This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.49 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 36,076 (1.6%) Reads that were too short: 2,259 (0.1%) Reads written (passing filters): 33,817 (1.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 9,471,914 bp (1.4%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 36076 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 546.6 0 208 7 257 546.6 0 257 8 12552 546.6 0 12552 9 16507 546.6 0 16507 10 13 546.6 0 13 11 4 546.6 0 4 12 2 546.6 0 2 13 2 546.6 0 2 14 3 546.6 0 3 15 3 546.6 0 3 16 3 546.6 0 3 17 2 546.6 0 2 18 1 546.6 0 1 19 4 546.6 0 4 20 3 546.6 0 3 23 2 546.6 0 2 25 1 546.6 0 1 26 2 546.6 0 2 27 2 546.6 0 2 29 4 546.6 0 4 30 2 546.6 0 2 31 5 546.6 0 5 32 4 546.6 0 4 33 4 546.6 0 4 34 6 546.6 0 6 35 21 546.6 0 21 36 1 546.6 0 1 37 3 546.6 0 3 38 29 546.6 0 29 39 18 546.6 0 18 40 10 546.6 0 10 41 1 546.6 0 1 42 2 546.6 0 2 43 2 546.6 0 2 44 3 546.6 0 3 45 4 546.6 0 4 46 2 546.6 0 2 47 1 546.6 0 1 48 4 546.6 0 4 49 2 546.6 0 2 50 3 546.6 0 3 51 1 546.6 0 1 52 4 546.6 0 4 53 1 546.6 0 1 54 3 546.6 0 3 55 6 546.6 0 6 56 4 546.6 0 4 57 6 546.6 0 6 58 54 546.6 0 54 59 21 546.6 0 21 60 47 546.6 0 47 61 2 546.6 0 2 62 1 546.6 0 1 63 4 546.6 0 4 64 3 546.6 0 3 65 2 546.6 0 2 66 2 546.6 0 2 67 4 546.6 0 4 68 3 546.6 0 3 69 9 546.6 0 9 70 8 546.6 0 8 71 62 546.6 0 62 72 11 546.6 0 11 73 13 546.6 0 13 74 16 546.6 0 16 75 11 546.6 0 11 76 50 546.6 0 50 77 411 546.6 0 411 78 390 546.6 0 390 79 5 546.6 0 5 80 42 546.6 0 42 81 33 546.6 0 33 82 100 546.6 0 100 83 3 546.6 0 3 84 1 546.6 0 1 85 5 546.6 0 5 87 4 546.6 0 4 88 4 546.6 0 4 89 5 546.6 0 5 90 6 546.6 0 6 91 6 546.6 0 6 92 8 546.6 0 8 93 2 546.6 0 2 94 2 546.6 0 2 95 12 546.6 0 12 96 4 546.6 0 4 97 4 546.6 0 4 98 8 546.6 0 8 99 22 546.6 0 22 100 366 546.6 0 366 101 356 546.6 0 356 102 276 546.6 0 276 103 9 546.6 0 9 104 5 546.6 0 5 105 6 546.6 0 6 106 5 546.6 0 5 107 6 546.6 0 6 108 15 546.6 0 15 109 121 546.6 0 121 110 248 546.6 0 248 111 165 546.6 0 165 112 133 546.6 0 133 113 2 546.6 0 2 114 4 546.6 0 4 115 18 546.6 0 18 116 5 546.6 0 5 117 2 546.6 0 2 118 5 546.6 0 5 119 3 546.6 0 3 120 3 546.6 0 3 121 4 546.6 0 4 122 8 546.6 0 8 123 2 546.6 0 2 124 2 546.6 0 2 125 4 546.6 0 4 126 9 546.6 0 9 127 4 546.6 0 4 128 26 546.6 0 26 129 7 546.6 0 7 130 7 546.6 0 7 131 14 546.6 0 14 132 4 546.6 0 4 133 3 546.6 0 3 134 3 546.6 0 3 135 1 546.6 0 1 136 4 546.6 0 4 137 11 546.6 0 11 138 8 546.6 0 8 139 7 546.6 0 7 140 6 546.6 0 6 141 6 546.6 0 6 142 2 546.6 0 2 143 5 546.6 0 5 144 1 546.6 0 1 145 2 546.6 0 2 146 2 546.6 0 2 147 23 546.6 0 23 148 19 546.6 0 19 149 16 546.6 0 16 150 16 546.6 0 16 151 83 546.6 0 83 152 31 546.6 0 31 153 7 546.6 0 7 154 4 546.6 0 4 155 4 546.6 0 4 156 7 546.6 0 7 157 7 546.6 0 7 158 7 546.6 0 7 159 7 546.6 0 7 160 6 546.6 0 6 161 7 546.6 0 7 162 6 546.6 0 6 163 6 546.6 0 6 164 3 546.6 0 3 165 2 546.6 0 2 166 2 546.6 0 2 167 7 546.6 0 7 168 50 546.6 0 50 169 10 546.6 0 10 170 129 546.6 0 129 171 5 546.6 0 5 172 4 546.6 0 4 174 2 546.6 0 2 176 3 546.6 0 3 177 9 546.6 0 9 178 6 546.6 0 6 179 8 546.6 0 8 180 6 546.6 0 6 181 5 546.6 0 5 182 6 546.6 0 6 183 8 546.6 0 8 184 9 546.6 0 9 185 7 546.6 0 7 186 12 546.6 0 12 187 3 546.6 0 3 188 5 546.6 0 5 189 2 546.6 0 2 190 2 546.6 0 2 191 6 546.6 0 6 192 1 546.6 0 1 193 8 546.6 0 8 194 8 546.6 0 8 195 6 546.6 0 6 196 7 546.6 0 7 197 16 546.6 0 16 198 14 546.6 0 14 199 41 546.6 0 41 200 48 546.6 0 48 201 69 546.6 0 69 202 27 546.6 0 27 203 19 546.6 0 19 204 77 546.6 0 77 205 22 546.6 0 22 206 15 546.6 0 15 207 12 546.6 0 12 208 13 546.6 0 13 209 13 546.6 0 13 210 5 546.6 0 5 211 5 546.6 0 5 212 11 546.6 0 11 213 14 546.6 0 14 214 10 546.6 0 10 215 10 546.6 0 10 216 5 546.6 0 5 217 21 546.6 0 21 218 47 546.6 0 47 219 53 546.6 0 53 220 12 546.6 0 12 221 13 546.6 0 13 222 205 546.6 0 205 223 66 546.6 0 66 224 250 546.6 0 250 225 23 546.6 0 23 226 7 546.6 0 7 227 7 546.6 0 7 228 7 546.6 0 7 229 13 546.6 0 13 230 6 546.6 0 6 231 8 546.6 0 8 232 9 546.6 0 9 233 12 546.6 0 12 234 7 546.6 0 7 235 10 546.6 0 10 236 15 546.6 0 15 237 10 546.6 0 10 238 17 546.6 0 17 239 16 546.6 0 16 240 27 546.6 0 27 241 16 546.6 0 16 242 17 546.6 0 17 243 15 546.6 0 15 244 16 546.6 0 16 245 11 546.6 0 11 246 8 546.6 0 8 247 10 546.6 0 10 248 13 546.6 0 13 249 11 546.6 0 11 250 10 546.6 0 10 251 30 546.6 0 30 252 7 546.6 0 7 253 11 546.6 0 11 254 10 546.6 0 10 255 19 546.6 0 19 256 28 546.6 0 28 257 38 546.6 0 38 258 23 546.6 0 23 259 17 546.6 0 17 260 16 546.6 0 16 261 11 546.6 0 11 262 11 546.6 0 11 263 28 546.6 0 28 264 29 546.6 0 29 265 21 546.6 0 21 266 19 546.6 0 19 267 9 546.6 0 9 268 13 546.6 0 13 269 15 546.6 0 15 270 11 546.6 0 11 271 19 546.6 0 19 272 15 546.6 0 15 273 24 546.6 0 24 274 10 546.6 0 10 275 13 546.6 0 13 276 25 546.6 0 25 277 16 546.6 0 16 278 30 546.6 0 30 279 10 546.6 0 10 280 9 546.6 0 9 281 15 546.6 0 15 282 14 546.6 0 14 283 18 546.6 0 18 284 28 546.6 0 28 285 21 546.6 0 21 286 21 546.6 0 21 287 18 546.6 0 18 288 33 546.6 0 33 289 29 546.6 0 29 290 20 546.6 0 20 291 31 546.6 0 31 292 42 546.6 0 42 293 19 546.6 0 19 294 21 546.6 0 21 295 12 546.6 0 12 296 18 546.6 0 18 297 21 546.6 0 21 298 24 546.6 0 24 299 27 546.6 0 27 300 21 546.6 0 21 301 23 546.6 0 23 Finished in 1.68 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 33,817 Reads with adapters: 12,524 (37.0%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 12,519 (37.0%) Total basepairs processed: 9,471,914 bp Total written (filtered): 3,453,287 bp (36.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12524 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 70 0.0 3 0 70 17 12381 0.0 3 12124 248 8 1 18 30 0.0 3 3 26 1 19 10 0.0 3 1 0 7 2 20 12 0.0 3 1 0 0 11 59 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 94 1 0.0 3 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 12,519 Reads with adapters: 106 (0.8%) Reads written (passing filters): 12,519 (100.0%) Total basepairs processed: 3,453,287 bp Total written (filtered): 3,452,922 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 106 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.7% C: 4.7% G: 38.7% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 59 195.6 0 59 4 47 48.9 0 47