This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 131.93 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 60,551 (2.1%) Reads that were too short: 31,912 (1.1%) Reads written (passing filters): 28,639 (1.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 4,838,048 bp (0.5%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 60551 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 30 720.2 0 30 7 67 720.2 0 67 8 439 720.2 0 439 9 379 720.2 0 379 10 35 720.2 0 35 11 31 720.2 0 31 12 40 720.2 0 40 13 40 720.2 0 40 14 47 720.2 0 47 15 23 720.2 0 23 16 25 720.2 0 25 17 31 720.2 0 31 18 33 720.2 0 33 19 22 720.2 0 22 20 39 720.2 0 39 21 36 720.2 0 36 22 30 720.2 0 30 23 32 720.2 0 32 24 37 720.2 0 37 25 38 720.2 0 38 26 22 720.2 0 22 27 28 720.2 0 28 28 27 720.2 0 27 29 34 720.2 0 34 30 27 720.2 0 27 31 27 720.2 0 27 32 23 720.2 0 23 33 29 720.2 0 29 34 34 720.2 0 34 35 39 720.2 0 39 36 48 720.2 0 48 37 43 720.2 0 43 38 40 720.2 0 40 39 39 720.2 0 39 40 48 720.2 0 48 41 57 720.2 0 57 42 43 720.2 0 43 43 24 720.2 0 24 44 33 720.2 0 33 45 40 720.2 0 40 46 50 720.2 0 50 47 24 720.2 0 24 48 39 720.2 0 39 49 43 720.2 0 43 50 55 720.2 0 55 51 45 720.2 0 45 52 41 720.2 0 41 53 75 720.2 0 75 54 55 720.2 0 55 55 128 720.2 0 128 56 61 720.2 0 61 57 36 720.2 0 36 58 54 720.2 0 54 59 65 720.2 0 65 60 31 720.2 0 31 61 60 720.2 0 60 62 39 720.2 0 39 63 118 720.2 0 118 64 100 720.2 0 100 65 130 720.2 0 130 66 38 720.2 0 38 67 55 720.2 0 55 68 76 720.2 0 76 69 115 720.2 0 115 70 57 720.2 0 57 71 88 720.2 0 88 72 475 720.2 0 475 73 1332 720.2 0 1332 74 1060 720.2 0 1060 75 1144 720.2 0 1144 76 32 720.2 0 32 77 37 720.2 0 37 78 40 720.2 0 40 79 51 720.2 0 51 80 51 720.2 0 51 81 19 720.2 0 19 82 45 720.2 0 45 83 37 720.2 0 37 84 25 720.2 0 25 85 56 720.2 0 56 86 71 720.2 0 71 87 79 720.2 0 79 88 201 720.2 0 201 89 80 720.2 0 80 90 36 720.2 0 36 91 67 720.2 0 67 92 55 720.2 0 55 93 25 720.2 0 25 94 41 720.2 0 41 95 45 720.2 0 45 96 53 720.2 0 53 97 34 720.2 0 34 98 35 720.2 0 35 99 41 720.2 0 41 100 87 720.2 0 87 101 35 720.2 0 35 102 40 720.2 0 40 103 41 720.2 0 41 104 38 720.2 0 38 105 204 720.2 0 204 106 54 720.2 0 54 107 80 720.2 0 80 108 72 720.2 0 72 109 67 720.2 0 67 110 52 720.2 0 52 111 60 720.2 0 60 112 30 720.2 0 30 113 33 720.2 0 33 114 33 720.2 0 33 115 54 720.2 0 54 116 44 720.2 0 44 117 30 720.2 0 30 118 50 720.2 0 50 119 42 720.2 0 42 120 32 720.2 0 32 121 46 720.2 0 46 122 40 720.2 0 40 123 32 720.2 0 32 124 33 720.2 0 33 125 29 720.2 0 29 126 48 720.2 0 48 127 45 720.2 0 45 128 98 720.2 0 98 129 100 720.2 0 100 130 78 720.2 0 78 131 57 720.2 0 57 132 50 720.2 0 50 133 51 720.2 0 51 134 71 720.2 0 71 135 57 720.2 0 57 136 96 720.2 0 96 137 108 720.2 0 108 138 140 720.2 0 140 139 37 720.2 0 37 140 40 720.2 0 40 141 80 720.2 0 80 142 59 720.2 0 59 143 86 720.2 0 86 144 69 720.2 0 69 145 225 720.2 0 225 146 354 720.2 0 354 147 617 720.2 0 617 148 278 720.2 0 278 149 123 720.2 0 123 150 116 720.2 0 116 151 76 720.2 0 76 152 57 720.2 0 57 153 78 720.2 0 78 154 92 720.2 0 92 155 146 720.2 0 146 156 247 720.2 0 247 157 192 720.2 0 192 158 645 720.2 0 645 159 695 720.2 0 695 160 1298 720.2 0 1298 161 165 720.2 0 165 162 130 720.2 0 130 163 145 720.2 0 145 164 62 720.2 0 62 165 51 720.2 0 51 166 85 720.2 0 85 167 174 720.2 0 174 168 99 720.2 0 99 169 136 720.2 0 136 170 119 720.2 0 119 171 170 720.2 0 170 172 132 720.2 0 132 173 120 720.2 0 120 174 1377 720.2 0 1377 175 615 720.2 0 615 176 1905 720.2 0 1905 177 146 720.2 0 146 178 71 720.2 0 71 179 54 720.2 0 54 180 110 720.2 0 110 181 169 720.2 0 169 182 197 720.2 0 197 183 102 720.2 0 102 184 107 720.2 0 107 185 239 720.2 0 239 186 313 720.2 0 313 187 1579 720.2 0 1579 188 994 720.2 0 994 189 1307 720.2 0 1307 190 71 720.2 0 71 191 46 720.2 0 46 192 58 720.2 0 58 193 52 720.2 0 52 194 58 720.2 0 58 195 65 720.2 0 65 196 67 720.2 0 67 197 53 720.2 0 53 198 36 720.2 0 36 199 36 720.2 0 36 200 72 720.2 0 72 201 81 720.2 0 81 202 78 720.2 0 78 203 81 720.2 0 81 204 165 720.2 0 165 205 119 720.2 0 119 206 118 720.2 0 118 207 90 720.2 0 90 208 54 720.2 0 54 209 67 720.2 0 67 210 46 720.2 0 46 211 56 720.2 0 56 212 57 720.2 0 57 213 55 720.2 0 55 214 57 720.2 0 57 215 70 720.2 0 70 216 88 720.2 0 88 217 81 720.2 0 81 218 95 720.2 0 95 219 73 720.2 0 73 220 77 720.2 0 77 221 70 720.2 0 70 222 55 720.2 0 55 223 81 720.2 0 81 224 75 720.2 0 75 225 85 720.2 0 85 226 69 720.2 0 69 227 134 720.2 0 134 228 100 720.2 0 100 229 213 720.2 0 213 230 76 720.2 0 76 231 117 720.2 0 117 232 131 720.2 0 131 233 173 720.2 0 173 234 110 720.2 0 110 235 98 720.2 0 98 236 102 720.2 0 102 237 138 720.2 0 138 238 173 720.2 0 173 239 197 720.2 0 197 240 130 720.2 0 130 241 88 720.2 0 88 242 86 720.2 0 86 243 89 720.2 0 89 244 124 720.2 0 124 245 132 720.2 0 132 246 139 720.2 0 139 247 141 720.2 0 141 248 293 720.2 0 293 249 351 720.2 0 351 250 561 720.2 0 561 251 13556 720.2 0 13556 252 1808 720.2 0 1808 253 1662 720.2 0 1662 254 141 720.2 0 141 255 127 720.2 0 127 256 89 720.2 0 89 257 63 720.2 0 63 258 119 720.2 0 119 259 149 720.2 0 149 260 259 720.2 0 259 261 325 720.2 0 325 262 528 720.2 0 528 263 441 720.2 0 441 264 251 720.2 0 251 265 57 720.2 0 57 266 41 720.2 0 41 267 65 720.2 0 65 268 159 720.2 0 159 269 263 720.2 0 263 270 144 720.2 0 144 271 97 720.2 0 97 272 209 720.2 0 209 273 130 720.2 0 130 274 150 720.2 0 150 275 153 720.2 0 153 276 112 720.2 0 112 277 129 720.2 0 129 278 153 720.2 0 153 279 250 720.2 0 250 280 216 720.2 0 216 281 241 720.2 0 241 282 232 720.2 0 232 283 211 720.2 0 211 284 100 720.2 0 100 285 190 720.2 0 190 286 179 720.2 0 179 287 114 720.2 0 114 288 109 720.2 0 109 289 81 720.2 0 81 290 110 720.2 0 110 291 241 720.2 0 241 292 219 720.2 0 219 293 160 720.2 0 160 294 180 720.2 0 180 295 305 720.2 0 305 296 164 720.2 0 164 297 228 720.2 0 228 298 223 720.2 0 223 299 316 720.2 0 316 300 332 720.2 0 332 301 273 720.2 0 273 Finished in 1.64 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 28,639 Reads with adapters: 403 (1.4%) Reads that were too short: 28 (0.1%) Reads written (passing filters): 375 (1.3%) Total basepairs processed: 4,838,048 bp Total written (filtered): 101,378 bp (2.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 403 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 5 0.0 3 0 4 0 0 1 20 359 0.0 4 338 13 5 2 1 21 1 0.0 4 1 22 4 0.0 4 1 1 0 1 1 23 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 55 3 0.0 4 0 0 0 0 3 56 2 0.0 4 0 0 0 1 1 73 1 0.0 4 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 155 10 0.0 4 0 0 0 0 10 157 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 375 Reads with adapters: 100 (26.7%) Reads written (passing filters): 375 (100.0%) Total basepairs processed: 101,378 bp Total written (filtered): 100,200 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 100 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 3.0% G: 91.0% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 1.5 0 1 9 43 0.0 0 3 40 10 41 0.0 1 0 41 11 14 0.0 1 0 14 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.