This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 133.26 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 41,189 (1.4%) Reads that were too short: 21,430 (0.7%) Reads written (passing filters): 19,759 (0.7%) Total basepairs processed: 887,907,860 bp Total written (filtered): 3,737,682 bp (0.4%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 41189 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 26 720.2 0 26 7 44 720.2 0 44 8 373 720.2 0 373 9 441 720.2 0 441 10 35 720.2 0 35 11 23 720.2 0 23 12 17 720.2 0 17 13 27 720.2 0 27 14 26 720.2 0 26 15 23 720.2 0 23 16 26 720.2 0 26 17 25 720.2 0 25 18 21 720.2 0 21 19 22 720.2 0 22 20 36 720.2 0 36 21 24 720.2 0 24 22 26 720.2 0 26 23 29 720.2 0 29 24 32 720.2 0 32 25 34 720.2 0 34 26 27 720.2 0 27 27 30 720.2 0 30 28 32 720.2 0 32 29 26 720.2 0 26 30 17 720.2 0 17 31 33 720.2 0 33 32 34 720.2 0 34 33 20 720.2 0 20 34 25 720.2 0 25 35 27 720.2 0 27 36 128 720.2 0 128 37 50 720.2 0 50 38 30 720.2 0 30 39 28 720.2 0 28 40 43 720.2 0 43 41 40 720.2 0 40 42 31 720.2 0 31 43 17 720.2 0 17 44 23 720.2 0 23 45 36 720.2 0 36 46 20 720.2 0 20 47 31 720.2 0 31 48 27 720.2 0 27 49 37 720.2 0 37 50 52 720.2 0 52 51 25 720.2 0 25 52 37 720.2 0 37 53 33 720.2 0 33 54 37 720.2 0 37 55 58 720.2 0 58 56 459 720.2 0 459 57 48 720.2 0 48 58 48 720.2 0 48 59 34 720.2 0 34 60 18 720.2 0 18 61 32 720.2 0 32 62 20 720.2 0 20 63 32 720.2 0 32 64 23 720.2 0 23 65 35 720.2 0 35 66 30 720.2 0 30 67 48 720.2 0 48 68 67 720.2 0 67 69 65 720.2 0 65 70 55 720.2 0 55 71 107 720.2 0 107 72 673 720.2 0 673 73 1857 720.2 0 1857 74 1371 720.2 0 1371 75 1565 720.2 0 1565 76 22 720.2 0 22 77 25 720.2 0 25 78 25 720.2 0 25 79 36 720.2 0 36 80 39 720.2 0 39 81 21 720.2 0 21 82 24 720.2 0 24 83 33 720.2 0 33 84 22 720.2 0 22 85 42 720.2 0 42 86 47 720.2 0 47 87 38 720.2 0 38 88 63 720.2 0 63 89 53 720.2 0 53 90 31 720.2 0 31 91 28 720.2 0 28 92 26 720.2 0 26 93 30 720.2 0 30 94 30 720.2 0 30 95 32 720.2 0 32 96 31 720.2 0 31 97 22 720.2 0 22 98 14 720.2 0 14 99 28 720.2 0 28 100 67 720.2 0 67 101 39 720.2 0 39 102 26 720.2 0 26 103 30 720.2 0 30 104 34 720.2 0 34 105 179 720.2 0 179 106 36 720.2 0 36 107 68 720.2 0 68 108 37 720.2 0 37 109 50 720.2 0 50 110 35 720.2 0 35 111 39 720.2 0 39 112 25 720.2 0 25 113 27 720.2 0 27 114 27 720.2 0 27 115 45 720.2 0 45 116 28 720.2 0 28 117 31 720.2 0 31 118 28 720.2 0 28 119 25 720.2 0 25 120 26 720.2 0 26 121 36 720.2 0 36 122 23 720.2 0 23 123 27 720.2 0 27 124 26 720.2 0 26 125 27 720.2 0 27 126 31 720.2 0 31 127 34 720.2 0 34 128 60 720.2 0 60 129 59 720.2 0 59 130 37 720.2 0 37 131 30 720.2 0 30 132 25 720.2 0 25 133 20 720.2 0 20 134 54 720.2 0 54 135 27 720.2 0 27 136 67 720.2 0 67 137 56 720.2 0 56 138 155 720.2 0 155 139 22 720.2 0 22 140 28 720.2 0 28 141 42 720.2 0 42 142 33 720.2 0 33 143 42 720.2 0 42 144 39 720.2 0 39 145 207 720.2 0 207 146 313 720.2 0 313 147 635 720.2 0 635 148 139 720.2 0 139 149 54 720.2 0 54 150 87 720.2 0 87 151 37 720.2 0 37 152 30 720.2 0 30 153 35 720.2 0 35 154 52 720.2 0 52 155 51 720.2 0 51 156 91 720.2 0 91 157 63 720.2 0 63 158 345 720.2 0 345 159 410 720.2 0 410 160 666 720.2 0 666 161 85 720.2 0 85 162 55 720.2 0 55 163 127 720.2 0 127 164 22 720.2 0 22 165 27 720.2 0 27 166 41 720.2 0 41 167 37 720.2 0 37 168 40 720.2 0 40 169 65 720.2 0 65 170 65 720.2 0 65 171 103 720.2 0 103 172 68 720.2 0 68 173 52 720.2 0 52 174 536 720.2 0 536 175 238 720.2 0 238 176 854 720.2 0 854 177 49 720.2 0 49 178 40 720.2 0 40 179 23 720.2 0 23 180 43 720.2 0 43 181 71 720.2 0 71 182 81 720.2 0 81 183 41 720.2 0 41 184 56 720.2 0 56 185 64 720.2 0 64 186 119 720.2 0 119 187 541 720.2 0 541 188 336 720.2 0 336 189 596 720.2 0 596 190 44 720.2 0 44 191 45 720.2 0 45 192 45 720.2 0 45 193 33 720.2 0 33 194 25 720.2 0 25 195 34 720.2 0 34 196 26 720.2 0 26 197 42 720.2 0 42 198 32 720.2 0 32 199 48 720.2 0 48 200 37 720.2 0 37 201 43 720.2 0 43 202 37 720.2 0 37 203 41 720.2 0 41 204 78 720.2 0 78 205 54 720.2 0 54 206 47 720.2 0 47 207 48 720.2 0 48 208 27 720.2 0 27 209 19 720.2 0 19 210 27 720.2 0 27 211 23 720.2 0 23 212 22 720.2 0 22 213 36 720.2 0 36 214 47 720.2 0 47 215 40 720.2 0 40 216 37 720.2 0 37 217 41 720.2 0 41 218 68 720.2 0 68 219 37 720.2 0 37 220 41 720.2 0 41 221 31 720.2 0 31 222 30 720.2 0 30 223 47 720.2 0 47 224 47 720.2 0 47 225 41 720.2 0 41 226 29 720.2 0 29 227 77 720.2 0 77 228 50 720.2 0 50 229 113 720.2 0 113 230 50 720.2 0 50 231 82 720.2 0 82 232 71 720.2 0 71 233 88 720.2 0 88 234 80 720.2 0 80 235 41 720.2 0 41 236 53 720.2 0 53 237 101 720.2 0 101 238 103 720.2 0 103 239 96 720.2 0 96 240 56 720.2 0 56 241 50 720.2 0 50 242 45 720.2 0 45 243 45 720.2 0 45 244 60 720.2 0 60 245 69 720.2 0 69 246 63 720.2 0 63 247 82 720.2 0 82 248 153 720.2 0 153 249 214 720.2 0 214 250 297 720.2 0 297 251 11225 720.2 0 11225 252 985 720.2 0 985 253 1101 720.2 0 1101 254 98 720.2 0 98 255 71 720.2 0 71 256 49 720.2 0 49 257 37 720.2 0 37 258 41 720.2 0 41 259 52 720.2 0 52 260 146 720.2 0 146 261 134 720.2 0 134 262 230 720.2 0 230 263 157 720.2 0 157 264 97 720.2 0 97 265 39 720.2 0 39 266 42 720.2 0 42 267 47 720.2 0 47 268 83 720.2 0 83 269 116 720.2 0 116 270 59 720.2 0 59 271 62 720.2 0 62 272 105 720.2 0 105 273 178 720.2 0 178 274 98 720.2 0 98 275 111 720.2 0 111 276 76 720.2 0 76 277 58 720.2 0 58 278 88 720.2 0 88 279 112 720.2 0 112 280 120 720.2 0 120 281 91 720.2 0 91 282 102 720.2 0 102 283 61 720.2 0 61 284 61 720.2 0 61 285 212 720.2 0 212 286 64 720.2 0 64 287 57 720.2 0 57 288 79 720.2 0 79 289 58 720.2 0 58 290 65 720.2 0 65 291 97 720.2 0 97 292 104 720.2 0 104 293 116 720.2 0 116 294 111 720.2 0 111 295 253 720.2 0 253 296 83 720.2 0 83 297 147 720.2 0 147 298 120 720.2 0 120 299 175 720.2 0 175 300 278 720.2 0 278 301 145 720.2 0 145 Finished in 1.09 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 19,759 Reads with adapters: 381 (1.9%) Reads that were too short: 18 (0.1%) Reads written (passing filters): 363 (1.8%) Total basepairs processed: 3,737,682 bp Total written (filtered): 99,233 bp (2.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 381 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 2 0.0 3 0 1 0 1 17 351 0.0 3 345 4 2 19 1 0.0 3 1 20 1 0.0 3 1 26 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 196 1 0.0 3 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (83 us/read; 0.73 M reads/minute). === Summary === Total reads processed: 363 Reads with adapters: 1 (0.3%) Reads written (passing filters): 363 (100.0%) Total basepairs processed: 99,233 bp Total written (filtered): 99,230 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 5.7 0 1