This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.05 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 487,247 (21.8%) Reads that were too short: 67,727 (3.0%) Reads written (passing filters): 419,520 (18.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 94,446,747 bp (14.0%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 487247 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5295 546.6 0 5295 7 68121 546.6 0 68121 8 553 546.6 0 553 9 59281 546.6 0 59281 10 113 546.6 0 113 11 52 546.6 0 52 12 55 546.6 0 55 13 45 546.6 0 45 14 34 546.6 0 34 15 70 546.6 0 70 16 68 546.6 0 68 17 69 546.6 0 69 18 42 546.6 0 42 19 59 546.6 0 59 20 79 546.6 0 79 21 72 546.6 0 72 22 70 546.6 0 70 23 53 546.6 0 53 24 77 546.6 0 77 25 53 546.6 0 53 26 52 546.6 0 52 27 59 546.6 0 59 28 61 546.6 0 61 29 84 546.6 0 84 30 53 546.6 0 53 31 54 546.6 0 54 32 75 546.6 0 75 33 70 546.6 0 70 34 71 546.6 0 71 35 89 546.6 0 89 36 76 546.6 0 76 37 60 546.6 0 60 38 73 546.6 0 73 39 55 546.6 0 55 40 72 546.6 0 72 41 137 546.6 0 137 42 109 546.6 0 109 43 98 546.6 0 98 44 64 546.6 0 64 45 99 546.6 0 99 46 74 546.6 0 74 47 97 546.6 0 97 48 62 546.6 0 62 49 86 546.6 0 86 50 230 546.6 0 230 51 180 546.6 0 180 52 79 546.6 0 79 53 181 546.6 0 181 54 64 546.6 0 64 55 96 546.6 0 96 56 64 546.6 0 64 57 67 546.6 0 67 58 82 546.6 0 82 59 160 546.6 0 160 60 412 546.6 0 412 61 486 546.6 0 486 62 1055 546.6 0 1055 63 112 546.6 0 112 64 82 546.6 0 82 65 112 546.6 0 112 66 77 546.6 0 77 67 164 546.6 0 164 68 170 546.6 0 170 69 243 546.6 0 243 70 336 546.6 0 336 71 931 546.6 0 931 72 1375 546.6 0 1375 73 681 546.6 0 681 74 462 546.6 0 462 75 995 546.6 0 995 76 402 546.6 0 402 77 364 546.6 0 364 78 658 546.6 0 658 79 647 546.6 0 647 80 818 546.6 0 818 81 903 546.6 0 903 82 1082 546.6 0 1082 83 1484 546.6 0 1484 84 1719 546.6 0 1719 85 39938 546.6 0 39938 86 49867 546.6 0 49867 87 100431 546.6 0 100431 88 369 546.6 0 369 89 60 546.6 0 60 90 78 546.6 0 78 91 70 546.6 0 70 92 51 546.6 0 51 93 57 546.6 0 57 94 64 546.6 0 64 95 63 546.6 0 63 96 68 546.6 0 68 97 48 546.6 0 48 98 59 546.6 0 59 99 52 546.6 0 52 100 48 546.6 0 48 101 50 546.6 0 50 102 54 546.6 0 54 103 45 546.6 0 45 104 53 546.6 0 53 105 65 546.6 0 65 106 68 546.6 0 68 107 73 546.6 0 73 108 73 546.6 0 73 109 74 546.6 0 74 110 61 546.6 0 61 111 63 546.6 0 63 112 51 546.6 0 51 113 44 546.6 0 44 114 49 546.6 0 49 115 44 546.6 0 44 116 46 546.6 0 46 117 50 546.6 0 50 118 48 546.6 0 48 119 31 546.6 0 31 120 49 546.6 0 49 121 55 546.6 0 55 122 66 546.6 0 66 123 49 546.6 0 49 124 57 546.6 0 57 125 77 546.6 0 77 126 61 546.6 0 61 127 41 546.6 0 41 128 56 546.6 0 56 129 75 546.6 0 75 130 84 546.6 0 84 131 217 546.6 0 217 132 327 546.6 0 327 133 68 546.6 0 68 134 55 546.6 0 55 135 80 546.6 0 80 136 67 546.6 0 67 137 57 546.6 0 57 138 62 546.6 0 62 139 78 546.6 0 78 140 76 546.6 0 76 141 65 546.6 0 65 142 72 546.6 0 72 143 110 546.6 0 110 144 54 546.6 0 54 145 77 546.6 0 77 146 100 546.6 0 100 147 88 546.6 0 88 148 98 546.6 0 98 149 198 546.6 0 198 150 262 546.6 0 262 151 397 546.6 0 397 152 300 546.6 0 300 153 1658 546.6 0 1658 154 1493 546.6 0 1493 155 1400 546.6 0 1400 156 171 546.6 0 171 157 122 546.6 0 122 158 74 546.6 0 74 159 89 546.6 0 89 160 94 546.6 0 94 161 174 546.6 0 174 162 303 546.6 0 303 163 103 546.6 0 103 164 137 546.6 0 137 165 209 546.6 0 209 166 102 546.6 0 102 167 150 546.6 0 150 168 366 546.6 0 366 169 203 546.6 0 203 170 267 546.6 0 267 171 359 546.6 0 359 172 431 546.6 0 431 173 750 546.6 0 750 174 882 546.6 0 882 175 8811 546.6 0 8811 176 28256 546.6 0 28256 177 22197 546.6 0 22197 178 131 546.6 0 131 179 52 546.6 0 52 180 66 546.6 0 66 181 70 546.6 0 70 182 64 546.6 0 64 183 78 546.6 0 78 184 91 546.6 0 91 185 52 546.6 0 52 186 89 546.6 0 89 187 88 546.6 0 88 188 50 546.6 0 50 189 118 546.6 0 118 190 54 546.6 0 54 191 72 546.6 0 72 192 34 546.6 0 34 193 44 546.6 0 44 194 54 546.6 0 54 195 46 546.6 0 46 196 94 546.6 0 94 197 63 546.6 0 63 198 67 546.6 0 67 199 48 546.6 0 48 200 69 546.6 0 69 201 118 546.6 0 118 202 45 546.6 0 45 203 52 546.6 0 52 204 137 546.6 0 137 205 60 546.6 0 60 206 91 546.6 0 91 207 108 546.6 0 108 208 147 546.6 0 147 209 236 546.6 0 236 210 353 546.6 0 353 211 555 546.6 0 555 212 1653 546.6 0 1653 213 33264 546.6 0 33264 214 1308 546.6 0 1308 215 16487 546.6 0 16487 216 159 546.6 0 159 217 180 546.6 0 180 218 4414 546.6 0 4414 219 71 546.6 0 71 220 48 546.6 0 48 221 61 546.6 0 61 222 179 546.6 0 179 223 42 546.6 0 42 224 31 546.6 0 31 225 40 546.6 0 40 226 35 546.6 0 35 227 45 546.6 0 45 228 88 546.6 0 88 229 80 546.6 0 80 230 83 546.6 0 83 231 120 546.6 0 120 232 278 546.6 0 278 233 174 546.6 0 174 234 118 546.6 0 118 235 64 546.6 0 64 236 51 546.6 0 51 237 56 546.6 0 56 238 56 546.6 0 56 239 44 546.6 0 44 240 73 546.6 0 73 241 36 546.6 0 36 242 45 546.6 0 45 243 57 546.6 0 57 244 40 546.6 0 40 245 40 546.6 0 40 246 68 546.6 0 68 247 80 546.6 0 80 248 81 546.6 0 81 249 91 546.6 0 91 250 61 546.6 0 61 251 76 546.6 0 76 252 79 546.6 0 79 253 276 546.6 0 276 254 197 546.6 0 197 255 386 546.6 0 386 256 117 546.6 0 117 257 95 546.6 0 95 258 153 546.6 0 153 259 91 546.6 0 91 260 45 546.6 0 45 261 55 546.6 0 55 262 42 546.6 0 42 263 38 546.6 0 38 264 50 546.6 0 50 265 52 546.6 0 52 266 49 546.6 0 49 267 52 546.6 0 52 268 49 546.6 0 49 269 40 546.6 0 40 270 48 546.6 0 48 271 65 546.6 0 65 272 50 546.6 0 50 273 49 546.6 0 49 274 62 546.6 0 62 275 44 546.6 0 44 276 42 546.6 0 42 277 216 546.6 0 216 278 243 546.6 0 243 279 392 546.6 0 392 280 77 546.6 0 77 281 59 546.6 0 59 282 44 546.6 0 44 283 53 546.6 0 53 284 56 546.6 0 56 285 322 546.6 0 322 286 129 546.6 0 129 287 297 546.6 0 297 288 79 546.6 0 79 289 109 546.6 0 109 290 115 546.6 0 115 291 79 546.6 0 79 292 67 546.6 0 67 293 129 546.6 0 129 294 152 546.6 0 152 295 103 546.6 0 103 296 115 546.6 0 115 297 262 546.6 0 262 298 269 546.6 0 269 299 155 546.6 0 155 300 126 546.6 0 126 301 92 546.6 0 92 Finished in 15.77 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 419,520 Reads with adapters: 63,769 (15.2%) Reads that were too short: 570 (0.1%) Reads written (passing filters): 63,199 (15.1%) Total basepairs processed: 94,446,747 bp Total written (filtered): 16,991,252 bp (18.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63769 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 224 0.0 3 0 220 4 17 60150 0.0 3 58510 1275 53 312 18 119 0.0 3 13 104 0 2 19 241 0.0 3 215 1 23 2 20 121 0.0 3 0 0 0 121 22 1 0.0 3 1 25 1 0.0 3 1 28 1 0.0 3 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 1 35 2 0.0 3 2 36 1 0.0 3 0 0 0 1 37 360 0.0 3 0 0 0 360 38 19 0.0 3 0 0 0 19 39 3 0.0 3 0 0 0 3 41 5 0.0 3 0 0 0 5 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 1 2 65 54 0.0 3 0 0 0 54 66 1 0.0 3 0 1 67 5 0.0 3 0 0 0 5 68 3 0.0 3 0 0 0 3 69 2 0.0 3 1 0 0 1 77 12 0.0 3 0 0 0 12 81 3 0.0 3 0 0 0 3 82 4 0.0 3 0 0 0 4 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 4 0.0 3 0 0 0 4 86 707 0.0 3 0 0 3 704 87 204 0.0 3 0 0 1 203 88 320 0.0 3 0 0 2 318 89 275 0.0 3 0 0 3 272 90 41 0.0 3 0 0 0 41 91 64 0.0 3 0 0 3 61 92 20 0.0 3 0 0 0 20 93 4 0.0 3 1 0 0 3 94 7 0.0 3 0 0 0 7 95 3 0.0 3 0 0 0 3 96 3 0.0 3 0 0 0 3 97 6 0.0 3 0 0 0 6 98 3 0.0 3 0 0 0 3 99 8 0.0 3 0 0 0 8 100 9 0.0 3 0 0 0 9 101 7 0.0 3 0 0 0 7 102 7 0.0 3 0 0 0 7 103 7 0.0 3 0 0 0 7 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 13 0.0 3 0 0 0 13 107 61 0.0 3 0 0 0 61 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 11 0.0 3 0 0 0 11 112 15 0.0 3 0 0 0 15 113 4 0.0 3 0 0 0 4 114 5 0.0 3 0 0 0 5 115 2 0.0 3 0 0 0 2 118 5 0.0 3 0 0 0 5 119 2 0.0 3 0 0 0 2 124 3 0.0 3 0 0 0 3 127 2 0.0 3 0 0 0 2 131 33 0.0 3 0 0 0 33 132 3 0.0 3 0 0 0 3 133 1 0.0 3 1 134 7 0.0 3 0 0 0 7 137 4 0.0 3 0 0 0 4 138 3 0.0 3 0 0 0 3 139 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 4 0.0 3 0 0 0 4 146 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 21 0.0 3 0 0 0 21 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 183 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 8 0.0 3 0 0 0 8 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 140 0.0 3 0 0 0 140 199 24 0.0 3 0 0 1 23 200 30 0.0 3 0 0 0 30 201 64 0.0 3 0 0 0 64 202 52 0.0 3 0 0 2 50 203 35 0.0 3 0 0 0 35 204 18 0.0 3 0 0 1 17 205 4 0.0 3 0 0 0 4 206 11 0.0 3 0 0 0 11 207 4 0.0 3 0 0 0 4 208 5 0.0 3 0 0 0 5 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 211 3 0.0 3 0 0 0 3 212 2 0.0 3 0 0 0 2 213 3 0.0 3 0 0 0 3 214 3 0.0 3 0 0 0 3 215 4 0.0 3 0 0 0 4 216 15 0.0 3 0 0 0 15 217 34 0.0 3 0 0 2 32 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 232 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 3 0.0 3 0 0 0 3 281 3 0.0 3 0 0 0 3 282 4 0.0 3 0 0 1 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.18 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 63,199 Reads with adapters: 2,438 (3.9%) Reads written (passing filters): 63,199 (100.0%) Total basepairs processed: 16,991,252 bp Total written (filtered): 16,983,897 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2438 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.6% C: 9.4% G: 24.9% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2404 987.5 0 2404 4 29 246.9 0 29 5 3 61.7 0 3 6 2 15.4 0 2