This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.95 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 357,619 (16.0%) Reads that were too short: 6,123 (0.3%) Reads written (passing filters): 351,496 (15.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 65,072,842 bp (9.7%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 357619 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 59 546.6 0 59 7 59 546.6 0 59 8 47 546.6 0 47 9 74 546.6 0 74 10 39 546.6 0 39 11 49 546.6 0 49 12 45 546.6 0 45 13 30 546.6 0 30 14 58 546.6 0 58 15 53 546.6 0 53 16 45 546.6 0 45 17 57 546.6 0 57 18 63 546.6 0 63 19 59 546.6 0 59 20 42 546.6 0 42 21 50 546.6 0 50 22 63 546.6 0 63 23 50 546.6 0 50 24 51 546.6 0 51 25 155 546.6 0 155 26 101 546.6 0 101 27 90 546.6 0 90 28 57 546.6 0 57 29 40 546.6 0 40 30 40 546.6 0 40 31 54 546.6 0 54 32 68 546.6 0 68 33 39 546.6 0 39 34 41 546.6 0 41 35 36 546.6 0 36 36 53 546.6 0 53 37 42 546.6 0 42 38 68 546.6 0 68 39 39 546.6 0 39 40 45 546.6 0 45 41 30 546.6 0 30 42 67 546.6 0 67 43 52 546.6 0 52 44 53 546.6 0 53 45 41 546.6 0 41 46 41 546.6 0 41 47 42 546.6 0 42 48 39 546.6 0 39 49 29 546.6 0 29 50 43 546.6 0 43 51 34 546.6 0 34 52 41 546.6 0 41 53 55 546.6 0 55 54 36 546.6 0 36 55 95 546.6 0 95 56 67 546.6 0 67 57 48 546.6 0 48 58 50 546.6 0 50 59 39 546.6 0 39 60 47 546.6 0 47 61 62 546.6 0 62 62 47 546.6 0 47 63 49 546.6 0 49 64 47 546.6 0 47 65 50 546.6 0 50 66 71 546.6 0 71 67 68 546.6 0 68 68 75 546.6 0 75 69 132 546.6 0 132 70 1665 546.6 0 1665 71 478 546.6 0 478 72 870 546.6 0 870 73 64 546.6 0 64 74 74 546.6 0 74 75 48 546.6 0 48 76 107 546.6 0 107 77 81 546.6 0 81 78 83 546.6 0 83 79 87 546.6 0 87 80 80 546.6 0 80 81 76 546.6 0 76 82 113 546.6 0 113 83 129 546.6 0 129 84 220 546.6 0 220 85 105 546.6 0 105 86 456 546.6 0 456 87 55 546.6 0 55 88 62 546.6 0 62 89 52 546.6 0 52 90 42 546.6 0 42 91 71 546.6 0 71 92 71 546.6 0 71 93 106 546.6 0 106 94 75 546.6 0 75 95 210 546.6 0 210 96 578 546.6 0 578 97 148 546.6 0 148 98 234 546.6 0 234 99 118 546.6 0 118 100 202 546.6 0 202 101 373 546.6 0 373 102 153 546.6 0 153 103 162 546.6 0 162 104 101 546.6 0 101 105 156 546.6 0 156 106 304 546.6 0 304 107 319 546.6 0 319 108 641 546.6 0 641 109 1031 546.6 0 1031 110 1418 546.6 0 1418 111 3243 546.6 0 3243 112 8923 546.6 0 8923 113 165357 546.6 0 165357 114 39807 546.6 0 39807 115 65700 546.6 0 65700 116 286 546.6 0 286 117 73 546.6 0 73 118 65 546.6 0 65 119 62 546.6 0 62 120 79 546.6 0 79 121 98 546.6 0 98 122 220 546.6 0 220 123 53 546.6 0 53 124 66 546.6 0 66 125 120 546.6 0 120 126 106 546.6 0 106 127 165 546.6 0 165 128 217 546.6 0 217 129 609 546.6 0 609 130 209 546.6 0 209 131 183 546.6 0 183 132 308 546.6 0 308 133 389 546.6 0 389 134 357 546.6 0 357 135 2046 546.6 0 2046 136 15309 546.6 0 15309 137 27469 546.6 0 27469 138 160 546.6 0 160 139 173 546.6 0 173 140 290 546.6 0 290 141 309 546.6 0 309 142 109 546.6 0 109 143 74 546.6 0 74 144 58 546.6 0 58 145 185 546.6 0 185 146 60 546.6 0 60 147 109 546.6 0 109 148 51 546.6 0 51 149 55 546.6 0 55 150 45 546.6 0 45 151 41 546.6 0 41 152 43 546.6 0 43 153 43 546.6 0 43 154 50 546.6 0 50 155 48 546.6 0 48 156 46 546.6 0 46 157 60 546.6 0 60 158 83 546.6 0 83 159 542 546.6 0 542 160 261 546.6 0 261 161 43 546.6 0 43 162 36 546.6 0 36 163 30 546.6 0 30 164 32 546.6 0 32 165 37 546.6 0 37 166 30 546.6 0 30 167 43 546.6 0 43 168 46 546.6 0 46 169 38 546.6 0 38 170 56 546.6 0 56 171 39 546.6 0 39 172 25 546.6 0 25 173 36 546.6 0 36 174 35 546.6 0 35 175 44 546.6 0 44 176 42 546.6 0 42 177 52 546.6 0 52 178 40 546.6 0 40 179 125 546.6 0 125 180 330 546.6 0 330 181 152 546.6 0 152 182 77 546.6 0 77 183 29 546.6 0 29 184 36 546.6 0 36 185 37 546.6 0 37 186 36 546.6 0 36 187 41 546.6 0 41 188 51 546.6 0 51 189 46 546.6 0 46 190 44 546.6 0 44 191 54 546.6 0 54 192 36 546.6 0 36 193 43 546.6 0 43 194 36 546.6 0 36 195 25 546.6 0 25 196 41 546.6 0 41 197 27 546.6 0 27 198 39 546.6 0 39 199 45 546.6 0 45 200 38 546.6 0 38 201 61 546.6 0 61 202 51 546.6 0 51 203 65 546.6 0 65 204 57 546.6 0 57 205 57 546.6 0 57 206 112 546.6 0 112 207 40 546.6 0 40 208 62 546.6 0 62 209 33 546.6 0 33 210 35 546.6 0 35 211 43 546.6 0 43 212 35 546.6 0 35 213 40 546.6 0 40 214 36 546.6 0 36 215 79 546.6 0 79 216 40 546.6 0 40 217 45 546.6 0 45 218 29 546.6 0 29 219 40 546.6 0 40 220 86 546.6 0 86 221 91 546.6 0 91 222 42 546.6 0 42 223 67 546.6 0 67 224 358 546.6 0 358 225 108 546.6 0 108 226 730 546.6 0 730 227 46 546.6 0 46 228 30 546.6 0 30 229 44 546.6 0 44 230 26 546.6 0 26 231 26 546.6 0 26 232 33 546.6 0 33 233 51 546.6 0 51 234 66 546.6 0 66 235 54 546.6 0 54 236 53 546.6 0 53 237 37 546.6 0 37 238 22 546.6 0 22 239 27 546.6 0 27 240 37 546.6 0 37 241 45 546.6 0 45 242 33 546.6 0 33 243 37 546.6 0 37 244 30 546.6 0 30 245 47 546.6 0 47 246 29 546.6 0 29 247 46 546.6 0 46 248 22 546.6 0 22 249 53 546.6 0 53 250 32 546.6 0 32 251 114 546.6 0 114 252 19 546.6 0 19 253 35 546.6 0 35 254 28 546.6 0 28 255 44 546.6 0 44 256 41 546.6 0 41 257 49 546.6 0 49 258 48 546.6 0 48 259 52 546.6 0 52 260 45 546.6 0 45 261 39 546.6 0 39 262 32 546.6 0 32 263 28 546.6 0 28 264 40 546.6 0 40 265 37 546.6 0 37 266 34 546.6 0 34 267 27 546.6 0 27 268 48 546.6 0 48 269 46 546.6 0 46 270 49 546.6 0 49 271 149 546.6 0 149 272 67 546.6 0 67 273 265 546.6 0 265 274 81 546.6 0 81 275 62 546.6 0 62 276 76 546.6 0 76 277 71 546.6 0 71 278 61 546.6 0 61 279 47 546.6 0 47 280 55 546.6 0 55 281 61 546.6 0 61 282 100 546.6 0 100 283 42 546.6 0 42 284 46 546.6 0 46 285 39 546.6 0 39 286 37 546.6 0 37 287 39 546.6 0 39 288 27 546.6 0 27 289 41 546.6 0 41 290 21 546.6 0 21 291 41 546.6 0 41 292 34 546.6 0 34 293 48 546.6 0 48 294 59 546.6 0 59 295 163 546.6 0 163 296 72 546.6 0 72 297 59 546.6 0 59 298 32 546.6 0 32 299 32 546.6 0 32 300 21 546.6 0 21 301 13 546.6 0 13 Finished in 11.45 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 351,496 Reads with adapters: 453 (0.1%) Reads that were too short: 386 (0.1%) Reads written (passing filters): 67 (0.0%) Total basepairs processed: 65,072,842 bp Total written (filtered): 9,700 bp (0.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 453 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 1 19 8 0.0 3 8 21 2 0.0 3 1 0 0 1 33 18 0.0 3 0 0 0 18 36 3 0.0 3 0 0 0 3 43 3 0.0 3 0 0 0 3 46 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 2 0.0 3 0 0 0 2 58 5 0.0 3 0 0 0 5 60 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 74 1 0.0 3 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 100 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 206 0.0 3 0 0 5 201 112 2 0.0 3 0 0 0 2 115 8 0.0 3 0 0 0 8 116 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 1 132 2 0.0 3 0 0 1 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 67 0.0 3 0 0 56 11 157 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 13 0.0 3 0 0 0 13 174 24 0.0 3 0 0 0 24 175 12 0.0 3 0 0 0 12 176 23 0.0 3 0 0 1 22 177 10 0.0 3 0 0 1 9 179 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (149 us/read; 0.40 M reads/minute). === Summary === Total reads processed: 67 Reads with adapters: 2 (3.0%) Reads written (passing filters): 67 (100.0%) Total basepairs processed: 9,700 bp Total written (filtered): 9,694 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 1.0 0 2