205 546.6 0 205 141 157 546.6 0 157 142 75 546.6 0 75 143 74 546.6 0 74 144 70 546.6 0 70 145 91 546.6 0 91 146 69 546.6 0 69 147 90 546.6 0 90 148 100 546.6 0 100 149 54 546.6 0 54 150 55 546.6 0 55 151 37 546.6 0 37 152 39 546.6 0 39 153 62 546.6 0 62 154 76 546.6 0 76 155 101 546.6 0 101 156 96 546.6 0 96 157 119 546.6 0 119 158 240 546.6 0 240 159 2067 546.6 0 2067 160 1281 546.6 0 1281 161 2396 546.6 0 2396 162 85 546.6 0 85 163 68 546.6 0 68 164 45 546.6 0 45 165 72 546.6 0 72 166 69 546.6 0 69 167 48 546.6 0 48 168 66 546.6 0 66 169 53 546.6 0 53 170 60 546.6 0 60 171 45 546.6 0 45 172 58 546.6 0 58 173 75 546.6 0 75 174 74 546.6 0 74 175 117 546.6 0 117 176 84 546.6 0 84 177 59 546.6 0 59 178 40 546.6 0 40 179 67 546.6 0 67 180 59 546.6 0 59 181 95 546.6 0 95 182 60 546.6 0 60 183 48 546.6 0 48 184 59 546.6 0 59 185 73 546.6 0 73 186 51 546.6 0 51 187 76 546.6 0 76 188 48 546.6 0 48 189 55 546.6 0 55 190 48 546.6 0 48 191 43 546.6 0 43 192 66 546.6 0 66 193 77 546.6 0 77 194 70 546.6 0 70 195 59 546.6 0 59 196 52 546.6 0 52 197 63 546.6 0 63 198 100 546.6 0 100 199 90 546.6 0 90 200 66 546.6 0 66 201 51 546.6 0 51 202 70 546.6 0 70 203 68 546.6 0 68 204 62 546.6 0 62 205 90 546.6 0 90 206 121 546.6 0 121 207 217 546.6 0 217 208 172 546.6 0 172 209 354 546.6 0 354 210 194 546.6 0 194 211 240 546.6 0 240 212 215 546.6 0 215 213 285 546.6 0 285 214 229 546.6 0 229 215 69 546.6 0 69 216 80 546.6 0 80 217 101 546.6 0 101 218 302 546.6 0 302 219 145 546.6 0 145 220 95 546.6 0 95 221 142 546.6 0 142 222 121 546.6 0 121 223 114 546.6 0 114 224 119 546.6 0 119 225 224 546.6 0 224 226 85 546.6 0 85 227 174 546.6 0 174 228 437 546.6 0 437 229 202 546.6 0 202 230 110 546.6 0 110 231 127 546.6 0 127 232 1011 546.6 0 1011 233 341 546.6 0 341 234 1310 546.6 0 1310 235 208 546.6 0 208 236 146 546.6 0 146 237 256 546.6 0 256 238 628 546.6 0 628 239 164 546.6 0 164 240 266 546.6 0 266 241 168 546.6 0 168 242 204 546.6 0 204 243 260 546.6 0 260 244 149 546.6 0 149 245 118 546.6 0 118 246 79 546.6 0 79 247 118 546.6 0 118 248 271 546.6 0 271 249 299 546.6 0 299 250 576 546.6 0 576 251 923 546.6 0 923 252 1298 546.6 0 1298 253 2874 546.6 0 2874 254 11279 546.6 0 11279 255 99457 546.6 0 99457 256 56877 546.6 0 56877 257 36740 546.6 0 36740 258 1480 546.6 0 1480 259 98 546.6 0 98 260 189 546.6 0 189 261 121 546.6 0 121 262 128 546.6 0 128 263 82 546.6 0 82 264 78 546.6 0 78 265 62 546.6 0 62 266 115 546.6 0 115 267 243 546.6 0 243 268 172 546.6 0 172 269 35 546.6 0 35 270 33 546.6 0 33 271 48 546.6 0 48 272 39 546.6 0 39 273 59 546.6 0 59 274 55 546.6 0 55 275 53 546.6 0 53 276 48 546.6 0 48 277 39 546.6 0 39 278 34 546.6 0 34 279 63 546.6 0 63 280 61 546.6 0 61 281 49 546.6 0 49 282 52 546.6 0 52 283 51 546.6 0 51 284 72 546.6 0 72 285 32 546.6 0 32 286 37 546.6 0 37 287 36 546.6 0 36 288 44 546.6 0 44 289 29 546.6 0 29 290 49 546.6 0 49 291 46 546.6 0 46 292 44 546.6 0 44 293 43 546.6 0 43 294 54 546.6 0 54 295 65 546.6 0 65 296 49 546.6 0 49 297 48 546.6 0 48 298 45 546.6 0 45 299 74 546.6 0 74 300 114 546.6 0 114 301 118 546.6 0 118 Finished in 1.62 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 35,385 Reads with adapters: 7,392 (20.9%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 7,379 (20.9%) Total basepairs processed: 8,008,601 bp Total written (filtered): 2,018,140 bp (25.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7392 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 1 19 48 0.0 3 0 41 4 1 2 20 7256 0.0 4 6892 289 55 16 4 21 30 0.0 4 3 21 3 2 1 22 2 0.0 4 1 0 1 23 9 0.0 4 0 0 0 7 2 24 1 0.0 4 0 0 1 25 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 1 58 1 0.0 4 0 0 0 0 1 96 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 127 24 0.0 4 0 0 0 0 24 129 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 1 265 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 7,379 Reads with adapters: 747 (10.1%) Reads written (passing filters): 7,379 (100.0%) Total basepairs processed: 2,018,140 bp Total written (filtered): 2,006,849 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 747 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.1% C: 9.4% G: 81.5% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 115.3 0 25 4 3 28.8 0 3 9 7 0.0 0 1 6 10 8 0.0 1 2 6 11 602 0.0 1 36 566 12 78 0.0 1 2 76 13 4 0.0 1 0 4 14 3 0.0 1 0 3 152 3 0.0 1 1 2 203 1 0.0 1 1 210 12 0.0 1 7 5 230 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.