This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.00 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 123,060 (5.5%) Reads that were too short: 92,825 (4.1%) Reads written (passing filters): 30,235 (1.4%) Total basepairs processed: 673,916,425 bp Total written (filtered): 7,087,107 bp (1.1%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 123060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 303 546.6 0 303 7 3924 546.6 0 3924 8 3991 546.6 0 3991 9 19 546.6 0 19 10 17 546.6 0 17 11 15 546.6 0 15 12 13 546.6 0 13 13 20 546.6 0 20 14 19 546.6 0 19 15 21 546.6 0 21 16 10 546.6 0 10 17 13 546.6 0 13 18 16 546.6 0 16 19 15 546.6 0 15 20 15 546.6 0 15 21 26 546.6 0 26 22 16 546.6 0 16 23 13 546.6 0 13 24 17 546.6 0 17 25 13 546.6 0 13 26 11 546.6 0 11 27 11 546.6 0 11 28 16 546.6 0 16 29 16 546.6 0 16 30 17 546.6 0 17 31 12 546.6 0 12 32 10 546.6 0 10 33 11 546.6 0 11 34 12 546.6 0 12 35 20 546.6 0 20 36 10 546.6 0 10 37 6 546.6 0 6 38 13 546.6 0 13 39 19 546.6 0 19 40 22 546.6 0 22 41 11 546.6 0 11 42 23 546.6 0 23 43 19 546.6 0 19 44 13 546.6 0 13 45 8 546.6 0 8 46 10 546.6 0 10 47 11 546.6 0 11 48 25 546.6 0 25 49 21 546.6 0 21 50 27 546.6 0 27 51 27 546.6 0 27 52 19 546.6 0 19 53 24 546.6 0 24 54 31 546.6 0 31 55 32 546.6 0 32 56 44 546.6 0 44 57 41 546.6 0 41 58 26 546.6 0 26 59 33 546.6 0 33 60 45 546.6 0 45 61 62 546.6 0 62 62 156 546.6 0 156 63 60 546.6 0 60 64 59 546.6 0 59 65 122 546.6 0 122 66 36 546.6 0 36 67 37 546.6 0 37 68 75 546.6 0 75 69 63 546.6 0 63 70 78 546.6 0 78 71 86 546.6 0 86 72 91 546.6 0 91 73 153 546.6 0 153 74 160 546.6 0 160 75 2975 546.6 0 2975 76 3591 546.6 0 3591 77 8997 546.6 0 8997 78 37 546.6 0 37 79 15 546.6 0 15 80 15 546.6 0 15 81 17 546.6 0 17 82 16 546.6 0 16 83 29 546.6 0 29 84 11 546.6 0 11 85 11 546.6 0 11 86 9 546.6 0 9 87 17 546.6 0 17 88 15 546.6 0 15 89 12 546.6 0 12 90 12 546.6 0 12 91 30 546.6 0 30 92 19 546.6 0 19 93 5 546.6 0 5 94 16 546.6 0 16 95 30 546.6 0 30 96 25 546.6 0 25 97 15 546.6 0 15 98 21 546.6 0 21 99 9 546.6 0 9 100 12 546.6 0 12 101 16 546.6 0 16 102 8 546.6 0 8 103 20 546.6 0 20 104 17 546.6 0 17 105 17 546.6 0 17 106 6 546.6 0 6 107 11 546.6 0 11 108 12 546.6 0 12 109 7 546.6 0 7 110 12 546.6 0 12 111 15 546.6 0 15 112 21 546.6 0 21 113 12 546.6 0 12 114 18 546.6 0 18 115 21 546.6 0 21 116 20 546.6 0 20 117 15 546.6 0 15 118 19 546.6 0 19 119 18 546.6 0 18 120 29 546.6 0 29 121 60 546.6 0 60 122 17 546.6 0 17 123 28 546.6 0 28 124 21 546.6 0 21 125 29 546.6 0 29 126 70 546.6 0 70 127 635 546.6 0 635 128 31 546.6 0 31 129 17 546.6 0 17 130 10 546.6 0 10 131 12 546.6 0 12 132 11 546.6 0 11 133 17 546.6 0 17 134 12 546.6 0 12 135 72 546.6 0 72 136 44 546.6 0 44 137 118 546.6 0 118 138 21 546.6 0 21 139 19 546.6 0 19 140 23 546.6 0 23 141 18 546.6 0 18 142 30 546.6 0 30 143 26 546.6 0 26 144 34 546.6 0 34 145 37 546.6 0 37 146 15 546.6 0 15 147 14 546.6 0 14 148 15 546.6 0 15 149 16 546.6 0 16 150 27 546.6 0 27 151 9 546.6 0 9 152 8 546.6 0 8 153 15 546.6 0 15 154 18 546.6 0 18 155 9 546.6 0 9 156 8 546.6 0 8 157 31 546.6 0 31 158 25 546.6 0 25 159 19 546.6 0 19 160 17 546.6 0 17 161 17 546.6 0 17 162 23 546.6 0 23 163 24 546.6 0 24 164 30 546.6 0 30 165 28 546.6 0 28 166 30 546.6 0 30 167 31 546.6 0 31 168 50 546.6 0 50 169 26 546.6 0 26 170 29 546.6 0 29 171 26 546.6 0 26 172 13 546.6 0 13 173 16 546.6 0 16 174 15 546.6 0 15 175 19 546.6 0 19 176 25 546.6 0 25 177 22 546.6 0 22 178 20 546.6 0 20 179 33 546.6 0 33 180 47 546.6 0 47 181 35 546.6 0 35 182 47 546.6 0 47 183 36 546.6 0 36 184 27 546.6 0 27 185 28 546.6 0 28 186 73 546.6 0 73 187 59 546.6 0 59 188 92 546.6 0 92 189 49 546.6 0 49 190 30 546.6 0 30 191 36 546.6 0 36 192 34 546.6 0 34 193 61 546.6 0 61 194 65 546.6 0 65 195 65 546.6 0 65 196 43 546.6 0 43 197 123 546.6 0 123 198 139 546.6 0 139 199 150 546.6 0 150 200 51 546.6 0 51 201 105 546.6 0 105 202 80 546.6 0 80 203 95 546.6 0 95 204 101 546.6 0 101 205 75 546.6 0 75 206 33 546.6 0 33 207 35 546.6 0 35 208 29 546.6 0 29 209 21 546.6 0 21 210 40 546.6 0 40 211 66 546.6 0 66 212 33 546.6 0 33 213 39 546.6 0 39 214 51 546.6 0 51 215 52 546.6 0 52 216 56 546.6 0 56 217 52 546.6 0 52 218 125 546.6 0 125 219 165 546.6 0 165 220 139 546.6 0 139 221 54 546.6 0 54 222 63 546.6 0 63 223 243 546.6 0 243 224 398 546.6 0 398 225 226 546.6 0 226 226 106 546.6 0 106 227 39 546.6 0 39 228 45 546.6 0 45 229 183 546.6 0 183 230 141 546.6 0 141 231 82 546.6 0 82 232 90 546.6 0 90 233 137 546.6 0 137 234 229 546.6 0 229 235 348 546.6 0 348 236 460 546.6 0 460 237 842 546.6 0 842 238 9787 546.6 0 9787 239 2941 546.6 0 2941 240 10463 546.6 0 10463 241 295 546.6 0 295 242 155 546.6 0 155 243 166 546.6 0 166 244 254 546.6 0 254 245 269 546.6 0 269 246 398 546.6 0 398 247 490 546.6 0 490 248 4226 546.6 0 4226 249 24726 546.6 0 24726 250 17119 546.6 0 17119 251 7936 546.6 0 7936 252 56 546.6 0 56 253 26 546.6 0 26 254 21 546.6 0 21 255 19 546.6 0 19 256 27 546.6 0 27 257 26 546.6 0 26 258 20 546.6 0 20 259 91 546.6 0 91 260 30 546.6 0 30 261 25 546.6 0 25 262 12 546.6 0 12 263 112 546.6 0 112 264 60 546.6 0 60 265 24 546.6 0 24 266 40 546.6 0 40 267 24 546.6 0 24 268 30 546.6 0 30 269 47 546.6 0 47 270 41 546.6 0 41 271 63 546.6 0 63 272 33 546.6 0 33 273 42 546.6 0 42 274 22 546.6 0 22 275 48 546.6 0 48 276 20 546.6 0 20 277 37 546.6 0 37 278 67 546.6 0 67 279 106 546.6 0 106 280 173 546.6 0 173 281 200 546.6 0 200 282 433 546.6 0 433 283 885 546.6 0 885 284 2422 546.6 0 2422 285 408 546.6 0 408 286 79 546.6 0 79 287 73 546.6 0 73 288 94 546.6 0 94 289 45 546.6 0 45 290 32 546.6 0 32 291 72 546.6 0 72 292 46 546.6 0 46 293 56 546.6 0 56 294 204 546.6 0 204 295 444 546.6 0 444 296 694 546.6 0 694 297 378 546.6 0 378 298 89 546.6 0 89 299 145 546.6 0 145 300 240 546.6 0 240 301 246 546.6 0 246 Finished in 1.25 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 30,235 Reads with adapters: 3,997 (13.2%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 3,958 (13.1%) Total basepairs processed: 7,087,107 bp Total written (filtered): 1,059,002 bp (14.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3997 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 32 0.0 3 0 31 1 17 3671 0.0 3 3590 76 5 18 10 0.0 3 2 7 1 19 3 0.0 3 2 0 1 20 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 1 51 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 1 63 1 0.0 3 0 0 0 1 66 6 0.0 3 0 0 0 6 68 1 0.0 3 0 0 0 1 75 6 0.0 3 0 0 0 6 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 12 0.0 3 0 0 0 12 92 4 0.0 3 0 0 0 4 93 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 0 4 96 28 0.0 3 0 0 0 28 98 14 0.0 3 0 0 0 14 99 14 0.0 3 0 0 1 13 100 6 0.0 3 0 0 0 6 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 13 0.0 3 0 0 0 13 110 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 116 80 0.0 3 0 0 0 80 117 21 0.0 3 0 0 0 21 118 1 0.0 3 0 0 0 1 119 7 0.0 3 0 0 0 7 120 3 0.0 3 0 0 0 3 122 2 0.0 3 0 0 0 2 143 1 0.0 3 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 176 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 203 9 0.0 3 0 0 0 9 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 217 3 0.0 3 0 0 0 3 218 2 0.0 3 0 0 1 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 3,958 Reads with adapters: 16 (0.4%) Reads written (passing filters): 3,958 (100.0%) Total basepairs processed: 1,059,002 bp Total written (filtered): 1,058,952 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 16 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 6.2% C: 31.2% G: 56.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 61.8 0 14 4 2 15.5 0 2