This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.49 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 513,525 (22.9%) Reads that were too short: 62,666 (2.8%) Reads written (passing filters): 450,859 (20.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 101,430,575 bp (15.1%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 513525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5095 546.6 0 5095 7 54603 546.6 0 54603 8 1072 546.6 0 1072 9 68376 546.6 0 68376 10 441 546.6 0 441 11 34 546.6 0 34 12 34 546.6 0 34 13 30 546.6 0 30 14 50 546.6 0 50 15 53 546.6 0 53 16 73 546.6 0 73 17 67 546.6 0 67 18 59 546.6 0 59 19 82 546.6 0 82 20 84 546.6 0 84 21 49 546.6 0 49 22 66 546.6 0 66 23 49 546.6 0 49 24 57 546.6 0 57 25 52 546.6 0 52 26 39 546.6 0 39 27 47 546.6 0 47 28 44 546.6 0 44 29 60 546.6 0 60 30 42 546.6 0 42 31 52 546.6 0 52 32 36 546.6 0 36 33 38 546.6 0 38 34 70 546.6 0 70 35 89 546.6 0 89 36 93 546.6 0 93 37 60 546.6 0 60 38 64 546.6 0 64 39 47 546.6 0 47 40 54 546.6 0 54 41 110 546.6 0 110 42 129 546.6 0 129 43 92 546.6 0 92 44 66 546.6 0 66 45 107 546.6 0 107 46 96 546.6 0 96 47 168 546.6 0 168 48 109 546.6 0 109 49 251 546.6 0 251 50 1192 546.6 0 1192 51 606 546.6 0 606 52 70 546.6 0 70 53 122 546.6 0 122 54 107 546.6 0 107 55 107 546.6 0 107 56 67 546.6 0 67 57 75 546.6 0 75 58 120 546.6 0 120 59 203 546.6 0 203 60 1516 546.6 0 1516 61 1192 546.6 0 1192 62 2943 546.6 0 2943 63 139 546.6 0 139 64 66 546.6 0 66 65 122 546.6 0 122 66 87 546.6 0 87 67 190 546.6 0 190 68 236 546.6 0 236 69 329 546.6 0 329 70 499 546.6 0 499 71 977 546.6 0 977 72 1408 546.6 0 1408 73 661 546.6 0 661 74 494 546.6 0 494 75 1039 546.6 0 1039 76 430 546.6 0 430 77 383 546.6 0 383 78 727 546.6 0 727 79 726 546.6 0 726 80 964 546.6 0 964 81 1081 546.6 0 1081 82 1447 546.6 0 1447 83 2169 546.6 0 2169 84 2503 546.6 0 2503 85 45978 546.6 0 45978 86 55493 546.6 0 55493 87 119488 546.6 0 119488 88 471 546.6 0 471 89 43 546.6 0 43 90 49 546.6 0 49 91 55 546.6 0 55 92 59 546.6 0 59 93 43 546.6 0 43 94 49 546.6 0 49 95 42 546.6 0 42 96 55 546.6 0 55 97 37 546.6 0 37 98 43 546.6 0 43 99 48 546.6 0 48 100 42 546.6 0 42 101 28 546.6 0 28 102 41 546.6 0 41 103 51 546.6 0 51 104 24 546.6 0 24 105 31 546.6 0 31 106 44 546.6 0 44 107 58 546.6 0 58 108 56 546.6 0 56 109 51 546.6 0 51 110 39 546.6 0 39 111 38 546.6 0 38 112 29 546.6 0 29 113 34 546.6 0 34 114 45 546.6 0 45 115 41 546.6 0 41 116 44 546.6 0 44 117 43 546.6 0 43 118 47 546.6 0 47 119 39 546.6 0 39 120 54 546.6 0 54 121 77 546.6 0 77 122 74 546.6 0 74 123 130 546.6 0 130 124 138 546.6 0 138 125 61 546.6 0 61 126 51 546.6 0 51 127 57 546.6 0 57 128 69 546.6 0 69 129 103 546.6 0 103 130 182 546.6 0 182 131 495 546.6 0 495 132 772 546.6 0 772 133 109 546.6 0 109 134 102 546.6 0 102 135 155 546.6 0 155 136 110 546.6 0 110 137 103 546.6 0 103 138 81 546.6 0 81 139 69 546.6 0 69 140 91 546.6 0 91 141 88 546.6 0 88 142 86 546.6 0 86 143 109 546.6 0 109 144 86 546.6 0 86 145 86 546.6 0 86 146 107 546.6 0 107 147 89 546.6 0 89 148 141 546.6 0 141 149 217 546.6 0 217 150 356 546.6 0 356 151 434 546.6 0 434 152 407 546.6 0 407 153 2185 546.6 0 2185 154 2067 546.6 0 2067 155 2020 546.6 0 2020 156 352 546.6 0 352 157 102 546.6 0 102 158 69 546.6 0 69 159 91 546.6 0 91 160 91 546.6 0 91 161 140 546.6 0 140 162 230 546.6 0 230 163 89 546.6 0 89 164 135 546.6 0 135 165 195 546.6 0 195 166 113 546.6 0 113 167 115 546.6 0 115 168 308 546.6 0 308 169 218 546.6 0 218 170 273 546.6 0 273 171 343 546.6 0 343 172 447 546.6 0 447 173 767 546.6 0 767 174 1180 546.6 0 1180 175 8567 546.6 0 8567 176 24061 546.6 0 24061 177 19102 546.6 0 19102 178 130 546.6 0 130 179 53 546.6 0 53 180 80 546.6 0 80 181 137 546.6 0 137 182 101 546.6 0 101 183 156 546.6 0 156 184 80 546.6 0 80 185 81 546.6 0 81 186 102 546.6 0 102 187 111 546.6 0 111 188 55 546.6 0 55 189 108 546.6 0 108 190 75 546.6 0 75 191 107 546.6 0 107 192 77 546.6 0 77 193 59 546.6 0 59 194 80 546.6 0 80 195 64 546.6 0 64 196 84 546.6 0 84 197 66 546.6 0 66 198 80 546.6 0 80 199 71 546.6 0 71 200 69 546.6 0 69 201 120 546.6 0 120 202 71 546.6 0 71 203 87 546.6 0 87 204 329 546.6 0 329 205 140 546.6 0 140 206 157 546.6 0 157 207 91 546.6 0 91 208 152 546.6 0 152 209 193 546.6 0 193 210 354 546.6 0 354 211 515 546.6 0 515 212 1412 546.6 0 1412 213 22901 546.6 0 22901 214 1147 546.6 0 1147 215 11591 546.6 0 11591 216 208 546.6 0 208 217 193 546.6 0 193 218 3279 546.6 0 3279 219 98 546.6 0 98 220 102 546.6 0 102 221 112 546.6 0 112 222 154 546.6 0 154 223 44 546.6 0 44 224 67 546.6 0 67 225 80 546.6 0 80 226 85 546.6 0 85 227 94 546.6 0 94 228 189 546.6 0 189 229 178 546.6 0 178 230 219 546.6 0 219 231 198 546.6 0 198 232 362 546.6 0 362 233 253 546.6 0 253 234 211 546.6 0 211 235 119 546.6 0 119 236 77 546.6 0 77 237 97 546.6 0 97 238 99 546.6 0 99 239 84 546.6 0 84 240 97 546.6 0 97 241 87 546.6 0 87 242 66 546.6 0 66 243 76 546.6 0 76 244 61 546.6 0 61 245 78 546.6 0 78 246 99 546.6 0 99 247 227 546.6 0 227 248 247 546.6 0 247 249 297 546.6 0 297 250 219 546.6 0 219 251 184 546.6 0 184 252 183 546.6 0 183 253 1020 546.6 0 1020 254 722 546.6 0 722 255 1536 546.6 0 1536 256 417 546.6 0 417 257 220 546.6 0 220 258 294 546.6 0 294 259 155 546.6 0 155 260 88 546.6 0 88 261 94 546.6 0 94 262 78 546.6 0 78 263 64 546.6 0 64 264 62 546.6 0 62 265 52 546.6 0 52 266 86 546.6 0 86 267 92 546.6 0 92 268 124 546.6 0 124 269 142 546.6 0 142 270 105 546.6 0 105 271 136 546.6 0 136 272 96 546.6 0 96 273 68 546.6 0 68 274 88 546.6 0 88 275 129 546.6 0 129 276 113 546.6 0 113 277 212 546.6 0 212 278 307 546.6 0 307 279 432 546.6 0 432 280 353 546.6 0 353 281 163 546.6 0 163 282 145 546.6 0 145 283 155 546.6 0 155 284 109 546.6 0 109 285 876 546.6 0 876 286 305 546.6 0 305 287 828 546.6 0 828 288 177 546.6 0 177 289 208 546.6 0 208 290 256 546.6 0 256 291 138 546.6 0 138 292 160 546.6 0 160 293 333 546.6 0 333 294 361 546.6 0 361 295 336 546.6 0 336 296 354 546.6 0 354 297 856 546.6 0 856 298 755 546.6 0 755 299 589 546.6 0 589 300 359 546.6 0 359 301 255 546.6 0 255 Finished in 19.39 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 450,859 Reads with adapters: 58,935 (13.1%) Reads that were too short: 299 (0.1%) Reads written (passing filters): 58,636 (13.0%) Total basepairs processed: 101,430,575 bp Total written (filtered): 16,045,372 bp (15.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 58935 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 13 0.0 3 0 0 9 1 3 19 293 0.0 3 0 230 37 21 5 20 57884 0.0 4 55043 2354 380 80 27 21 121 0.0 4 14 94 7 4 2 22 17 0.0 4 3 0 11 2 1 23 86 0.0 4 3 0 0 62 21 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 1 27 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 33 2 0.0 4 1 0 0 0 1 40 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 1 42 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 93 0.0 4 0 0 0 0 93 50 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 1 58 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 62 4 0.0 4 0 0 0 0 4 66 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 3 0.0 4 0 0 0 0 3 77 3 0.0 4 0 0 0 0 3 87 3 0.0 4 0 0 0 0 3 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 91 14 0.0 4 0 0 0 0 14 93 2 0.0 4 0 0 0 0 2 100 4 0.0 4 0 0 0 0 4 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 3 0.0 4 0 0 0 0 3 111 2 0.0 4 0 0 0 0 2 112 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 1 1 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 85 0.0 4 0 0 0 0 85 128 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 4 0.0 4 0 0 0 0 4 136 5 0.0 4 0 0 0 0 5 137 6 0.0 4 0 0 0 1 5 138 11 0.0 4 0 0 0 1 10 139 3 0.0 4 0 0 0 0 3 140 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 145 1 0.0 4 1 146 3 0.0 4 0 0 0 0 3 147 1 0.0 4 0 0 0 0 1 150 39 0.0 4 0 0 0 1 38 151 24 0.0 4 0 0 0 0 24 152 7 0.0 4 0 0 0 0 7 153 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 1 156 2 0.0 4 0 0 0 0 2 157 3 0.0 4 0 0 0 1 2 159 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 2 0.0 4 0 0 0 0 2 180 5 0.0 4 0 0 0 1 4 181 4 0.0 4 0 0 0 0 4 182 3 0.0 4 0 0 0 0 3 183 2 0.0 4 0 0 0 0 2 184 10 0.0 4 0 0 0 0 10 185 5 0.0 4 0 0 0 0 5 186 11 0.0 4 0 0 0 0 11 187 9 0.0 4 0 0 0 0 9 190 2 0.0 4 0 0 0 0 2 192 1 0.0 4 0 0 0 0 1 196 2 0.0 4 0 0 0 0 2 197 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 2 0.0 4 0 0 0 0 2 209 3 0.0 4 0 0 0 0 3 211 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 3 0.0 4 0 0 0 0 3 215 6 0.0 4 0 0 0 0 6 216 4 0.0 4 0 0 0 0 4 217 2 0.0 4 0 0 0 0 2 220 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 0 10 229 7 0.0 4 0 0 0 0 7 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 1 2 237 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 255 2 0.0 4 0 0 0 1 1 258 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 0 2 264 3 0.0 4 0 0 0 0 3 265 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.02 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 58,636 Reads with adapters: 2,988 (5.1%) Reads written (passing filters): 58,636 (100.0%) Total basepairs processed: 16,045,372 bp Total written (filtered): 16,003,526 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2988 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.9% C: 13.7% G: 76.1% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 230 916.2 0 230 4 33 229.0 0 33 5 2 57.3 0 2 6 2 14.3 0 2 9 12 0.2 0 1 11 10 72 0.1 1 4 68 11 1594 0.0 1 92 1502 12 746 0.0 1 33 713 13 213 0.0 1 41 172 14 14 0.0 1 3 11 15 8 0.0 1 0 8 17 1 0.0 1 0 1 152 34 0.0 1 25 9 153 4 0.0 1 3 1 179 2 0.0 1 2 191 1 0.0 1 0 1 209 1 0.0 1 0 1 210 14 0.0 1 11 3 211 1 0.0 1 1 222 1 0.0 1 1 225 3 0.0 1 3