This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 135.04 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 55,087 (1.9%) Reads that were too short: 17,695 (0.6%) Reads written (passing filters): 37,392 (1.3%) Total basepairs processed: 887,907,860 bp Total written (filtered): 6,238,681 bp (0.7%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 55087 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 720.2 0 129 7 3166 720.2 0 3166 8 2686 720.2 0 2686 9 17 720.2 0 17 10 31 720.2 0 31 11 59 720.2 0 59 12 38 720.2 0 38 13 167 720.2 0 167 14 100 720.2 0 100 15 278 720.2 0 278 16 44 720.2 0 44 17 13 720.2 0 13 18 14 720.2 0 14 19 13 720.2 0 13 20 17 720.2 0 17 21 22 720.2 0 22 22 10 720.2 0 10 23 21 720.2 0 21 24 34 720.2 0 34 25 75 720.2 0 75 26 79 720.2 0 79 27 184 720.2 0 184 28 18 720.2 0 18 29 12 720.2 0 12 30 20 720.2 0 20 31 15 720.2 0 15 32 20 720.2 0 20 33 13 720.2 0 13 34 7 720.2 0 7 35 20 720.2 0 20 36 15 720.2 0 15 37 17 720.2 0 17 38 18 720.2 0 18 39 24 720.2 0 24 40 10 720.2 0 10 41 14 720.2 0 14 42 19 720.2 0 19 43 30 720.2 0 30 44 12 720.2 0 12 45 16 720.2 0 16 46 10 720.2 0 10 47 21 720.2 0 21 48 24 720.2 0 24 49 22 720.2 0 22 50 31 720.2 0 31 51 15 720.2 0 15 52 5 720.2 0 5 53 16 720.2 0 16 54 34 720.2 0 34 55 24 720.2 0 24 56 32 720.2 0 32 57 30 720.2 0 30 58 28 720.2 0 28 59 138 720.2 0 138 60 46 720.2 0 46 61 77 720.2 0 77 62 41 720.2 0 41 63 21 720.2 0 21 64 24 720.2 0 24 65 21 720.2 0 21 66 24 720.2 0 24 67 10 720.2 0 10 68 23 720.2 0 23 69 17 720.2 0 17 70 15 720.2 0 15 71 27 720.2 0 27 72 16 720.2 0 16 73 21 720.2 0 21 74 22 720.2 0 22 75 29 720.2 0 29 76 21 720.2 0 21 77 30 720.2 0 30 78 34 720.2 0 34 79 38 720.2 0 38 80 90 720.2 0 90 81 58 720.2 0 58 82 697 720.2 0 697 83 266 720.2 0 266 84 461 720.2 0 461 85 7 720.2 0 7 86 27 720.2 0 27 87 25 720.2 0 25 88 13 720.2 0 13 89 26 720.2 0 26 90 21 720.2 0 21 91 21 720.2 0 21 92 13 720.2 0 13 93 32 720.2 0 32 94 23 720.2 0 23 95 24 720.2 0 24 96 34 720.2 0 34 97 33 720.2 0 33 98 19 720.2 0 19 99 27 720.2 0 27 100 19 720.2 0 19 101 30 720.2 0 30 102 75 720.2 0 75 103 96 720.2 0 96 104 60 720.2 0 60 105 54 720.2 0 54 106 79 720.2 0 79 107 189 720.2 0 189 108 81 720.2 0 81 109 29 720.2 0 29 110 168 720.2 0 168 111 63 720.2 0 63 112 104 720.2 0 104 113 50 720.2 0 50 114 33 720.2 0 33 115 30 720.2 0 30 116 26 720.2 0 26 117 21 720.2 0 21 118 32 720.2 0 32 119 39 720.2 0 39 120 70 720.2 0 70 121 36 720.2 0 36 122 174 720.2 0 174 123 288 720.2 0 288 124 183 720.2 0 183 125 25 720.2 0 25 126 28 720.2 0 28 127 41 720.2 0 41 128 46 720.2 0 46 129 38 720.2 0 38 130 26 720.2 0 26 131 41 720.2 0 41 132 38 720.2 0 38 133 49 720.2 0 49 134 53 720.2 0 53 135 49 720.2 0 49 136 69 720.2 0 69 137 142 720.2 0 142 138 253 720.2 0 253 139 161 720.2 0 161 140 105 720.2 0 105 141 79 720.2 0 79 142 102 720.2 0 102 143 33 720.2 0 33 144 33 720.2 0 33 145 19 720.2 0 19 146 53 720.2 0 53 147 109 720.2 0 109 148 49 720.2 0 49 149 36 720.2 0 36 150 50 720.2 0 50 151 39 720.2 0 39 152 40 720.2 0 40 153 46 720.2 0 46 154 47 720.2 0 47 155 57 720.2 0 57 156 76 720.2 0 76 157 85 720.2 0 85 158 120 720.2 0 120 159 141 720.2 0 141 160 500 720.2 0 500 161 724 720.2 0 724 162 979 720.2 0 979 163 263 720.2 0 263 164 96 720.2 0 96 165 78 720.2 0 78 166 31 720.2 0 31 167 64 720.2 0 64 168 62 720.2 0 62 169 62 720.2 0 62 170 110 720.2 0 110 171 31 720.2 0 31 172 58 720.2 0 58 173 80 720.2 0 80 174 97 720.2 0 97 175 124 720.2 0 124 176 119 720.2 0 119 177 124 720.2 0 124 178 88 720.2 0 88 179 95 720.2 0 95 180 389 720.2 0 389 181 893 720.2 0 893 182 1215 720.2 0 1215 183 667 720.2 0 667 184 162 720.2 0 162 185 235 720.2 0 235 186 337 720.2 0 337 187 4884 720.2 0 4884 188 2593 720.2 0 2593 189 6900 720.2 0 6900 190 50 720.2 0 50 191 43 720.2 0 43 192 32 720.2 0 32 193 38 720.2 0 38 194 44 720.2 0 44 195 25 720.2 0 25 196 34 720.2 0 34 197 62 720.2 0 62 198 42 720.2 0 42 199 52 720.2 0 52 200 60 720.2 0 60 201 42 720.2 0 42 202 85 720.2 0 85 203 135 720.2 0 135 204 187 720.2 0 187 205 156 720.2 0 156 206 253 720.2 0 253 207 167 720.2 0 167 208 85 720.2 0 85 209 49 720.2 0 49 210 176 720.2 0 176 211 357 720.2 0 357 212 611 720.2 0 611 213 249 720.2 0 249 214 153 720.2 0 153 215 101 720.2 0 101 216 48 720.2 0 48 217 79 720.2 0 79 218 51 720.2 0 51 219 153 720.2 0 153 220 226 720.2 0 226 221 168 720.2 0 168 222 130 720.2 0 130 223 66 720.2 0 66 224 78 720.2 0 78 225 176 720.2 0 176 226 189 720.2 0 189 227 173 720.2 0 173 228 107 720.2 0 107 229 168 720.2 0 168 230 1036 720.2 0 1036 231 1649 720.2 0 1649 232 1195 720.2 0 1195 233 121 720.2 0 121 234 40 720.2 0 40 235 29 720.2 0 29 236 27 720.2 0 27 237 23 720.2 0 23 238 33 720.2 0 33 239 43 720.2 0 43 240 54 720.2 0 54 241 73 720.2 0 73 242 43 720.2 0 43 243 31 720.2 0 31 244 40 720.2 0 40 245 42 720.2 0 42 246 31 720.2 0 31 247 26 720.2 0 26 248 21 720.2 0 21 249 29 720.2 0 29 250 27 720.2 0 27 251 28 720.2 0 28 252 33 720.2 0 33 253 60 720.2 0 60 254 87 720.2 0 87 255 58 720.2 0 58 256 64 720.2 0 64 257 57 720.2 0 57 258 62 720.2 0 62 259 52 720.2 0 52 260 63 720.2 0 63 261 50 720.2 0 50 262 54 720.2 0 54 263 65 720.2 0 65 264 67 720.2 0 67 265 55 720.2 0 55 266 54 720.2 0 54 267 57 720.2 0 57 268 94 720.2 0 94 269 78 720.2 0 78 270 47 720.2 0 47 271 60 720.2 0 60 272 62 720.2 0 62 273 124 720.2 0 124 274 160 720.2 0 160 275 475 720.2 0 475 276 401 720.2 0 401 277 672 720.2 0 672 278 250 720.2 0 250 279 311 720.2 0 311 280 301 720.2 0 301 281 210 720.2 0 210 282 84 720.2 0 84 283 134 720.2 0 134 284 165 720.2 0 165 285 195 720.2 0 195 286 100 720.2 0 100 287 108 720.2 0 108 288 225 720.2 0 225 289 683 720.2 0 683 290 926 720.2 0 926 291 218 720.2 0 218 292 59 720.2 0 59 293 67 720.2 0 67 294 124 720.2 0 124 295 115 720.2 0 115 296 90 720.2 0 90 297 72 720.2 0 72 298 129 720.2 0 129 299 86 720.2 0 86 300 273 720.2 0 273 301 442 720.2 0 442 Finished in 2.18 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 37,392 Reads with adapters: 2,855 (7.6%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 2,844 (7.6%) Total basepairs processed: 6,238,681 bp Total written (filtered): 770,256 bp (12.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2855 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 2 1 19 28 0.0 3 0 26 1 1 20 2724 0.0 4 2578 123 13 8 2 21 17 0.0 4 6 7 2 1 1 22 5 0.0 4 4 0 1 23 10 0.0 4 1 0 0 7 2 39 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 57 0.0 4 0 0 0 0 57 128 1 0.0 4 0 0 1 175 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 2,844 Reads with adapters: 555 (19.5%) Reads written (passing filters): 2,844 (100.0%) Total basepairs processed: 770,256 bp Total written (filtered): 762,323 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 555 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 5.4% G: 88.5% T: 3.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 44.4 0 5 5 1 2.8 0 1 9 16 0.0 0 0 16 10 140 0.0 1 6 134 11 375 0.0 1 9 366 12 7 0.0 1 0 7 22 1 0.0 1 1 151 1 0.0 1 1 209 2 0.0 1 0 2 224 6 0.0 1 5 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.