This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 133.54 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 150,395 (5.1%) Reads that were too short: 39,010 (1.3%) Reads written (passing filters): 111,385 (3.8%) Total basepairs processed: 887,907,860 bp Total written (filtered): 20,609,659 bp (2.3%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 150395 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 340 720.2 0 340 7 19667 720.2 0 19667 8 230 720.2 0 230 9 14912 720.2 0 14912 10 29 720.2 0 29 11 10 720.2 0 10 12 13 720.2 0 13 13 19 720.2 0 19 14 17 720.2 0 17 15 19 720.2 0 19 16 19 720.2 0 19 17 20 720.2 0 20 18 27 720.2 0 27 19 18 720.2 0 18 20 36 720.2 0 36 21 20 720.2 0 20 22 15 720.2 0 15 23 21 720.2 0 21 24 10 720.2 0 10 25 19 720.2 0 19 26 21 720.2 0 21 27 21 720.2 0 21 28 18 720.2 0 18 29 26 720.2 0 26 30 22 720.2 0 22 31 14 720.2 0 14 32 27 720.2 0 27 33 42 720.2 0 42 34 30 720.2 0 30 35 31 720.2 0 31 36 38 720.2 0 38 37 45 720.2 0 45 38 21 720.2 0 21 39 27 720.2 0 27 40 14 720.2 0 14 41 34 720.2 0 34 42 365 720.2 0 365 43 46 720.2 0 46 44 22 720.2 0 22 45 26 720.2 0 26 46 27 720.2 0 27 47 17 720.2 0 17 48 33 720.2 0 33 49 19 720.2 0 19 50 24 720.2 0 24 51 20 720.2 0 20 52 22 720.2 0 22 53 29 720.2 0 29 54 38 720.2 0 38 55 31 720.2 0 31 56 54 720.2 0 54 57 32 720.2 0 32 58 17 720.2 0 17 59 51 720.2 0 51 60 21 720.2 0 21 61 21 720.2 0 21 62 18 720.2 0 18 63 18 720.2 0 18 64 39 720.2 0 39 65 51 720.2 0 51 66 39 720.2 0 39 67 18 720.2 0 18 68 12 720.2 0 12 69 18 720.2 0 18 70 33 720.2 0 33 71 32 720.2 0 32 72 70 720.2 0 70 73 38 720.2 0 38 74 105 720.2 0 105 75 102 720.2 0 102 76 210 720.2 0 210 77 26 720.2 0 26 78 58 720.2 0 58 79 85 720.2 0 85 80 206 720.2 0 206 81 96 720.2 0 96 82 109 720.2 0 109 83 137 720.2 0 137 84 528 720.2 0 528 85 184 720.2 0 184 86 54 720.2 0 54 87 75 720.2 0 75 88 74 720.2 0 74 89 73 720.2 0 73 90 46 720.2 0 46 91 67 720.2 0 67 92 53 720.2 0 53 93 45 720.2 0 45 94 35 720.2 0 35 95 63 720.2 0 63 96 44 720.2 0 44 97 32 720.2 0 32 98 33 720.2 0 33 99 40 720.2 0 40 100 30 720.2 0 30 101 52 720.2 0 52 102 66 720.2 0 66 103 78 720.2 0 78 104 98 720.2 0 98 105 121 720.2 0 121 106 135 720.2 0 135 107 1755 720.2 0 1755 108 366 720.2 0 366 109 1838 720.2 0 1838 110 75 720.2 0 75 111 105 720.2 0 105 112 77 720.2 0 77 113 68 720.2 0 68 114 54 720.2 0 54 115 87 720.2 0 87 116 108 720.2 0 108 117 130 720.2 0 130 118 82 720.2 0 82 119 93 720.2 0 93 120 176 720.2 0 176 121 329 720.2 0 329 122 397 720.2 0 397 123 138 720.2 0 138 124 138 720.2 0 138 125 182 720.2 0 182 126 158 720.2 0 158 127 84 720.2 0 84 128 84 720.2 0 84 129 51 720.2 0 51 130 81 720.2 0 81 131 72 720.2 0 72 132 68 720.2 0 68 133 52 720.2 0 52 134 53 720.2 0 53 135 54 720.2 0 54 136 52 720.2 0 52 137 80 720.2 0 80 138 94 720.2 0 94 139 94 720.2 0 94 140 61 720.2 0 61 141 78 720.2 0 78 142 79 720.2 0 79 143 66 720.2 0 66 144 62 720.2 0 62 145 71 720.2 0 71 146 105 720.2 0 105 147 96 720.2 0 96 148 92 720.2 0 92 149 73 720.2 0 73 150 55 720.2 0 55 151 83 720.2 0 83 152 68 720.2 0 68 153 111 720.2 0 111 154 61 720.2 0 61 155 77 720.2 0 77 156 76 720.2 0 76 157 118 720.2 0 118 158 69 720.2 0 69 159 91 720.2 0 91 160 106 720.2 0 106 161 87 720.2 0 87 162 99 720.2 0 99 163 105 720.2 0 105 164 162 720.2 0 162 165 153 720.2 0 153 166 1235 720.2 0 1235 167 967 720.2 0 967 168 3327 720.2 0 3327 169 158 720.2 0 158 170 943 720.2 0 943 171 1473 720.2 0 1473 172 1040 720.2 0 1040 173 134 720.2 0 134 174 127 720.2 0 127 175 173 720.2 0 173 176 228 720.2 0 228 177 319 720.2 0 319 178 259 720.2 0 259 179 349 720.2 0 349 180 525 720.2 0 525 181 14142 720.2 0 14142 182 27304 720.2 0 27304 183 3530 720.2 0 3530 184 1166 720.2 0 1166 185 1082 720.2 0 1082 186 870 720.2 0 870 187 84 720.2 0 84 188 60 720.2 0 60 189 86 720.2 0 86 190 88 720.2 0 88 191 87 720.2 0 87 192 69 720.2 0 69 193 69 720.2 0 69 194 61 720.2 0 61 195 70 720.2 0 70 196 112 720.2 0 112 197 96 720.2 0 96 198 269 720.2 0 269 199 236 720.2 0 236 200 487 720.2 0 487 201 228 720.2 0 228 202 91 720.2 0 91 203 58 720.2 0 58 204 177 720.2 0 177 205 375 720.2 0 375 206 255 720.2 0 255 207 193 720.2 0 193 208 89 720.2 0 89 209 126 720.2 0 126 210 160 720.2 0 160 211 227 720.2 0 227 212 296 720.2 0 296 213 3880 720.2 0 3880 214 2472 720.2 0 2472 215 9750 720.2 0 9750 216 374 720.2 0 374 217 86 720.2 0 86 218 137 720.2 0 137 219 106 720.2 0 106 220 105 720.2 0 105 221 65 720.2 0 65 222 57 720.2 0 57 223 72 720.2 0 72 224 102 720.2 0 102 225 288 720.2 0 288 226 147 720.2 0 147 227 312 720.2 0 312 228 432 720.2 0 432 229 458 720.2 0 458 230 695 720.2 0 695 231 59 720.2 0 59 232 53 720.2 0 53 233 61 720.2 0 61 234 55 720.2 0 55 235 60 720.2 0 60 236 48 720.2 0 48 237 81 720.2 0 81 238 96 720.2 0 96 239 142 720.2 0 142 240 86 720.2 0 86 241 89 720.2 0 89 242 126 720.2 0 126 243 174 720.2 0 174 244 105 720.2 0 105 245 113 720.2 0 113 246 156 720.2 0 156 247 103 720.2 0 103 248 138 720.2 0 138 249 117 720.2 0 117 250 135 720.2 0 135 251 90 720.2 0 90 252 85 720.2 0 85 253 111 720.2 0 111 254 182 720.2 0 182 255 175 720.2 0 175 256 152 720.2 0 152 257 129 720.2 0 129 258 95 720.2 0 95 259 98 720.2 0 98 260 98 720.2 0 98 261 131 720.2 0 131 262 181 720.2 0 181 263 309 720.2 0 309 264 296 720.2 0 296 265 168 720.2 0 168 266 207 720.2 0 207 267 2751 720.2 0 2751 268 2897 720.2 0 2897 269 2989 720.2 0 2989 270 302 720.2 0 302 271 61 720.2 0 61 272 54 720.2 0 54 273 78 720.2 0 78 274 73 720.2 0 73 275 121 720.2 0 121 276 126 720.2 0 126 277 102 720.2 0 102 278 86 720.2 0 86 279 96 720.2 0 96 280 472 720.2 0 472 281 608 720.2 0 608 282 457 720.2 0 457 283 114 720.2 0 114 284 57 720.2 0 57 285 61 720.2 0 61 286 67 720.2 0 67 287 82 720.2 0 82 288 90 720.2 0 90 289 70 720.2 0 70 290 77 720.2 0 77 291 73 720.2 0 73 292 107 720.2 0 107 293 91 720.2 0 91 294 65 720.2 0 65 295 78 720.2 0 78 296 87 720.2 0 87 297 88 720.2 0 88 298 86 720.2 0 86 299 98 720.2 0 98 300 108 720.2 0 108 301 149 720.2 0 149 Finished in 6.37 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 111,385 Reads with adapters: 15,331 (13.8%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 15,299 (13.7%) Total basepairs processed: 20,609,659 bp Total written (filtered): 4,193,082 bp (20.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15331 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 105 0.0 3 0 100 5 17 15021 0.0 3 14714 295 11 1 18 39 0.0 3 4 33 1 1 19 17 0.0 3 4 0 12 1 20 25 0.0 3 0 0 0 25 21 46 0.0 3 0 0 0 46 22 2 0.0 3 0 0 0 2 24 15 0.0 3 0 0 0 15 25 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 1 1 62 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 1 2 71 6 0.0 3 0 0 0 6 72 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 109 1 0.0 3 0 1 122 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 167 1 0.0 3 1 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 15,299 Reads with adapters: 447 (2.9%) Reads written (passing filters): 15,299 (100.0%) Total basepairs processed: 4,193,082 bp Total written (filtered): 4,191,709 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 447 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.2% C: 5.4% G: 16.1% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 415 239.0 0 415 4 32 59.8 0 32