This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 135.23 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 163,004 (5.5%) Reads that were too short: 41,331 (1.4%) Reads written (passing filters): 121,673 (4.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 21,719,761 bp (2.4%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 163004 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 638 720.2 0 638 7 15973 720.2 0 15973 8 352 720.2 0 352 9 16850 720.2 0 16850 10 38 720.2 0 38 11 15 720.2 0 15 12 18 720.2 0 18 13 12 720.2 0 12 14 12 720.2 0 12 15 15 720.2 0 15 16 17 720.2 0 17 17 17 720.2 0 17 18 10 720.2 0 10 19 9 720.2 0 9 20 28 720.2 0 28 21 11 720.2 0 11 22 11 720.2 0 11 23 19 720.2 0 19 24 9 720.2 0 9 25 16 720.2 0 16 26 15 720.2 0 15 27 22 720.2 0 22 28 15 720.2 0 15 29 25 720.2 0 25 30 9 720.2 0 9 31 13 720.2 0 13 32 23 720.2 0 23 33 30 720.2 0 30 34 21 720.2 0 21 35 18 720.2 0 18 36 34 720.2 0 34 37 37 720.2 0 37 38 20 720.2 0 20 39 21 720.2 0 21 40 12 720.2 0 12 41 19 720.2 0 19 42 175 720.2 0 175 43 28 720.2 0 28 44 14 720.2 0 14 45 12 720.2 0 12 46 20 720.2 0 20 47 21 720.2 0 21 48 15 720.2 0 15 49 12 720.2 0 12 50 22 720.2 0 22 51 18 720.2 0 18 52 13 720.2 0 13 53 8 720.2 0 8 54 28 720.2 0 28 55 31 720.2 0 31 56 51 720.2 0 51 57 15 720.2 0 15 58 20 720.2 0 20 59 39 720.2 0 39 60 23 720.2 0 23 61 17 720.2 0 17 62 24 720.2 0 24 63 32 720.2 0 32 64 93 720.2 0 93 65 157 720.2 0 157 66 126 720.2 0 126 67 11 720.2 0 11 68 22 720.2 0 22 69 41 720.2 0 41 70 30 720.2 0 30 71 87 720.2 0 87 72 108 720.2 0 108 73 113 720.2 0 113 74 197 720.2 0 197 75 160 720.2 0 160 76 248 720.2 0 248 77 27 720.2 0 27 78 60 720.2 0 60 79 83 720.2 0 83 80 174 720.2 0 174 81 87 720.2 0 87 82 64 720.2 0 64 83 77 720.2 0 77 84 490 720.2 0 490 85 143 720.2 0 143 86 18 720.2 0 18 87 27 720.2 0 27 88 49 720.2 0 49 89 31 720.2 0 31 90 32 720.2 0 32 91 46 720.2 0 46 92 24 720.2 0 24 93 18 720.2 0 18 94 16 720.2 0 16 95 36 720.2 0 36 96 22 720.2 0 22 97 31 720.2 0 31 98 33 720.2 0 33 99 24 720.2 0 24 100 21 720.2 0 21 101 55 720.2 0 55 102 59 720.2 0 59 103 75 720.2 0 75 104 122 720.2 0 122 105 134 720.2 0 134 106 148 720.2 0 148 107 1656 720.2 0 1656 108 346 720.2 0 346 109 1809 720.2 0 1809 110 68 720.2 0 68 111 102 720.2 0 102 112 76 720.2 0 76 113 94 720.2 0 94 114 66 720.2 0 66 115 106 720.2 0 106 116 137 720.2 0 137 117 222 720.2 0 222 118 116 720.2 0 116 119 145 720.2 0 145 120 406 720.2 0 406 121 791 720.2 0 791 122 881 720.2 0 881 123 223 720.2 0 223 124 279 720.2 0 279 125 347 720.2 0 347 126 241 720.2 0 241 127 139 720.2 0 139 128 118 720.2 0 118 129 59 720.2 0 59 130 99 720.2 0 99 131 94 720.2 0 94 132 75 720.2 0 75 133 66 720.2 0 66 134 58 720.2 0 58 135 89 720.2 0 89 136 86 720.2 0 86 137 109 720.2 0 109 138 110 720.2 0 110 139 124 720.2 0 124 140 65 720.2 0 65 141 127 720.2 0 127 142 91 720.2 0 91 143 145 720.2 0 145 144 96 720.2 0 96 145 85 720.2 0 85 146 190 720.2 0 190 147 143 720.2 0 143 148 113 720.2 0 113 149 103 720.2 0 103 150 90 720.2 0 90 151 98 720.2 0 98 152 96 720.2 0 96 153 97 720.2 0 97 154 90 720.2 0 90 155 91 720.2 0 91 156 77 720.2 0 77 157 133 720.2 0 133 158 71 720.2 0 71 159 109 720.2 0 109 160 154 720.2 0 154 161 110 720.2 0 110 162 110 720.2 0 110 163 119 720.2 0 119 164 217 720.2 0 217 165 215 720.2 0 215 166 1297 720.2 0 1297 167 1031 720.2 0 1031 168 3194 720.2 0 3194 169 283 720.2 0 283 170 1525 720.2 0 1525 171 2392 720.2 0 2392 172 1763 720.2 0 1763 173 219 720.2 0 219 174 160 720.2 0 160 175 193 720.2 0 193 176 249 720.2 0 249 177 358 720.2 0 358 178 347 720.2 0 347 179 463 720.2 0 463 180 763 720.2 0 763 181 17406 720.2 0 17406 182 29258 720.2 0 29258 183 4035 720.2 0 4035 184 1423 720.2 0 1423 185 1131 720.2 0 1131 186 805 720.2 0 805 187 80 720.2 0 80 188 76 720.2 0 76 189 97 720.2 0 97 190 90 720.2 0 90 191 81 720.2 0 81 192 95 720.2 0 95 193 67 720.2 0 67 194 79 720.2 0 79 195 79 720.2 0 79 196 118 720.2 0 118 197 100 720.2 0 100 198 317 720.2 0 317 199 328 720.2 0 328 200 517 720.2 0 517 201 221 720.2 0 221 202 117 720.2 0 117 203 95 720.2 0 95 204 260 720.2 0 260 205 656 720.2 0 656 206 646 720.2 0 646 207 549 720.2 0 549 208 123 720.2 0 123 209 153 720.2 0 153 210 167 720.2 0 167 211 226 720.2 0 226 212 278 720.2 0 278 213 3371 720.2 0 3371 214 2213 720.2 0 2213 215 7838 720.2 0 7838 216 339 720.2 0 339 217 94 720.2 0 94 218 162 720.2 0 162 219 152 720.2 0 152 220 145 720.2 0 145 221 111 720.2 0 111 222 91 720.2 0 91 223 101 720.2 0 101 224 110 720.2 0 110 225 471 720.2 0 471 226 287 720.2 0 287 227 463 720.2 0 463 228 419 720.2 0 419 229 454 720.2 0 454 230 660 720.2 0 660 231 79 720.2 0 79 232 66 720.2 0 66 233 64 720.2 0 64 234 60 720.2 0 60 235 48 720.2 0 48 236 73 720.2 0 73 237 83 720.2 0 83 238 98 720.2 0 98 239 182 720.2 0 182 240 113 720.2 0 113 241 108 720.2 0 108 242 178 720.2 0 178 243 249 720.2 0 249 244 175 720.2 0 175 245 227 720.2 0 227 246 319 720.2 0 319 247 277 720.2 0 277 248 197 720.2 0 197 249 122 720.2 0 122 250 121 720.2 0 121 251 114 720.2 0 114 252 113 720.2 0 113 253 142 720.2 0 142 254 189 720.2 0 189 255 228 720.2 0 228 256 210 720.2 0 210 257 139 720.2 0 139 258 111 720.2 0 111 259 89 720.2 0 89 260 145 720.2 0 145 261 168 720.2 0 168 262 307 720.2 0 307 263 463 720.2 0 463 264 384 720.2 0 384 265 218 720.2 0 218 266 256 720.2 0 256 267 2517 720.2 0 2517 268 2739 720.2 0 2739 269 2889 720.2 0 2889 270 510 720.2 0 510 271 81 720.2 0 81 272 66 720.2 0 66 273 105 720.2 0 105 274 97 720.2 0 97 275 154 720.2 0 154 276 156 720.2 0 156 277 201 720.2 0 201 278 173 720.2 0 173 279 196 720.2 0 196 280 755 720.2 0 755 281 984 720.2 0 984 282 669 720.2 0 669 283 128 720.2 0 128 284 83 720.2 0 83 285 71 720.2 0 71 286 96 720.2 0 96 287 115 720.2 0 115 288 113 720.2 0 113 289 110 720.2 0 110 290 106 720.2 0 106 291 81 720.2 0 81 292 138 720.2 0 138 293 130 720.2 0 130 294 115 720.2 0 115 295 102 720.2 0 102 296 100 720.2 0 100 297 86 720.2 0 86 298 120 720.2 0 120 299 134 720.2 0 134 300 128 720.2 0 128 301 217 720.2 0 217 Finished in 7.48 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 121,673 Reads with adapters: 16,481 (13.5%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 16,443 (13.5%) Total basepairs processed: 21,719,761 bp Total written (filtered): 4,481,662 bp (20.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16481 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 11 0.0 3 0 0 9 2 19 163 0.0 3 0 145 9 7 2 20 15976 0.0 4 15123 704 105 31 13 21 48 0.0 4 11 35 0 2 22 3 0.0 4 0 0 2 1 23 19 0.0 4 1 0 1 12 5 24 2 0.0 4 1 0 0 0 1 26 1 0.0 4 0 1 27 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 44 2 0.0 4 1 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 70 13 0.0 4 0 0 0 0 13 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 127 186 0.0 4 0 0 0 0 186 130 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 5 0.0 4 0 0 0 0 5 166 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 1 183 2 0.0 4 0 0 0 1 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.96 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 16,443 Reads with adapters: 1,329 (8.1%) Reads written (passing filters): 16,443 (100.0%) Total basepairs processed: 4,481,662 bp Total written (filtered): 4,449,984 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1329 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.8% C: 10.1% G: 76.9% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 72 256.9 0 72 4 10 64.2 0 10 9 9 0.1 0 0 9 10 14 0.0 1 1 13 11 1013 0.0 1 25 988 12 114 0.0 1 1 113 14 1 0.0 1 0 1 15 2 0.0 1 0 2 20 5 0.0 1 3 2 74 2 0.0 1 2 75 1 0.0 1 0 1 96 2 0.0 1 2 115 1 0.0 1 1 140 1 0.0 1 1 152 5 0.0 1 4 1 153 1 0.0 1 1 210 12 0.0 1 6 6 222 1 0.0 1 1 223 1 0.0 1 1 225 57 0.0 1 55 2 226 3 0.0 1 2 1 227 1 0.0 1 1 268 1 0.0 1 1