This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.85 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 53,966 (2.4%) Reads that were too short: 29,284 (1.3%) Reads written (passing filters): 24,682 (1.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 5,370,096 bp (0.8%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 53966 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 181 546.6 0 181 7 2829 546.6 0 2829 8 49 546.6 0 49 9 3080 546.6 0 3080 10 12 546.6 0 12 11 118 546.6 0 118 12 59 546.6 0 59 13 4 546.6 0 4 14 3 546.6 0 3 15 5 546.6 0 5 16 7 546.6 0 7 17 10 546.6 0 10 18 6 546.6 0 6 19 7 546.6 0 7 20 5 546.6 0 5 21 4 546.6 0 4 22 7 546.6 0 7 23 8 546.6 0 8 24 18 546.6 0 18 25 13 546.6 0 13 26 15 546.6 0 15 27 516 546.6 0 516 28 45 546.6 0 45 29 124 546.6 0 124 30 9 546.6 0 9 31 5 546.6 0 5 32 7 546.6 0 7 33 5 546.6 0 5 34 8 546.6 0 8 35 4 546.6 0 4 36 8 546.6 0 8 37 4 546.6 0 4 38 10 546.6 0 10 39 14 546.6 0 14 40 17 546.6 0 17 41 15 546.6 0 15 42 20 546.6 0 20 43 4 546.6 0 4 44 10 546.6 0 10 45 7 546.6 0 7 46 15 546.6 0 15 47 21 546.6 0 21 48 7 546.6 0 7 49 11 546.6 0 11 50 41 546.6 0 41 51 16 546.6 0 16 52 26 546.6 0 26 53 44 546.6 0 44 54 21 546.6 0 21 55 31 546.6 0 31 56 7 546.6 0 7 57 12 546.6 0 12 58 29 546.6 0 29 59 24 546.6 0 24 60 56 546.6 0 56 61 109 546.6 0 109 62 443 546.6 0 443 63 439 546.6 0 439 64 666 546.6 0 666 65 32 546.6 0 32 66 66 546.6 0 66 67 45 546.6 0 45 68 36 546.6 0 36 69 41 546.6 0 41 70 17 546.6 0 17 71 20 546.6 0 20 72 35 546.6 0 35 73 90 546.6 0 90 74 78 546.6 0 78 75 58 546.6 0 58 76 71 546.6 0 71 77 157 546.6 0 157 78 1786 546.6 0 1786 79 473 546.6 0 473 80 2011 546.6 0 2011 81 1900 546.6 0 1900 82 114 546.6 0 114 83 575 546.6 0 575 84 11 546.6 0 11 85 59 546.6 0 59 86 19 546.6 0 19 87 21 546.6 0 21 88 12 546.6 0 12 89 12 546.6 0 12 90 20 546.6 0 20 91 14 546.6 0 14 92 24 546.6 0 24 93 25 546.6 0 25 94 29 546.6 0 29 95 20 546.6 0 20 96 28 546.6 0 28 97 15 546.6 0 15 98 11 546.6 0 11 99 8 546.6 0 8 100 21 546.6 0 21 101 47 546.6 0 47 102 14 546.6 0 14 103 10 546.6 0 10 104 11 546.6 0 11 105 11 546.6 0 11 106 3 546.6 0 3 107 16 546.6 0 16 108 16 546.6 0 16 109 84 546.6 0 84 110 34 546.6 0 34 111 77 546.6 0 77 112 12 546.6 0 12 113 21 546.6 0 21 114 20 546.6 0 20 115 50 546.6 0 50 116 186 546.6 0 186 117 144 546.6 0 144 118 33 546.6 0 33 119 13 546.6 0 13 120 33 546.6 0 33 121 52 546.6 0 52 122 43 546.6 0 43 123 55 546.6 0 55 124 42 546.6 0 42 125 97 546.6 0 97 126 72 546.6 0 72 127 58 546.6 0 58 128 40 546.6 0 40 129 43 546.6 0 43 130 60 546.6 0 60 131 58 546.6 0 58 132 24 546.6 0 24 133 26 546.6 0 26 134 14 546.6 0 14 135 19 546.6 0 19 136 22 546.6 0 22 137 32 546.6 0 32 138 25 546.6 0 25 139 25 546.6 0 25 140 43 546.6 0 43 141 35 546.6 0 35 142 97 546.6 0 97 143 29 546.6 0 29 144 98 546.6 0 98 145 109 546.6 0 109 146 148 546.6 0 148 147 37 546.6 0 37 148 63 546.6 0 63 149 33 546.6 0 33 150 39 546.6 0 39 151 38 546.6 0 38 152 37 546.6 0 37 153 33 546.6 0 33 154 19 546.6 0 19 155 30 546.6 0 30 156 29 546.6 0 29 157 33 546.6 0 33 158 41 546.6 0 41 159 34 546.6 0 34 160 31 546.6 0 31 161 28 546.6 0 28 162 28 546.6 0 28 163 43 546.6 0 43 164 48 546.6 0 48 165 46 546.6 0 46 166 68 546.6 0 68 167 48 546.6 0 48 168 47 546.6 0 47 169 52 546.6 0 52 170 68 546.6 0 68 171 39 546.6 0 39 172 63 546.6 0 63 173 40 546.6 0 40 174 35 546.6 0 35 175 41 546.6 0 41 176 45 546.6 0 45 177 33 546.6 0 33 178 50 546.6 0 50 179 53 546.6 0 53 180 100 546.6 0 100 181 1342 546.6 0 1342 182 177 546.6 0 177 183 339 546.6 0 339 184 36 546.6 0 36 185 100 546.6 0 100 186 285 546.6 0 285 187 297 546.6 0 297 188 86 546.6 0 86 189 33 546.6 0 33 190 59 546.6 0 59 191 103 546.6 0 103 192 198 546.6 0 198 193 98 546.6 0 98 194 94 546.6 0 94 195 56 546.6 0 56 196 59 546.6 0 59 197 85 546.6 0 85 198 116 546.6 0 116 199 109 546.6 0 109 200 57 546.6 0 57 201 36 546.6 0 36 202 43 546.6 0 43 203 78 546.6 0 78 204 138 546.6 0 138 205 74 546.6 0 74 206 188 546.6 0 188 207 36 546.6 0 36 208 60 546.6 0 60 209 114 546.6 0 114 210 247 546.6 0 247 211 133 546.6 0 133 212 88 546.6 0 88 213 152 546.6 0 152 214 63 546.6 0 63 215 112 546.6 0 112 216 77 546.6 0 77 217 328 546.6 0 328 218 929 546.6 0 929 219 912 546.6 0 912 220 137 546.6 0 137 221 168 546.6 0 168 222 188 546.6 0 188 223 134 546.6 0 134 224 124 546.6 0 124 225 955 546.6 0 955 226 1277 546.6 0 1277 227 3361 546.6 0 3361 228 795 546.6 0 795 229 1263 546.6 0 1263 230 787 546.6 0 787 231 113 546.6 0 113 232 66 546.6 0 66 233 48 546.6 0 48 234 85 546.6 0 85 235 170 546.6 0 170 236 142 546.6 0 142 237 419 546.6 0 419 238 615 546.6 0 615 239 325 546.6 0 325 240 249 546.6 0 249 241 159 546.6 0 159 242 110 546.6 0 110 243 126 546.6 0 126 244 140 546.6 0 140 245 427 546.6 0 427 246 250 546.6 0 250 247 541 546.6 0 541 248 239 546.6 0 239 249 215 546.6 0 215 250 143 546.6 0 143 251 98 546.6 0 98 252 67 546.6 0 67 253 51 546.6 0 51 254 102 546.6 0 102 255 71 546.6 0 71 256 91 546.6 0 91 257 99 546.6 0 99 258 61 546.6 0 61 259 73 546.6 0 73 260 319 546.6 0 319 261 783 546.6 0 783 262 656 546.6 0 656 263 334 546.6 0 334 264 397 546.6 0 397 265 144 546.6 0 144 266 247 546.6 0 247 267 170 546.6 0 170 268 96 546.6 0 96 269 93 546.6 0 93 270 66 546.6 0 66 271 78 546.6 0 78 272 161 546.6 0 161 273 320 546.6 0 320 274 210 546.6 0 210 275 79 546.6 0 79 276 92 546.6 0 92 277 86 546.6 0 86 278 134 546.6 0 134 279 220 546.6 0 220 280 257 546.6 0 257 281 164 546.6 0 164 282 145 546.6 0 145 283 88 546.6 0 88 284 76 546.6 0 76 285 183 546.6 0 183 286 531 546.6 0 531 287 1891 546.6 0 1891 288 949 546.6 0 949 289 139 546.6 0 139 290 118 546.6 0 118 291 125 546.6 0 125 292 123 546.6 0 123 293 106 546.6 0 106 294 138 546.6 0 138 295 124 546.6 0 124 296 140 546.6 0 140 297 219 546.6 0 219 298 196 546.6 0 196 299 188 546.6 0 188 300 175 546.6 0 175 301 268 546.6 0 268 Finished in 1.09 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 24,682 Reads with adapters: 3,042 (12.3%) Reads that were too short: 14 (0.1%) Reads written (passing filters): 3,028 (12.3%) Total basepairs processed: 5,370,096 bp Total written (filtered): 820,541 bp (15.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3042 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 19 18 0.0 3 0 15 1 2 20 2914 0.0 4 2780 110 16 5 3 21 9 0.0 4 1 6 2 30 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 78 0.0 4 0 0 0 0 78 130 1 0.0 4 0 0 1 137 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 187 1 0.0 4 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,028 Reads with adapters: 238 (7.9%) Reads written (passing filters): 3,028 (100.0%) Total basepairs processed: 820,541 bp Total written (filtered): 814,617 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 238 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 35.7% C: 12.2% G: 50.0% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 47.3 0 14 4 3 11.8 0 3 10 19 0.0 1 0 19 11 90 0.0 1 6 84 12 36 0.0 1 1 35 20 57 0.0 1 39 18 21 5 0.0 1 5 210 12 0.0 1 11 1 225 1 0.0 1 0 1 268 1 0.0 1 1