This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.18 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 82,197 (3.7%) Reads that were too short: 12,681 (0.6%) Reads written (passing filters): 69,516 (3.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 16,382,458 bp (2.4%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 82197 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 188 546.6 0 188 7 318 546.6 0 318 8 15197 546.6 0 15197 9 13237 546.6 0 13237 10 44 546.6 0 44 11 34 546.6 0 34 12 33 546.6 0 33 13 71 546.6 0 71 14 14 546.6 0 14 15 25 546.6 0 25 16 21 546.6 0 21 17 28 546.6 0 28 18 29 546.6 0 29 19 22 546.6 0 22 20 19 546.6 0 19 21 18 546.6 0 18 22 20 546.6 0 20 23 23 546.6 0 23 24 21 546.6 0 21 25 30 546.6 0 30 26 25 546.6 0 25 27 36 546.6 0 36 28 23 546.6 0 23 29 32 546.6 0 32 30 32 546.6 0 32 31 170 546.6 0 170 32 137 546.6 0 137 33 136 546.6 0 136 34 32 546.6 0 32 35 45 546.6 0 45 36 20 546.6 0 20 37 46 546.6 0 46 38 44 546.6 0 44 39 59 546.6 0 59 40 31 546.6 0 31 41 33 546.6 0 33 42 43 546.6 0 43 43 40 546.6 0 40 44 24 546.6 0 24 45 22 546.6 0 22 46 36 546.6 0 36 47 44 546.6 0 44 48 52 546.6 0 52 49 60 546.6 0 60 50 41 546.6 0 41 51 110 546.6 0 110 52 35 546.6 0 35 53 55 546.6 0 55 54 38 546.6 0 38 55 42 546.6 0 42 56 58 546.6 0 58 57 43 546.6 0 43 58 46 546.6 0 46 59 44 546.6 0 44 60 26 546.6 0 26 61 28 546.6 0 28 62 32 546.6 0 32 63 32 546.6 0 32 64 38 546.6 0 38 65 35 546.6 0 35 66 55 546.6 0 55 67 54 546.6 0 54 68 52 546.6 0 52 69 44 546.6 0 44 70 34 546.6 0 34 71 38 546.6 0 38 72 45 546.6 0 45 73 40 546.6 0 40 74 41 546.6 0 41 75 43 546.6 0 43 76 79 546.6 0 79 77 53 546.6 0 53 78 42 546.6 0 42 79 36 546.6 0 36 80 54 546.6 0 54 81 34 546.6 0 34 82 59 546.6 0 59 83 47 546.6 0 47 84 58 546.6 0 58 85 120 546.6 0 120 86 868 546.6 0 868 87 185 546.6 0 185 88 383 546.6 0 383 89 92 546.6 0 92 90 214 546.6 0 214 91 80 546.6 0 80 92 52 546.6 0 52 93 60 546.6 0 60 94 60 546.6 0 60 95 59 546.6 0 59 96 90 546.6 0 90 97 55 546.6 0 55 98 115 546.6 0 115 99 151 546.6 0 151 100 214 546.6 0 214 101 399 546.6 0 399 102 1048 546.6 0 1048 103 17900 546.6 0 17900 104 2170 546.6 0 2170 105 5685 546.6 0 5685 106 468 546.6 0 468 107 497 546.6 0 497 108 372 546.6 0 372 109 41 546.6 0 41 110 54 546.6 0 54 111 44 546.6 0 44 112 94 546.6 0 94 113 59 546.6 0 59 114 29 546.6 0 29 115 15 546.6 0 15 116 21 546.6 0 21 117 32 546.6 0 32 118 48 546.6 0 48 119 44 546.6 0 44 120 39 546.6 0 39 121 30 546.6 0 30 122 31 546.6 0 31 123 39 546.6 0 39 124 44 546.6 0 44 125 47 546.6 0 47 126 48 546.6 0 48 127 55 546.6 0 55 128 40 546.6 0 40 129 54 546.6 0 54 130 30 546.6 0 30 131 52 546.6 0 52 132 53 546.6 0 53 133 45 546.6 0 45 134 45 546.6 0 45 135 64 546.6 0 64 136 37 546.6 0 37 137 37 546.6 0 37 138 58 546.6 0 58 139 127 546.6 0 127 140 114 546.6 0 114 141 72 546.6 0 72 142 57 546.6 0 57 143 262 546.6 0 262 144 74 546.6 0 74 145 188 546.6 0 188 146 49 546.6 0 49 147 41 546.6 0 41 148 55 546.6 0 55 149 129 546.6 0 129 150 244 546.6 0 244 151 164 546.6 0 164 152 114 546.6 0 114 153 115 546.6 0 115 154 68 546.6 0 68 155 81 546.6 0 81 156 61 546.6 0 61 157 56 546.6 0 56 158 69 546.6 0 69 159 75 546.6 0 75 160 89 546.6 0 89 161 58 546.6 0 58 162 33 546.6 0 33 163 26 546.6 0 26 164 43 546.6 0 43 165 48 546.6 0 48 166 72 546.6 0 72 167 45 546.6 0 45 168 29 546.6 0 29 169 33 546.6 0 33 170 38 546.6 0 38 171 50 546.6 0 50 172 62 546.6 0 62 173 66 546.6 0 66 174 25 546.6 0 25 175 38 546.6 0 38 176 39 546.6 0 39 177 33 546.6 0 33 178 102 546.6 0 102 179 92 546.6 0 92 180 158 546.6 0 158 181 72 546.6 0 72 182 47 546.6 0 47 183 53 546.6 0 53 184 54 546.6 0 54 185 126 546.6 0 126 186 60 546.6 0 60 187 53 546.6 0 53 188 42 546.6 0 42 189 89 546.6 0 89 190 55 546.6 0 55 191 123 546.6 0 123 192 40 546.6 0 40 193 53 546.6 0 53 194 25 546.6 0 25 195 49 546.6 0 49 196 41 546.6 0 41 197 53 546.6 0 53 198 37 546.6 0 37 199 46 546.6 0 46 200 59 546.6 0 59 201 54 546.6 0 54 202 49 546.6 0 49 203 43 546.6 0 43 204 58 546.6 0 58 205 129 546.6 0 129 206 172 546.6 0 172 207 156 546.6 0 156 208 101 546.6 0 101 209 65 546.6 0 65 210 63 546.6 0 63 211 128 546.6 0 128 212 73 546.6 0 73 213 104 546.6 0 104 214 120 546.6 0 120 215 99 546.6 0 99 216 106 546.6 0 106 217 97 546.6 0 97 218 88 546.6 0 88 219 107 546.6 0 107 220 89 546.6 0 89 221 152 546.6 0 152 222 61 546.6 0 61 223 97 546.6 0 97 224 89 546.6 0 89 225 108 546.6 0 108 226 66 546.6 0 66 227 70 546.6 0 70 228 40 546.6 0 40 229 61 546.6 0 61 230 53 546.6 0 53 231 70 546.6 0 70 232 53 546.6 0 53 233 84 546.6 0 84 234 147 546.6 0 147 235 157 546.6 0 157 236 91 546.6 0 91 237 56 546.6 0 56 238 55 546.6 0 55 239 67 546.6 0 67 240 73 546.6 0 73 241 86 546.6 0 86 242 72 546.6 0 72 243 74 546.6 0 74 244 51 546.6 0 51 245 70 546.6 0 70 246 41 546.6 0 41 247 74 546.6 0 74 248 56 546.6 0 56 249 25 546.6 0 25 250 48 546.6 0 48 251 72 546.6 0 72 252 76 546.6 0 76 253 50 546.6 0 50 254 67 546.6 0 67 255 68 546.6 0 68 256 91 546.6 0 91 257 128 546.6 0 128 258 99 546.6 0 99 259 376 546.6 0 376 260 312 546.6 0 312 261 798 546.6 0 798 262 1238 546.6 0 1238 263 1473 546.6 0 1473 264 79 546.6 0 79 265 66 546.6 0 66 266 51 546.6 0 51 267 91 546.6 0 91 268 60 546.6 0 60 269 27 546.6 0 27 270 45 546.6 0 45 271 55 546.6 0 55 272 76 546.6 0 76 273 66 546.6 0 66 274 149 546.6 0 149 275 69 546.6 0 69 276 76 546.6 0 76 277 69 546.6 0 69 278 88 546.6 0 88 279 183 546.6 0 183 280 273 546.6 0 273 281 272 546.6 0 272 282 246 546.6 0 246 283 104 546.6 0 104 284 191 546.6 0 191 285 55 546.6 0 55 286 64 546.6 0 64 287 81 546.6 0 81 288 79 546.6 0 79 289 62 546.6 0 62 290 62 546.6 0 62 291 46 546.6 0 46 292 53 546.6 0 53 293 118 546.6 0 118 294 88 546.6 0 88 295 93 546.6 0 93 296 68 546.6 0 68 297 85 546.6 0 85 298 66 546.6 0 66 299 90 546.6 0 90 300 121 546.6 0 121 301 172 546.6 0 172 Finished in 3.16 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 69,516 Reads with adapters: 13,838 (19.9%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 13,822 (19.9%) Total basepairs processed: 16,382,458 bp Total written (filtered): 3,734,524 bp (22.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13838 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 8 0.0 3 0 0 1 4 3 19 77 0.0 3 0 46 9 16 6 20 13387 0.0 4 12468 711 141 51 16 21 38 0.0 4 8 28 1 0 1 22 3 0.0 4 0 0 1 2 23 65 0.0 4 0 0 0 45 20 28 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 1 1 44 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 1 0 0 0 1 127 223 0.0 4 0 0 0 0 223 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 1 0 0 1 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 192 1 0.0 4 1 222 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 1 228 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 13,822 Reads with adapters: 3,543 (25.6%) Reads written (passing filters): 13,822 (100.0%) Total basepairs processed: 3,734,524 bp Total written (filtered): 3,688,887 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3543 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 9.7% G: 84.1% T: 3.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 216.0 0 2 4 1 54.0 0 1 8 1 0.2 0 1 9 854 0.1 0 110 744 10 2228 0.0 1 200 2028 11 386 0.0 1 19 367 12 12 0.0 1 0 12 13 6 0.0 1 0 6 14 1 0.0 1 0 1 150 3 0.0 1 3 208 12 0.0 1 10 2 209 1 0.0 1 1 211 1 0.0 1 1 223 35 0.0 1 30 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.