This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.64 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 426,683 (19.1%) Reads that were too short: 360,971 (16.1%) Reads written (passing filters): 65,712 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,660,104 bp (2.6%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 426683 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1460 546.6 0 1460 7 29441 546.6 0 29441 8 425 546.6 0 425 9 23251 546.6 0 23251 10 43 546.6 0 43 11 32 546.6 0 32 12 16 546.6 0 16 13 37 546.6 0 37 14 15 546.6 0 15 15 21 546.6 0 21 16 7 546.6 0 7 17 11 546.6 0 11 18 25 546.6 0 25 19 18 546.6 0 18 20 27 546.6 0 27 21 34 546.6 0 34 22 21 546.6 0 21 23 17 546.6 0 17 24 12 546.6 0 12 25 16 546.6 0 16 26 9 546.6 0 9 27 16 546.6 0 16 28 16 546.6 0 16 29 15 546.6 0 15 30 14 546.6 0 14 31 13 546.6 0 13 32 14 546.6 0 14 33 20 546.6 0 20 34 16 546.6 0 16 35 22 546.6 0 22 36 23 546.6 0 23 37 15 546.6 0 15 38 25 546.6 0 25 39 15 546.6 0 15 40 13 546.6 0 13 41 34 546.6 0 34 42 19 546.6 0 19 43 26 546.6 0 26 44 13 546.6 0 13 45 20 546.6 0 20 46 25 546.6 0 25 47 28 546.6 0 28 48 10 546.6 0 10 49 43 546.6 0 43 50 21 546.6 0 21 51 23 546.6 0 23 52 14 546.6 0 14 53 46 546.6 0 46 54 44 546.6 0 44 55 68 546.6 0 68 56 36 546.6 0 36 57 17 546.6 0 17 58 15 546.6 0 15 59 22 546.6 0 22 60 19 546.6 0 19 61 18 546.6 0 18 62 27 546.6 0 27 63 35 546.6 0 35 64 11 546.6 0 11 65 20 546.6 0 20 66 17 546.6 0 17 67 13 546.6 0 13 68 12 546.6 0 12 69 17 546.6 0 17 70 18 546.6 0 18 71 22 546.6 0 22 72 37 546.6 0 37 73 31 546.6 0 31 74 37 546.6 0 37 75 18 546.6 0 18 76 13 546.6 0 13 77 12 546.6 0 12 78 19 546.6 0 19 79 7 546.6 0 7 80 20 546.6 0 20 81 27 546.6 0 27 82 16 546.6 0 16 83 20 546.6 0 20 84 20 546.6 0 20 85 41 546.6 0 41 86 27 546.6 0 27 87 23 546.6 0 23 88 23 546.6 0 23 89 18 546.6 0 18 90 18 546.6 0 18 91 13 546.6 0 13 92 14 546.6 0 14 93 25 546.6 0 25 94 9 546.6 0 9 95 20 546.6 0 20 96 17 546.6 0 17 97 13 546.6 0 13 98 25 546.6 0 25 99 30 546.6 0 30 100 17 546.6 0 17 101 8 546.6 0 8 102 13 546.6 0 13 103 13 546.6 0 13 104 18 546.6 0 18 105 24 546.6 0 24 106 17 546.6 0 17 107 23 546.6 0 23 108 32 546.6 0 32 109 16 546.6 0 16 110 6 546.6 0 6 111 18 546.6 0 18 112 23 546.6 0 23 113 36 546.6 0 36 114 20 546.6 0 20 115 42 546.6 0 42 116 60 546.6 0 60 117 30 546.6 0 30 118 25 546.6 0 25 119 16 546.6 0 16 120 26 546.6 0 26 121 11 546.6 0 11 122 44 546.6 0 44 123 52 546.6 0 52 124 46 546.6 0 46 125 315 546.6 0 315 126 54 546.6 0 54 127 40 546.6 0 40 128 42 546.6 0 42 129 40 546.6 0 40 130 29 546.6 0 29 131 13 546.6 0 13 132 17 546.6 0 17 133 21 546.6 0 21 134 26 546.6 0 26 135 35 546.6 0 35 136 20 546.6 0 20 137 27 546.6 0 27 138 23 546.6 0 23 139 14 546.6 0 14 140 29 546.6 0 29 141 21 546.6 0 21 142 41 546.6 0 41 143 26 546.6 0 26 144 26 546.6 0 26 145 18 546.6 0 18 146 36 546.6 0 36 147 23 546.6 0 23 148 29 546.6 0 29 149 52 546.6 0 52 150 59 546.6 0 59 151 89 546.6 0 89 152 131 546.6 0 131 153 37 546.6 0 37 154 33 546.6 0 33 155 40 546.6 0 40 156 38 546.6 0 38 157 20 546.6 0 20 158 27 546.6 0 27 159 33 546.6 0 33 160 52 546.6 0 52 161 49 546.6 0 49 162 96 546.6 0 96 163 35 546.6 0 35 164 36 546.6 0 36 165 132 546.6 0 132 166 127 546.6 0 127 167 176 546.6 0 176 168 78 546.6 0 78 169 85 546.6 0 85 170 74 546.6 0 74 171 50 546.6 0 50 172 51 546.6 0 51 173 32 546.6 0 32 174 27 546.6 0 27 175 34 546.6 0 34 176 42 546.6 0 42 177 56 546.6 0 56 178 50 546.6 0 50 179 81 546.6 0 81 180 60 546.6 0 60 181 66 546.6 0 66 182 60 546.6 0 60 183 69 546.6 0 69 184 58 546.6 0 58 185 457 546.6 0 457 186 276 546.6 0 276 187 1595 546.6 0 1595 188 1440 546.6 0 1440 189 645 546.6 0 645 190 30 546.6 0 30 191 38 546.6 0 38 192 44 546.6 0 44 193 50 546.6 0 50 194 46 546.6 0 46 195 34 546.6 0 34 196 34 546.6 0 34 197 49 546.6 0 49 198 52 546.6 0 52 199 100 546.6 0 100 200 383 546.6 0 383 201 82 546.6 0 82 202 59 546.6 0 59 203 72 546.6 0 72 204 75 546.6 0 75 205 34 546.6 0 34 206 83 546.6 0 83 207 140 546.6 0 140 208 48 546.6 0 48 209 81 546.6 0 81 210 532 546.6 0 532 211 46 546.6 0 46 212 28 546.6 0 28 213 42 546.6 0 42 214 51 546.6 0 51 215 41 546.6 0 41 216 43 546.6 0 43 217 110 546.6 0 110 218 192 546.6 0 192 219 332 546.6 0 332 220 1061 546.6 0 1061 221 1604 546.6 0 1604 222 3581 546.6 0 3581 223 1853 546.6 0 1853 224 1229 546.6 0 1229 225 437 546.6 0 437 226 218 546.6 0 218 227 315 546.6 0 315 228 150 546.6 0 150 229 249 546.6 0 249 230 397 546.6 0 397 231 381 546.6 0 381 232 697 546.6 0 697 233 1298 546.6 0 1298 234 1653 546.6 0 1653 235 3882 546.6 0 3882 236 8461 546.6 0 8461 237 147741 546.6 0 147741 238 99254 546.6 0 99254 239 71388 546.6 0 71388 240 2495 546.6 0 2495 241 961 546.6 0 961 242 114 546.6 0 114 243 76 546.6 0 76 244 74 546.6 0 74 245 1092 546.6 0 1092 246 453 546.6 0 453 247 2764 546.6 0 2764 248 933 546.6 0 933 249 510 546.6 0 510 250 232 546.6 0 232 251 57 546.6 0 57 252 45 546.6 0 45 253 12 546.6 0 12 254 16 546.6 0 16 255 25 546.6 0 25 256 25 546.6 0 25 257 26 546.6 0 26 258 20 546.6 0 20 259 12 546.6 0 12 260 38 546.6 0 38 261 28 546.6 0 28 262 39 546.6 0 39 263 26 546.6 0 26 264 25 546.6 0 25 265 23 546.6 0 23 266 31 546.6 0 31 267 83 546.6 0 83 268 173 546.6 0 173 269 244 546.6 0 244 270 172 546.6 0 172 271 61 546.6 0 61 272 37 546.6 0 37 273 40 546.6 0 40 274 65 546.6 0 65 275 35 546.6 0 35 276 41 546.6 0 41 277 37 546.6 0 37 278 28 546.6 0 28 279 26 546.6 0 26 280 37 546.6 0 37 281 57 546.6 0 57 282 87 546.6 0 87 283 103 546.6 0 103 284 51 546.6 0 51 285 36 546.6 0 36 286 51 546.6 0 51 287 139 546.6 0 139 288 152 546.6 0 152 289 195 546.6 0 195 290 136 546.6 0 136 291 135 546.6 0 135 292 44 546.6 0 44 293 25 546.6 0 25 294 116 546.6 0 116 295 144 546.6 0 144 296 149 546.6 0 149 297 74 546.6 0 74 298 53 546.6 0 53 299 58 546.6 0 58 300 37 546.6 0 37 301 40 546.6 0 40 Finished in 3.02 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 65,712 Reads with adapters: 23,978 (36.5%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 23,945 (36.4%) Total basepairs processed: 17,660,104 bp Total written (filtered): 6,581,070 bp (37.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23978 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 102 0.0 3 0 101 1 17 23718 0.0 3 23167 527 22 2 18 47 0.0 3 3 43 1 19 22 0.0 3 0 0 19 3 20 20 0.0 3 3 0 0 17 21 6 0.0 3 0 0 0 6 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 32 24 0.0 3 0 0 0 24 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 0 3 121 3 0.0 3 0 0 1 2 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 1 231 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 246 3 0.0 3 0 0 0 3 279 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 23,945 Reads with adapters: 853 (3.6%) Reads written (passing filters): 23,945 (100.0%) Total basepairs processed: 6,581,070 bp Total written (filtered): 6,578,419 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 853 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.7% C: 6.8% G: 18.9% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 764 374.1 0 764 4 87 93.5 0 87 5 1 23.4 0 1 6 1 5.8 0 1