This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 128.66 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 58,072 (2.0%) Reads that were too short: 16,184 (0.5%) Reads written (passing filters): 41,888 (1.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 6,745,217 bp (0.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 58072 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 136 720.2 0 136 7 153 720.2 0 153 8 16 720.2 0 16 9 104 720.2 0 104 10 83 720.2 0 83 11 15 720.2 0 15 12 21 720.2 0 21 13 25 720.2 0 25 14 14 720.2 0 14 15 19 720.2 0 19 16 16 720.2 0 16 17 20 720.2 0 20 18 17 720.2 0 17 19 8 720.2 0 8 20 29 720.2 0 29 21 21 720.2 0 21 22 18 720.2 0 18 23 17 720.2 0 17 24 17 720.2 0 17 25 17 720.2 0 17 26 18 720.2 0 18 27 17 720.2 0 17 28 25 720.2 0 25 29 18 720.2 0 18 30 18 720.2 0 18 31 19 720.2 0 19 32 13 720.2 0 13 33 18 720.2 0 18 34 23 720.2 0 23 35 24 720.2 0 24 36 25 720.2 0 25 37 31 720.2 0 31 38 78 720.2 0 78 39 37 720.2 0 37 40 135 720.2 0 135 41 27 720.2 0 27 42 28 720.2 0 28 43 18 720.2 0 18 44 13 720.2 0 13 45 20 720.2 0 20 46 23 720.2 0 23 47 22 720.2 0 22 48 16 720.2 0 16 49 27 720.2 0 27 50 31 720.2 0 31 51 23 720.2 0 23 52 16 720.2 0 16 53 126 720.2 0 126 54 80 720.2 0 80 55 143 720.2 0 143 56 116 720.2 0 116 57 3120 720.2 0 3120 58 39 720.2 0 39 59 27 720.2 0 27 60 28 720.2 0 28 61 19 720.2 0 19 62 17 720.2 0 17 63 32 720.2 0 32 64 18 720.2 0 18 65 25 720.2 0 25 66 59 720.2 0 59 67 25 720.2 0 25 68 45 720.2 0 45 69 116 720.2 0 116 70 556 720.2 0 556 71 80 720.2 0 80 72 33 720.2 0 33 73 48 720.2 0 48 74 14 720.2 0 14 75 10 720.2 0 10 76 18 720.2 0 18 77 15 720.2 0 15 78 18 720.2 0 18 79 25 720.2 0 25 80 29 720.2 0 29 81 27 720.2 0 27 82 23 720.2 0 23 83 32 720.2 0 32 84 19 720.2 0 19 85 23 720.2 0 23 86 21 720.2 0 21 87 26 720.2 0 26 88 22 720.2 0 22 89 36 720.2 0 36 90 18 720.2 0 18 91 38 720.2 0 38 92 21 720.2 0 21 93 30 720.2 0 30 94 42 720.2 0 42 95 46 720.2 0 46 96 30 720.2 0 30 97 13 720.2 0 13 98 23 720.2 0 23 99 19 720.2 0 19 100 22 720.2 0 22 101 28 720.2 0 28 102 43 720.2 0 43 103 48 720.2 0 48 104 41 720.2 0 41 105 77 720.2 0 77 106 33 720.2 0 33 107 44 720.2 0 44 108 30 720.2 0 30 109 45 720.2 0 45 110 35 720.2 0 35 111 48 720.2 0 48 112 54 720.2 0 54 113 64 720.2 0 64 114 43 720.2 0 43 115 32 720.2 0 32 116 48 720.2 0 48 117 36 720.2 0 36 118 25 720.2 0 25 119 34 720.2 0 34 120 48 720.2 0 48 121 62 720.2 0 62 122 51 720.2 0 51 123 94 720.2 0 94 124 111 720.2 0 111 125 1375 720.2 0 1375 126 566 720.2 0 566 127 1796 720.2 0 1796 128 43 720.2 0 43 129 42 720.2 0 42 130 79 720.2 0 79 131 40 720.2 0 40 132 38 720.2 0 38 133 55 720.2 0 55 134 48 720.2 0 48 135 47 720.2 0 47 136 26 720.2 0 26 137 28 720.2 0 28 138 111 720.2 0 111 139 32 720.2 0 32 140 47 720.2 0 47 141 40 720.2 0 40 142 41 720.2 0 41 143 78 720.2 0 78 144 166 720.2 0 166 145 469 720.2 0 469 146 553 720.2 0 553 147 206 720.2 0 206 148 84 720.2 0 84 149 66 720.2 0 66 150 296 720.2 0 296 151 151 720.2 0 151 152 280 720.2 0 280 153 95 720.2 0 95 154 133 720.2 0 133 155 182 720.2 0 182 156 424 720.2 0 424 157 464 720.2 0 464 158 824 720.2 0 824 159 688 720.2 0 688 160 6480 720.2 0 6480 161 2674 720.2 0 2674 162 9844 720.2 0 9844 163 85 720.2 0 85 164 84 720.2 0 84 165 43 720.2 0 43 166 53 720.2 0 53 167 46 720.2 0 46 168 33 720.2 0 33 169 60 720.2 0 60 170 76 720.2 0 76 171 110 720.2 0 110 172 29 720.2 0 29 173 41 720.2 0 41 174 48 720.2 0 48 175 186 720.2 0 186 176 208 720.2 0 208 177 245 720.2 0 245 178 50 720.2 0 50 179 75 720.2 0 75 180 472 720.2 0 472 181 993 720.2 0 993 182 819 720.2 0 819 183 635 720.2 0 635 184 40 720.2 0 40 185 64 720.2 0 64 186 60 720.2 0 60 187 46 720.2 0 46 188 43 720.2 0 43 189 32 720.2 0 32 190 42 720.2 0 42 191 46 720.2 0 46 192 45 720.2 0 45 193 28 720.2 0 28 194 27 720.2 0 27 195 43 720.2 0 43 196 47 720.2 0 47 197 44 720.2 0 44 198 42 720.2 0 42 199 49 720.2 0 49 200 48 720.2 0 48 201 25 720.2 0 25 202 32 720.2 0 32 203 41 720.2 0 41 204 28 720.2 0 28 205 37 720.2 0 37 206 41 720.2 0 41 207 48 720.2 0 48 208 60 720.2 0 60 209 69 720.2 0 69 210 86 720.2 0 86 211 91 720.2 0 91 212 1604 720.2 0 1604 213 478 720.2 0 478 214 2293 720.2 0 2293 215 65 720.2 0 65 216 41 720.2 0 41 217 56 720.2 0 56 218 80 720.2 0 80 219 65 720.2 0 65 220 57 720.2 0 57 221 60 720.2 0 60 222 52 720.2 0 52 223 36 720.2 0 36 224 61 720.2 0 61 225 82 720.2 0 82 226 107 720.2 0 107 227 60 720.2 0 60 228 48 720.2 0 48 229 52 720.2 0 52 230 47 720.2 0 47 231 24 720.2 0 24 232 35 720.2 0 35 233 58 720.2 0 58 234 55 720.2 0 55 235 49 720.2 0 49 236 56 720.2 0 56 237 58 720.2 0 58 238 123 720.2 0 123 239 196 720.2 0 196 240 87 720.2 0 87 241 105 720.2 0 105 242 51 720.2 0 51 243 84 720.2 0 84 244 73 720.2 0 73 245 86 720.2 0 86 246 77 720.2 0 77 247 61 720.2 0 61 248 120 720.2 0 120 249 77 720.2 0 77 250 43 720.2 0 43 251 56 720.2 0 56 252 69 720.2 0 69 253 58 720.2 0 58 254 82 720.2 0 82 255 107 720.2 0 107 256 290 720.2 0 290 257 486 720.2 0 486 258 611 720.2 0 611 259 194 720.2 0 194 260 138 720.2 0 138 261 73 720.2 0 73 262 65 720.2 0 65 263 45 720.2 0 45 264 56 720.2 0 56 265 55 720.2 0 55 266 143 720.2 0 143 267 199 720.2 0 199 268 426 720.2 0 426 269 149 720.2 0 149 270 108 720.2 0 108 271 126 720.2 0 126 272 233 720.2 0 233 273 648 720.2 0 648 274 596 720.2 0 596 275 650 720.2 0 650 276 209 720.2 0 209 277 177 720.2 0 177 278 191 720.2 0 191 279 90 720.2 0 90 280 156 720.2 0 156 281 161 720.2 0 161 282 114 720.2 0 114 283 84 720.2 0 84 284 99 720.2 0 99 285 123 720.2 0 123 286 113 720.2 0 113 287 171 720.2 0 171 288 189 720.2 0 189 289 181 720.2 0 181 290 55 720.2 0 55 291 74 720.2 0 74 292 89 720.2 0 89 293 159 720.2 0 159 294 112 720.2 0 112 295 76 720.2 0 76 296 49 720.2 0 49 297 74 720.2 0 74 298 107 720.2 0 107 299 95 720.2 0 95 300 77 720.2 0 77 301 101 720.2 0 101 Finished in 2.06 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 41,888 Reads with adapters: 140 (0.3%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 108 (0.3%) Total basepairs processed: 6,745,217 bp Total written (filtered): 28,002 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 140 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 1 17 95 0.0 3 93 2 23 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 1 99 2 0.0 3 1 1 100 2 0.0 3 1 1 113 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 166 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 2 0.0 3 2 205 1 0.0 3 1 206 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (93 us/read; 0.65 M reads/minute). === Summary === Total reads processed: 108 Reads with adapters: 6 (5.6%) Reads written (passing filters): 108 (100.0%) Total basepairs processed: 28,002 bp Total written (filtered): 27,982 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.3% C: 16.7% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 1.7 0 4 4 2 0.4 0 2