This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.30 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 273,826 (12.2%) Reads that were too short: 5,597 (0.2%) Reads written (passing filters): 268,229 (12.0%) Total basepairs processed: 673,916,425 bp Total written (filtered): 42,393,087 bp (6.3%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 273826 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 105 546.6 0 105 7 6130 546.6 0 6130 8 366 546.6 0 366 9 5901 546.6 0 5901 10 45 546.6 0 45 11 28 546.6 0 28 12 24 546.6 0 24 13 24 546.6 0 24 14 32 546.6 0 32 15 52 546.6 0 52 16 23 546.6 0 23 17 15 546.6 0 15 18 21 546.6 0 21 19 34 546.6 0 34 20 38 546.6 0 38 21 27 546.6 0 27 22 20 546.6 0 20 23 36 546.6 0 36 24 39 546.6 0 39 25 37 546.6 0 37 26 36 546.6 0 36 27 154 546.6 0 154 28 108 546.6 0 108 29 99 546.6 0 99 30 27 546.6 0 27 31 33 546.6 0 33 32 27 546.6 0 27 33 23 546.6 0 23 34 38 546.6 0 38 35 33 546.6 0 33 36 25 546.6 0 25 37 54 546.6 0 54 38 37 546.6 0 37 39 26 546.6 0 26 40 31 546.6 0 31 41 37 546.6 0 37 42 35 546.6 0 35 43 40 546.6 0 40 44 41 546.6 0 41 45 36 546.6 0 36 46 37 546.6 0 37 47 64 546.6 0 64 48 29 546.6 0 29 49 49 546.6 0 49 50 64 546.6 0 64 51 57 546.6 0 57 52 37 546.6 0 37 53 36 546.6 0 36 54 33 546.6 0 33 55 24 546.6 0 24 56 35 546.6 0 35 57 75 546.6 0 75 58 97 546.6 0 97 59 198 546.6 0 198 60 51 546.6 0 51 61 37 546.6 0 37 62 31 546.6 0 31 63 39 546.6 0 39 64 20 546.6 0 20 65 33 546.6 0 33 66 24 546.6 0 24 67 43 546.6 0 43 68 31 546.6 0 31 69 30 546.6 0 30 70 41 546.6 0 41 71 39 546.6 0 39 72 27 546.6 0 27 73 37 546.6 0 37 74 29 546.6 0 29 75 38 546.6 0 38 76 19 546.6 0 19 77 40 546.6 0 40 78 44 546.6 0 44 79 27 546.6 0 27 80 30 546.6 0 30 81 94 546.6 0 94 82 39 546.6 0 39 83 33 546.6 0 33 84 54 546.6 0 54 85 62 546.6 0 62 86 46 546.6 0 46 87 35 546.6 0 35 88 66 546.6 0 66 89 73 546.6 0 73 90 70 546.6 0 70 91 157 546.6 0 157 92 90 546.6 0 90 93 89 546.6 0 89 94 108 546.6 0 108 95 89 546.6 0 89 96 110 546.6 0 110 97 50 546.6 0 50 98 58 546.6 0 58 99 55 546.6 0 55 100 41 546.6 0 41 101 89 546.6 0 89 102 66 546.6 0 66 103 109 546.6 0 109 104 216 546.6 0 216 105 275 546.6 0 275 106 488 546.6 0 488 107 1229 546.6 0 1229 108 20701 546.6 0 20701 109 5673 546.6 0 5673 110 13224 546.6 0 13224 111 146 546.6 0 146 112 39 546.6 0 39 113 42 546.6 0 42 114 36 546.6 0 36 115 63 546.6 0 63 116 108 546.6 0 108 117 79 546.6 0 79 118 89 546.6 0 89 119 63 546.6 0 63 120 52 546.6 0 52 121 59 546.6 0 59 122 42 546.6 0 42 123 56 546.6 0 56 124 58 546.6 0 58 125 107 546.6 0 107 126 62 546.6 0 62 127 90 546.6 0 90 128 128 546.6 0 128 129 89 546.6 0 89 130 95 546.6 0 95 131 99 546.6 0 99 132 65 546.6 0 65 133 108 546.6 0 108 134 1031 546.6 0 1031 135 1237 546.6 0 1237 136 2183 546.6 0 2183 137 105 546.6 0 105 138 81 546.6 0 81 139 56 546.6 0 56 140 120 546.6 0 120 141 72 546.6 0 72 142 165 546.6 0 165 143 143 546.6 0 143 144 187 546.6 0 187 145 336 546.6 0 336 146 636 546.6 0 636 147 1300 546.6 0 1300 148 384 546.6 0 384 149 420 546.6 0 420 150 789 546.6 0 789 151 391 546.6 0 391 152 339 546.6 0 339 153 764 546.6 0 764 154 751 546.6 0 751 155 2950 546.6 0 2950 156 2382 546.6 0 2382 157 2948 546.6 0 2948 158 1699 546.6 0 1699 159 2276 546.6 0 2276 160 77278 546.6 0 77278 161 19502 546.6 0 19502 162 85047 546.6 0 85047 163 300 546.6 0 300 164 56 546.6 0 56 165 49 546.6 0 49 166 46 546.6 0 46 167 46 546.6 0 46 168 28 546.6 0 28 169 57 546.6 0 57 170 36 546.6 0 36 171 59 546.6 0 59 172 38 546.6 0 38 173 28 546.6 0 28 174 57 546.6 0 57 175 64 546.6 0 64 176 27 546.6 0 27 177 38 546.6 0 38 178 34 546.6 0 34 179 49 546.6 0 49 180 58 546.6 0 58 181 38 546.6 0 38 182 39 546.6 0 39 183 45 546.6 0 45 184 37 546.6 0 37 185 23 546.6 0 23 186 34 546.6 0 34 187 44 546.6 0 44 188 28 546.6 0 28 189 34 546.6 0 34 190 46 546.6 0 46 191 82 546.6 0 82 192 61 546.6 0 61 193 77 546.6 0 77 194 36 546.6 0 36 195 32 546.6 0 32 196 29 546.6 0 29 197 26 546.6 0 26 198 42 546.6 0 42 199 28 546.6 0 28 200 25 546.6 0 25 201 35 546.6 0 35 202 51 546.6 0 51 203 77 546.6 0 77 204 39 546.6 0 39 205 32 546.6 0 32 206 38 546.6 0 38 207 32 546.6 0 32 208 36 546.6 0 36 209 36 546.6 0 36 210 34 546.6 0 34 211 19 546.6 0 19 212 21 546.6 0 21 213 9 546.6 0 9 214 29 546.6 0 29 215 30 546.6 0 30 216 35 546.6 0 35 217 43 546.6 0 43 218 53 546.6 0 53 219 54 546.6 0 54 220 49 546.6 0 49 221 48 546.6 0 48 222 150 546.6 0 150 223 95 546.6 0 95 224 137 546.6 0 137 225 57 546.6 0 57 226 35 546.6 0 35 227 49 546.6 0 49 228 23 546.6 0 23 229 39 546.6 0 39 230 42 546.6 0 42 231 44 546.6 0 44 232 46 546.6 0 46 233 54 546.6 0 54 234 108 546.6 0 108 235 65 546.6 0 65 236 86 546.6 0 86 237 38 546.6 0 38 238 32 546.6 0 32 239 25 546.6 0 25 240 29 546.6 0 29 241 45 546.6 0 45 242 46 546.6 0 46 243 37 546.6 0 37 244 39 546.6 0 39 245 25 546.6 0 25 246 26 546.6 0 26 247 37 546.6 0 37 248 40 546.6 0 40 249 43 546.6 0 43 250 68 546.6 0 68 251 276 546.6 0 276 252 220 546.6 0 220 253 191 546.6 0 191 254 226 546.6 0 226 255 84 546.6 0 84 256 70 546.6 0 70 257 37 546.6 0 37 258 43 546.6 0 43 259 42 546.6 0 42 260 36 546.6 0 36 261 90 546.6 0 90 262 91 546.6 0 91 263 93 546.6 0 93 264 30 546.6 0 30 265 42 546.6 0 42 266 29 546.6 0 29 267 36 546.6 0 36 268 28 546.6 0 28 269 37 546.6 0 37 270 43 546.6 0 43 271 30 546.6 0 30 272 170 546.6 0 170 273 36 546.6 0 36 274 25 546.6 0 25 275 41 546.6 0 41 276 27 546.6 0 27 277 33 546.6 0 33 278 98 546.6 0 98 279 67 546.6 0 67 280 61 546.6 0 61 281 92 546.6 0 92 282 89 546.6 0 89 283 99 546.6 0 99 284 62 546.6 0 62 285 39 546.6 0 39 286 25 546.6 0 25 287 35 546.6 0 35 288 18 546.6 0 18 289 30 546.6 0 30 290 43 546.6 0 43 291 56 546.6 0 56 292 42 546.6 0 42 293 34 546.6 0 34 294 23 546.6 0 23 295 31 546.6 0 31 296 32 546.6 0 32 297 40 546.6 0 40 298 39 546.6 0 39 299 30 546.6 0 30 300 20 546.6 0 20 301 61 546.6 0 61 Finished in 8.11 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 268,229 Reads with adapters: 7,906 (2.9%) Reads that were too short: 101 (0.0%) Reads written (passing filters): 7,805 (2.9%) Total basepairs processed: 42,393,087 bp Total written (filtered): 1,865,699 bp (4.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7906 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 20 0.0 3 0 18 1 1 17 5990 0.0 3 5880 104 5 1 18 23 0.0 3 11 12 19 3 0.0 3 0 0 2 1 20 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 31 1608 0.0 3 0 0 11 1597 34 131 0.0 3 0 0 2 129 36 1 0.0 3 0 0 0 1 37 1 0.0 3 1 44 1 0.0 3 0 0 0 1 49 1 0.0 3 1 57 10 0.0 3 0 0 0 10 58 1 0.0 3 0 0 0 1 62 1 0.0 3 1 63 5 0.0 3 0 0 0 5 82 11 0.0 3 0 0 0 11 83 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 100 1 0.0 3 1 101 3 0.0 3 2 0 0 1 110 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 132 52 0.0 3 0 0 2 50 133 6 0.0 3 0 0 1 5 134 3 0.0 3 0 0 0 3 135 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 159 5 0.0 3 0 0 2 3 177 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 7,805 Reads with adapters: 33 (0.4%) Reads written (passing filters): 7,805 (100.0%) Total basepairs processed: 1,865,699 bp Total written (filtered): 1,865,590 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 33 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 27.3% G: 33.3% T: 6.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 29 122.0 0 29 4 2 30.5 0 2 5 1 7.6 0 1 9 1 0.0 0 0 1