This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.26 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 124,050 (5.5%) Reads that were too short: 81,646 (3.6%) Reads written (passing filters): 42,404 (1.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,486,753 bp (1.4%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 124050 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 287 546.6 0 287 7 4247 546.6 0 4247 8 3411 546.6 0 3411 9 14 546.6 0 14 10 23 546.6 0 23 11 12 546.6 0 12 12 27 546.6 0 27 13 19 546.6 0 19 14 13 546.6 0 13 15 25 546.6 0 25 16 12 546.6 0 12 17 18 546.6 0 18 18 19 546.6 0 19 19 22 546.6 0 22 20 27 546.6 0 27 21 39 546.6 0 39 22 14 546.6 0 14 23 7 546.6 0 7 24 27 546.6 0 27 25 18 546.6 0 18 26 11 546.6 0 11 27 14 546.6 0 14 28 16 546.6 0 16 29 40 546.6 0 40 30 84 546.6 0 84 31 24 546.6 0 24 32 15 546.6 0 15 33 17 546.6 0 17 34 11 546.6 0 11 35 18 546.6 0 18 36 7 546.6 0 7 37 20 546.6 0 20 38 19 546.6 0 19 39 18 546.6 0 18 40 30 546.6 0 30 41 17 546.6 0 17 42 20 546.6 0 20 43 14 546.6 0 14 44 14 546.6 0 14 45 14 546.6 0 14 46 25 546.6 0 25 47 38 546.6 0 38 48 32 546.6 0 32 49 45 546.6 0 45 50 72 546.6 0 72 51 64 546.6 0 64 52 37 546.6 0 37 53 21 546.6 0 21 54 34 546.6 0 34 55 43 546.6 0 43 56 46 546.6 0 46 57 47 546.6 0 47 58 45 546.6 0 45 59 46 546.6 0 46 60 78 546.6 0 78 61 93 546.6 0 93 62 179 546.6 0 179 63 76 546.6 0 76 64 85 546.6 0 85 65 151 546.6 0 151 66 53 546.6 0 53 67 50 546.6 0 50 68 121 546.6 0 121 69 111 546.6 0 111 70 164 546.6 0 164 71 174 546.6 0 174 72 200 546.6 0 200 73 388 546.6 0 388 74 476 546.6 0 476 75 5674 546.6 0 5674 76 5938 546.6 0 5938 77 11355 546.6 0 11355 78 59 546.6 0 59 79 31 546.6 0 31 80 22 546.6 0 22 81 19 546.6 0 19 82 22 546.6 0 22 83 19 546.6 0 19 84 26 546.6 0 26 85 9 546.6 0 9 86 21 546.6 0 21 87 12 546.6 0 12 88 26 546.6 0 26 89 15 546.6 0 15 90 35 546.6 0 35 91 23 546.6 0 23 92 32 546.6 0 32 93 11 546.6 0 11 94 21 546.6 0 21 95 27 546.6 0 27 96 11 546.6 0 11 97 12 546.6 0 12 98 17 546.6 0 17 99 8 546.6 0 8 100 9 546.6 0 9 101 11 546.6 0 11 102 10 546.6 0 10 103 13 546.6 0 13 104 7 546.6 0 7 105 7 546.6 0 7 106 15 546.6 0 15 107 11 546.6 0 11 108 11 546.6 0 11 109 11 546.6 0 11 110 19 546.6 0 19 111 16 546.6 0 16 112 18 546.6 0 18 113 15 546.6 0 15 114 18 546.6 0 18 115 30 546.6 0 30 116 26 546.6 0 26 117 31 546.6 0 31 118 22 546.6 0 22 119 22 546.6 0 22 120 39 546.6 0 39 121 205 546.6 0 205 122 23 546.6 0 23 123 29 546.6 0 29 124 17 546.6 0 17 125 36 546.6 0 36 126 90 546.6 0 90 127 738 546.6 0 738 128 55 546.6 0 55 129 45 546.6 0 45 130 57 546.6 0 57 131 52 546.6 0 52 132 28 546.6 0 28 133 34 546.6 0 34 134 35 546.6 0 35 135 110 546.6 0 110 136 110 546.6 0 110 137 180 546.6 0 180 138 28 546.6 0 28 139 21 546.6 0 21 140 19 546.6 0 19 141 16 546.6 0 16 142 44 546.6 0 44 143 49 546.6 0 49 144 55 546.6 0 55 145 51 546.6 0 51 146 10 546.6 0 10 147 15 546.6 0 15 148 20 546.6 0 20 149 22 546.6 0 22 150 22 546.6 0 22 151 14 546.6 0 14 152 14 546.6 0 14 153 24 546.6 0 24 154 18 546.6 0 18 155 16 546.6 0 16 156 25 546.6 0 25 157 40 546.6 0 40 158 22 546.6 0 22 159 14 546.6 0 14 160 12 546.6 0 12 161 23 546.6 0 23 162 30 546.6 0 30 163 59 546.6 0 59 164 43 546.6 0 43 165 37 546.6 0 37 166 56 546.6 0 56 167 73 546.6 0 73 168 152 546.6 0 152 169 89 546.6 0 89 170 79 546.6 0 79 171 42 546.6 0 42 172 15 546.6 0 15 173 18 546.6 0 18 174 15 546.6 0 15 175 27 546.6 0 27 176 32 546.6 0 32 177 22 546.6 0 22 178 23 546.6 0 23 179 37 546.6 0 37 180 62 546.6 0 62 181 45 546.6 0 45 182 64 546.6 0 64 183 53 546.6 0 53 184 59 546.6 0 59 185 51 546.6 0 51 186 216 546.6 0 216 187 155 546.6 0 155 188 346 546.6 0 346 189 156 546.6 0 156 190 58 546.6 0 58 191 45 546.6 0 45 192 77 546.6 0 77 193 182 546.6 0 182 194 204 546.6 0 204 195 296 546.6 0 296 196 145 546.6 0 145 197 310 546.6 0 310 198 303 546.6 0 303 199 406 546.6 0 406 200 132 546.6 0 132 201 248 546.6 0 248 202 176 546.6 0 176 203 466 546.6 0 466 204 343 546.6 0 343 205 281 546.6 0 281 206 58 546.6 0 58 207 33 546.6 0 33 208 46 546.6 0 46 209 64 546.6 0 64 210 78 546.6 0 78 211 74 546.6 0 74 212 43 546.6 0 43 213 82 546.6 0 82 214 140 546.6 0 140 215 194 546.6 0 194 216 122 546.6 0 122 217 118 546.6 0 118 218 185 546.6 0 185 219 294 546.6 0 294 220 213 546.6 0 213 221 75 546.6 0 75 222 108 546.6 0 108 223 231 546.6 0 231 224 433 546.6 0 433 225 278 546.6 0 278 226 166 546.6 0 166 227 91 546.6 0 91 228 66 546.6 0 66 229 132 546.6 0 132 230 114 546.6 0 114 231 99 546.6 0 99 232 96 546.6 0 96 233 156 546.6 0 156 234 241 546.6 0 241 235 342 546.6 0 342 236 435 546.6 0 435 237 815 546.6 0 815 238 7158 546.6 0 7158 239 2209 546.6 0 2209 240 7472 546.6 0 7472 241 268 546.6 0 268 242 160 546.6 0 160 243 195 546.6 0 195 244 282 546.6 0 282 245 261 546.6 0 261 246 516 546.6 0 516 247 580 546.6 0 580 248 4038 546.6 0 4038 249 18643 546.6 0 18643 250 13967 546.6 0 13967 251 6150 546.6 0 6150 252 185 546.6 0 185 253 182 546.6 0 182 254 181 546.6 0 181 255 98 546.6 0 98 256 108 546.6 0 108 257 123 546.6 0 123 258 50 546.6 0 50 259 118 546.6 0 118 260 51 546.6 0 51 261 79 546.6 0 79 262 65 546.6 0 65 263 167 546.6 0 167 264 67 546.6 0 67 265 60 546.6 0 60 266 73 546.6 0 73 267 55 546.6 0 55 268 79 546.6 0 79 269 107 546.6 0 107 270 116 546.6 0 116 271 150 546.6 0 150 272 69 546.6 0 69 273 89 546.6 0 89 274 66 546.6 0 66 275 77 546.6 0 77 276 67 546.6 0 67 277 95 546.6 0 95 278 143 546.6 0 143 279 195 546.6 0 195 280 285 546.6 0 285 281 271 546.6 0 271 282 538 546.6 0 538 283 863 546.6 0 863 284 2266 546.6 0 2266 285 963 546.6 0 963 286 255 546.6 0 255 287 263 546.6 0 263 288 277 546.6 0 277 289 147 546.6 0 147 290 117 546.6 0 117 291 176 546.6 0 176 292 173 546.6 0 173 293 166 546.6 0 166 294 218 546.6 0 218 295 387 546.6 0 387 296 690 546.6 0 690 297 570 546.6 0 570 298 403 546.6 0 403 299 307 546.6 0 307 300 278 546.6 0 278 301 331 546.6 0 331 Finished in 1.90 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 42,404 Reads with adapters: 3,860 (9.1%) Reads that were too short: 27 (0.1%) Reads written (passing filters): 3,833 (9.0%) Total basepairs processed: 9,486,753 bp Total written (filtered): 1,040,934 bp (11.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3860 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 1 1 19 64 0.0 3 0 56 2 6 20 3525 0.0 4 3289 192 34 7 3 21 17 0.0 4 5 10 1 0 1 22 161 0.0 4 136 13 6 2 4 23 11 0.0 4 0 1 0 6 4 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 1 26 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 59 23 0.0 4 0 0 0 0 23 62 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 1 72 3 0.0 4 0 0 0 0 3 73 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 85 4 0.0 4 0 0 0 0 4 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 12 0.0 4 0 0 0 0 12 128 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 1 158 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 193 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 1 261 1 0.0 4 0 1 262 1 0.0 4 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 3,833 Reads with adapters: 724 (18.9%) Reads written (passing filters): 3,833 (100.0%) Total basepairs processed: 1,040,934 bp Total written (filtered): 1,032,389 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 724 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 10.2% G: 84.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 59.9 0 6 4 2 15.0 0 2 9 2 0.0 0 0 2 10 265 0.0 1 22 243 11 434 0.0 1 19 415 12 6 0.0 1 0 6 13 3 0.0 1 0 3 151 4 0.0 1 3 1 153 1 0.0 1 0 1 209 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.