This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 132.02 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 44,174 (1.5%) Reads that were too short: 10,096 (0.3%) Reads written (passing filters): 34,078 (1.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 5,732,352 bp (0.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 44174 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 87 720.2 0 87 7 2873 720.2 0 2873 8 3247 720.2 0 3247 9 12 720.2 0 12 10 8 720.2 0 8 11 12 720.2 0 12 12 15 720.2 0 15 13 92 720.2 0 92 14 82 720.2 0 82 15 146 720.2 0 146 16 44 720.2 0 44 17 6 720.2 0 6 18 7 720.2 0 7 19 4 720.2 0 4 20 7 720.2 0 7 21 10 720.2 0 10 22 8 720.2 0 8 23 17 720.2 0 17 24 7 720.2 0 7 25 183 720.2 0 183 26 89 720.2 0 89 27 7 720.2 0 7 28 6 720.2 0 6 29 6 720.2 0 6 30 14 720.2 0 14 31 11 720.2 0 11 32 10 720.2 0 10 33 12 720.2 0 12 34 6 720.2 0 6 35 9 720.2 0 9 36 8 720.2 0 8 37 5 720.2 0 5 38 11 720.2 0 11 39 4 720.2 0 4 40 14 720.2 0 14 41 19 720.2 0 19 42 4 720.2 0 4 43 7 720.2 0 7 44 6 720.2 0 6 45 6 720.2 0 6 46 10 720.2 0 10 47 17 720.2 0 17 48 25 720.2 0 25 49 17 720.2 0 17 50 10 720.2 0 10 51 10 720.2 0 10 52 6 720.2 0 6 53 19 720.2 0 19 54 13 720.2 0 13 55 18 720.2 0 18 56 25 720.2 0 25 57 23 720.2 0 23 58 19 720.2 0 19 59 103 720.2 0 103 60 35 720.2 0 35 61 52 720.2 0 52 62 22 720.2 0 22 63 17 720.2 0 17 64 10 720.2 0 10 65 10 720.2 0 10 66 12 720.2 0 12 67 14 720.2 0 14 68 17 720.2 0 17 69 13 720.2 0 13 70 19 720.2 0 19 71 28 720.2 0 28 72 19 720.2 0 19 73 15 720.2 0 15 74 12 720.2 0 12 75 13 720.2 0 13 76 22 720.2 0 22 77 19 720.2 0 19 78 27 720.2 0 27 79 19 720.2 0 19 80 53 720.2 0 53 81 70 720.2 0 70 82 1219 720.2 0 1219 83 420 720.2 0 420 84 812 720.2 0 812 85 11 720.2 0 11 86 24 720.2 0 24 87 19 720.2 0 19 88 15 720.2 0 15 89 24 720.2 0 24 90 23 720.2 0 23 91 29 720.2 0 29 92 25 720.2 0 25 93 20 720.2 0 20 94 32 720.2 0 32 95 41 720.2 0 41 96 32 720.2 0 32 97 24 720.2 0 24 98 27 720.2 0 27 99 13 720.2 0 13 100 18 720.2 0 18 101 21 720.2 0 21 102 39 720.2 0 39 103 38 720.2 0 38 104 45 720.2 0 45 105 43 720.2 0 43 106 72 720.2 0 72 107 179 720.2 0 179 108 90 720.2 0 90 109 22 720.2 0 22 110 170 720.2 0 170 111 41 720.2 0 41 112 89 720.2 0 89 113 49 720.2 0 49 114 25 720.2 0 25 115 28 720.2 0 28 116 17 720.2 0 17 117 24 720.2 0 24 118 32 720.2 0 32 119 38 720.2 0 38 120 39 720.2 0 39 121 18 720.2 0 18 122 137 720.2 0 137 123 137 720.2 0 137 124 109 720.2 0 109 125 25 720.2 0 25 126 21 720.2 0 21 127 25 720.2 0 25 128 31 720.2 0 31 129 32 720.2 0 32 130 22 720.2 0 22 131 40 720.2 0 40 132 25 720.2 0 25 133 45 720.2 0 45 134 40 720.2 0 40 135 27 720.2 0 27 136 24 720.2 0 24 137 66 720.2 0 66 138 79 720.2 0 79 139 69 720.2 0 69 140 66 720.2 0 66 141 48 720.2 0 48 142 53 720.2 0 53 143 29 720.2 0 29 144 26 720.2 0 26 145 24 720.2 0 24 146 40 720.2 0 40 147 115 720.2 0 115 148 42 720.2 0 42 149 19 720.2 0 19 150 26 720.2 0 26 151 30 720.2 0 30 152 38 720.2 0 38 153 29 720.2 0 29 154 41 720.2 0 41 155 36 720.2 0 36 156 31 720.2 0 31 157 45 720.2 0 45 158 55 720.2 0 55 159 66 720.2 0 66 160 229 720.2 0 229 161 191 720.2 0 191 162 334 720.2 0 334 163 57 720.2 0 57 164 49 720.2 0 49 165 30 720.2 0 30 166 29 720.2 0 29 167 23 720.2 0 23 168 42 720.2 0 42 169 49 720.2 0 49 170 88 720.2 0 88 171 30 720.2 0 30 172 38 720.2 0 38 173 56 720.2 0 56 174 61 720.2 0 61 175 85 720.2 0 85 176 51 720.2 0 51 177 55 720.2 0 55 178 36 720.2 0 36 179 39 720.2 0 39 180 148 720.2 0 148 181 276 720.2 0 276 182 357 720.2 0 357 183 201 720.2 0 201 184 141 720.2 0 141 185 223 720.2 0 223 186 317 720.2 0 317 187 5756 720.2 0 5756 188 2837 720.2 0 2837 189 8133 720.2 0 8133 190 59 720.2 0 59 191 28 720.2 0 28 192 44 720.2 0 44 193 20 720.2 0 20 194 30 720.2 0 30 195 26 720.2 0 26 196 24 720.2 0 24 197 28 720.2 0 28 198 34 720.2 0 34 199 36 720.2 0 36 200 33 720.2 0 33 201 41 720.2 0 41 202 26 720.2 0 26 203 61 720.2 0 61 204 62 720.2 0 62 205 69 720.2 0 69 206 100 720.2 0 100 207 82 720.2 0 82 208 42 720.2 0 42 209 30 720.2 0 30 210 69 720.2 0 69 211 133 720.2 0 133 212 211 720.2 0 211 213 98 720.2 0 98 214 73 720.2 0 73 215 43 720.2 0 43 216 40 720.2 0 40 217 44 720.2 0 44 218 35 720.2 0 35 219 70 720.2 0 70 220 117 720.2 0 117 221 123 720.2 0 123 222 50 720.2 0 50 223 44 720.2 0 44 224 55 720.2 0 55 225 95 720.2 0 95 226 123 720.2 0 123 227 71 720.2 0 71 228 64 720.2 0 64 229 69 720.2 0 69 230 446 720.2 0 446 231 655 720.2 0 655 232 467 720.2 0 467 233 75 720.2 0 75 234 31 720.2 0 31 235 26 720.2 0 26 236 31 720.2 0 31 237 37 720.2 0 37 238 24 720.2 0 24 239 43 720.2 0 43 240 49 720.2 0 49 241 45 720.2 0 45 242 36 720.2 0 36 243 44 720.2 0 44 244 39 720.2 0 39 245 30 720.2 0 30 246 27 720.2 0 27 247 14 720.2 0 14 248 26 720.2 0 26 249 26 720.2 0 26 250 31 720.2 0 31 251 39 720.2 0 39 252 29 720.2 0 29 253 34 720.2 0 34 254 66 720.2 0 66 255 47 720.2 0 47 256 34 720.2 0 34 257 52 720.2 0 52 258 50 720.2 0 50 259 33 720.2 0 33 260 55 720.2 0 55 261 50 720.2 0 50 262 92 720.2 0 92 263 46 720.2 0 46 264 52 720.2 0 52 265 39 720.2 0 39 266 34 720.2 0 34 267 49 720.2 0 49 268 52 720.2 0 52 269 53 720.2 0 53 270 35 720.2 0 35 271 42 720.2 0 42 272 35 720.2 0 35 273 63 720.2 0 63 274 108 720.2 0 108 275 442 720.2 0 442 276 298 720.2 0 298 277 727 720.2 0 727 278 188 720.2 0 188 279 152 720.2 0 152 280 182 720.2 0 182 281 119 720.2 0 119 282 54 720.2 0 54 283 62 720.2 0 62 284 57 720.2 0 57 285 95 720.2 0 95 286 41 720.2 0 41 287 66 720.2 0 66 288 128 720.2 0 128 289 408 720.2 0 408 290 583 720.2 0 583 291 130 720.2 0 130 292 30 720.2 0 30 293 75 720.2 0 75 294 69 720.2 0 69 295 69 720.2 0 69 296 80 720.2 0 80 297 70 720.2 0 70 298 56 720.2 0 56 299 85 720.2 0 85 300 192 720.2 0 192 301 148 720.2 0 148 Finished in 1.82 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 34,078 Reads with adapters: 3,039 (8.9%) Reads that were too short: 68 (0.2%) Reads written (passing filters): 2,971 (8.7%) Total basepairs processed: 5,732,352 bp Total written (filtered): 799,430 bp (13.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3039 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 30 0.0 3 0 29 1 17 2784 0.0 3 2740 41 3 18 7 0.0 3 3 4 19 8 0.0 3 0 0 7 1 20 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 36 5 0.0 3 0 0 0 5 39 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 69 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 141 136 0.0 3 0 0 1 135 160 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 208 1 0.0 3 1 216 1 0.0 3 0 0 0 1 217 25 0.0 3 0 0 0 25 265 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 2,971 Reads with adapters: 5 (0.2%) Reads written (passing filters): 2,971 (100.0%) Total basepairs processed: 799,430 bp Total written (filtered): 799,414 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 40.0% G: 20.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 46.4 0 4 4 1 11.6 0 1