This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.02 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 33,579 (1.5%) Reads that were too short: 14,083 (0.6%) Reads written (passing filters): 19,496 (0.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 4,442,711 bp (0.7%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 33579 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 58 546.6 0 58 7 3238 546.6 0 3238 8 43 546.6 0 43 9 3086 546.6 0 3086 10 3 546.6 0 3 11 40 546.6 0 40 12 14 546.6 0 14 13 5 546.6 0 5 14 6 546.6 0 6 15 2 546.6 0 2 16 6 546.6 0 6 17 2 546.6 0 2 18 7 546.6 0 7 19 4 546.6 0 4 20 4 546.6 0 4 21 1 546.6 0 1 22 7 546.6 0 7 23 4 546.6 0 4 24 7 546.6 0 7 25 13 546.6 0 13 26 12 546.6 0 12 27 584 546.6 0 584 28 18 546.6 0 18 29 110 546.6 0 110 30 6 546.6 0 6 31 8 546.6 0 8 32 4 546.6 0 4 33 4 546.6 0 4 34 7 546.6 0 7 35 4 546.6 0 4 36 5 546.6 0 5 37 3 546.6 0 3 38 10 546.6 0 10 39 14 546.6 0 14 40 18 546.6 0 18 41 11 546.6 0 11 42 24 546.6 0 24 43 10 546.6 0 10 44 9 546.6 0 9 45 8 546.6 0 8 46 9 546.6 0 9 47 24 546.6 0 24 48 14 546.6 0 14 49 9 546.6 0 9 50 53 546.6 0 53 51 21 546.6 0 21 52 29 546.6 0 29 53 16 546.6 0 16 54 11 546.6 0 11 55 20 546.6 0 20 56 4 546.6 0 4 57 7 546.6 0 7 58 11 546.6 0 11 59 19 546.6 0 19 60 26 546.6 0 26 61 22 546.6 0 22 62 193 546.6 0 193 63 194 546.6 0 194 64 223 546.6 0 223 65 11 546.6 0 11 66 30 546.6 0 30 67 40 546.6 0 40 68 15 546.6 0 15 69 34 546.6 0 34 70 4 546.6 0 4 71 4 546.6 0 4 72 14 546.6 0 14 73 32 546.6 0 32 74 40 546.6 0 40 75 33 546.6 0 33 76 36 546.6 0 36 77 99 546.6 0 99 78 1031 546.6 0 1031 79 258 546.6 0 258 80 1783 546.6 0 1783 81 2255 546.6 0 2255 82 55 546.6 0 55 83 518 546.6 0 518 84 8 546.6 0 8 85 56 546.6 0 56 86 11 546.6 0 11 87 7 546.6 0 7 88 17 546.6 0 17 89 14 546.6 0 14 90 21 546.6 0 21 91 17 546.6 0 17 92 25 546.6 0 25 93 10 546.6 0 10 94 17 546.6 0 17 95 26 546.6 0 26 96 13 546.6 0 13 97 12 546.6 0 12 98 20 546.6 0 20 99 12 546.6 0 12 100 17 546.6 0 17 101 53 546.6 0 53 102 6 546.6 0 6 103 7 546.6 0 7 104 9 546.6 0 9 105 7 546.6 0 7 106 9 546.6 0 9 107 11 546.6 0 11 108 16 546.6 0 16 109 45 546.6 0 45 110 20 546.6 0 20 111 42 546.6 0 42 112 7 546.6 0 7 113 10 546.6 0 10 114 12 546.6 0 12 115 25 546.6 0 25 116 96 546.6 0 96 117 39 546.6 0 39 118 23 546.6 0 23 119 13 546.6 0 13 120 8 546.6 0 8 121 12 546.6 0 12 122 20 546.6 0 20 123 29 546.6 0 29 124 16 546.6 0 16 125 33 546.6 0 33 126 47 546.6 0 47 127 48 546.6 0 48 128 30 546.6 0 30 129 22 546.6 0 22 130 22 546.6 0 22 131 22 546.6 0 22 132 7 546.6 0 7 133 4 546.6 0 4 134 7 546.6 0 7 135 14 546.6 0 14 136 16 546.6 0 16 137 20 546.6 0 20 138 13 546.6 0 13 139 10 546.6 0 10 140 23 546.6 0 23 141 8 546.6 0 8 142 68 546.6 0 68 143 9 546.6 0 9 144 29 546.6 0 29 145 44 546.6 0 44 146 38 546.6 0 38 147 15 546.6 0 15 148 61 546.6 0 61 149 13 546.6 0 13 150 13 546.6 0 13 151 18 546.6 0 18 152 17 546.6 0 17 153 26 546.6 0 26 154 21 546.6 0 21 155 22 546.6 0 22 156 14 546.6 0 14 157 26 546.6 0 26 158 19 546.6 0 19 159 20 546.6 0 20 160 22 546.6 0 22 161 19 546.6 0 19 162 13 546.6 0 13 163 16 546.6 0 16 164 13 546.6 0 13 165 17 546.6 0 17 166 25 546.6 0 25 167 23 546.6 0 23 168 14 546.6 0 14 169 15 546.6 0 15 170 29 546.6 0 29 171 18 546.6 0 18 172 20 546.6 0 20 173 16 546.6 0 16 174 24 546.6 0 24 175 17 546.6 0 17 176 10 546.6 0 10 177 21 546.6 0 21 178 28 546.6 0 28 179 41 546.6 0 41 180 59 546.6 0 59 181 1566 546.6 0 1566 182 183 546.6 0 183 183 348 546.6 0 348 184 8 546.6 0 8 185 22 546.6 0 22 186 94 546.6 0 94 187 89 546.6 0 89 188 47 546.6 0 47 189 12 546.6 0 12 190 32 546.6 0 32 191 51 546.6 0 51 192 80 546.6 0 80 193 30 546.6 0 30 194 32 546.6 0 32 195 18 546.6 0 18 196 16 546.6 0 16 197 29 546.6 0 29 198 26 546.6 0 26 199 39 546.6 0 39 200 23 546.6 0 23 201 18 546.6 0 18 202 25 546.6 0 25 203 32 546.6 0 32 204 41 546.6 0 41 205 44 546.6 0 44 206 87 546.6 0 87 207 14 546.6 0 14 208 29 546.6 0 29 209 34 546.6 0 34 210 81 546.6 0 81 211 55 546.6 0 55 212 36 546.6 0 36 213 108 546.6 0 108 214 44 546.6 0 44 215 142 546.6 0 142 216 36 546.6 0 36 217 152 546.6 0 152 218 451 546.6 0 451 219 463 546.6 0 463 220 41 546.6 0 41 221 72 546.6 0 72 222 66 546.6 0 66 223 48 546.6 0 48 224 52 546.6 0 52 225 310 546.6 0 310 226 507 546.6 0 507 227 1068 546.6 0 1068 228 541 546.6 0 541 229 434 546.6 0 434 230 317 546.6 0 317 231 47 546.6 0 47 232 27 546.6 0 27 233 25 546.6 0 25 234 57 546.6 0 57 235 69 546.6 0 69 236 69 546.6 0 69 237 195 546.6 0 195 238 328 546.6 0 328 239 134 546.6 0 134 240 112 546.6 0 112 241 95 546.6 0 95 242 55 546.6 0 55 243 67 546.6 0 67 244 62 546.6 0 62 245 130 546.6 0 130 246 94 546.6 0 94 247 119 546.6 0 119 248 66 546.6 0 66 249 45 546.6 0 45 250 46 546.6 0 46 251 32 546.6 0 32 252 32 546.6 0 32 253 26 546.6 0 26 254 27 546.6 0 27 255 31 546.6 0 31 256 38 546.6 0 38 257 32 546.6 0 32 258 40 546.6 0 40 259 60 546.6 0 60 260 337 546.6 0 337 261 899 546.6 0 899 262 692 546.6 0 692 263 242 546.6 0 242 264 225 546.6 0 225 265 126 546.6 0 126 266 146 546.6 0 146 267 80 546.6 0 80 268 42 546.6 0 42 269 30 546.6 0 30 270 38 546.6 0 38 271 42 546.6 0 42 272 88 546.6 0 88 273 252 546.6 0 252 274 169 546.6 0 169 275 40 546.6 0 40 276 63 546.6 0 63 277 64 546.6 0 64 278 55 546.6 0 55 279 90 546.6 0 90 280 81 546.6 0 81 281 73 546.6 0 73 282 71 546.6 0 71 283 23 546.6 0 23 284 32 546.6 0 32 285 92 546.6 0 92 286 235 546.6 0 235 287 971 546.6 0 971 288 456 546.6 0 456 289 55 546.6 0 55 290 52 546.6 0 52 291 47 546.6 0 47 292 45 546.6 0 45 293 55 546.6 0 55 294 82 546.6 0 82 295 40 546.6 0 40 296 74 546.6 0 74 297 112 546.6 0 112 298 61 546.6 0 61 299 82 546.6 0 82 300 48 546.6 0 48 301 86 546.6 0 86 Finished in 0.83 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 19,496 Reads with adapters: 3,203 (16.4%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 3,180 (16.3%) Total basepairs processed: 4,442,711 bp Total written (filtered): 870,355 bp (19.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3203 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 12 0.0 3 0 12 17 3111 0.0 3 3040 68 2 1 18 8 0.0 3 1 7 19 1 0.0 3 0 0 1 20 5 0.0 3 0 0 0 5 22 19 0.0 3 0 0 0 19 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 115 1 0.0 3 1 126 1 0.0 3 1 129 5 0.0 3 0 0 0 5 130 3 0.0 3 0 0 0 3 133 4 0.0 3 0 0 0 4 144 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 204 2 0.0 3 2 209 7 0.0 3 0 0 0 7 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 212 2 0.0 3 0 0 0 2 242 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 3,180 Reads with adapters: 38 (1.2%) Reads written (passing filters): 3,180 (100.0%) Total basepairs processed: 870,355 bp Total written (filtered): 870,233 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 38 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.8% C: 13.2% G: 47.4% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 30 49.7 0 30 4 8 12.4 0 8