This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.75 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 304,148 (13.6%) Reads that were too short: 8,944 (0.4%) Reads written (passing filters): 295,204 (13.2%) Total basepairs processed: 673,916,425 bp Total written (filtered): 54,393,045 bp (8.1%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 304148 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 118 546.6 0 118 7 52 546.6 0 52 8 46 546.6 0 46 9 76 546.6 0 76 10 43 546.6 0 43 11 46 546.6 0 46 12 44 546.6 0 44 13 48 546.6 0 48 14 58 546.6 0 58 15 66 546.6 0 66 16 49 546.6 0 49 17 71 546.6 0 71 18 51 546.6 0 51 19 58 546.6 0 58 20 36 546.6 0 36 21 38 546.6 0 38 22 39 546.6 0 39 23 62 546.6 0 62 24 79 546.6 0 79 25 164 546.6 0 164 26 126 546.6 0 126 27 177 546.6 0 177 28 50 546.6 0 50 29 59 546.6 0 59 30 57 546.6 0 57 31 48 546.6 0 48 32 52 546.6 0 52 33 51 546.6 0 51 34 36 546.6 0 36 35 58 546.6 0 58 36 65 546.6 0 65 37 75 546.6 0 75 38 82 546.6 0 82 39 57 546.6 0 57 40 48 546.6 0 48 41 44 546.6 0 44 42 54 546.6 0 54 43 58 546.6 0 58 44 64 546.6 0 64 45 48 546.6 0 48 46 47 546.6 0 47 47 50 546.6 0 50 48 51 546.6 0 51 49 31 546.6 0 31 50 39 546.6 0 39 51 56 546.6 0 56 52 59 546.6 0 59 53 45 546.6 0 45 54 45 546.6 0 45 55 95 546.6 0 95 56 73 546.6 0 73 57 41 546.6 0 41 58 61 546.6 0 61 59 36 546.6 0 36 60 55 546.6 0 55 61 73 546.6 0 73 62 43 546.6 0 43 63 50 546.6 0 50 64 51 546.6 0 51 65 49 546.6 0 49 66 83 546.6 0 83 67 79 546.6 0 79 68 89 546.6 0 89 69 137 546.6 0 137 70 1451 546.6 0 1451 71 433 546.6 0 433 72 691 546.6 0 691 73 49 546.6 0 49 74 66 546.6 0 66 75 42 546.6 0 42 76 94 546.6 0 94 77 94 546.6 0 94 78 73 546.6 0 73 79 101 546.6 0 101 80 111 546.6 0 111 81 67 546.6 0 67 82 137 546.6 0 137 83 144 546.6 0 144 84 222 546.6 0 222 85 102 546.6 0 102 86 397 546.6 0 397 87 69 546.6 0 69 88 53 546.6 0 53 89 66 546.6 0 66 90 54 546.6 0 54 91 57 546.6 0 57 92 73 546.6 0 73 93 88 546.6 0 88 94 74 546.6 0 74 95 212 546.6 0 212 96 479 546.6 0 479 97 138 546.6 0 138 98 249 546.6 0 249 99 119 546.6 0 119 100 174 546.6 0 174 101 266 546.6 0 266 102 139 546.6 0 139 103 143 546.6 0 143 104 104 546.6 0 104 105 162 546.6 0 162 106 291 546.6 0 291 107 350 546.6 0 350 108 622 546.6 0 622 109 887 546.6 0 887 110 1282 546.6 0 1282 111 2984 546.6 0 2984 112 8991 546.6 0 8991 113 125132 546.6 0 125132 114 32886 546.6 0 32886 115 52984 546.6 0 52984 116 255 546.6 0 255 117 77 546.6 0 77 118 81 546.6 0 81 119 64 546.6 0 64 120 65 546.6 0 65 121 109 546.6 0 109 122 231 546.6 0 231 123 77 546.6 0 77 124 98 546.6 0 98 125 159 546.6 0 159 126 103 546.6 0 103 127 268 546.6 0 268 128 236 546.6 0 236 129 714 546.6 0 714 130 246 546.6 0 246 131 222 546.6 0 222 132 386 546.6 0 386 133 580 546.6 0 580 134 473 546.6 0 473 135 2471 546.6 0 2471 136 15480 546.6 0 15480 137 28533 546.6 0 28533 138 212 546.6 0 212 139 317 546.6 0 317 140 329 546.6 0 329 141 334 546.6 0 334 142 151 546.6 0 151 143 124 546.6 0 124 144 86 546.6 0 86 145 235 546.6 0 235 146 64 546.6 0 64 147 129 546.6 0 129 148 51 546.6 0 51 149 95 546.6 0 95 150 73 546.6 0 73 151 50 546.6 0 50 152 49 546.6 0 49 153 63 546.6 0 63 154 66 546.6 0 66 155 64 546.6 0 64 156 76 546.6 0 76 157 74 546.6 0 74 158 144 546.6 0 144 159 702 546.6 0 702 160 446 546.6 0 446 161 122 546.6 0 122 162 45 546.6 0 45 163 55 546.6 0 55 164 68 546.6 0 68 165 65 546.6 0 65 166 71 546.6 0 71 167 67 546.6 0 67 168 92 546.6 0 92 169 41 546.6 0 41 170 58 546.6 0 58 171 48 546.6 0 48 172 50 546.6 0 50 173 43 546.6 0 43 174 48 546.6 0 48 175 70 546.6 0 70 176 77 546.6 0 77 177 72 546.6 0 72 178 64 546.6 0 64 179 147 546.6 0 147 180 279 546.6 0 279 181 161 546.6 0 161 182 89 546.6 0 89 183 51 546.6 0 51 184 87 546.6 0 87 185 85 546.6 0 85 186 80 546.6 0 80 187 60 546.6 0 60 188 75 546.6 0 75 189 41 546.6 0 41 190 43 546.6 0 43 191 54 546.6 0 54 192 34 546.6 0 34 193 51 546.6 0 51 194 53 546.6 0 53 195 43 546.6 0 43 196 63 546.6 0 63 197 49 546.6 0 49 198 64 546.6 0 64 199 68 546.6 0 68 200 44 546.6 0 44 201 72 546.6 0 72 202 86 546.6 0 86 203 141 546.6 0 141 204 92 546.6 0 92 205 103 546.6 0 103 206 171 546.6 0 171 207 64 546.6 0 64 208 86 546.6 0 86 209 51 546.6 0 51 210 73 546.6 0 73 211 72 546.6 0 72 212 70 546.6 0 70 213 61 546.6 0 61 214 88 546.6 0 88 215 128 546.6 0 128 216 109 546.6 0 109 217 58 546.6 0 58 218 41 546.6 0 41 219 76 546.6 0 76 220 111 546.6 0 111 221 138 546.6 0 138 222 58 546.6 0 58 223 86 546.6 0 86 224 444 546.6 0 444 225 159 546.6 0 159 226 706 546.6 0 706 227 57 546.6 0 57 228 54 546.6 0 54 229 43 546.6 0 43 230 53 546.6 0 53 231 49 546.6 0 49 232 49 546.6 0 49 233 63 546.6 0 63 234 189 546.6 0 189 235 139 546.6 0 139 236 166 546.6 0 166 237 67 546.6 0 67 238 49 546.6 0 49 239 49 546.6 0 49 240 37 546.6 0 37 241 63 546.6 0 63 242 55 546.6 0 55 243 51 546.6 0 51 244 60 546.6 0 60 245 65 546.6 0 65 246 50 546.6 0 50 247 62 546.6 0 62 248 49 546.6 0 49 249 58 546.6 0 58 250 44 546.6 0 44 251 88 546.6 0 88 252 40 546.6 0 40 253 57 546.6 0 57 254 53 546.6 0 53 255 59 546.6 0 59 256 72 546.6 0 72 257 80 546.6 0 80 258 73 546.6 0 73 259 57 546.6 0 57 260 59 546.6 0 59 261 76 546.6 0 76 262 55 546.6 0 55 263 50 546.6 0 50 264 57 546.6 0 57 265 50 546.6 0 50 266 56 546.6 0 56 267 51 546.6 0 51 268 68 546.6 0 68 269 48 546.6 0 48 270 85 546.6 0 85 271 147 546.6 0 147 272 122 546.6 0 122 273 264 546.6 0 264 274 127 546.6 0 127 275 121 546.6 0 121 276 170 546.6 0 170 277 115 546.6 0 115 278 47 546.6 0 47 279 44 546.6 0 44 280 98 546.6 0 98 281 102 546.6 0 102 282 185 546.6 0 185 283 51 546.6 0 51 284 56 546.6 0 56 285 47 546.6 0 47 286 49 546.6 0 49 287 67 546.6 0 67 288 43 546.6 0 43 289 49 546.6 0 49 290 36 546.6 0 36 291 54 546.6 0 54 292 60 546.6 0 60 293 97 546.6 0 97 294 110 546.6 0 110 295 184 546.6 0 184 296 131 546.6 0 131 297 90 546.6 0 90 298 44 546.6 0 44 299 41 546.6 0 41 300 29 546.6 0 29 301 37 546.6 0 37 Finished in 10.80 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 295,204 Reads with adapters: 83 (0.0%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 42 (0.0%) Total basepairs processed: 54,393,045 bp Total written (filtered): 10,144 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 83 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 18 0.0 4 6 7 3 0 2 22 12 0.0 4 12 23 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 27 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 1 68 1 0.0 4 0 0 0 0 1 79 4 0.0 4 0 0 0 0 4 84 2 0.0 4 0 0 0 1 1 93 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 1 105 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 122 13 0.0 4 0 0 0 1 12 123 10 0.0 4 0 0 0 0 10 124 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (238 us/read; 0.25 M reads/minute). === Summary === Total reads processed: 42 Reads with adapters: 3 (7.1%) Reads written (passing filters): 42 (100.0%) Total basepairs processed: 10,144 bp Total written (filtered): 10,114 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 1 10 1 0.0 1 0 1 11 1 0.0 1 0 1