This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.54 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 143,394 (6.4%) Reads that were too short: 45,019 (2.0%) Reads written (passing filters): 98,375 (4.4%) Total basepairs processed: 673,916,425 bp Total written (filtered): 22,965,656 bp (3.4%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 143394 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 236 546.6 0 236 7 10565 546.6 0 10565 8 7602 546.6 0 7602 9 40 546.6 0 40 10 46 546.6 0 46 11 46 546.6 0 46 12 16 546.6 0 16 13 36 546.6 0 36 14 23 546.6 0 23 15 20 546.6 0 20 16 47 546.6 0 47 17 27 546.6 0 27 18 44 546.6 0 44 19 61 546.6 0 61 20 16712 546.6 0 16712 21 9111 546.6 0 9111 22 149 546.6 0 149 23 103 546.6 0 103 24 45 546.6 0 45 25 42 546.6 0 42 26 172 546.6 0 172 27 26 546.6 0 26 28 18 546.6 0 18 29 42 546.6 0 42 30 23 546.6 0 23 31 38 546.6 0 38 32 26 546.6 0 26 33 7159 546.6 0 7159 34 767 546.6 0 767 35 38 546.6 0 38 36 37 546.6 0 37 37 19 546.6 0 19 38 53 546.6 0 53 39 57 546.6 0 57 40 39 546.6 0 39 41 39 546.6 0 39 42 35 546.6 0 35 43 49 546.6 0 49 44 53 546.6 0 53 45 42 546.6 0 42 46 48 546.6 0 48 47 51 546.6 0 51 48 89 546.6 0 89 49 74 546.6 0 74 50 85 546.6 0 85 51 24 546.6 0 24 52 44 546.6 0 44 53 44 546.6 0 44 54 41 546.6 0 41 55 50 546.6 0 50 56 59 546.6 0 59 57 52 546.6 0 52 58 55 546.6 0 55 59 49 546.6 0 49 60 64 546.6 0 64 61 65 546.6 0 65 62 61 546.6 0 61 63 92 546.6 0 92 64 61 546.6 0 61 65 96 546.6 0 96 66 85 546.6 0 85 67 98 546.6 0 98 68 87 546.6 0 87 69 208 546.6 0 208 70 133 546.6 0 133 71 164 546.6 0 164 72 108 546.6 0 108 73 128 546.6 0 128 74 177 546.6 0 177 75 458 546.6 0 458 76 670 546.6 0 670 77 3195 546.6 0 3195 78 1146 546.6 0 1146 79 158 546.6 0 158 80 292 546.6 0 292 81 428 546.6 0 428 82 457 546.6 0 457 83 94 546.6 0 94 84 98 546.6 0 98 85 140 546.6 0 140 86 145 546.6 0 145 87 153 546.6 0 153 88 829 546.6 0 829 89 147 546.6 0 147 90 465 546.6 0 465 91 133 546.6 0 133 92 121 546.6 0 121 93 126 546.6 0 126 94 131 546.6 0 131 95 139 546.6 0 139 96 102 546.6 0 102 97 137 546.6 0 137 98 114 546.6 0 114 99 130 546.6 0 130 100 169 546.6 0 169 101 190 546.6 0 190 102 114 546.6 0 114 103 116 546.6 0 116 104 136 546.6 0 136 105 121 546.6 0 121 106 118 546.6 0 118 107 136 546.6 0 136 108 177 546.6 0 177 109 157 546.6 0 157 110 167 546.6 0 167 111 192 546.6 0 192 112 178 546.6 0 178 113 168 546.6 0 168 114 166 546.6 0 166 115 181 546.6 0 181 116 243 546.6 0 243 117 195 546.6 0 195 118 169 546.6 0 169 119 196 546.6 0 196 120 196 546.6 0 196 121 175 546.6 0 175 122 209 546.6 0 209 123 293 546.6 0 293 124 281 546.6 0 281 125 241 546.6 0 241 126 200 546.6 0 200 127 231 546.6 0 231 128 234 546.6 0 234 129 284 546.6 0 284 130 1085 546.6 0 1085 131 2048 546.6 0 2048 132 2999 546.6 0 2999 133 262 546.6 0 262 134 269 546.6 0 269 135 324 546.6 0 324 136 1002 546.6 0 1002 137 1914 546.6 0 1914 138 2213 546.6 0 2213 139 1461 546.6 0 1461 140 513 546.6 0 513 141 280 546.6 0 280 142 182 546.6 0 182 143 161 546.6 0 161 144 135 546.6 0 135 145 157 546.6 0 157 146 183 546.6 0 183 147 182 546.6 0 182 148 242 546.6 0 242 149 192 546.6 0 192 150 165 546.6 0 165 151 200 546.6 0 200 152 257 546.6 0 257 153 220 546.6 0 220 154 178 546.6 0 178 155 148 546.6 0 148 156 135 546.6 0 135 157 139 546.6 0 139 158 181 546.6 0 181 159 130 546.6 0 130 160 130 546.6 0 130 161 189 546.6 0 189 162 230 546.6 0 230 163 188 546.6 0 188 164 224 546.6 0 224 165 210 546.6 0 210 166 228 546.6 0 228 167 140 546.6 0 140 168 148 546.6 0 148 169 125 546.6 0 125 170 259 546.6 0 259 171 1101 546.6 0 1101 172 157 546.6 0 157 173 145 546.6 0 145 174 141 546.6 0 141 175 153 546.6 0 153 176 173 546.6 0 173 177 160 546.6 0 160 178 145 546.6 0 145 179 210 546.6 0 210 180 148 546.6 0 148 181 161 546.6 0 161 182 125 546.6 0 125 183 170 546.6 0 170 184 185 546.6 0 185 185 196 546.6 0 196 186 180 546.6 0 180 187 227 546.6 0 227 188 183 546.6 0 183 189 478 546.6 0 478 190 231 546.6 0 231 191 206 546.6 0 206 192 191 546.6 0 191 193 199 546.6 0 199 194 188 546.6 0 188 195 242 546.6 0 242 196 382 546.6 0 382 197 301 546.6 0 301 198 209 546.6 0 209 199 143 546.6 0 143 200 143 546.6 0 143 201 138 546.6 0 138 202 153 546.6 0 153 203 164 546.6 0 164 204 161 546.6 0 161 205 171 546.6 0 171 206 179 546.6 0 179 207 175 546.6 0 175 208 178 546.6 0 178 209 224 546.6 0 224 210 169 546.6 0 169 211 207 546.6 0 207 212 190 546.6 0 190 213 159 546.6 0 159 214 208 546.6 0 208 215 187 546.6 0 187 216 189 546.6 0 189 217 186 546.6 0 186 218 191 546.6 0 191 219 191 546.6 0 191 220 192 546.6 0 192 221 209 546.6 0 209 222 313 546.6 0 313 223 280 546.6 0 280 224 319 546.6 0 319 225 328 546.6 0 328 226 931 546.6 0 931 227 406 546.6 0 406 228 413 546.6 0 413 229 490 546.6 0 490 230 550 546.6 0 550 231 360 546.6 0 360 232 521 546.6 0 521 233 491 546.6 0 491 234 187 546.6 0 187 235 220 546.6 0 220 236 242 546.6 0 242 237 231 546.6 0 231 238 250 546.6 0 250 239 462 546.6 0 462 240 356 546.6 0 356 241 370 546.6 0 370 242 460 546.6 0 460 243 481 546.6 0 481 244 569 546.6 0 569 245 360 546.6 0 360 246 483 546.6 0 483 247 515 546.6 0 515 248 444 546.6 0 444 249 337 546.6 0 337 250 544 546.6 0 544 251 2470 546.6 0 2470 252 3241 546.6 0 3241 253 8993 546.6 0 8993 254 456 546.6 0 456 255 212 546.6 0 212 256 357 546.6 0 357 257 358 546.6 0 358 258 1033 546.6 0 1033 259 415 546.6 0 415 260 911 546.6 0 911 261 633 546.6 0 633 262 285 546.6 0 285 263 221 546.6 0 221 264 318 546.6 0 318 265 294 546.6 0 294 266 205 546.6 0 205 267 206 546.6 0 206 268 170 546.6 0 170 269 214 546.6 0 214 270 238 546.6 0 238 271 275 546.6 0 275 272 251 546.6 0 251 273 247 546.6 0 247 274 241 546.6 0 241 275 188 546.6 0 188 276 348 546.6 0 348 277 233 546.6 0 233 278 286 546.6 0 286 279 190 546.6 0 190 280 199 546.6 0 199 281 192 546.6 0 192 282 170 546.6 0 170 283 213 546.6 0 213 284 221 546.6 0 221 285 278 546.6 0 278 286 280 546.6 0 280 287 239 546.6 0 239 288 209 546.6 0 209 289 216 546.6 0 216 290 237 546.6 0 237 291 217 546.6 0 217 292 278 546.6 0 278 293 197 546.6 0 197 294 184 546.6 0 184 295 210 546.6 0 210 296 220 546.6 0 220 297 765 546.6 0 765 298 345 546.6 0 345 299 599 546.6 0 599 300 221 546.6 0 221 301 244 546.6 0 244 Finished in 4.38 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 98,375 Reads with adapters: 8,198 (8.3%) Reads that were too short: 132 (0.1%) Reads written (passing filters): 8,066 (8.2%) Total basepairs processed: 22,965,656 bp Total written (filtered): 2,163,478 bp (9.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8198 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 5 0.0 3 0 0 0 1 4 19 75 0.0 3 0 40 15 14 6 20 7551 0.0 4 6920 470 115 34 12 21 33 0.0 4 13 16 2 1 1 22 1 0.0 4 0 0 1 23 39 0.0 4 0 0 0 26 13 30 2 0.0 4 0 0 0 0 2 38 1 0.0 4 1 39 1 0.0 4 0 0 1 40 1 0.0 4 1 54 15 0.0 4 0 0 0 0 15 59 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 70 2 0.0 4 0 0 0 0 2 72 3 0.0 4 0 0 0 2 1 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 80 2 0.0 4 0 0 0 0 2 81 3 0.0 4 0 0 0 0 3 82 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 14 0.0 4 0 0 0 0 14 100 8 0.0 4 0 0 0 0 8 101 2 0.0 4 0 0 0 0 2 103 2 0.0 4 0 0 0 0 2 105 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 113 7 0.0 4 0 0 0 0 7 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 277 0.0 4 0 0 0 0 277 128 2 0.0 4 0 0 0 0 2 129 2 0.0 4 0 0 0 0 2 133 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 141 2 0.0 4 0 0 0 0 2 145 2 0.0 4 1 0 0 0 1 146 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 2 0.0 4 1 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 2 0.0 4 1 1 153 3 0.0 4 0 0 0 0 3 154 4 0.0 4 1 1 0 1 1 155 4 0.0 4 3 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 1 159 10 0.0 4 0 0 0 0 10 160 5 0.0 4 0 0 0 0 5 161 2 0.0 4 0 1 0 0 1 162 2 0.0 4 1 0 0 0 1 164 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 1 1 171 1 0.0 4 0 0 0 1 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 181 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 187 2 0.0 4 0 0 0 0 2 193 1 0.0 4 0 0 0 0 1 197 3 0.0 4 0 0 0 0 3 198 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 210 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 0 2 220 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 4 0.0 4 0 0 0 0 4 229 3 0.0 4 0 0 0 0 3 231 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 1 270 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 280 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 8,066 Reads with adapters: 1,601 (19.8%) Reads written (passing filters): 8,066 (100.0%) Total basepairs processed: 2,163,478 bp Total written (filtered): 2,145,046 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1601 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 9.1% G: 84.1% T: 3.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 18 126.0 0 18 4 3 31.5 0 3 9 9 0.0 0 0 9 10 486 0.0 1 41 445 11 1064 0.0 1 39 1025 12 10 0.0 1 1 9 14 1 0.0 1 0 1 73 1 0.0 1 1 151 6 0.0 1 4 2 190 1 0.0 1 1 209 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.