This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.86 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 76,696 (3.4%) Reads that were too short: 7,815 (0.3%) Reads written (passing filters): 68,881 (3.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 16,348,635 bp (2.4%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 76696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 153 546.6 0 153 7 289 546.6 0 289 8 13669 546.6 0 13669 9 15724 546.6 0 15724 10 33 546.6 0 33 11 18 546.6 0 18 12 18 546.6 0 18 13 16 546.6 0 16 14 23 546.6 0 23 15 21 546.6 0 21 16 22 546.6 0 22 17 18 546.6 0 18 18 27 546.6 0 27 19 11 546.6 0 11 20 14 546.6 0 14 21 17 546.6 0 17 22 25 546.6 0 25 23 21 546.6 0 21 24 9 546.6 0 9 25 17 546.6 0 17 26 26 546.6 0 26 27 14 546.6 0 14 28 16 546.6 0 16 29 26 546.6 0 26 30 19 546.6 0 19 31 18 546.6 0 18 32 21 546.6 0 21 33 29 546.6 0 29 34 17 546.6 0 17 35 20 546.6 0 20 36 14 546.6 0 14 37 20 546.6 0 20 38 20 546.6 0 20 39 33 546.6 0 33 40 14 546.6 0 14 41 20 546.6 0 20 42 29 546.6 0 29 43 27 546.6 0 27 44 16 546.6 0 16 45 19 546.6 0 19 46 24 546.6 0 24 47 26 546.6 0 26 48 28 546.6 0 28 49 22 546.6 0 22 50 32 546.6 0 32 51 123 546.6 0 123 52 27 546.6 0 27 53 32 546.6 0 32 54 19 546.6 0 19 55 22 546.6 0 22 56 15 546.6 0 15 57 32 546.6 0 32 58 27 546.6 0 27 59 33 546.6 0 33 60 23 546.6 0 23 61 24 546.6 0 24 62 27 546.6 0 27 63 20 546.6 0 20 64 18 546.6 0 18 65 18 546.6 0 18 66 33 546.6 0 33 67 30 546.6 0 30 68 25 546.6 0 25 69 34 546.6 0 34 70 27 546.6 0 27 71 26 546.6 0 26 72 26 546.6 0 26 73 38 546.6 0 38 74 26 546.6 0 26 75 15 546.6 0 15 76 50 546.6 0 50 77 20 546.6 0 20 78 17 546.6 0 17 79 22 546.6 0 22 80 22 546.6 0 22 81 21 546.6 0 21 82 25 546.6 0 25 83 37 546.6 0 37 84 32 546.6 0 32 85 83 546.6 0 83 86 444 546.6 0 444 87 149 546.6 0 149 88 279 546.6 0 279 89 54 546.6 0 54 90 126 546.6 0 126 91 83 546.6 0 83 92 52 546.6 0 52 93 43 546.6 0 43 94 43 546.6 0 43 95 36 546.6 0 36 96 66 546.6 0 66 97 56 546.6 0 56 98 87 546.6 0 87 99 153 546.6 0 153 100 160 546.6 0 160 101 359 546.6 0 359 102 906 546.6 0 906 103 20221 546.6 0 20221 104 2610 546.6 0 2610 105 6594 546.6 0 6594 106 278 546.6 0 278 107 222 546.6 0 222 108 157 546.6 0 157 109 40 546.6 0 40 110 22 546.6 0 22 111 30 546.6 0 30 112 29 546.6 0 29 113 26 546.6 0 26 114 25 546.6 0 25 115 17 546.6 0 17 116 19 546.6 0 19 117 14 546.6 0 14 118 27 546.6 0 27 119 26 546.6 0 26 120 27 546.6 0 27 121 22 546.6 0 22 122 13 546.6 0 13 123 18 546.6 0 18 124 21 546.6 0 21 125 22 546.6 0 22 126 35 546.6 0 35 127 37 546.6 0 37 128 21 546.6 0 21 129 27 546.6 0 27 130 19 546.6 0 19 131 28 546.6 0 28 132 33 546.6 0 33 133 42 546.6 0 42 134 46 546.6 0 46 135 41 546.6 0 41 136 19 546.6 0 19 137 13 546.6 0 13 138 20 546.6 0 20 139 81 546.6 0 81 140 30 546.6 0 30 141 28 546.6 0 28 142 23 546.6 0 23 143 227 546.6 0 227 144 36 546.6 0 36 145 98 546.6 0 98 146 24 546.6 0 24 147 22 546.6 0 22 148 28 546.6 0 28 149 97 546.6 0 97 150 191 546.6 0 191 151 132 546.6 0 132 152 115 546.6 0 115 153 114 546.6 0 114 154 52 546.6 0 52 155 78 546.6 0 78 156 37 546.6 0 37 157 34 546.6 0 34 158 55 546.6 0 55 159 46 546.6 0 46 160 51 546.6 0 51 161 24 546.6 0 24 162 28 546.6 0 28 163 15 546.6 0 15 164 27 546.6 0 27 165 27 546.6 0 27 166 30 546.6 0 30 167 22 546.6 0 22 168 23 546.6 0 23 169 23 546.6 0 23 170 35 546.6 0 35 171 37 546.6 0 37 172 36 546.6 0 36 173 53 546.6 0 53 174 38 546.6 0 38 175 20 546.6 0 20 176 20 546.6 0 20 177 15 546.6 0 15 178 80 546.6 0 80 179 53 546.6 0 53 180 145 546.6 0 145 181 47 546.6 0 47 182 28 546.6 0 28 183 34 546.6 0 34 184 46 546.6 0 46 185 58 546.6 0 58 186 26 546.6 0 26 187 30 546.6 0 30 188 27 546.6 0 27 189 37 546.6 0 37 190 44 546.6 0 44 191 133 546.6 0 133 192 26 546.6 0 26 193 26 546.6 0 26 194 20 546.6 0 20 195 29 546.6 0 29 196 19 546.6 0 19 197 51 546.6 0 51 198 23 546.6 0 23 199 36 546.6 0 36 200 38 546.6 0 38 201 32 546.6 0 32 202 40 546.6 0 40 203 23 546.6 0 23 204 36 546.6 0 36 205 93 546.6 0 93 206 122 546.6 0 122 207 94 546.6 0 94 208 50 546.6 0 50 209 28 546.6 0 28 210 44 546.6 0 44 211 65 546.6 0 65 212 40 546.6 0 40 213 51 546.6 0 51 214 57 546.6 0 57 215 45 546.6 0 45 216 44 546.6 0 44 217 33 546.6 0 33 218 32 546.6 0 32 219 53 546.6 0 53 220 50 546.6 0 50 221 65 546.6 0 65 222 42 546.6 0 42 223 48 546.6 0 48 224 71 546.6 0 71 225 65 546.6 0 65 226 45 546.6 0 45 227 35 546.6 0 35 228 23 546.6 0 23 229 32 546.6 0 32 230 30 546.6 0 30 231 44 546.6 0 44 232 39 546.6 0 39 233 32 546.6 0 32 234 53 546.6 0 53 235 56 546.6 0 56 236 31 546.6 0 31 237 29 546.6 0 29 238 41 546.6 0 41 239 37 546.6 0 37 240 54 546.6 0 54 241 63 546.6 0 63 242 51 546.6 0 51 243 50 546.6 0 50 244 35 546.6 0 35 245 35 546.6 0 35 246 28 546.6 0 28 247 42 546.6 0 42 248 40 546.6 0 40 249 28 546.6 0 28 250 35 546.6 0 35 251 28 546.6 0 28 252 46 546.6 0 46 253 32 546.6 0 32 254 40 546.6 0 40 255 40 546.6 0 40 256 48 546.6 0 48 257 80 546.6 0 80 258 62 546.6 0 62 259 203 546.6 0 203 260 189 546.6 0 189 261 612 546.6 0 612 262 1219 546.6 0 1219 263 1052 546.6 0 1052 264 39 546.6 0 39 265 25 546.6 0 25 266 22 546.6 0 22 267 44 546.6 0 44 268 33 546.6 0 33 269 25 546.6 0 25 270 30 546.6 0 30 271 20 546.6 0 20 272 32 546.6 0 32 273 25 546.6 0 25 274 77 546.6 0 77 275 45 546.6 0 45 276 37 546.6 0 37 277 29 546.6 0 29 278 45 546.6 0 45 279 100 546.6 0 100 280 119 546.6 0 119 281 124 546.6 0 124 282 77 546.6 0 77 283 62 546.6 0 62 284 99 546.6 0 99 285 37 546.6 0 37 286 36 546.6 0 36 287 44 546.6 0 44 288 54 546.6 0 54 289 35 546.6 0 35 290 29 546.6 0 29 291 17 546.6 0 17 292 27 546.6 0 27 293 77 546.6 0 77 294 43 546.6 0 43 295 66 546.6 0 66 296 57 546.6 0 57 297 29 546.6 0 29 298 40 546.6 0 40 299 53 546.6 0 53 300 51 546.6 0 51 301 81 546.6 0 81 Finished in 2.89 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 68,881 Reads with adapters: 13,634 (19.8%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 13,617 (19.8%) Total basepairs processed: 16,348,635 bp Total written (filtered): 3,756,216 bp (23.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13634 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 50 0.0 3 0 50 17 13485 0.0 3 13199 271 6 9 18 33 0.0 3 2 28 0 3 19 13 0.0 3 0 0 12 1 20 21 0.0 3 0 0 1 20 44 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 13,617 Reads with adapters: 15 (0.1%) Reads written (passing filters): 13,617 (100.0%) Total basepairs processed: 3,756,216 bp Total written (filtered): 3,756,159 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 15 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.3% C: 6.7% G: 20.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 212.8 0 6 4 7 53.2 0 7 5 1 13.3 0 1 6 1 3.3 0 1