This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.60 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 76,098 (3.4%) Reads that were too short: 31,634 (1.4%) Reads written (passing filters): 44,464 (2.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 10,296,874 bp (1.5%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 76098 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 271 546.6 0 271 7 8134 546.6 0 8134 8 11177 546.6 0 11177 9 57 546.6 0 57 10 45 546.6 0 45 11 52 546.6 0 52 12 27 546.6 0 27 13 39 546.6 0 39 14 21 546.6 0 21 15 28 546.6 0 28 16 24 546.6 0 24 17 31 546.6 0 31 18 32 546.6 0 32 19 31 546.6 0 31 20 21 546.6 0 21 21 46 546.6 0 46 22 56 546.6 0 56 23 69 546.6 0 69 24 47 546.6 0 47 25 31 546.6 0 31 26 27 546.6 0 27 27 30 546.6 0 30 28 34 546.6 0 34 29 24 546.6 0 24 30 36 546.6 0 36 31 33 546.6 0 33 32 46 546.6 0 46 33 63 546.6 0 63 34 34 546.6 0 34 35 37 546.6 0 37 36 42 546.6 0 42 37 38 546.6 0 38 38 62 546.6 0 62 39 46 546.6 0 46 40 38 546.6 0 38 41 31 546.6 0 31 42 30 546.6 0 30 43 39 546.6 0 39 44 66 546.6 0 66 45 42 546.6 0 42 46 47 546.6 0 47 47 55 546.6 0 55 48 133 546.6 0 133 49 147 546.6 0 147 50 168 546.6 0 168 51 38 546.6 0 38 52 35 546.6 0 35 53 39 546.6 0 39 54 31 546.6 0 31 55 39 546.6 0 39 56 45 546.6 0 45 57 43 546.6 0 43 58 32 546.6 0 32 59 30 546.6 0 30 60 44 546.6 0 44 61 33 546.6 0 33 62 34 546.6 0 34 63 64 546.6 0 64 64 35 546.6 0 35 65 46 546.6 0 46 66 49 546.6 0 49 67 37 546.6 0 37 68 43 546.6 0 43 69 147 546.6 0 147 70 57 546.6 0 57 71 100 546.6 0 100 72 61 546.6 0 61 73 55 546.6 0 55 74 65 546.6 0 65 75 405 546.6 0 405 76 465 546.6 0 465 77 1994 546.6 0 1994 78 1021 546.6 0 1021 79 63 546.6 0 63 80 87 546.6 0 87 81 141 546.6 0 141 82 156 546.6 0 156 83 36 546.6 0 36 84 39 546.6 0 39 85 53 546.6 0 53 86 41 546.6 0 41 87 40 546.6 0 40 88 39 546.6 0 39 89 40 546.6 0 40 90 43 546.6 0 43 91 47 546.6 0 47 92 52 546.6 0 52 93 26 546.6 0 26 94 34 546.6 0 34 95 50 546.6 0 50 96 38 546.6 0 38 97 43 546.6 0 43 98 48 546.6 0 48 99 49 546.6 0 49 100 52 546.6 0 52 101 53 546.6 0 53 102 59 546.6 0 59 103 62 546.6 0 62 104 66 546.6 0 66 105 59 546.6 0 59 106 44 546.6 0 44 107 76 546.6 0 76 108 46 546.6 0 46 109 43 546.6 0 43 110 55 546.6 0 55 111 61 546.6 0 61 112 81 546.6 0 81 113 60 546.6 0 60 114 45 546.6 0 45 115 60 546.6 0 60 116 50 546.6 0 50 117 60 546.6 0 60 118 83 546.6 0 83 119 84 546.6 0 84 120 81 546.6 0 81 121 67 546.6 0 67 122 85 546.6 0 85 123 97 546.6 0 97 124 59 546.6 0 59 125 59 546.6 0 59 126 78 546.6 0 78 127 70 546.6 0 70 128 62 546.6 0 62 129 84 546.6 0 84 130 535 546.6 0 535 131 1502 546.6 0 1502 132 1964 546.6 0 1964 133 94 546.6 0 94 134 104 546.6 0 104 135 94 546.6 0 94 136 731 546.6 0 731 137 1868 546.6 0 1868 138 1757 546.6 0 1757 139 773 546.6 0 773 140 52 546.6 0 52 141 43 546.6 0 43 142 60 546.6 0 60 143 45 546.6 0 45 144 34 546.6 0 34 145 41 546.6 0 41 146 44 546.6 0 44 147 73 546.6 0 73 148 131 546.6 0 131 149 57 546.6 0 57 150 36 546.6 0 36 151 35 546.6 0 35 152 30 546.6 0 30 153 67 546.6 0 67 154 31 546.6 0 31 155 41 546.6 0 41 156 43 546.6 0 43 157 46 546.6 0 46 158 41 546.6 0 41 159 38 546.6 0 38 160 47 546.6 0 47 161 116 546.6 0 116 162 120 546.6 0 120 163 97 546.6 0 97 164 115 546.6 0 115 165 131 546.6 0 131 166 58 546.6 0 58 167 90 546.6 0 90 168 66 546.6 0 66 169 62 546.6 0 62 170 169 546.6 0 169 171 1442 546.6 0 1442 172 71 546.6 0 71 173 50 546.6 0 50 174 36 546.6 0 36 175 46 546.6 0 46 176 46 546.6 0 46 177 45 546.6 0 45 178 22 546.6 0 22 179 26 546.6 0 26 180 31 546.6 0 31 181 34 546.6 0 34 182 32 546.6 0 32 183 44 546.6 0 44 184 61 546.6 0 61 185 47 546.6 0 47 186 45 546.6 0 45 187 79 546.6 0 79 188 59 546.6 0 59 189 377 546.6 0 377 190 150 546.6 0 150 191 62 546.6 0 62 192 29 546.6 0 29 193 35 546.6 0 35 194 31 546.6 0 31 195 52 546.6 0 52 196 70 546.6 0 70 197 38 546.6 0 38 198 34 546.6 0 34 199 30 546.6 0 30 200 31 546.6 0 31 201 48 546.6 0 48 202 45 546.6 0 45 203 36 546.6 0 36 204 35 546.6 0 35 205 36 546.6 0 36 206 23 546.6 0 23 207 39 546.6 0 39 208 39 546.6 0 39 209 54 546.6 0 54 210 32 546.6 0 32 211 42 546.6 0 42 212 33 546.6 0 33 213 44 546.6 0 44 214 34 546.6 0 34 215 29 546.6 0 29 216 35 546.6 0 35 217 35 546.6 0 35 218 38 546.6 0 38 219 43 546.6 0 43 220 47 546.6 0 47 221 49 546.6 0 49 222 166 546.6 0 166 223 133 546.6 0 133 224 162 546.6 0 162 225 60 546.6 0 60 226 152 546.6 0 152 227 259 546.6 0 259 228 291 546.6 0 291 229 360 546.6 0 360 230 311 546.6 0 311 231 256 546.6 0 256 232 266 546.6 0 266 233 173 546.6 0 173 234 68 546.6 0 68 235 86 546.6 0 86 236 99 546.6 0 99 237 95 546.6 0 95 238 153 546.6 0 153 239 109 546.6 0 109 240 282 546.6 0 282 241 187 546.6 0 187 242 172 546.6 0 172 243 177 546.6 0 177 244 235 546.6 0 235 245 172 546.6 0 172 246 326 546.6 0 326 247 346 546.6 0 346 248 400 546.6 0 400 249 218 546.6 0 218 250 463 546.6 0 463 251 2877 546.6 0 2877 252 4089 546.6 0 4089 253 11791 546.6 0 11791 254 394 546.6 0 394 255 85 546.6 0 85 256 93 546.6 0 93 257 88 546.6 0 88 258 231 546.6 0 231 259 80 546.6 0 80 260 175 546.6 0 175 261 170 546.6 0 170 262 102 546.6 0 102 263 91 546.6 0 91 264 106 546.6 0 106 265 84 546.6 0 84 266 77 546.6 0 77 267 74 546.6 0 74 268 66 546.6 0 66 269 73 546.6 0 73 270 76 546.6 0 76 271 90 546.6 0 90 272 89 546.6 0 89 273 78 546.6 0 78 274 53 546.6 0 53 275 54 546.6 0 54 276 70 546.6 0 70 277 95 546.6 0 95 278 71 546.6 0 71 279 56 546.6 0 56 280 56 546.6 0 56 281 74 546.6 0 74 282 68 546.6 0 68 283 91 546.6 0 91 284 114 546.6 0 114 285 168 546.6 0 168 286 190 546.6 0 190 287 138 546.6 0 138 288 98 546.6 0 98 289 125 546.6 0 125 290 128 546.6 0 128 291 120 546.6 0 120 292 125 546.6 0 125 293 94 546.6 0 94 294 100 546.6 0 100 295 156 546.6 0 156 296 103 546.6 0 103 297 536 546.6 0 536 298 189 546.6 0 189 299 324 546.6 0 324 300 86 546.6 0 86 301 128 546.6 0 128 Finished in 1.86 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 44,464 Reads with adapters: 7,951 (17.9%) Reads that were too short: 28 (0.1%) Reads written (passing filters): 7,923 (17.8%) Total basepairs processed: 10,296,874 bp Total written (filtered): 2,185,075 bp (21.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7951 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 57 0.0 3 0 53 3 1 17 7781 0.0 3 7599 172 9 1 18 17 0.0 3 2 14 1 19 7 0.0 3 1 0 6 20 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 3 0.0 3 0 0 0 3 36 1 0.0 3 1 51 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 31 0.0 3 0 0 1 30 64 6 0.0 3 0 0 0 6 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 97 4 0.0 3 0 0 0 4 100 3 0.0 3 0 0 0 3 110 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 1 150 1 0.0 3 0 0 1 151 4 0.0 3 1 1 1 1 152 1 0.0 3 0 0 1 153 2 0.0 3 0 1 0 1 154 2 0.0 3 0 0 2 162 1 0.0 3 0 0 0 1 171 4 0.0 3 0 0 0 4 175 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 7,923 Reads with adapters: 36 (0.5%) Reads written (passing filters): 7,923 (100.0%) Total basepairs processed: 2,185,075 bp Total written (filtered): 2,184,964 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 36 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 75.0% G: 2.8% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 34 123.8 0 34 4 1 30.9 0 1 5 1 7.7 0 1