This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.79 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 44,010 (2.0%) Reads that were too short: 7,710 (0.3%) Reads written (passing filters): 36,300 (1.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,710,155 bp (1.4%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 44010 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 232 546.6 0 232 7 291 546.6 0 291 8 14915 546.6 0 14915 9 12678 546.6 0 12678 10 20 546.6 0 20 11 7 546.6 0 7 12 7 546.6 0 7 13 2 546.6 0 2 14 3 546.6 0 3 15 4 546.6 0 4 16 5 546.6 0 5 17 2 546.6 0 2 18 2 546.6 0 2 19 4 546.6 0 4 20 3 546.6 0 3 21 3 546.6 0 3 22 9 546.6 0 9 23 21 546.6 0 21 24 3 546.6 0 3 25 6 546.6 0 6 26 2 546.6 0 2 27 5 546.6 0 5 28 2 546.6 0 2 29 10 546.6 0 10 30 13 546.6 0 13 31 5 546.6 0 5 32 6 546.6 0 6 33 26 546.6 0 26 34 27 546.6 0 27 35 24 546.6 0 24 36 17 546.6 0 17 37 5 546.6 0 5 38 56 546.6 0 56 39 21 546.6 0 21 40 14 546.6 0 14 41 4 546.6 0 4 43 3 546.6 0 3 44 6 546.6 0 6 45 5 546.6 0 5 46 3 546.6 0 3 47 1 546.6 0 1 48 6 546.6 0 6 50 7 546.6 0 7 51 4 546.6 0 4 52 7 546.6 0 7 53 10 546.6 0 10 54 3 546.6 0 3 55 6 546.6 0 6 56 9 546.6 0 9 57 5 546.6 0 5 58 49 546.6 0 49 59 23 546.6 0 23 60 82 546.6 0 82 61 2 546.6 0 2 62 6 546.6 0 6 63 8 546.6 0 8 64 8 546.6 0 8 65 7 546.6 0 7 66 7 546.6 0 7 67 18 546.6 0 18 68 4 546.6 0 4 69 8 546.6 0 8 70 18 546.6 0 18 71 60 546.6 0 60 72 8 546.6 0 8 73 15 546.6 0 15 74 27 546.6 0 27 75 14 546.6 0 14 76 68 546.6 0 68 77 427 546.6 0 427 78 479 546.6 0 479 79 7 546.6 0 7 80 157 546.6 0 157 81 83 546.6 0 83 82 286 546.6 0 286 83 17 546.6 0 17 84 8 546.6 0 8 85 22 546.6 0 22 86 8 546.6 0 8 87 8 546.6 0 8 88 22 546.6 0 22 89 12 546.6 0 12 90 11 546.6 0 11 91 19 546.6 0 19 92 6 546.6 0 6 93 20 546.6 0 20 94 18 546.6 0 18 95 10 546.6 0 10 96 20 546.6 0 20 97 14 546.6 0 14 98 9 546.6 0 9 99 43 546.6 0 43 100 640 546.6 0 640 101 708 546.6 0 708 102 632 546.6 0 632 103 12 546.6 0 12 104 6 546.6 0 6 105 6 546.6 0 6 106 8 546.6 0 8 107 7 546.6 0 7 108 14 546.6 0 14 109 116 546.6 0 116 110 228 546.6 0 228 111 177 546.6 0 177 112 136 546.6 0 136 113 9 546.6 0 9 114 6 546.6 0 6 115 4 546.6 0 4 116 5 546.6 0 5 117 13 546.6 0 13 118 9 546.6 0 9 119 4 546.6 0 4 120 12 546.6 0 12 121 7 546.6 0 7 122 6 546.6 0 6 123 8 546.6 0 8 124 3 546.6 0 3 125 4 546.6 0 4 126 14 546.6 0 14 127 9 546.6 0 9 128 23 546.6 0 23 129 13 546.6 0 13 130 13 546.6 0 13 131 23 546.6 0 23 132 31 546.6 0 31 133 22 546.6 0 22 134 11 546.6 0 11 135 16 546.6 0 16 136 10 546.6 0 10 137 19 546.6 0 19 138 8 546.6 0 8 139 2 546.6 0 2 140 17 546.6 0 17 141 7 546.6 0 7 142 19 546.6 0 19 143 6 546.6 0 6 144 25 546.6 0 25 145 19 546.6 0 19 146 9 546.6 0 9 147 43 546.6 0 43 148 38 546.6 0 38 149 29 546.6 0 29 150 37 546.6 0 37 151 95 546.6 0 95 152 47 546.6 0 47 153 24 546.6 0 24 154 8 546.6 0 8 155 6 546.6 0 6 156 9 546.6 0 9 157 27 546.6 0 27 158 48 546.6 0 48 159 46 546.6 0 46 160 11 546.6 0 11 161 12 546.6 0 12 162 15 546.6 0 15 163 9 546.6 0 9 164 11 546.6 0 11 165 8 546.6 0 8 166 10 546.6 0 10 167 14 546.6 0 14 168 60 546.6 0 60 169 22 546.6 0 22 170 137 546.6 0 137 171 7 546.6 0 7 172 6 546.6 0 6 173 8 546.6 0 8 174 8 546.6 0 8 175 8 546.6 0 8 176 11 546.6 0 11 177 17 546.6 0 17 178 16 546.6 0 16 179 14 546.6 0 14 180 17 546.6 0 17 181 18 546.6 0 18 182 31 546.6 0 31 183 38 546.6 0 38 184 93 546.6 0 93 185 33 546.6 0 33 186 50 546.6 0 50 187 38 546.6 0 38 188 12 546.6 0 12 189 14 546.6 0 14 190 17 546.6 0 17 191 20 546.6 0 20 192 26 546.6 0 26 193 23 546.6 0 23 194 13 546.6 0 13 195 33 546.6 0 33 196 86 546.6 0 86 197 58 546.6 0 58 198 77 546.6 0 77 199 191 546.6 0 191 200 318 546.6 0 318 201 470 546.6 0 470 202 212 546.6 0 212 203 175 546.6 0 175 204 485 546.6 0 485 205 98 546.6 0 98 206 57 546.6 0 57 207 45 546.6 0 45 208 40 546.6 0 40 209 24 546.6 0 24 210 27 546.6 0 27 211 23 546.6 0 23 212 43 546.6 0 43 213 47 546.6 0 47 214 25 546.6 0 25 215 36 546.6 0 36 216 28 546.6 0 28 217 54 546.6 0 54 218 143 546.6 0 143 219 191 546.6 0 191 220 74 546.6 0 74 221 53 546.6 0 53 222 1039 546.6 0 1039 223 286 546.6 0 286 224 1142 546.6 0 1142 225 86 546.6 0 86 226 31 546.6 0 31 227 17 546.6 0 17 228 22 546.6 0 22 229 30 546.6 0 30 230 18 546.6 0 18 231 22 546.6 0 22 232 30 546.6 0 30 233 17 546.6 0 17 234 18 546.6 0 18 235 29 546.6 0 29 236 26 546.6 0 26 237 24 546.6 0 24 238 37 546.6 0 37 239 34 546.6 0 34 240 29 546.6 0 29 241 49 546.6 0 49 242 46 546.6 0 46 243 65 546.6 0 65 244 24 546.6 0 24 245 26 546.6 0 26 246 20 546.6 0 20 247 17 546.6 0 17 248 17 546.6 0 17 249 19 546.6 0 19 250 31 546.6 0 31 251 48 546.6 0 48 252 28 546.6 0 28 253 33 546.6 0 33 254 36 546.6 0 36 255 52 546.6 0 52 256 69 546.6 0 69 257 114 546.6 0 114 258 79 546.6 0 79 259 43 546.6 0 43 260 53 546.6 0 53 261 26 546.6 0 26 262 63 546.6 0 63 263 54 546.6 0 54 264 79 546.6 0 79 265 53 546.6 0 53 266 63 546.6 0 63 267 25 546.6 0 25 268 28 546.6 0 28 269 30 546.6 0 30 270 30 546.6 0 30 271 53 546.6 0 53 272 36 546.6 0 36 273 48 546.6 0 48 274 36 546.6 0 36 275 35 546.6 0 35 276 72 546.6 0 72 277 71 546.6 0 71 278 119 546.6 0 119 279 46 546.6 0 46 280 69 546.6 0 69 281 25 546.6 0 25 282 44 546.6 0 44 283 48 546.6 0 48 284 35 546.6 0 35 285 50 546.6 0 50 286 27 546.6 0 27 287 33 546.6 0 33 288 41 546.6 0 41 289 42 546.6 0 42 290 46 546.6 0 46 291 73 546.6 0 73 292 88 546.6 0 88 293 58 546.6 0 58 294 54 546.6 0 54 295 37 546.6 0 37 296 26 546.6 0 26 297 52 546.6 0 52 298 55 546.6 0 55 299 54 546.6 0 54 300 66 546.6 0 66 301 34 546.6 0 34 Finished in 1.83 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 36,300 Reads with adapters: 12,963 (35.7%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 12,947 (35.7%) Total basepairs processed: 9,710,155 bp Total written (filtered): 3,521,015 bp (36.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12963 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 7 0.0 3 0 0 2 1 4 19 61 0.0 3 0 42 7 8 4 20 12742 0.0 4 11963 622 118 32 7 21 32 0.0 4 6 23 1 1 1 22 4 0.0 4 0 0 2 1 1 23 85 0.0 4 1 3 0 68 13 24 3 0.0 4 2 0 0 0 1 44 1 0.0 4 0 0 0 0 1 68 1 0.0 4 1 69 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 223 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 1 237 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 12,947 Reads with adapters: 3,110 (24.0%) Reads written (passing filters): 12,947 (100.0%) Total basepairs processed: 3,521,015 bp Total written (filtered): 3,488,268 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3110 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 8.1% G: 87.8% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 202.3 0 3 4 2 50.6 0 2 9 521 0.0 0 51 470 10 2239 0.0 1 170 2069 11 318 0.0 1 11 307 12 8 0.0 1 0 8 13 8 0.0 1 0 8 72 1 0.0 1 1 150 4 0.0 1 3 1 208 3 0.0 1 3 210 1 0.0 1 1 223 1 0.0 1 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.